Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,988,106	(C)6→7	intergenic (‑147/‑116)	azuC ← / → yecR	uncharacterized protein AzuC/lipoprotein YecR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,988,100	1	.	C	100.0%	122.6 / NA	34	intergenic (‑141/‑122)	azuC/yecR	uncharacterized protein AzuC/lipoprotein YecR
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (21/13);  total (21/13)

CGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAG‑CCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCGCAGCACTGTAACGCGTAAAATAGTGCTTTCTCTTACTCTTCTGGCTGGACCATGA  >  NC_000913/1987962‑1988225                                                                                                                                            |                                                                                                                              cgGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAG‑c                                                                                                                              <  1:750395/140‑1 (MQ=255)        ccGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAG‑ccccccct                                                                                                                       <  2:580861/140‑3 (MQ=255)           aCCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAG‑ccccccctatt                                                                                                                    >  1:692968/1‑135 (MQ=255)              aTTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCCTATTTCt                                                                                                                  <  1:476276/140‑1 (MQ=255)                      taCAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCCTATTTCTGACACCTa                                                                                                          >  1:679428/1‑140 (MQ=255)                        cAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCCTATTTCTGACACCTACa                                                                                                        <  2:692968/140‑1 (MQ=255)                           gtgtTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCCTAttt                                                                                                                    <  1:227269/125‑1 (MQ=255)                           gtgtTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCCTAttt                                                                                                                    >  2:227269/1‑125 (MQ=255)                           gtgtTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCCTATTTCTGACACCTACAGAt                                                                                                     >  1:499875/1‑140 (MQ=255)                           gtgtTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCCTATTTCTGACACCTACAGAt                                                                                                     >  1:36017/1‑140 (MQ=255)                           gtgtTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCCTATTTCTGACACCTACAGAt                                                                                                     >  2:686250/1‑140 (MQ=255)                           gtgtTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCCTATTTCTGACACCTACAGAt                                                                                                     >  2:374279/1‑140 (MQ=255)                             gtTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCCTATTTCTGACACCTACAGATgg                                                                                                   >  2:463720/1‑140 (MQ=255)                             gtTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCCTATTTCTGACACCTACAGATgg                                                                                                   >  2:283383/1‑140 (MQ=255)                             gtTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCCTATTTCTGACACCTACAGATgg                                                                                                   >  2:38304/1‑140 (MQ=255)                                     cTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCCTATTTCTGACACCTACAGATGGCAAGAAAt                                                                                           >  2:538112/1‑140 (MQ=255)                                         gcgcTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAgcg                                                                                       <  2:535613/140‑1 (MQ=255)                                             ttATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTg                                                                                   <  1:677012/140‑1 (MQ=255)                                                       aCAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCt                                                                         >  1:457956/1‑140 (MQ=255)                                                             aTCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCg                                                                   >  2:53219/1‑140 (MQ=255)                                                              tCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCgg                                                                  >  2:46000/1‑140 (MQ=255)                                                                       tcaatcTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCg                                                         <  1:23118/140‑1 (MQ=255)                                                                       tcaatcTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCg                                                         <  2:111227/140‑1 (MQ=255)                                                                       tcaatcTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCg                                                         <  1:74599/140‑1 (MQ=255)                                                                                         aaaTCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCGCAGCACTGTAACGCGTaa                                       >  1:490365/1‑140 (MQ=255)                                                                                           aTCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCCTATTTCTGACACCTACAGCTGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCGCAGCACTGTAACGCGTaaaa                                     >  1:308214/1‑140 (MQ=255)                                                                                                  aaaTACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCGCAGCACTGTAACGCGTAAAATAGTGCt                              <  2:490365/140‑1 (MQ=255)                                                                                                  aaaTACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCGCAGCACTGTAACGCGTAAAATAGTGCt                              <  1:283383/140‑1 (MQ=255)                                                                                                       ccccTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCCTATTTCTTACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCGCAGCACTGTAACGCGTAAAATAGTGCTTtctc                         >  2:251684/1‑140 (MQ=255)                                                                                                       ccccTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCGCAGCACTGTAACGCGTAAAATAGTGCTTtctc                         >  2:608875/1‑140 (MQ=255)                                                                                                          cTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCGCAGCACTGTAACGCGTAAAATAGTGCTTTCTCTTa                      <  2:308214/140‑1 (MQ=255)                                                                                                          cTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCGCAGCACTGTAACGCGTAAAATAGTGCTTTCTCTTa                      >  2:456948/1‑140 (MQ=255)                                                                                                            ttAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCGCAGCACTGTAACGCGTAAAATAGTGCTTTCTCTTAct                    >  2:460592/1‑140 (MQ=255)                                                                                                            ttAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCGCAGCACTGTAACGCGTAAAATAGTGCTTTCTCTTAct                    >  1:95372/1‑140 (MQ=255)                                                                                                                    ataAAAATGATAATCAAAAAACAGCCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCGCAGCACTGTAACGCGTAAAATAGTGCTTTCTCTTACTCTTctggc            <  1:559659/140‑1 (MQ=255)                                                                                                                          aTGATAATCAAAAAACAGCCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCGCAGCACTGTAACGCGTAAAATAGTGCTTTCTCTTACTCTTCTGGCTGGAcc      >  2:524408/1‑140 (MQ=255)                                                                                                                              tAATCAAAAAACAGCCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCGCAGCACTGTAACGCGTAAAATAGTGCTTTCTCTTACTCTTCTGGCTGGACCATga  <  2:36017/140‑1 (MQ=255)                                                                                                                                            |                                                                                                                              CGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAG‑CCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCGCAGCACTGTAACGCGTAAAATAGTGCTTTCTCTTACTCTTCTGGCTGGACCATGA  >  NC_000913/1987962‑1988225

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑