Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,254,443	T→C	V42A (GTG→GCG)	yhaK →	bicupin‑related protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,254,443	0	T	C	100.0%	311.0 / NA	86	V42A (GTG→GCG)	yhaK	bicupin‑related protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (45/41);  total (45/41)

AGGTCAAGAATTATGATTACTACCCGAACTGCCAGGCAGTGTGGACAAGCAGACTACGGATGGTTGCAGGCCCGGTATACTTTTTCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGTGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAATGTGATTCTGGATGGAGAAGCAGAGTATCGCGATAGCGAAGGCAATCATGTTCAGGCCAGCGC  >  NC_000913/3254307‑3254578                                                                                                                                         |                                                                                                                                        agGTCAAGAATTATGATTACTACCCGAACTGCCAGGCAGTGTGGACAAGCAGACTACGGATGGTTGCAGGCCCGGTATACTTTTTCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGCGCt                                                                                                                                      <  2:99129/140‑1 (MQ=255) agGTCAAGAATTATGATTACTACCCGAACTGCCAGGCAGTGTGGACAAGCAGACTACGGATGGTTGCAGGCCCGGTATACTTTTTCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGCGCt                                                                                                                                      <  2:724689/140‑1 (MQ=255) agGTCAAGAATTATGATTACTACCCGAACTGCCAGGCAGTGTGGACAAGCAGACTACGGATGGTTGCAGGCCCGGTATACTTTTTCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGCGCt                                                                                                                                      >  2:682979/1‑140 (MQ=255) agGTCAAGAATTATGATTACTACCCGAACTGCCAGGCAGTGTGGACAAGCAGACTACGGATGGTTGCAGGCCCGGTATACTTTTTCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGCGCt                                                                                                                                      <  2:189094/140‑1 (MQ=255)  ggTCAAGAATTATGATTACTACCCGAACTGCCAGGCAGTGTGGACAAGCAGACTACGGATGGTTGCAGGCCCGGTATACTTTTTCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGCGCtt                                                                                                                                     >  1:225090/1‑140 (MQ=255)   gTCAAGAATTATGATTACTACCCGAACTGCCAGGCAGTGTGGACAAGCAGACTACGGATGGTTGCAGGCCCGGTATACTTTTTCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGCGCTTa                                                                                                                                    >  1:336098/1‑140 (MQ=255)   gTCAAGAATTATGATTACTACCCGAACTGCCAGGCAGTGTGGACAAGCAGACTACGGATGGTTGCAGGCCCGGTATACTTTTTCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGCGCTTa                                                                                                                                    >  2:480804/1‑140 (MQ=255)    tCAAGAATTATGATTACTACCCGAACTGCCAGGCAGTGTGGACAAGCAGACTACGGATGGTTGCAGGCCCGGTATACTTTTTCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTgcgc                                                                                                                                       >  1:141186/1‑136 (MQ=255)    tCAAGAATTATGATTACTACCCGAACTGCCAGGCAGTGTGGACAAGCAGACTACGGATGGTTGCAGGCCCGGTATACTTTTTCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGCGCTTaa                                                                                                                                   >  1:680239/1‑140 (MQ=255)      aaGAATTATGATTACTACCCGAACTGCCAGGCAGTGTGGACAAGCAGACTACGGATGGTTGCAGGCCCGGTATACTTTTTCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGCGCTTAAcc                                                                                                                                 >  1:679764/1‑140 (MQ=255)         aaTTATGATTACTACCCGAACTGCCAGGCAGTGTGGACAAGCAGACTACGGATGGTTGCAGGCCCGGTATACTTTTTCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGCGCTTAACCAgg                                                                                                                              <  1:522608/140‑1 (MQ=255)          aTTATGATTACTACCCGAACTGCCAGGCAGTGTGGACAAGCAGACTACGGATGGTTGCAGGCCTGGTATACTTTTTCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGCGCTTAACCAGGa                                                                                                                             <  2:830547/140‑1 (MQ=255)           ttATGATTACTACCCGAACTGCCAGGCAGTGTGGACAAGCAGACTACGGATGGTTGCAGGCCCGGTATACTTTTTCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGCGCTTAACCAGGaa                                                                                                                            >  1:1069051/1‑140 (MQ=255)              tGATTACTACCCGAACTGCCAGGCAGTGTGGACAAGCAGACTACGGATGGTTGCAGGCCCGGTATACTTTTTCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGCGCTTAACCAGGAAGTg                                                                                                                         <  2:680239/140‑1 (MQ=255)                            aCTGCCAGGCAGTGTGGACAAGCAGACTACGGATGGTTGCAGGCCCGGTATACTTTTTCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTgc                                                                                                           <  1:805942/140‑1 (MQ=255)                            aCTGCCAGGCAGTGTGGACAAGCAGACTACGGATGGTTGCAGGCCCGGTATACTTTTTCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTgc                                                                                                           >  2:1081650/1‑140 (MQ=255)                                   ggCAGTGTGGACAAGCAGACTACGGATGGTTGCAGGCCCGGTATACTTTTTCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCttt                                                                                                    <  1:1081650/140‑1 (MQ=255)                                    gCAGTGTGGACAAGCAGACTACGGATGGTTGCAGGCCCGGTATACTTTTTCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCTTTc                                                                                                   >  2:417707/1‑140 (MQ=255)                                        tgtgGACAAGCAGACTACGGATGGTTGCAGGCCCGGTATACTTTTTCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCTTTCAGcc                                                                                               >  2:351185/1‑140 (MQ=255)                                        tgtgGACAAGCAGACTACGGATGGTTGCAGGCCCGGTATACTTTTTCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCTTTCAGcc                                                                                               >  2:606745/1‑140 (MQ=255)                                        tgtgGACAAGCAGACTACGGATGGTTGCAGGCCCGGTATACTTTTTCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCTTTCAGcc                                                                                               >  1:983187/1‑140 (MQ=255)                                         gtgGACAAGCAGACTACGGATGGTTGCAGGCCCGGTATACTTTTTCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCTTTCAGCcg                                                                                              <  1:317727/140‑1 (MQ=255)                                          tgGACAAGCAGACTACGGATGGTTGCAGGCCCGGTATACTTTTTCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCTTTCAGCcgc                                                                                             >  1:889224/1‑140 (MQ=255)                                          tgGACAAGCAGACTACGGATGGTTGCAGGCCCGGTATACTTTTTCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCTTTCAGCcgc                                                                                             <  1:682979/140‑1 (MQ=255)                                          tgGACAAGCAGACTACGGATGGTTGCAGGCCCGGTATACTTTTTCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCTTTCAGCcgc                                                                                             >  2:846974/1‑140 (MQ=255)                                          tgGACAAGCAGACTACGGATGGTTGCAGGCCCGGTATACTTTTTCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCTTTCAGCcgc                                                                                             >  1:88485/1‑140 (MQ=255)                                                  cAGACTACGGATGGTTGCAGGCCCGGTATACTTTTTCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTAt                                                                                     >  2:843984/1‑140 (MQ=255)                                                  cAGACTACGGATGGTTGCAGGCCCGGTATACTTTTTCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTAt                                                                                     >  1:465512/1‑140 (MQ=255)                                                  cAGACTACGGATGGTTGCAGGCCCGGTATACTTTTTCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTAt                                                                                     >  2:527579/1‑140 (MQ=255)                                                  cAGACTACGGATGGTTGCAGGCCCGGTATACTTTTTCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTAt                                                                                     >  2:824931/1‑140 (MQ=255)                                                  cAGACTACGGATGGTTGCAGGCCCGGTATACTTTTTCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTAt                                                                                     >  1:765429/1‑140 (MQ=255)                                                  cAGACTACGGATGGTTGCAGGCCCGGTATACTTTTTCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTAt                                                                                     >  1:885061/1‑140 (MQ=255)                                                  cAGACTACGGATGGTTGCAGGCCCGGTATACTTTTCCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTAt                                                                                     >  1:54213/1‑140 (MQ=255)                                                     aCTACGGATGGTTGCAGGCCCGGTATACTTTTTCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATccc                                                                                  <  1:480804/140‑1 (MQ=255)                                                         cGGATGGTTGCAGGCCCGGTATACTTTTTCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAg                                                                              >  2:552490/1‑140 (MQ=255)                                                           gATGGTTGCAGGCCCGGTATACTTTTTCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTc                                                                            <  1:846974/140‑1 (MQ=255)                                                             tGGTTGCAGGCCCGGTATACTTTTTCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGa                                                                          <  2:679764/140‑1 (MQ=255)                                                               gTTGCAGGCCCGGTATACTTTTTCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGata                                                                        >  2:416194/1‑140 (MQ=255)                                                               gTTGCAGGCCCGGTATACTTTTTCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGata                                                                        >  2:361186/1‑140 (MQ=255)                                                                   cAGGCCCGGTATACTTTTTCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATAtttt                                                                    >  1:818705/1‑140 (MQ=255)                                                                      gCCCGGTATACTTTTTCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTaaa                                                                 >  1:174987/1‑140 (MQ=255)                                                                        ccGGTATACTTTTTCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAAtg                                                               >  1:452912/1‑140 (MQ=255)                                                                           gTATACTTTTTCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAAtgtg                                                             >  2:521645/1‑139 (MQ=255)                                                                           gTATACTTTTTCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAAtgtg                                                             <  1:521645/139‑1 (MQ=255)                                                                             ataCTTTTTCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAATGTGAtt                                                          <  1:824931/140‑1 (MQ=255)                                                                             ataCTTTTTCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAATGTGAtt                                                          <  2:839403/140‑1 (MQ=255)                                                                             ataCTTTTTCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAATGTGAtt                                                          <  1:361186/140‑1 (MQ=255)                                                                                cTTTTTCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAATGTGATTCtg                                                       <  2:983187/140‑1 (MQ=255)                                                                                  ttttCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAATGTTATTCtgga                                                     <  2:933934/140‑1 (MQ=255)                                                                                  ttttCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAATGTGATTCtgga                                                     <  2:452912/140‑1 (MQ=255)                                                                                  ttttCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAATGTGATTCtgga                                                     >  1:515782/1‑140 (MQ=255)                                                                                    ttCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAATGTGATTCtggatg                                                   <  2:818705/140‑1 (MQ=255)                                                                                     tCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAATGTGATTCtggatgg                                                  <  2:465512/140‑1 (MQ=255)                                                                                       cTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAATGTGATTCTGGATGgag                                                <  1:843984/140‑1 (MQ=255)                                                                                       cTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAATGTGATTCTGGATGgag                                                <  1:120725/140‑1 (MQ=255)                                                                                       cTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAATGTGATTCTGGATGgag                                                <  2:889224/140‑1 (MQ=255)                                                                                       cTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAATGTGATTCTGGATGgag                                                <  1:606745/140‑1 (MQ=255)                                                                                          tGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAATGTGATTCTGGATGGAGAAg                                             <  2:632427/140‑1 (MQ=255)                                                                                            gACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAATGTGATTCTGGATGGAGAAGCa                                           <  2:246141/140‑1 (MQ=255)                                                                                            gACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAATGTGATTCTGGATGGAGAAGCa                                           <  2:765429/140‑1 (MQ=255)                                                                                                 tactTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAATGTGATTCTGGATGgag                                                >  2:153837/1‑130 (MQ=255)                                                                                                 tactTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAATGTGATTCTGGATGGAGAAGCAGAGTa                                      <  1:527579/140‑1 (MQ=255)                                                                                                 tactTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGACTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAATGTGATTCTGGATGgag                                                <  1:153837/130‑1 (MQ=255)                                                                                                  actTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAATGTGATTCTGGATGGAGAAGCAGAGTAt                                     <  1:417707/140‑1 (MQ=255)                                                                                                      cGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAATGTGATTCTGGATGGAGAAGCAGAGTATcgcg                                 <  2:515782/140‑1 (MQ=255)                                                                                                         cccGAAATTGTTAGGCTATGCCTCCCTGCGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAATGTGATTCTGGATGGAGAAGCAGAGTATCGCGATa                              >  2:941503/1‑140 (MQ=255)                                                                                                          ccGAAATTGTTAGGCTATGCCTCCCTGCGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAATGTGATTCTGGATGGAGAAGCAGAGTATCGCGATAg                             <  1:351185/140‑1 (MQ=255)                                                                                                             aaaTTGTTAGGCTATGCCTCCCTGCGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAATGTGATTCTGGATGGAGAAGCAGAGTATCGCGATAGCGa                          >  2:892227/1‑140 (MQ=255)                                                                                                                ttGTTAGGCTATGCCTCCCTGCGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAATGTGATTCTGGATGGAGAAGCAGAGTATCGCGATAGCGAAgg                       <  1:892227/140‑1 (MQ=255)                                                                                                                ttGTTAGGCTATGCCTCCCTGCGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAATGTGATTCTGGATGGAGAAGCAGAGTATCGCGATAGCGAAgg                       <  1:735162/140‑1 (MQ=255)                                                                                                                ttGTTAGGCTATGCCTCCCTGCGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAATGTGATTCTGGATGGAGAAGCAGAGTATCGCGATAGCGAAgg                       <  2:885061/140‑1 (MQ=255)                                                                                                                           tGCCTCCCTGCGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTGCTGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAATGTGATTCTGGATGGAGAAGCAGAGTATCGCGATAGCGAAGGCAATCATGTTc            >  1:960793/1‑140 (MQ=255)                                                                                                                           tGCCTCCCTGCGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCTAAGTCGATATTTTAAATGTGATTCTGGATGGAGAAGCAGAGTATCGCGATAGCGAAGGCAATCATGTTc            >  2:928765/1‑140 (MQ=255)                                                                                                                           tGCCTCCCTGCGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAATGTGATTCTGGATGGAGAAGCAGAGTATCGCGATAGCGAAgg                       >  1:919307/1‑129 (MQ=255)                                                                                                                           tGCCTCCCTGCGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAATGTGATTCTGGATGGAGAAGCAGAGTATCGCGATAGCGAAgg                       <  2:919307/129‑1 (MQ=255)                                                                                                                           tGCCTCCCTGCGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAATGTGATTCTGGATGGAGAAGCAGAGTATCGCGATAGCGAAGGCAATCATGTTc            >  1:89735/1‑140 (MQ=255)                                                                                                                           tGCCTCCCTGCGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAATGTGATTCTGGATGGAGAAGCAGAGTATCGCGATAGCGAAGGCAATCATGTTc            >  2:591515/1‑140 (MQ=255)                                                                                                                           tGCCTCCCTGCGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAATGTGATTCTGGATGGAGAAGCAGAGTATCGCGATAGCGAAGGCAATCATGTTc            >  2:834493/1‑140 (MQ=255)                                                                                                                           tGCCTCCCTGCGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAATGTGATTCTGGATGGAGAAGCAGAGTATCGCGATAGCGAAGGCAATCATGTTc            >  2:1076367/1‑140 (MQ=255)                                                                                                                               tCCCTGCGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAATGTGATTCTGGATGGAGAAGCAGAGTATCGCGATAGCGAAGGCAATCATGTTCAGGc        <  2:225090/140‑1 (MQ=255)                                                                                                                               tCCCTGCGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAATGTGATTCTGGATGGAGAAGCAGAGTATCGCGATAGCGAAGGCAATCATGTTCAGGc        <  2:89735/140‑1 (MQ=255)                                                                                                                                 ccTGCGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAATGTGATTCTGGATGGAGAAGCAGAGTATCGCGATAGCGAAGGCAATCATGTTCAGGCCa      >  1:529678/1‑140 (MQ=255)                                                                                                                                 ccTGCGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAATGTGATTCTGGATGGAGAAGCAGAGTATCGCGATAGCGAAGGCAATCATGTTCAGGCCa      >  2:685475/1‑140 (MQ=255)                                                                                                                                 ccTGCGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAATGTGATTCTGGATGGAGAAGCAGAGTATCGCGATAGCGAAGGCAATCATGTTCAGGCCa      >  2:593007/1‑140 (MQ=255)                                                                                                                                  cTGCGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAATGTGATTCTGGATGGAGAAGCAGAGTATCGCGATAGCGAAGGCAATCATGTTCAGGCCAg     <  1:685475/140‑1 (MQ=255)                                                                                                                                     cGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAATGTGATTCTGGATGGAGAAGCAGAGTATCGCGATAGCGAAGGCAATCATGTTCAGGCCAgcgc  >  1:566030/1‑140 (MQ=255)                                                                                                                                     cGTGCGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAATGTGATTCTGGATGGAGAAGCAGAGTATCGCGATAGCGAAGGCAATCATGTTCAGGCCAgcgc  >  1:1082652/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        AGGTCAAGAATTATGATTACTACCCGAACTGCCAGGCAGTGTGGACAAGCAGACTACGGATGGTTGCAGGCCCGGTATACTTTTTCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGTGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAATGTGATTCTGGATGGAGAAGCAGAGTATCGCGATAGCGAAGGCAATCATGTTCAGGCCAGCGC  >  NC_000913/3254307‑3254578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑