Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,977,215	(A)8→7	intergenic (‑76/+51)	motA ← / ← flhC	motility protein A/DNA‑binding transcriptional dual regulator FlhC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,977,208	0	A	.	100.0%	218.0 / NA	51	intergenic (‑69/+58)	motA/flhC	motility protein A/DNA‑binding transcriptional dual regulator FlhC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (24/27);  total (24/27)

CACCGGTCATCAAATAACCGCCGAAAACTGTACCGAGAACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTTGTGGGATAATATCGGCAGGATTCTGGGAAAGTTTACGTCTTTT  >  NC_000913/1977073‑1977343                                                                                                                                        |                                                                                                                                        cACCGGTCATCAAATAACCGCCGAAAACTGTACCGAGAACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCaaaaa                                                                                                                                     <  1:771258/140‑1 (MQ=255)       tcatcaAATAACCGCCGAAAACTGTACCGAGAACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCaaaaaaagcag                                                                                                                               >  1:67920/1‑136 (MQ=255)       tcaccaAATAACCGCCGAAAACTGTACCGAGAACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCaaaaaaagcag                                                                                                                               <  1:491966/140‑5 (MQ=255)           caAATAACCGCCGAAAACTGTACCGAGAACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGC‑AAAAAAAGCAGCGGt                                                                                                                          >  1:138961/1‑140 (MQ=255)                     ccgAAAACTGTACCGAGAACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGC‑AAAAAAAGCAGCGGTACGTCGTTAc                                                                                                                <  2:337396/140‑1 (MQ=255)                       gAAAACTGTACCGAGAACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGC‑AAAAAAAGCAGCGGTACGTCGTTACCg                                                                                                              <  1:502319/140‑1 (MQ=255)                          aaCTGTACCGAGAACAACCAGGTAACCTAATAAGATAAGCACAACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGC‑AAAAAAAGCAGCGGTACGTCGTTACCgctg                                                                                                           <  1:285157/140‑1 (MQ=255)                             tGTACCGAGAACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGC‑AAAAAAAGCAGCGGTACGTCGTTACCGctgctg                                                                                                        >  1:572022/1‑140 (MQ=255)                               tACCGAGAACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGC‑AAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGa                                                                                                      >  1:842402/1‑140 (MQ=255)                                    agaACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGC‑AAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGtt                                                                                                 >  2:628708/1‑140 (MQ=255)                                       acaacCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGC‑AAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTgcg                                                                                              >  2:99247/1‑140 (MQ=255)                                        caacCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGC‑AAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTgcgc                                                                                             <  1:400223/140‑1 (MQ=255)                                         aacCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGC‑AAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGcc                                                                                            >  2:374735/1‑140 (MQ=255)                                          acCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGC‑AAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCt                                                                                           <  2:265930/140‑1 (MQ=255)                                          acCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGC‑AAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCt                                                                                           <  1:293819/140‑1 (MQ=255)                                            cAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGC‑AAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCa                                                                                         <  2:842402/140‑1 (MQ=255)                                                tAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGC‑AAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGt                                                                                     <  1:374735/140‑1 (MQ=255)                                                   ccTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGC‑AAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATc                                                                                  >  2:766847/1‑140 (MQ=255)                                                   ccTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGC‑AAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATc                                                                                  >  2:409391/1‑140 (MQ=255)                                                          agataagCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGC‑AAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTaaa                                                                           <  2:67920/140‑1 (MQ=255)                                                              aagCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGC‑AAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGc                                                                       <  1:409391/140‑1 (MQ=255)                                                                 cACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGC‑AAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGc                                                                       >  1:377125/1‑137 (MQ=255)                                                                 cACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGC‑AAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGc                                                                       <  2:377125/137‑1 (MQ=255)                                                                   cGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGC‑AAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTa                                                                  <  2:707444/140‑1 (MQ=255)                                                                      catcatCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGC‑AAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAg                                                                        >  2:97808/1‑131 (MQ=255)                                                                      catcatCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGC‑AAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAg                                                                        <  1:97808/131‑1 (MQ=255)                                                                      catcatCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGC‑AAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTActc                                                               >  1:212320/1‑140 (MQ=255)                                                                      catcatCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGC‑AAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTActc                                                               >  2:829952/1‑140 (MQ=255)                                                                                ccACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGC‑AAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTAct                                                                >  1:276491/1‑129 (MQ=255)                                                                                ccACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGC‑AAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTAct                                                                <  2:276491/129‑1 (MQ=255)                                                                                ccACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGC‑AAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATc                                                     >  1:4910/1‑140 (MQ=255)                                                                                   cTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGC‑AAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCcag                                                  <  2:138961/140‑1 (MQ=255)                                                                                   cTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGC‑AAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCcag                                                  <  1:284261/140‑1 (MQ=255)                                                                                   cTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGC‑AAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCcag                                                  <  2:696236/140‑1 (MQ=255)                                                                                      ttGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGC‑AAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCcagcag                                               <  1:99247/140‑1 (MQ=255)                                                                                      ttGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGC‑AAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCcagcag                                               >  1:523982/1‑140 (MQ=255)                                                                                      ttGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGC‑AAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCcagcag                                               >  1:42718/1‑140 (MQ=255)                                                                                      ttGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGC‑AAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCcagcag                                               >  2:526352/1‑140 (MQ=255)                                                                                      ttGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGC‑AAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCcagcag                                               >  1:142337/1‑140 (MQ=255)                                                                                      ttGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGAGGC‑AAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCcagcag                                               >  1:394417/1‑140 (MQ=255)                                                                                         aCCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGC‑AAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTtg                                            <  2:572022/140‑1 (MQ=255)                                                                                           cATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGC‑AAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTtgtg                                          >  1:799133/1‑140 (MQ=255)                                                                                                  ggATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGC‑AAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTTGTGGGataat                                   <  2:394417/140‑1 (MQ=255)                                                                                                   gATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGC‑AAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTTGTGGGataata                                  >  1:656727/1‑140 (MQ=255)                                                                                                       ttCAGTCGTCAGGCGTTAACGCGCGATTGGGGC‑AAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTTGTGGGATAATATCgg                              >  2:716980/1‑140 (MQ=255)                                                                                                        tCAGTCGTCAGGCGTTAACGCGCGATTGGGGC‑AAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTTGTGGGATAATATCGGc                             >  1:546523/1‑140 (MQ=255)                                                                                                        tCAGTCGTCAGGCGTTAACGCGCGATTGGGGC‑AAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTTGTGGGATAATATCGGc                             <  2:799133/140‑1 (MQ=255)                                                                                                         cAGTCGTCAGGCGTTAACGCGCGATTGGGGC‑AAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTTGTGGGATAATATCGGCa                            <  1:700552/140‑1 (MQ=255)                                                                                                                    cGTTAACGCGCGATTGGGGC‑AAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTTGTGGGATAATATCGGCAGGATTCTGGGa                 <  2:142337/140‑1 (MQ=255)                                                                                                                          cgcgcgATTGGGGC‑AAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTTGTGGGATAATATCGGCAGGATTc                      >  2:599377/1‑129 (MQ=255)                                                                                                                          cgcgcgATTGGGGC‑AAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTTGTGGGATAATATCGGCAGGATTc                      <  1:599377/129‑1 (MQ=255)                                                                                                                           gcgcgATTGGGGC‑AAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTTGTGGGATAATATCGGCAGGATTCTGGGAAAGTTTa          <  1:766847/140‑1 (MQ=255)                                                                                                                             gcgATTGGGGC‑AAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTTGTGGGATAATATCGGCAGGATTCTGGGAAAGTTTACg        <  1:253916/140‑1 (MQ=255)                                                                                                                                   ggggC‑AAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTTGTGGGATAATATCGGCAGGATTCTGGGAAAGTTTACGTCtttt  <  2:42718/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        CACCGGTCATCAAATAACCGCCGAAAACTGTACCGAGAACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTTGTGGGATAATATCGGCAGGATTCTGGGAAAGTTTACGTCTTTT  >  NC_000913/1977073‑1977343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑