| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,216,781 | G→A | M1M (ATG→ATA) † | nfeR → | DNA‑binding transcriptional repressor NfeR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,216,781 | 0 | G | A | 98.0% | 166.3 / ‑5.6 | 49 | M1M (ATG→ATA) † | nfeR | DNA‑binding transcriptional repressor NfeR |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (21/27); total (21/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTGCTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCACCAGCATGAACACGGTCAATGCTGCG > NC_000913/3216644‑3216908 | cGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATAAg > 2:470565/1‑140 (MQ=255) gATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATAAGCcatc > 2:977035/1‑140 (MQ=255) gATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATAAGCcatc < 2:477971/140‑1 (MQ=255) gATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATAAGCcatc < 2:440418/140‑1 (MQ=255) gATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATAAGCcatc > 1:440418/1‑140 (MQ=255) gAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATAAGCCATCATCACGAAgg < 2:263027/140‑1 (MQ=255) gAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATAAGCCATCATCACGAAgg < 1:787502/140‑1 (MQ=255) gAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATAAGCCATCATCACGAAgg < 1:764633/140‑1 (MQ=255) aCGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATAAGCCATCATCACGaa > 2:996640/1‑136 (MQ=255) aCGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATAAGCCATCATCACGaa < 1:996640/136‑1 (MQ=255) gCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATAAGCCATCATCACGAAGGGTGTTGTAAACATg > 1:199752/1‑140 (MQ=255) tACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATAAGCCATCATCACGAAGGGTGTTGTAAACATGAAg < 1:132009/140‑1 (MQ=255) cGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATAAGCCATCATCACGAAGGGTGTTGTAAACATGAAGgc > 1:768709/1‑140 (MQ=255) gagaTGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATAAGCCATCATCACGAAGGGTGTTGTAAACATGAAGgccagcc < 1:659620/140‑1 (MQ=255) gTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATAAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCAt > 2:468424/1‑140 (MQ=255) ccACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATAAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGa > 2:837459/1‑140 (MQ=255) gTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATAAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGgc < 1:468424/140‑1 (MQ=255) tttAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATAAGCCATCATCCCGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGgct < 2:350269/140‑1 (MQ=255) aaaaaTCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATAAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCTAGCCACGCCATGAGGgctgct < 2:768709/140‑1 (MQ=255) aaaaaTCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATAAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGgctgct > 1:348453/1‑140 (MQ=255) aaTCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATAAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTGCTGCa < 2:822040/140‑1 (MQ=255) aaTCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATAAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTGCTGCa < 2:199752/140‑1 (MQ=255) acacTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATAAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTGCTGCAAAGGTGAGaa < 1:479735/140‑1 (MQ=255) tGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATAAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTGCTGCAAAGGTGAGAAGTCAg > 2:18949/1‑140 (MQ=255) cGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATAAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTGCTGCAAAGGTGAGAAGTCAGAACACGAg < 1:470565/140‑1 (MQ=255) atCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATAAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTGCTGCAAAGGTGAGAAGTCAGAACACGAg > 2:916069/1‑133 (MQ=255) atCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATAAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTGCTGCAAAGGTGAGAAGTCAGAACACGAg < 1:916069/133‑1 (MQ=255) tCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATAAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTGCTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCgg < 2:1084158/140‑1 (MQ=255) cAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTGCTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGa < 1:531023/140‑1 (MQ=255) aGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATAAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTGCTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGac < 1:18949/140‑1 (MQ=255) atatAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATAAGCCATCATCACTAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTGCTGCAAAGGTGAGAAGTCAGAACACGAg < 2:754677/128‑1 (MQ=255) atatAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATAAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTGCTGCAAAGGTGAGAAGTCAGAACACGAg > 1:754677/1‑128 (MQ=255) aaTTAGATATATCTAATTAAGCAAAAGGAGGCTGATATAAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTGCTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCa < 1:888539/140‑1 (MQ=255) aaTTAGATATATCTAATTAAGCAAAAGGAGGCTGATATAAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTGCTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCa < 1:837459/140‑1 (MQ=255) aTTAGATATATCTAATTAAGCAAAAGGAGGCTGATATAAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTGCTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCAc < 2:482630/140‑1 (MQ=255) gATATATCTAATTAAGCAAAAGGAGGCTGATATAAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTGCTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCACCAGc > 1:703174/1‑140 (MQ=255) gATATATCTAATTAAGCAAAAGGAGGCTGATATAAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTGCTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCACCAGc > 1:327594/1‑140 (MQ=255) atatatCTAATTAAGCAAAAGGAGGCTGATATAAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTGCTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCACCAGCa < 2:1085712/140‑1 (MQ=255) atCTAATTAAGCAAAAGGAGGCTGATATAAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTGCTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCACCAGCATGaa > 1:568167/1‑140 (MQ=255) tCTAATTAAGCAAAAGGAGGCTGATATAAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTGCTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCACCAGCATGAac > 1:723945/1‑140 (MQ=255) cTAATTAAGCAAAAGGAGGCTGATATAAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTGCTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCACCAGCATGAaca > 2:551420/1‑140 (MQ=255) aTTAAGCAAAAGGAGGCTGATATAAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTGCTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCACCAGCATg > 1:186928/1‑133 (MQ=255) aTTAAGCAAAAGGAGGCTGATATAAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTGCTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCACCAGCATg < 2:186928/133‑1 (MQ=255) aaGCAAAAGGAGGCTGATATAAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTGCTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCACCAGCATGAACACGGTCa < 1:517898/140‑1 (MQ=255) aGCAAAAGGAGGCTGATATAAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTGCTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCACCAGCATGAACACGGTCaa > 1:800094/1‑140 (MQ=255) aGCAAAAGGAGGCTGATATAAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTGCTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCACCAGCATGAACACGGTCaa > 1:6652/1‑140 (MQ=255) cAAAAGGAGGCTGATATAAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTGCTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCACCAGCATGAACACGGt > 2:1052384/1‑135 (MQ=255) cAAAAGGAGGCTGATATAAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTGCTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCACCAGCATGAACACGGt < 1:1052384/135‑1 (MQ=255) ggaggCTGATATAAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTGCTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCACCAGCATGAGCACGGTCAATGCTGCg < 2:327594/140‑1 (MQ=255) | CGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTGCTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCACCAGCATGAACACGGTCAATGCTGCG > NC_000913/3216644‑3216908 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |