Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,330,519	(A)6→5	coding (18/1503 nt)	proP →	osmolyte:H(+) symporter ProP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,330,514	0	A	.	100.0%	253.9 / NA	59	coding (13/1503 nt)	proP	osmolyte:H(+) symporter ProP
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (23/36);  total (23/36)

GAGGGTGTAAGCAAACCCGCTACGCTTGTTACAGAGATTGCATCCTGCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTTTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGGAAAAAAGTAAAACCGATTACCCTTCGTGATGTCACCATTATTGATGACGGTAAACTGCGTAAAGCCATTACCGCAGCATCACTGGGTAATGCAATGGAATGGTTCGATTTTGGTGTTTATGGTTTTGTTGCTT  >  NC_000913/4330375‑4330646                                                                                                                                            |                                                                                                                                     gAGGGTGTAAGCAAACCCGCTACGCTTGTTACAGAGATTGCATCCTGCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTTTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGGa                                                                                                                                      >  1:818320/1‑140 (MQ=255) gAGGGTGTAAGCAAACCCGCTACGCTTGTTACAGAGATTGCATCCTGCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTTTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGGa                                                                                                                                      <  2:818320/140‑1 (MQ=255) gAGGGTGTAAGCAAACCCGCTACGCTTGTTACAGAGATTGCATCCTGCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTTTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGGa                                                                                                                                      >  2:395100/1‑140 (MQ=255) gAGGGTGTAAGCAAACCCGCTACGCTTGTTACAGAGATTGCATCCTGCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTTTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGGa                                                                                                                                      >  2:665368/1‑140 (MQ=255) gAGGGTGTAAGCAAACCCGCTACGCTTGTTACAGAGATTGCATCCTGCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTTTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGGa                                                                                                                                      >  2:488633/1‑140 (MQ=255)       gtAAGCAAACCCGCTACGCTTGTTACAGAGATTGCATCCTGCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTTTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGGaaaaagt                                                                                                                                <  1:488633/140‑3 (MQ=255)        tAAGCAAACCCGCTACGCTTGTTACAGAGATTGCATCCTGCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTTTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGGaaaaagta                                                                                                                               >  1:752699/1‑137 (MQ=255)               aCCCGCTACGCTTGTTACAGAGATTGCATCCTGCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTTTTCTAGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGG‑AAAAAGTAAAACCGa                                                                                                                       >  2:17843/1‑140 (MQ=255)                cccGCTACGCTTGTTACAGAGATTGCATCCTGCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTTTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGG‑AAAAAGTAAAACCGAt                                                                                                                      <  1:682578/140‑1 (MQ=255)                cccGCTACGCTTGTTACAGAGATTGCATCCTGCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTTTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGG‑AAAAAGTAAAACCGAt                                                                                                                      >  1:347647/1‑140 (MQ=255)                  cGCTACGCTTGTTACAGAGATTGCATCCTGCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTTTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGG‑AAAAAGTAAAACCGATTa                                                                                                                    <  1:395100/140‑1 (MQ=255)                  cGCTACGCTTGTTACAGAGATTGCATCCTGCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTTTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGG‑AAAAAGTAAAACCGATTa                                                                                                                    >  2:836756/1‑140 (MQ=255)                   gCTACGCTTGTTACAGAGATTGCATCCTGCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTTTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGG‑AAAAAGTAAAACCGATTAc                                                                                                                   <  2:701343/140‑1 (MQ=255)                    cTACGCTTGTTACAGAGATTGCATCCTGCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTTTTCTAGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGG‑AAAAAGTAAAACCGATTAcc                                                                                                                  <  1:17843/140‑1 (MQ=255)                         cTTGTTACAGAGATTGCATCCTGCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTTTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAAGAAAGCTATGCTGAAAAGG‑AAAAAGTAAAACCGATTGCCCTTCg                                                                                                             <  1:110635/140‑1 (MQ=255)                          ttGTTACAGAGATTGCATCCTGCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTTTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGG‑AAAAAGTAAAACCGATTACCCTTCGt                                                                                                            >  2:41372/1‑140 (MQ=255)                           tGTTACAGAGAGTGCATCCTGCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTTTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGG‑AAAAAGTAAAACCGATTACCCTTCGTg                                                                                                           >  2:22636/1‑140 (MQ=255)                             ttACAGAGATTGCATCCTGCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTTTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGG‑AAAAAGTAAAACCGATTACCCTTCGTGAt                                                                                                         >  2:361450/1‑140 (MQ=255)                             ttACAGAGATTGCATCCTGCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTTTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGG‑AAAAAGTAAAACCGATTACCCTTCGTGAt                                                                                                         <  2:347647/140‑1 (MQ=255)                               aCAGAGATTGCATCCTGCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTTTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGG‑AAAAAGTAAAACCGATTACCCTTCGTGATGt                                                                                                       <  1:665368/140‑1 (MQ=255)                                    gaTTGCATCCTGCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTTTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGG‑AAAAAGTAAAACCGATTACCCTTCGTGAt                                                                                                         >  2:595250/1‑133 (MQ=255)                                    gaTTGCATCCTGCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTTTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGG‑AAAAAGTAAAACCGATTACCCTTCGTGAt                                                                                                         <  1:595250/133‑1 (MQ=255)                                     aTTGCATCCTGCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTTTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGG‑AAAAAGTAAAACCGATTACCCTTCGTGATGTCACCat                                                                                                 >  1:119194/1‑140 (MQ=255)                                       tGCATCCTGCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTTTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGG‑AAAAAGTAAAACCGATTACCCTTCGTGATGTCAc                                                                                                    >  2:113974/1‑135 (MQ=255)                                       tGCATCCTGCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTTTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGG‑AAAAAGTAAAACCGATTACCCTTCGTGATGTCAc                                                                                                    <  1:113974/135‑1 (MQ=255)                                       tGCATCCTGCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTTTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGG‑AAAAAGTAAAACCGATTACCCTTCGTGATGTCACCatta                                                                                               >  2:409898/1‑140 (MQ=255)                                       tGCATCCTGCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTTTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGG‑AAAAAGTAAAACCGATTACCCTTCGTGATGTCACCatta                                                                                               >  2:803432/1‑140 (MQ=255)                                       tGCATCCTGCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTTTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGG‑AAAAAGTAAAACCGATTACCCTTCGTGATGTCACCatta                                                                                               >  2:476592/1‑140 (MQ=255)                                       tGCATCCTGCAATTCCCGCTCCCCTTTTGCGGCCATCGCGCTGATTTTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGG‑AAAAAGTAAAACCGATTACCCTTCGTGATGTCACCatta                                                                                               >  1:311313/1‑140 (MQ=255)                                           tCCTGCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTTTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGG‑AAAAAGTAAAACCGATTACCCTTCGTGATGTCACCATTATtga                                                                                           <  2:821663/140‑1 (MQ=255)                                           tCCTGCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTTTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGG‑AAAAAGTAAAACCGATTACCCTTCGTGATGTCACCATTATtga                                                                                           <  2:280234/140‑1 (MQ=255)                                             cTGCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTTTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGG‑AAAAAGTAAAACCGATTACCCTTCGTGATGTCACCATTATtgatg                                                                                         <  2:119194/140‑1 (MQ=255)                                              tGCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTTTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGG‑AAAAAGTAAAACCGATTACCCTTCGTGATGt                                                                                                       >  1:736687/1‑125 (MQ=255)                                              tGCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTTTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGG‑AAAAAGTAAAACCGATTACCCTTCGTGATGt                                                                                                       <  2:736687/125‑1 (MQ=255)                                                          tCCCCTTTTGCGGCCGTCGCGCTGATTTTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGG‑AAAAAGTAAAACCGATTACCCTTCGTGATGTCACCATTATTGATGACGGTAAACTGCg                                                                            >  2:662883/1‑140 (MQ=255)                                                          tCCCCTTTTGCGGCCGTCGCGCTGATTTTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGG‑AAAAAGTAAAACCGATTACCCTTCGTGATGTCACCATTATTGATGACGGTAAACTGCg                                                                            >  1:473287/1‑140 (MQ=255)                                                                 ttGCGGCCGTCGCGCTGATTTTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGG‑AAAAAGTAAAACCGATTACCCTTCGTGATGTCACCATTATTGATGACGGTAAACTGCGTAAAGcc                                                                     >  2:457354/1‑140 (MQ=255)                                                                   gCGGCCGTCGCGCTGATTTTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGG‑AAAAAGTAAAACCGATTACCCTTCGTGATGTCACCATTATTGATGACGGTAAACTGCGTAAAGCCAt                                                                   <  1:313494/140‑1 (MQ=255)                                                                      gCCGTCGCGCTGATTTTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGG‑AAAAAGTAAAACCGATTACCCTTCGTGATGTCACCATTATTGATGACGGTAAACTGCGTAAAGCCATTAc                                                                >  2:605968/1‑140 (MQ=255)                                                                      gCCGTCGCGCTGATTTTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGG‑AAAAAGTAAAACCGATTACCCTTCGTGATGTCACCATTATTGATGACGGTAAACTGCGTAAAGCCATTAc                                                                <  1:90664/140‑1 (MQ=255)                                                                          tCGCGCTGATTTTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGG‑AAAAAGTAAAACCGATTACCCTTCGTGATGTCACCATTATTGATGACGGTAAACTGCGTAAAGCCATTACCgca                                                            >  2:9671/1‑140 (MQ=255)                                                                           cgcgCTGATTTTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGG‑AAAAAGTAAAACCGATTACCCTTCGTGATGTCACCATTATTGATGACGGTAAACTGCGTAAAGCCATTACCgcag                                                           <  1:333136/140‑1 (MQ=255)                                                                           cgcgCTGATTTTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGG‑AAAAAGTAAAACCGATTACCCTTCGTGATGTCACCATTATTGATGACGGTAAACTGCGTAAAGCCATTACCgcag                                                           <  2:426478/140‑1 (MQ=255)                                                                           cgcgCTGATTTTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGG‑AAAAAGTAAAACCGATTACCCTTCGTGATGTCACCATTATTGATGACGGTAAACTGCGTAAAGCCATTACCgcag                                                           <  2:39872/140‑1 (MQ=255)                                                                           cgcgCTGATTTTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGG‑AAAAAGTAAAACCGATTACCCTTCGTGATGTCACCATTATTGATGACGGTAAACTGCGTAAAGCCATTACCgcag                                                           <  1:753621/140‑1 (MQ=255)                                                                           cgcgCTGATTTTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGG‑AAAAAGTAAAACCGATTACCCTTCGTGATGTCACCATTATTGATGACGGTAAACTGCGTAAAGCCATTACCgcag                                                           >  2:162504/1‑140 (MQ=255)                                                                           cgcgCTGATTTTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGG‑AAAAAGTAAAACCGATTACCCTTCGTGATGTCACCATTATTGAAGACGGTAAACTGCGTAAAGCCATTACCgcag                                                           <  1:361450/140‑1 (MQ=255)                                                                             cgcTGATTTTTCTGGCGTTTGCGGAACTGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGG‑AAAAAGTAAAACCGATTACCCTTCGTGATGTCACCATTATTGATGACGGTAAACTGCGTAAAGCCATTACCgcagca                                                         <  1:605968/140‑1 (MQ=255)                                                                             cgcTGATTTTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGG‑AAAAAGTAAAACCGATTACCCTTCGTGATGTCACCATTATTGATGACGGTAAACTGCGTAAAGCCATTACCgcagca                                                         <  1:662883/140‑1 (MQ=255)                                                                                tGATTTTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTTAAAAGG‑AAAAAGTAAAACCGATTACCCTTCGTGATGTCACCATTATTGATGACGGTAAACTGCGTAAAGCCATTACCGCAGCATCa                                                      <  2:752699/140‑1 (MQ=255)                                                                                tGATTTTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGG‑AAAAAGTAAAACCGATTACCCTTCGTGATGTCACCATTATTGATGACGGTAAACTGCGTAAAGCCATTACCGCAGCATCa                                                      <  1:22636/140‑1 (MQ=255)                                                                                tGATTTTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGG‑AAAAAGTAAAACCGATTACCCTTCGTGATGTCACCATTATTGATGACGGTAAACTGCGTAAAGCCATTACCGCAGCATCa                                                      <  2:155685/140‑1 (MQ=255)                                                                                  aTTTTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGG‑AAAAAGTAAAACCGATTACCCTTCGTGATGTCACCATTATTGATGACGGTAAACTGCGTAAAGCCATTACCGCAGCATCACt                                                    <  1:9671/140‑1 (MQ=255)                                                                                     tttCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGG‑AAAAAGTAAAACCGATTACCCTTCGTGATGTCACCATTATTGATGACGGTAAATTGCGTAAAGCCATTACCGCAGCATCACTggg                                                 <  2:335923/140‑1 (MQ=255)                                                                                     tttCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGG‑AAAAAGTAAAACCGATTACCCTTCGTGATGTCACCATTATTGATGACGGTAAACTGCGTAAAGCCATTACCGCAGCATCACTggg                                                 <  1:836756/140‑1 (MQ=255)                                                                                     tttCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGG‑AAAAAGTAAAACCGATTACCCTTCGTGATGTCACCATTATTGATGACGGTAAACTGCGTAAAGCCATTACCGCAGCATCACTggg                                                 <  1:180770/140‑1 (MQ=255)                                                                                       tCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGG‑AAAAAGTAAAACCGATTACCCTTCGTGATGTCACCATTATTGATGACGGTAAACTGCGTAAAGCCATTACCGCAGCATCACTGGGTa                                               <  1:409898/140‑1 (MQ=255)                                                                                          ggCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGG‑AAAAAGTAAAACCGATTACCCTTCGTGATGTCACCATTATTGATGACGGTAAACTGCGTAAAGCCATTACCGCAGCATCACTGGGTAATg                                            <  1:41372/140‑1 (MQ=255)                                                                                                          ggCCAACTCTGCGAGGAAAGCTATGCTGAAAAGG‑AAAAAGTAAAACCGATTACCCTTCGTGATGTCACCATTATTGATGACGGTAAACTGCGTAAAGCCATTACCGCAGCATCACTGGGTAATGCAATGGAATGGTTCGa                            >  1:661221/1‑140 (MQ=255)                                                                                                          ggCCAACTCTGCGAGGAAAGCTATGCTGAAAAGG‑AAAAAGTAAAACCGATTACCCTTCGTGATGTCACCATTATTGATGACGGTAAACTGCGTAAAGCCATTACCGCAGCATCACTGGGTAATGCAATGGAATGGTTCGa                            <  1:162504/140‑1 (MQ=255)                                                                                                            ccAACTCTGCGAGGAAAGCTATGCTGAAAAGG‑AAAAAGTAAAACCGATTACCCTTCGTGATGTCACCATTATTGATGACGGTAAACTGCGTAAAGCCATTACCGCAGCATCACTGGGTAATGCaatggaatgg                                 >  1:861836/1‑133 (MQ=255)                                                                                                            ccAACTCTGCGAGGAAAGCTATGCTGAAAAGG‑AAAAAGTAAAACCGATTACCCTTCGTGATGTCACCATTATTGATGACGGTAAACTGCGTAAAGCCATTACCGCAGCATCACTGGGTAATGCaatggaatgg                                 <  2:861836/133‑1 (MQ=255)                                                                                                              aaCTCTGCGAGGAAAGCTATGCTGAAAAGG‑AAAAAGTAAAACCGATTACCCTTCGTGATGTCACCATTATTGATGACGGTAAACTGCGTAAAGCCATTACCGCAGCATCACTGGGTAATGCAATGGAATGGTTCGAtttt                        <  1:457354/140‑1 (MQ=255)                                                                                                                          aaaGCTATGCTGAAAAGG‑AAAAAGTAAAACCGATTACCCTTCGTGATGTCACCATTATTGATGACGGTAAACTGCGTAAAGCCATTACCGCAGCATCACTGGGTAATGCAATGGAATGGTTCGATTTTGGTGTTTATGGt            >  1:333672/1‑140 (MQ=255)                                                                                                                              cTATGCTGAAAAGG‑AAAAAGTAAAACCGATTACCCTTCGTGATGTCACCATTATTGATGACGGTAAACTGCGTAAAGCCATTACCGCAGCATCACTGGGTAATGCAATGGAATGGTTCGATTTTGGTGTTTATGGTTttg        <  2:333672/140‑1 (MQ=255)                                                                                                                                    tGAAAAGG‑AAAAAGTAAAACCGATTACCCTTCGTGATGTCACCATTATTGATGACGGTAAACTGCGTAAAGCCATTACCGCAGCATCACTGGGTAATGCAATGGAATGGTTCGATTTTGGTGTTTATGGTTTTGTTGCtt  <  2:661221/140‑1 (MQ=255)                                                                                                                                            |                                                                                                                                     GAGGGTGTAAGCAAACCCGCTACGCTTGTTACAGAGATTGCATCCTGCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTTTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGGAAAAAAGTAAAACCGATTACCCTTCGTGATGTCACCATTATTGATGACGGTAAACTGCGTAAAGCCATTACCGCAGCATCACTGGGTAATGCAATGGAATGGTTCGATTTTGGTGTTTATGGTTTTGTTGCTT  >  NC_000913/4330375‑4330646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑