Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,350,177	A→C	F95V (TTT→GTT)	elbB ←	low activity glyoxalase ElbB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,350,177	0	A	C	100.0%	187.1 / NA	52	F95V (TTT→GTT)	elbB	low activity glyoxalase ElbB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (23/29);  total (23/29)

CGCCGGGGCAATACACATAAAACCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCA‑GCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAA‑ACCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGCACATTGCGCGTTTCCGTCATCGCTTCGCCAGTTAAATGG  >  NC_000913/3350043‑3350310                                                                                                                                         |                                                                                                                                      cGCCGGGGCAATACACATAAAACCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCA‑GCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAA‑cccccc                                                                                                                                  <  1:260711/140‑1 (MQ=255)    gggggAAATACCCATAAAACCAAGCGGTTTCCCGGCCTGATCCATGGC‑TGTGGCAGGCGCCTTTAATTCACGGTCAACGTGGCATTCGTTCCCAAGACTGGAAAAATGGCTTAAATTCTTCGCCGCGCC‑A‑ACCCCCCCgg                                                                                                                              <  2:767751/139‑1 (MQ=255)        gCAATACACATAAAACCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCA‑GCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAA‑CCCCCCCCGGca                                                                                                                            <  2:191692/139‑1 (MQ=255)        gCAATACACATAAAACCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCA‑GCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAA‑CCCCCCCCGGca                                                                                                                            >  1:191692/1‑139 (MQ=255)               acaTAAAACCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCA‑GCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAA‑CCCCCCCCGGCACAATCAAc                                                                                                                    <  1:857105/140‑1 (MQ=255)                 aTAAAACCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCA‑GCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAA‑CCCCCCCCGGCACAATCAACGc                                                                                                                  >  2:352281/1‑140 (MQ=255)                 aTAAAACCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCA‑GCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAA‑CCCCCCCCGGCACAATCAACGc                                                                                                                  >  1:432115/1‑140 (MQ=255)                 aTAAAACCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCA‑GCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAA‑CCCCCCCCGGCACAATCAACGc                                                                                                                  >  1:622397/1‑140 (MQ=255)                  tAAAACCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCA‑GCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAA‑CCCCCCCCGGCACAATCAACGCa                                                                                                                 <  2:59688/140‑1 (MQ=255)                      aCCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCA‑GCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAA‑CCCCCCCCGGCACAATCAACGCATCCa                                                                                                             >  2:1047202/1‑140 (MQ=255)                      aCCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCA‑GCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAA‑CCCCCCCCGGCACAATCAACGCATCCa                                                                                                             >  2:518775/1‑140 (MQ=255)                         aaGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCA‑GCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAA‑CCCCCCCCGGCACAATCAACGCATCCAGtt                                                                                                          >  1:923699/1‑140 (MQ=255)                                  tCCGGCCTGATGCATCGCTTGTGCCA‑GCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAA‑CCCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCAt                                                                                                 <  2:432115/140‑1 (MQ=255)                                  tCCGGCCTGATGCATCGCTTGTGCCA‑GCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAA‑CCCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCAt                                                                                                 <  1:593403/140‑1 (MQ=255)                                      gCCTGATGCATCGCTTGTGCCA‑GCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAA‑CCCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGc                                                                                             <  2:474142/140‑1 (MQ=255)                                      gCCTGATGCATCGCTTGTGCCA‑GCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAA‑CCCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGc                                                                                             <  1:745853/140‑1 (MQ=255)                                      gCCTGATGCATCGCTTGTGCCA‑GCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAA‑CCCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGc                                                                                             <  2:93207/140‑1 (MQ=255)                                      gCCTGATGCATCGCTTGTGCCA‑GCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAA‑CCCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGc                                                                                             <  2:615732/140‑1 (MQ=255)                                      gCCTGATGCATCGCTTGTGCCA‑GCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAA‑CCCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGc                                                                                             <  2:522984/140‑1 (MQ=255)                                           aTGCATCGCTTGTGCCA‑GCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAA‑CCCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTg                                                                                          <  1:1048329/138‑1 (MQ=255)                                           aTGCATCGCTTGTGCCA‑GCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAA‑CCCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTg                                                                                          >  2:1048329/1‑138 (MQ=255)                                            tGCATCGCTTGTGCCA‑GCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAA‑CCCCCCCCGACACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGc                                                                                       <  1:305991/140‑1 (MQ=255)                                             gCATCGCTTGTGCCA‑GCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAA‑CCCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGcc                                                                                      <  2:90592/140‑1 (MQ=255)                                                     tgtgCCA‑GCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAA‑CCCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACg                                                                              >  2:882808/1‑140 (MQ=255)                                                     tgtgCCA‑GCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAA‑CCCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACg                                                                              >  1:960163/1‑140 (MQ=255)                                                               gcCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAA‑CCCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCAcc                                                                     <  1:136710/140‑1 (MQ=255)                                                                         cACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAA‑CCCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTAtt                                                           <  1:882808/140‑1 (MQ=255)                                                                             gTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAA‑CCCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTg                                                       <  1:1047202/140‑1 (MQ=255)                                                                             gTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAA‑CCCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTg                                                       <  2:421750/140‑1 (MQ=255)                                                                                aaCGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAA‑CCCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCg                                                    >  1:702866/1‑140 (MQ=255)                                                                                  cGGTGCATTTGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAA‑CCCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCt                                                  <  1:518775/140‑1 (MQ=255)                                                                                      gCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAA‑CCCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCaa                                              >  2:1047949/1‑140 (MQ=255)                                                                                         ttCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAA‑CCCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCa                                           <  2:702866/140‑1 (MQ=255)                                                                                          tCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAACCCCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCt                                                  <  2:331385/133‑1 (MQ=255)                                                                                          tCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAACCCCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCt                                                  >  1:331385/1‑133 (MQ=255)                                                                                          tCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAA‑CCCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAg                                          >  1:648034/1‑140 (MQ=255)                                                                                            gCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAA‑CCCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGca                                        >  2:536240/1‑140 (MQ=255)                                                                                              tACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAA‑CCCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGcaca                                      <  1:536240/140‑1 (MQ=255)                                                                                                   aGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAA‑CCCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGCACATTgcg                                 >  2:667574/1‑140 (MQ=255)                                                                                                     aCTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAA‑CCCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGCACATTGcgcg                               >  2:974596/1‑140 (MQ=255)                                                                                                        ggCAAAATTGCTTAAATTCTTCGCCGCGCCAA‑CCCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGTCCAGAGGACGGATTTCACCGCGTGTTATTCGTGCCGCTTCAATCAGCACATTGCGCGttt                            >  2:198097/1‑140 (MQ=255)                                                                                                          cAAAATTGCTTAAATTCTTCGCCGCGCCAA‑CCCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCGGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGCACATTGCGCGTTTcc                          <  2:622397/140‑1 (MQ=255)                                                                                                          cAAAATTGCTTAAATTCTTCGCCGCGCCAA‑CCCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGCACATTGCGCGTTTcc                          >  2:1060800/1‑140 (MQ=255)                                                                                                          cAAAATTGCTTAAATTCTTCGCCGCGCCAA‑CCCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGCACATTGCGCGTTTcc                          >  1:648605/1‑140 (MQ=255)                                                                                                                 gCTTAAATTCTTCGCCGCGCCAA‑CCCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGCACATTGCGCGTTTCCGTCATCg                   <  2:648034/140‑1 (MQ=255)                                                                                                                    tAAATTCTTCGCCGCGCCAA‑CCCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGCACATTGCGCGTTTCCGTCATCGCtt                >  2:876319/1‑140 (MQ=255)                                                                                                                       aTTCTTCGCCGCGCCAA‑CCCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGCACATTGCGCGTTTCCGTCATCGCTTCGc             <  1:1060800/140‑1 (MQ=255)                                                                                                                             cgccgcGCCAACCCCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGCACATTGCGCGTTTCCGTCATCGCTTCGCCAGtt        <  2:570204/140‑1 (MQ=255)                                                                                                                             cgccgcGCCAA‑CCCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGCACATTGCGCGTTTCCGTCATCGCTTCGCCAGTTa       >  1:441444/1‑140 (MQ=255)                                                                                                                              gccgcGCCAA‑CCCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGCACATTGCGCGTTTCCGTCATCGCTTCGCCAGTTaa      <  1:1047949/140‑1 (MQ=255)                                                                                                                               ccgcGCCAA‑CCCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGCACATTGCGCGTTTCCGTCATCGCTTCGCCAGTTaaa     <  2:239500/140‑1 (MQ=255)                                                                                                                                 gcgcCAA‑CCCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGCACATTGCGCGTTTCCGTCATCGCTTCGCCAGTTAAATg   <  1:352281/140‑1 (MQ=255)                                                                                                                                  cgcCAA‑CCCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGCACATTGCGCGTTTCCGTCATCGCTTCGCCAGTTAAATgg  >  1:510391/1‑140 (MQ=255)                                                                                                                                  cgcCAA‑CCCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGCACATTGCGCGTTTCCGTCATCGCTTCGCCAGTTAAATgg  <  2:923699/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                      CGCCGGGGCAATACACATAAAACCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCA‑GCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAA‑ACCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGCACATTGCGCGTTTCCGTCATCGCTTCGCCAGTTAAATGG  >  NC_000913/3350043‑3350310

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑