Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,556,769	(T)9→8	coding (116/144 nt)	ytiD →	protein YtiD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,556,761	0	T	.	100.0%	284.0 / NA	66	coding (108/144 nt)	ytiD	protein YtiD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (32/34);  total (32/34)

TTTACAAACTAATTGTTTCAAATAATACATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAA  >  NC_000913/4556626‑4556893                                                                                                                                        |                                                                                                                                     tttACAAACTAATTGTTTCAAATAATACATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGttttt                                                                                                                                  >  1:164641/1‑140 (MQ=255)   tACAAACTAATTGTTTCAAATAATACATGGCTGATTATGCGGAGATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGttttttt                                                                                                                                >  2:770718/1‑140 (MQ=255)   tACAAACTAATTGTTTCAAATAATACATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGttttttt                                                                                                                                <  1:120025/140‑1 (MQ=255)   tACAAACTAATTGTTTCAAATAATACATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGttttttt                                                                                                                                >  1:234776/1‑140 (MQ=255)           aaTTGTTTCAAATAATACATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTtg                                                                                                                       >  2:43075/1‑140 (MQ=255)              tGTTTCAAATAATACATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTtgatg                                                                                                                    <  2:404745/140‑1 (MQ=255)                  tCAAATAATACATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGAttt                                                                                                                <  1:693845/140‑1 (MQ=255)                    aaaTAATACATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGAttttt                                                                                                              >  2:343455/1‑140 (MQ=255)                          taCATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCaaa                                                                                                        >  2:593316/1‑140 (MQ=255)                          taCATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCaaa                                                                                                        >  1:617919/1‑140 (MQ=255)                               ggCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATg                                                                                                   >  1:363279/1‑140 (MQ=255)                                 cTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGAc                                                                                                 >  2:102985/1‑140 (MQ=255)                                    aTTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACttt                                                                                              >  2:206105/1‑140 (MQ=255)                                             aaataaatAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGaa                                                                                     <  1:593316/140‑1 (MQ=255)                                             aaataaatAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGaa                                                                                     <  1:206105/140‑1 (MQ=255)                                              aataaataATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGaaa                                                                                    >  2:485663/1‑140 (MQ=255)                                                  aataatTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTc                                                                                <  1:397197/140‑1 (MQ=255)                                                  aataatTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTc                                                                                <  1:325129/140‑1 (MQ=255)                                                     aatTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGt                                                                             <  2:363279/140‑1 (MQ=255)                                                       tttCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAg                                                                           >  1:133601/1‑140 (MQ=255)                                                         tCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATAATCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGat                                                                         >  2:772602/1‑140 (MQ=255)                                                            cACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATAtt                                                                      >  1:425772/1‑140 (MQ=255)                                                              ccGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCACGACTTTTTTCCTGAAAAGTCAGTAGa                                                                          <  1:830247/134‑1 (MQ=255)                                                              ccGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCACGACTTTTTTCCTGAAAAGTCAGTAGa                                                                          >  2:830247/1‑134 (MQ=255)                                                                ggAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATa                                                                  <  2:204608/140‑1 (MQ=255)                                                                 gAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTc                                                                                <  1:375891/125‑1 (MQ=255)                                                                 gAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTc                                                                                >  2:375891/1‑125 (MQ=255)                                                                        gTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTa                                                          <  1:770718/140‑1 (MQ=255)                                                                        gTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTa                                                          <  1:291104/140‑1 (MQ=255)                                                                        gTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTa                                                          <  1:43075/140‑1 (MQ=255)                                                                                  tGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTtata                                                <  2:241482/140‑1 (MQ=255)                                                                                  tGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACCAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTtata                                                <  2:597844/140‑1 (MQ=255)                                                                                    aCAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACt                                              <  1:772602/140‑1 (MQ=255)                                                                                        gTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGc                                          <  1:498022/140‑1 (MQ=255)                                                                                           ttttCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGa                                       <  1:832482/140‑1 (MQ=255)                                                                                           ttttCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGa                                       >  1:68667/1‑140 (MQ=255)                                                                                           ttttCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGa                                       >  1:299353/1‑140 (MQ=255)                                                                                           ttttCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGa                                       <  2:648864/140‑1 (MQ=255)                                                                                             ttCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACa                                     <  2:166565/140‑1 (MQ=255)                                                                                                   tACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTc                               >  2:499574/1‑140 (MQ=255)                                                                                                     cGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAg                             >  2:312144/1‑140 (MQ=255)                                                                                                       tAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGc                           <  1:102985/140‑1 (MQ=255)                                                                                                       tAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGc                           <  2:164641/140‑1 (MQ=255)                                                                                                       tAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGc                           <  2:617919/140‑1 (MQ=255)                                                                                                              tCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGtctc                    <  2:611683/140‑1 (MQ=255)                                                                                                               cGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGc                           <  1:492328/132‑1 (MQ=255)                                                                                                               cGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGc                           >  2:492328/1‑132 (MQ=255)                                                                                                               cGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCa                   >  2:164799/1‑140 (MQ=255)                                                                                                               cGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCa                   >  2:555976/1‑140 (MQ=255)                                                                                                                ggAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAg                  <  2:139965/140‑1 (MQ=255)                                                                                                                 gAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGa                 <  2:234776/140‑1 (MQ=255)                                                                                                                 gAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGa                 <  2:104199/140‑1 (MQ=255)                                                                                                                 gAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGa                 <  2:753394/140‑1 (MQ=255)                                                                                                                       tCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGa                 <  2:100546/134‑1 (MQ=255)                                                                                                                       tCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGa                 >  1:100546/1‑134 (MQ=255)                                                                                                                       tCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACa           <  2:53858/140‑1 (MQ=255)                                                                                                                       tCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACa           >  2:861031/1‑140 (MQ=255)                                                                                                                       tCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACa           >  2:422008/1‑140 (MQ=255)                                                                                                                       tCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACa           <  1:531521/140‑1 (MQ=255)                                                                                                                       tCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACa           <  1:312144/140‑1 (MQ=255)                                                                                                                            atatACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGAt      <  2:408737/140‑1 (MQ=255)                                                                                                                            atatACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGAt      >  1:853547/1‑140 (MQ=255)                                                                                                                            atatACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGAt      >  1:408737/1‑140 (MQ=255)                                                                                                                                aCTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTTACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTaa  >  1:390270/1‑140 (MQ=255)                                                                                                                                aCTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTaa  >  2:271138/1‑140 (MQ=255)                                                                                                                                aCTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTaa  >  2:252691/1‑140 (MQ=255)                                                                                                                                aCTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTaa  >  2:132884/1‑140 (MQ=255)                                                                                                                                aCTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTaa  >  1:613496/1‑140 (MQ=255)                                                                                                                                aCTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTaa  >  1:56526/1‑140 (MQ=255)                                                                                                                                aCTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTaa  >  2:755930/1‑140 (MQ=255)                                                                                                                                aCTAGCGG‑TTTTTTTTGAGATTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTaa  >  2:453759/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                     TTTACAAACTAATTGTTTCAAATAATACATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAA  >  NC_000913/4556626‑4556893

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑