Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,985,665	(A)7→6	intergenic (‑73/‑182)	yqeF ← / → yqeG	putative acyltransferase/putative transporter YqeG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,985,659	0	A	.	100.0%	250.4 / NA	58	intergenic (‑67/‑188)	yqeF/yqeG	putative acyltransferase/putative transporter YqeG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (28/30);  total (28/30)

CTAACGCACCACGAAAGCAGCCGATAGGTGTCCGTAACGCCCCGACAATCACAACGTCTTTCATCACAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACC  >  NC_000913/2985529‑2985789                                                                                                                                   |                                                                                                                                   cTAACGCACCACGAAAGCAGCCGATAGGTGTCCGTAACGCCCCGACAATCACAACGTCTTTCATCACAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTaaaaaagtga                                                                                                                           >  2:437074/1‑136 (MQ=255)       caccacGAAAGCAGCCGATAGGTGTCCGTAACGCCCCGACAATCACAACGTCTTTCATCACAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTT‑AAAAAAGTGATTTTTa                                                                                                                    >  1:571220/1‑140 (MQ=255)        accacGAAAGCAGCCGATAGGTGTCCGTAACGCCCCGACAATCACAACGTCTTTCATCACAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTT‑AAAAAAGTGATTTTTAt                                                                                                                   >  2:707234/1‑140 (MQ=255)          cacGAAAGCAGCCGATAGGTGTCCGTAACGCCCCGACAATCACAACGTCTTTCATCACAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTT‑AAAAAAGTGATTTTTATca                                                                                                                 <  1:39884/140‑1 (MQ=255)              aaaGCAGCCGATAGGTGTCCGTAACGCCCCGACAATCACAACGTCTTTCATCACAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTT‑AAAAAAGTGATTTTTATCACaaa                                                                                                             <  1:127452/140‑1 (MQ=255)                  cagcCGATAGGTGTCCGTAACGCCCCGACAATCACAACGTCTTTCATCACAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTT‑AAAAAAGTGATTTTTATCACAAAgg                                                                                                           >  1:9791/1‑138 (MQ=255)                  cagcCGATAGGTGTCCGTAACGCCCCGACAATCACAACGTCTTTCATCACAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTT‑AAAAAAGTGATTTTTATCACAAAgg                                                                                                           <  2:9791/138‑1 (MQ=255)                  cagcCGATAGGTGTCCGTAACGCCCCGACAATCACAACGTCTTTCATCACAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTT‑AAAAAAGTGATTTTTATCACAAAGGaa                                                                                                         >  2:574767/1‑140 (MQ=255)                     ccGATAGGTGTCCGTAACGCCCCGACAATCACAACGTCTTTCATCACAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTT‑AAAAAAGTGATTTTTATCACAAAGGAAata                                                                                                      <  1:707234/140‑1 (MQ=255)                          aGGTGTCCGTAACGCCCCGACAATCACAACGTCTTTCATCACAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTT‑AAAAAAGTGATTTTTATCACAAAGGAAATATGCCt                                                                                                 <  2:264884/140‑1 (MQ=255)                             tgtCCGTAACGCCCCGACAATCACAACGTCTTTCATCACAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTT‑AAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGag                                                                                              <  2:571220/140‑1 (MQ=255)                               tCCGTAACGCCCCGACAATCACAACGTCTTTCATCACAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTT‑AAAAAAGTGATTTTTATCACaaa                                                                                                             >  1:712113/1‑123 (MQ=255)                               tCCGTAACGCCCCGACAATCACAACGTCTTTCATCACAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTT‑AAAAAAGTGATTTTTATCACaaa                                                                                                             <  2:712113/123‑1 (MQ=255)                                ccGTAACGCCCCGACAATCACAACGTCTTTCATCACAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTT‑AAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGagcag                                                                                           <  2:113575/140‑1 (MQ=255)                                ccGTAACGCCCCGACAATCACAACGTCTTTCATCACAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTT‑AAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGagcag                                                                                           <  1:71077/140‑1 (MQ=255)                                    aaCGCCCCGACAATCACAACGTCTTTCATCACAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTT‑AAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGt                                                                                       <  1:170871/140‑1 (MQ=255)                                       gCCCCGACAATCACAACGTCTTTCATCACAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTT‑AAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCag                                                                                    >  2:120637/1‑140 (MQ=255)                                        ccccGACAATCACAACGTCTTTCATCACAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTT‑AAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCaga                                                                                   <  1:574767/140‑1 (MQ=255)                                            gACAATCACAACGTCTTTCATCACAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTT‑AAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGagcag                                                                                           >  1:143645/1‑128 (MQ=255)                                            gACAATCACAACGTCTTTCATCACAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTT‑AAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGagcag                                                                                           <  2:143645/128‑1 (MQ=255)                                                 tCACAACGTCTTTCATCACAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTT‑AAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACt                                                                          <  1:47761/140‑1 (MQ=255)                                                       cGTCTTTCATCACAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTT‑AAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACg                                                                    >  2:507668/1‑140 (MQ=255)                                                        gTCTTTCATCACAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTT‑AAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGt                                                                   <  1:507668/140‑1 (MQ=255)                                                          cTTTCATCACAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTT‑AAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTaa                                                                 <  2:708087/140‑1 (MQ=255)                                                             tcatcaCAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTT‑AAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGt                                                              >  2:229401/1‑140 (MQ=255)                                                                 cacaACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTT‑AAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCGGAGACATAACTGGCACGTAAGGTTTGc                                                          <  2:176202/140‑1 (MQ=255)                                                                 cacaACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTT‑AAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGc                                                          <  1:518353/140‑1 (MQ=255)                                                                   caACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTT‑AAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCaa                                                        >  1:786589/1‑140 (MQ=255)                                                                    aaCCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTT‑AAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTGACAAc                                                       >  2:765053/1‑140 (MQ=255)                                                                        tCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTT‑AAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACt                                                   <  2:819264/140‑1 (MQ=255)                                                                           cACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTT‑AAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAAc                                                >  2:308044/1‑140 (MQ=255)                                                                                aTTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTT‑AAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACccacc                                           >  1:55938/1‑140 (MQ=255)                                                                                aTTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTT‑AAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACccacc                                           <  2:369392/140‑1 (MQ=255)                                                                                aTTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTT‑AAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACccacc                                           >  1:369392/1‑140 (MQ=255)                                                                                   aaCAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTT‑AAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAAt                                        <  1:120637/140‑1 (MQ=255)                                                                                    aCAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTT‑AAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATa                                       <  1:229401/140‑1 (MQ=255)                                                                                    aCAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTT‑AAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATa                                       <  1:189322/140‑1 (MQ=255)                                                                                    aCAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTT‑AAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATa                                       <  2:55938/140‑1 (MQ=255)                                                                                        tGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTT‑AAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTaa                                                 <  2:610981/126‑1 (MQ=255)                                                                                        tGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTT‑AAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTaa                                                 >  1:610981/1‑126 (MQ=255)                                                                                        tGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTT‑AAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAgg                                   >  2:226280/1‑140 (MQ=255)                                                                                         gCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTT‑AAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAggg                                  <  1:297870/140‑1 (MQ=255)                                                                                          cAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTT‑AAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAgggg                                 >  1:120376/1‑140 (MQ=255)                                                                                                  aaTGATTGTTAATTACAGTTATCTTAATTGTTT‑AAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAgg                         >  1:554598/1‑140 (MQ=255)                                                                                                    tGATTGTTAATTACAGTTATCTTAATTGTTT‑AAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCGGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATa                           >  1:413661/1‑136 (MQ=255)                                                                                                    tGATTGTTAATTACAGTTATCTTAATTGTTT‑AAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCGGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATa                           <  2:413661/136‑1 (MQ=255)                                                                                                    tGATTGTTAATTACAGTTATCTTAATTGTTT‑AAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGc                       >  1:728189/1‑140 (MQ=255)                                                                                                          ttAATTACAGTTATCTTAATTGTTT‑AAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAAt                 >  2:232239/1‑140 (MQ=255)                                                                                                              ttACAGTTATCTTAATTGTTT‑AAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTcc             <  2:554598/140‑1 (MQ=255)                                                                                                              ttACAGTTATCTTAATTGTTT‑AAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTcc             <  1:789502/140‑1 (MQ=255)                                                                                                                aCAGTTATCTTAATTGTTT‑AAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCa           >  2:734908/1‑140 (MQ=255)                                                                                                                    ttATCTTAATTGTTT‑AAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACt       >  2:516813/1‑140 (MQ=255)                                                                                                                      aTCTTAATTGTTT‑AAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTa     >  1:734198/1‑140 (MQ=255)                                                                                                                      aTCTTAATTGTTT‑AAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTa     >  2:347742/1‑140 (MQ=255)                                                                                                                         ttAATTGTTT‑AAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAAt                 <  2:313937/125‑1 (MQ=255)                                                                                                                         ttAATTGTTT‑AAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAAt                 >  1:313937/1‑125 (MQ=255)                                                                                                                         ttAATTGTTT‑AAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAAcc  >  1:647633/1‑140 (MQ=255)                                                                                                                              tGTTT‑AAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAAc   >  2:401538/1‑134 (MQ=255)                                                                                                                              tGTTT‑AAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAAc   <  1:401538/134‑1 (MQ=255)                                                                                                                                   |                                                                                                                                   CTAACGCACCACGAAAGCAGCCGATAGGTGTCCGTAACGCCCCGACAATCACAACGTCTTTCATCACAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACC  >  NC_000913/2985529‑2985789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑