Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,385,512	T→C	intergenic (+339/‑26)	argR → / → yhcN	DNA‑binding transcriptional dual regulator ArgR/DUF1471 domain‑containing stress‑induced protein YhcN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,385,512	0	T	C	100.0%	198.0 / NA	55	intergenic (+339/‑26)	argR/yhcN	DNA‑binding transcriptional dual regulator ArgR/DUF1471 domain‑containing stress‑induced protein YhcN
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/21);  total (34/21)

ATTTTTAACACTTAGTATCAACTGAAACAGTTAGCGCGGTATTAATTAGCTCAATAATTAGTGTATACTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCTAAAAGATTA‑‑‑‑‑TGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGTTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAAT  >  NC_000913/3385406‑3385647                                                                                                           |                                                                                                                                             aTTTTTAACACTTAGTATCAACTGAAACAGTTAGCGCGGTATTAATTAGCTCAATAATTAGTGTATACTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCCAAAAGATTA‑‑‑‑‑TGTCGAGGTAAAAATCATGAAAAt                                                                                                        >  2:789354/1‑140 (MQ=255)              aGTATCAACTGAAACAGTTAGCGCGGTATTAATTAGCTCAATAATTAGTGTATACTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCCAAAAGATTA‑‑‑‑‑TGTCGAGGTAAAAATCATGAAAATCAAAACCACTGtt                                                                                           >  1:1053550/1‑140 (MQ=255)              aGTATCAACTGAAACAGTTAGCGCGGTATTAATTAGCTCAATAATTAGTGTATACTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCCAAAAGATTA‑‑‑‑‑TGTCGAGGTAAAAATCATGAAAATCAAAACCACTGtt                                                                                           >  2:617084/1‑140 (MQ=255)                     aCTGAAACAGTTAGCGCGGTATTAATTAGCTCAATAATTAGTGTATACTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCCAAAAGATTA‑‑‑‑‑TGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCAt                                                                                    <  1:1046805/140‑1 (MQ=255)                     aCTGAAACAGTTAGCGCGGTATTAATTAGCTCAATAATTAGTGTATACTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCCAAAAGATTA‑‑‑‑‑TGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCAt                                                                                    >  2:1050215/1‑140 (MQ=255)                     aCTGAAACAGTTAGCGCGGTATTAATTAGCTCAATAATTAGTGTATACTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCCAAAAGATTA‑‑‑‑‑TGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCAt                                                                                    >  1:955573/1‑140 (MQ=255)                     aCTGAAACAGTTAGCGCGGTATTAATTAGCTCAATAATTAGTGTATACTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCCAAAAGATTA‑‑‑‑‑TGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCAt                                                                                    <  2:955573/140‑1 (MQ=255)                      cTGAAACAGTTAGCGCGGTATTAATTAGCTCAATAATTAGTGTATACTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCCAAAAGATTA‑‑‑‑‑TGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCAtt                                                                                   >  1:557550/1‑140 (MQ=255)                       tGAAACAGTTAGCGCGGTATTAATTAGCTCAATAATTAGTGTATACTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCCAAAAGATTA‑‑‑‑‑TGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTa                                                                                  <  1:935466/140‑1 (MQ=255)                        gAAACAGTTAGCGCGGTATTAATTAGCTCAATAATTAGTGTATACTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCCAAAAGATTA‑‑‑‑‑TGTCGAGGTAAAAATCATGAAAATCAAAACCAc                                                                                               >  1:439175/1‑126 (MQ=255)                        gAAACAGTTAGCGCGGTATTAATTAGCTCAATAATTAGTGTATACTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCCAAAAGATTA‑‑‑‑‑TGTCGAGGTAAAAATCATGAAAATCAAAACCAc                                                                                               <  2:439175/126‑1 (MQ=255)                          aaCAGTTAGCGCGGTATTAATTAGCTCAATAATTAGTGTATACTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCCAAAAGATTATGTCCTGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCAt                                                                                    <  2:501017/140‑1 (MQ=255)                          aaCAGTTAGCGCGGTATTAATTAGCTCAATAATTAGTGTATACTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCCAAAAGATTA‑‑‑‑‑TGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGc                                                                               >  2:592120/1‑140 (MQ=255)                               ttAGCGCGGTATTAATTAGCTCAATAATTAGTGTATACTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCCAAAAGATTA‑‑‑‑‑TGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTtg                                                                                          >  1:486719/1‑124 (MQ=255)                               ttAGCGCGGTATTAATTAGCTCAATAATTAGTGTATACTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCCAAAAGATTA‑‑‑‑‑TGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTtg                                                                                          <  2:486719/124‑1 (MQ=255)                                   cgcgGTATTAATTAGCTCAATAATTAGTGTATACTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCCAAAAGATTA‑‑‑‑‑TGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCt                                                                      >  2:316410/1‑140 (MQ=255)                                              ttaGCTCAATAATTAGTGTATACTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCCAAAAGATTA‑‑‑‑‑TGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGTTCTCTCttt                                                           <  2:284653/140‑1 (MQ=255)                                                  cTCAATAATTAGTGTATACTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCCAAAAGATTA‑‑‑‑‑TGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGTtctc                                                                <  1:971245/131‑1 (MQ=255)                                                  cTCAATAATTAGTGTATACTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCCAAAAGATTA‑‑‑‑‑TGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGTtctc                                                                >  2:971245/1‑131 (MQ=255)                                                  cTCAATAATTAGTGTATACTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCCAAAAGATTA‑‑‑‑‑TGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGTTCTCTCTTTCGGt                                                       >  1:296503/1‑140 (MQ=255)                                                  cTCAATAATTAGTGTATACTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCCAAAAGATTA‑‑‑‑‑TGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGTTCTCTCTTTCGGt                                                       >  2:89621/1‑140 (MQ=255)                                                  cTCAATAATTAGTGTATACTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCCAAAAGATTA‑‑‑‑‑TGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGTTCTCTCTTTCGGt                                                       >  2:69410/1‑140 (MQ=255)                                                  cTCAATAATTAGTGTATACTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCCAAAAGATTA‑‑‑‑‑TGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGTTCTCTCTTTCGGt                                                       >  1:1053113/1‑140 (MQ=255)                                                  cTCAATAATTAGTGTATACTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCCAAAAGATTA‑‑‑‑‑TGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGTTCTCTCTTTCGGt                                                       >  2:495802/1‑140 (MQ=255)                                                  cTCAATAATTAGTGTATACTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCCAAAAGATTA‑‑‑‑‑TGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGTTCTCTCTTTCGGt                                                       >  2:554117/1‑140 (MQ=255)                                                  cTCAATAATTAGTGTATACTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCCAAAAGATTA‑‑‑‑‑TGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGTTCTCTCTTTCGGt                                                       >  2:22827/1‑140 (MQ=255)                                                  cTCAATAATTAGTGTATACTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCCAAAAGATTA‑‑‑‑‑TGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGTTCTCTCTTTCGGt                                                       >  2:83820/1‑140 (MQ=255)                                                      ataatTAGTGTATACTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCCAAAAGATTA‑‑‑‑‑TGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGTTCTCTCTTTCGGTGCAt                                                   >  2:570225/1‑140 (MQ=255)                                                       taatTAGTGTATACTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCCAAAAGATTA‑‑‑‑‑TGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGTTCTCTCTTTCg                                                         <  1:400572/133‑1 (MQ=255)                                                       taatTAGTGTATACTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCCAAAAGATTA‑‑‑‑‑TGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGTTCTCTCTTTCg                                                         >  2:400572/1‑133 (MQ=255)                                                       taatTAGTGTATACTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCCAAAAGATTA‑‑‑‑‑TGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGTTCTCTCTTTCGGTGCAtt                                                  <  2:356538/140‑1 (MQ=255)                                                       taatTAGTGTATACTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCCAAAAGATTA‑‑‑‑‑TGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGTTCTCTCTTTCGGTGCAtt                                                  <  1:89621/140‑1 (MQ=255)                                                          ttAGTGTATACTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCCAAAAGATTA‑‑‑‑‑TGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGTTCTCTCTTTCGGTGCATTCGc                                               >  1:180962/1‑140 (MQ=255)                                                            aGTGTATACTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCCAAAAGATTA‑‑‑‑‑TGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGTTCTCTCTTTCGGTGCATTCGCTg                                             >  1:324156/1‑140 (MQ=255)                                                              tgtATACTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCCAAAAGATTA‑‑‑‑‑TGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGTTCTCTCTTTCGGTGCATTCGCTGcc                                           <  1:1050215/140‑1 (MQ=255)                                                              tgtATACTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCCAAAAGATTA‑‑‑‑‑TGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGTTCTCTCTTTCGGTGCATTCGCTGcc                                           <  1:83820/140‑1 (MQ=255)                                                                tataCTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCCAAAAGATTA‑‑‑‑‑TGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGTTCTCTCTTTCGGTGCATTCGCTGCCGa                                         <  2:302635/140‑1 (MQ=255)                                                                 ataCTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCCAAAAGATTA‑‑‑‑‑TGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGTTCTCTCTTTCGGTGCATTCGCTGCCGAc                                        >  2:888715/1‑140 (MQ=255)                                                                 ataCTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCCAAAAGATTA‑‑‑‑‑TGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGTTCTCTCTTTCGGTGCATTCGCTGCCGAc                                        >  1:99600/1‑140 (MQ=255)                                                                 ataCTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCCAAAAGATTA‑‑‑‑‑TGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGTTCTCTCTTTCGGTGCATTCGCTGCCGAc                                        <  2:1053550/140‑1 (MQ=255)                                                                  taCTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCCAAAAGATTA‑‑‑‑‑TGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGTTCTCTCTTTCGGTGCATTCGCTGCCGACt                                       >  1:398074/1‑140 (MQ=255)                                                                      tGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCCAAAAGATTA‑‑‑‑‑TGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGTTCTCTCTTTCGGTGCATTCGCTGCCGACTCCAt                                   <  2:974314/140‑1 (MQ=255)                                                                       gATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCCAAAAGATTA‑‑‑‑‑TGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGTTCTCTCTTTCGGTGCATTCGCTGCCGa                                         <  2:445850/133‑1 (MQ=255)                                                                       gATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCCAAAAGATTA‑‑‑‑‑TGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGTTCTCTCTTTCGGTGCATTCGCTGCCGa                                         >  1:445850/1‑133 (MQ=255)                                                                        aTTTTGTGATATGGGTCACGAAACAAAGGCCCAGCCAAAAGATTA‑‑‑‑‑TGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGTTCTCTCTTTCGGTGCATTCGCTGCCGAc                                        >  2:17218/1‑133 (MQ=255)                                                                        aTTTTGTGATATGGGTCACGAAACAAAGGCCCAGCCAAAAGATTA‑‑‑‑‑TGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGTTCTCTCTTTCGGTGCATTCGCTGCCGAc                                        <  1:17218/133‑1 (MQ=255)                                                                          tttGTGATATGGGTCACGAAACAAAGGCCCAGCCAAAAGATTA‑‑‑‑‑TGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGTTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGAt                               <  2:557550/140‑1 (MQ=255)                                                                            tgtgATATGGGTCACGAAACAAAGGCCCAGCCAAAAGATTA‑‑‑‑‑TGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGTTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGAtgc                             >  2:168156/1‑140 (MQ=255)                                                                            tgtgATATGGGTCACGAAACAAAGGCCCAGCCAAAAGATTA‑‑‑‑‑TGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGTTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGAtgc                             >  1:998806/1‑140 (MQ=255)                                                                            tgtgATATGGGTCACGAAACAAAGGCCCAGCCAAAAGATTA‑‑‑‑‑TGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGTTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGAtgc                             >  2:36869/1‑140 (MQ=255)                                                                                   tGGGTCACGAAACAAAGGCCCAGCCAAAAGATTA‑‑‑‑‑TGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGTTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTgcacaa                      >  2:587681/1‑140 (MQ=255)                                                                                     ggTCACGAAACAAAGGCCCAGCCAAAAGATTA‑‑‑‑‑TGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGTTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTgcacaagc                    >  2:401577/1‑140 (MQ=255)                                                                                        cACGAAACAAAGGCCCAGCCAAAAGATTA‑‑‑‑‑TGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGTTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTgcacaagcaca                 >  2:896473/1‑140 (MQ=255)                                                                                                   ggCCCAGCCAAAAGATTA‑‑‑‑‑TGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGTTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAGAATCGTGAAg      <  1:554117/140‑1 (MQ=255)                                                                                                       cAGCCAAAAGATTA‑‑‑‑‑TGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGTTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAAt  <  2:99600/140‑1 (MQ=255)                                                                                                           |                                                                                                                                             ATTTTTAACACTTAGTATCAACTGAAACAGTTAGCGCGGTATTAATTAGCTCAATAATTAGTGTATACTTGATTTTGTGATATGGGTCACGAAACAAAGGCCCAGCTAAAAGATTA‑‑‑‑‑TGTCGAGGTAAAAATCATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGTTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAAT  >  NC_000913/3385406‑3385647

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑