Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,060,843	(G)6→5	coding (397/711 nt)	yihL →	putative transcriptional regulator YihL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,060,838	0	G	.	100.0%	205.3 / NA	48	coding (392/711 nt)	yihL	putative transcriptional regulator YihL
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (20/28);  total (20/28)

TTCAGCGGGCCGCAATTGAGCAAGGGCGAGAGCCTTCCTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAAGAGACGG  >  NC_000913/4060700‑4060972                                                                                                                                           |                                                                                                                                       ttCAGCGGGCCGCAATTGAGCAAGGGCGAGAGCCTTCCTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTgg                                                                                                                                       >  1:75578/1‑140 (MQ=255) ttCAGCGGGCCGCAATTGAGCAAGGGCGAGAGCCTTCCTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTgg                                                                                                                                       <  1:795986/140‑1 (MQ=255) ttCAGCGGGCCGCAATTGAGCAAGGGCGAGAGCCTTCCTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTgg                                                                                                                                       <  1:130593/140‑1 (MQ=255)  tCAGCGGGCCGCAATTGAGCAAGGGCGAGAGCCTTCCTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTggg                                                                                                                                      <  1:633347/140‑1 (MQ=255)  tCAGCGGGCCGCAATTGAGCAAGCTCTAGAGAATTTCTGGGGGTTTACCGAGAAAAACCGTGCCAGCGATATTCCCGAGACGCTCGCGCCTCTGATTGCAGTTTTGCCATCAACTGAACTCTATCGCAGCACCGGCTggg                                                                                                                                      <  2:192764/140‑1 (MQ=255)    aGCGGGCCGCAATTGAGCAAGGGCGAGAGCCTTCCTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCTTCACCGGCTggggg                                                                                                                                    <  2:473547/140‑1 (MQ=255)                ttGAGCAAGGGCGAGAGCCTTCCTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCT‑GGGGGCGCTGGAAGGAc                                                                                                                       <  1:709481/140‑1 (MQ=255)                   aGCAAGGGCGAGAGCCTTCCTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCT‑GGGGGCGCTGGAAGGACATa                                                                                                                    >  1:233367/1‑140 (MQ=255)                   aGCAAGGGCGAGAGCCTTCCTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCT‑GGGGGCGCTGGAAGGACATa                                                                                                                    >  2:64508/1‑140 (MQ=255)                          gCGAGAGCCTTCCTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCT‑GGGGGCGCTGGAAGGACATAAAGtttt                                                                                                             <  2:81245/140‑1 (MQ=255)                             agagCCTTCCTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCT‑GGGGGCGCTGGAAGGACATAAAGTTTTCTa                                                                                                          >  2:595166/1‑140 (MQ=255)                                gCCTTCCTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCT‑GGGGGCGCTGGAAGGACATAAAGTTTTCTATCa                                                                                                       <  2:342615/140‑1 (MQ=255)                                 ccTTCCTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCT‑GGGGGCGCTGGAAGGACATAAAGTTTTCTATCAc                                                                                                      <  1:64508/140‑1 (MQ=255)                                 ccTTCCTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCT‑GGGGGCGCTGGAAGGACATAAAGTTTTCTATCAc                                                                                                      <  2:749331/140‑1 (MQ=255)                                  cTTCCTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCT‑GGGGGCGCTGGAAGGACATAAGGTTTTCTATCACg                                                                                                     <  2:690558/140‑1 (MQ=255)                                  cTTCCTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCT‑GGGGGCGCTGGAAGGACATAAAGTTTTCTATCACg                                                                                                     <  1:493027/140‑1 (MQ=255)                                      cTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCT‑GGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAAc                                                                                                 <  2:164438/140‑1 (MQ=255)                                      cTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCT‑GGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAAc                                                                                                 <  2:856257/140‑1 (MQ=255)                                          gggTTTACCGAGAAAAACCGTACAAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCT‑GGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACatat                                                                                             >  1:221196/1‑140 (MQ=255)                                             tttACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCT‑GGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATAtt                                                                                          <  2:41999/140‑1 (MQ=255)                                             tttACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCT‑GGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATAtt                                                                                          <  2:135658/140‑1 (MQ=255)                                               tACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCT‑GGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAAc                                                                                                 <  2:28766/131‑1 (MQ=255)                                               tACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCT‑GGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAAc                                                                                                 >  1:28766/1‑131 (MQ=255)                                               tACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCT‑GGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTaa                                                                                        >  1:467100/1‑140 (MQ=255)                                               tACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCT‑GGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTaa                                                                                        >  2:81451/1‑140 (MQ=255)                                               tACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCT‑GGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTaa                                                                                        >  1:817582/1‑140 (MQ=255)                                                   gagaAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCT‑GGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCt                                                                                    >  2:462652/1‑140 (MQ=255)                                                       aaaaCCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCT‑GGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAg                                                                                <  2:233367/140‑1 (MQ=255)                                                           ccGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCT‑GGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGc                                                                            >  1:807342/1‑140 (MQ=255)                                                                 cAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCT‑GGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCggg                                                                      <  1:600909/140‑1 (MQ=255)                                                                         ttCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCT‑GGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGt                                                                               <  1:293446/123‑1 (MQ=255)                                                                         ttCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCT‑GGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGt                                                                               >  2:293446/1‑123 (MQ=255)                                                                            ccGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCT‑GGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGaac                                                           <  2:817582/140‑1 (MQ=255)                                                                            ccGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCT‑GGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGaac                                                           <  2:75578/140‑1 (MQ=255)                                                                                 aCGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCT‑GGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACtt                                                      >  1:327075/1‑140 (MQ=255)                                                                                  cGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCT‑GGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTg                                                              >  1:842022/1‑131 (MQ=255)                                                                                  cGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCT‑GGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTg                                                              <  2:842022/131‑1 (MQ=255)                                                                                    cTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCT‑GGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGaa                                                   >  1:231698/1‑140 (MQ=255)                                                                                    cTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCT‑GGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGaa                                                   >  2:450280/1‑140 (MQ=255)                                                                                     tCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCT‑GGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGaaa                                                  <  2:807342/140‑1 (MQ=255)                                                                                          ccACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCT‑GGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCAc                                             <  2:531587/140‑1 (MQ=255)                                                                                                    cAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCT‑GGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTg                                   <  1:450280/140‑1 (MQ=255)                                                                                                    cAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCT‑GGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTg                                   <  1:662934/140‑1 (MQ=255)                                                                                                    cAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCT‑GGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTg                                   <  2:231698/140‑1 (MQ=255)                                                                                                    cAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCT‑GGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTg                                   <  1:595166/140‑1 (MQ=255)                                                                                                     aGTGACGCCATCAACTGAACTCTATCGCATCACCGGCT‑GGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGc                                  <  1:603018/140‑1 (MQ=255)                                                                                                          cGCCATCAACTGAACTCTATCGCATCACCGGCT‑GGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCt                             >  2:734836/1‑140 (MQ=255)                                                                                                          cGCCATCAACTGAACTCTATCGCATCACCGGCT‑GGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCt                             >  1:238699/1‑140 (MQ=255)                                                                                                          cGCCATCAACTGAACTCTATCGCATCACCGGCT‑GGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCt                             >  1:829011/1‑140 (MQ=255)                                                                                                              aTCAACTGAACTCTATCGCATCACCGGCT‑GGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGa                         <  2:221196/140‑1 (MQ=255)                                                                                                                             tCGCATCACCGGCT‑GGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCaaaa          >  2:137046/1‑140 (MQ=255)                                                                                                                                  tCACCGGCT‑GGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAAgaga     >  2:617059/1‑140 (MQ=255)                                                                                                                                  tCACCGGCT‑GGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGAAGTCTGGGAAAAGTGCTACCAAAAAgaga     <  1:81451/140‑1 (MQ=255)                                                                                                                                     ccGGCT‑GGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAAGAGACgg  <  2:829011/140‑1 (MQ=255)                                                                                                                                           |                                                                                                                                       TTCAGCGGGCCGCAATTGAGCAAGGGCGAGAGCCTTCCTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAAGAGACGG  >  NC_000913/4060700‑4060972

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑