Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,065,747	(C)6→7	coding (18/546 nt)	cobU ←	cobinamide‑P guanylyltransferase/cobinamide kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,065,741	1	.	C	100.0%	206.4 / NA	58	G8G (GGC→GGG)	cobU	cobinamide‑P guanylyltransferase/cobinamide kinase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (29/29);  total (29/29)

CGGCCTTGCCGATGATGTTCTATCCGTGCAGCCATCTCATCATCAAGGATTTGCGAGGTAGCGATATACAGAACCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCG‑CCCCCCGTCACCAAAATCATCATACCGACTCCTGATTCAAATGTATGATGGTATTCATTGAACAGAACAATGGATCCCCCACCGTTGCTGACCGCAAAACATTTACGCGATTAATACTGCGCGTAATATAAT  >  NC_000913/2065613‑2065873                                                                                                                                  |                                                                                                                                     cggcCTTGCCGATGATGTTCTATCCGTGCAGCCATCTCATCATCAAGGATTTGCGAGGTAGCGATATACAGAACCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCG‑cccccccgtca                                                                                                                           <  1:46118/140‑6 (MQ=255)   gcCTTGCCGATGATGTTCTATCCGTGCAGCCATCTCATCATCAAGGATTTGCGAGGTAGCGATATACAGAACCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCCGTCAcc                                                                                                                          <  1:319064/140‑1 (MQ=255)     cTTGCCGATGATGTTCTATCCGTGCAGCCATCTCATCATCAAGGATTTGCGAGGTAGCGATATACAGAACCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCCGTCACCaa                                                                                                                        <  2:532723/140‑1 (MQ=255)     cTTGCCGATGATGTTCTATCCGTGCAGCCATCTCATCATCAAGGATTTGCGAGGTAGCGATATACAGAACCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCCGTCACCaa                                                                                                                        <  1:766104/140‑1 (MQ=255)       tGCCGATGATGTTCTATCCGTGCAGCCATCTCATCATCAAGGATTTGCGAGGTAGCGATATACAGAACCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCCGTCACCaaaa                                                                                                                      <  2:73699/140‑1 (MQ=255)                tgTTCTATCCGTGCAGCCATCTCATCATCAAGGATTTGCGAGGTAGCGATATACAGAACCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCCGTCACCAAAATCATCATAc                                                                                                             >  1:32629/1‑140 (MQ=255)                 gTTCTATCCGTGCAGCCATCTCATCATCAAGGATTTGCGAGGTAGCGATATACAGAACCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGTCTCTTCCCGCGCCGTGCGCCCCCCCGTCACCAAAATCATCATGcc                                                                                                            <  2:296759/140‑1 (MQ=255)                 gTTCTATCCGTGCAGCCATCTCATCATCAAGGATTTGCGAGGTAGCGATATACAGAACCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCCGTCACCAAAATCATCATAcc                                                                                                            <  2:139811/140‑1 (MQ=255)                       tCCGTGCAGCCATCTCATCATCAAGGATTTGCGAGGTAGCGATATACAGAACCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCCGTCACCAAAATCATCATACCGACTcc                                                                                                      >  1:170249/1‑140 (MQ=255)                          gTGCAGCCATCTCATCATCAAGGATTTGCGAGGTAGCGATATACAGAACCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCCGTCACCAAAATCATCATACCGACTCCTGa                                                                                                   >  2:86544/1‑140 (MQ=255)                           tGCAGCCATCTCATCATCAAGGATTTGCGAGGTAGCGATATACAGAACCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCCGTCACCAAAATCATCATACCGACTCCTGAt                                                                                                  <  1:53570/140‑1 (MQ=255)                           tGCAGCCATCTCATCATCAAGGATTTGCGAGGTAGCGATATACAGAACCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCCGTCACCAAAATCATCATACCGACTCCTGAt                                                                                                  >  2:277040/1‑140 (MQ=255)                            gCAGCCATCTCATCATCAAGGATTTGCGAGGTAGCGATATACAGAACCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCCGTCACCAAAATCATCATACCGACTCCGGAtt                                                                                                 >  2:795404/1‑140 (MQ=255)                            gCAGCCATCTCATCATCAAGGATTTGCGAGGTAGCGATATACAGAACCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTAGCGACTCTTCCCGCTCCGTGCGCCCCCCCGTCACCAAAATCATCATACCGACTCCTGAtt                                                                                                 >  2:59863/1‑140 (MQ=255)                               gCCATCTCATCATCAAGGATTTGCGAGGTAGCGATATACAGAACCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCCGTCACCAAAATCATCATACCGACTcc                                                                                                      >  1:225841/1‑132 (MQ=255)                               gCCATCTCATCATCAAGGATTTGCGAGGTAGCGATATACAGAACCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCCGTCACCAAAATCATCATACCGACTcc                                                                                                      <  2:225841/132‑1 (MQ=255)                               gCCATCTCATCATCAAGGATTTGCGAGGTAGCGATATACAGAACCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCCGTCACCAAAATCATCATACCGACTCCTGATTCaa                                                                                              <  1:64094/140‑1 (MQ=255)                               gCCATCTCATCATCAAGGATTTGCGAGGTAGCGATATACAGAACCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTAGCGACTCTTCCCGCTCCGTGCGCCCCCCCGTCACCAAAATCATCATACCGACTCCTGATTCaa                                                                                              <  1:59863/140‑1 (MQ=255)                                    ctcATCATCAAGGATTTGCGAGGTAGCGATATACAGAACCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCCGTCACCAAAATCATCATACCGACTCCTGATTCAAATGTa                                                                                         <  2:32629/140‑1 (MQ=255)                                    ctcATCATCAAGGATTTGCGAGGTAGCGATATACAGAACCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCCGTCACCAAAATCATCATACCGACTCCTGATTCAAATGTa                                                                                         <  1:277040/140‑1 (MQ=255)                                    ctcATCATCAAGGATTTGCGAGGTAGCGATATACAGAACCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCCGTCACCAAAATCATCATACCGACTCCTGATTCAAATGTa                                                                                         <  1:795404/140‑1 (MQ=255)                                      catcatcaAGGATTTGCGAGGTAGCGATATACAGAACCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCCGTCACCAAAATCATCATACCGACTCCTGATTCAAATGTatg                                                                                       <  1:86544/140‑1 (MQ=255)                                              aGGATTTGCGAGGTAGCGATATACAGAACCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCCGTCACCAAAATCATCATACCGACTCCTGATTCAAATGTatgatg                                                                                    >  1:379649/1‑135 (MQ=255)                                              aGGATTTGCGAGGTAGCGATATACAGAACCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCCGTCACCAAAATCATCATACCGACTCCTGATTCAAATGTatgatg                                                                                    <  2:379649/135‑1 (MQ=255)                                              aGGATTTGCGAGGTAGCGATATACAGAACCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCCGTCACCAAAATCATCATACCGACTCCTGATTCAAATGTATGATGGTAtt                                                                               >  1:789660/1‑140 (MQ=255)                                              aGGATTTGCGAGGTAGCGATATACAGAACCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCCGTCACCAAAATCATCATACCGACTCCTGATTCAAATGTATGATGGTAtt                                                                               >  2:710824/1‑140 (MQ=255)                                               ggATTTGCGAGGTAGCGATATACAGAACCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCCGTCACCAAAATCATCATACCGACTCCTGATTCAAATGTATGATGGTATTc                                                                              >  1:312195/1‑140 (MQ=255)                                                    tGCGAGGTAGCGATATACAGAACCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCCGTCACCAAAATCATCATACCGACTCCTGATTCAAATGTATGATGGTATTCATTga                                                                         >  1:314450/1‑140 (MQ=255)                                                    tGCGAGGTAGCGATATACAGAACCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCCGTCACCAAAATCATCATACCGACTCCTGATTCAAATGTATGATGGTATTCATTga                                                                         >  1:497398/1‑140 (MQ=255)                                                     gCGAGGTAGCGATATACAGAACCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCCGTCACCAAAATCATCATACCGACTCCTGATTCAAATGTATGATGGTATTCATTgaa                                                                        <  2:789660/140‑1 (MQ=255)                                                     gCGAGGTAGCGATATACAGAACCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCCGTCACCAAAATCATCATACCGACTCCTGATTCAAATGTATGATGGTATTCATTgaa                                                                        <  2:312195/140‑1 (MQ=255)                                                          gTAGCGATATACAGAACCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCCGTCACCAAAATCATCATACCGACTCCTGATTCAAATGTATGATGGTATTCATTgaacagaa                                                                   <  2:170249/140‑1 (MQ=255)                                                                        aaCCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCCGTCACCAAAATCATCATACCGACTCCTGATTCAAATGTATGATGGTATTCATTGAACAGAACAATGGATcccc                                                       <  1:650960/138‑1 (MQ=255)                                                                        aaCCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCCGTCACCAAAATCATCATACCGACTCCTGATTCAAATGTATGATGGTATTCATTGAACAGAACAATGGATcccc                                                       >  2:650960/1‑138 (MQ=255)                                                                        aaCCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCCGTCACCAAAATCATCATACCGACTCCTGATTCAAATGTATGATGGTATTCATTGAACAGAACAATGGATCCCCCa                                                     >  1:183121/1‑140 (MQ=255)                                                                        aaCCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCCGTCACCAAAATCATCATACCGACTCCTGATTCAAATGTATGATGGTATTCATTGAACAGAACAATGGATCCCCCa                                                     >  2:415446/1‑140 (MQ=255)                                                                          ccTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCCGTCACCAAAATCATCATACCGACTCCTGATTCAAATGTATGATGGTATTCATTGAACAGAACAATGGATCCCCCAcc                                                   >  1:50299/1‑140 (MQ=255)                                                                           cTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCCGTCACCAAAATCATCATACCGACTCCTGATTCAAATGTATGATGGTATTCATTGAACAGAACAATGGATCCCCCACCg                                                  >  1:475382/1‑140 (MQ=255)                                                                             gtgAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCCGTCACCAAAATCATCATACCGACTCCTGATTCAAATGTATGATGGTATTCATTGAACAGAACAATgg                                                             <  2:185927/127‑1 (MQ=255)                                                                             gtgAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCCGTCACCAAAATCATCATACCGACTCCTGATTCAAATGTATGATGGTATTCATTGAACAGAACAATgg                                                             >  1:185927/1‑127 (MQ=255)                                                                             gtgAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCCGTCACCAAAATCATCATACCGACTCCTGATTCAAATGTATGATGGTATTCATTGAACAGAACAATGGATCCCCCACCGtt                                                >  1:17401/1‑140 (MQ=255)                                                                             gtgAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCCGTCACCAAAATCATCATACCGACTCCTGATTCAAATGTATGATGGTATTCATTGAACAGAACAATGGATCCCCCACCGtt                                                >  2:549465/1‑140 (MQ=255)                                                                               gAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCCGTCACCAAAATCATCATACCGACTCCTGATTCAAATGTATGATGGTATTCATTGAACAGAACAATGGATcccc                                                       <  2:721534/131‑1 (MQ=255)                                                                               gAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCCGTCACCAAAATCATCATACCGACTCCTGATTCAAATGTATGATGGTATTCATTGAACAGAACAATGGATcccc                                                       >  1:721534/1‑131 (MQ=255)                                                                                     tCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCCGTCACCAAAATCATCATACCGACTCCTGATTCAAATGTATGATGGTATTCATTGAACAGAACAATGGATCCCCCACCGTTGCTGAccc                                        >  2:786375/1‑139 (MQ=255)                                                                                                 gcCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCCGTCACCAAAATCATCATACCGACTCCTGATTCAAATGTATGATGGTATTCATTGAACAGAACAATGGATCCCCCACCGTTGCTGACCGCAAAACATTTAc                            <  1:837484/140‑1 (MQ=255)                                                                                                 gcCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCCGTCACCAAAATCATCATACCGACTCCTGATTCAAATGTATGATGGTATTCATTGAACAGAACAATGGATCCCCCACCGTTGCTGACCGCAAAACATTTAc                            <  2:314450/140‑1 (MQ=255)                                                                                                 gcCTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCCGTCACCAAAATCATCATACCGACTCCTGATTCAAATGTATGATGGTATTCATTGAACAGAACAATGGATCCCCCACCGTTGCTGACCGCAAAACATTTAc                            <  2:497398/140‑1 (MQ=255)                                                                                                  ccTCTGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCCGTCACCAAAATCATCATACCGACTCCTGATTCAAATGTATGATGGTATTCATTGAACAGAACAATGGATCCCCCACCGTTGCTGACCGCAAAACATTTAcg                           >  2:306066/1‑140 (MQ=255)                                                                                                   ctctGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCCGTCACCAAAATCATCATACCGACTCCTGATTCAAATGTATGATGGTATTCATTGAACAGAACAATGGATCCCCCACCGTTGCTGACCGCAAAACATTTAcgc                          >  2:520227/1‑140 (MQ=255)                                                                                                    tctGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCCGTCACCAAAATCATCATACCGACTCCTGATTCAAATGTATGATGGTATTCATTGAACAGAACAATGGATCCCCCACCGTTGCTGACCGCAAAACATTTAc                            >  1:838986/1‑137 (MQ=255)                                                                                                    tctGCGTGGCGACTCTTCCCGCTCCGTGCGCCCCCCCGTCACCAAAATCATCATACCGACTCCTGATTCAAATGTATGATGGTATTCATTGAACAGAACAATGGATCCCCCACCGTTGCTGACCGCAAAACATTTAc                            <  2:838986/137‑1 (MQ=255)                                                                                                           ggCGACTCTTCCCGCTCCGTGCGCCCCCCCGTCACCAAAATCATCATACCGACTCCTGATTCAAATGTATGATGGTATTCATTGAACAGAACAATGGATCCCCCACCGTTGCTGACCGCAAAACATTTACGCGATTAATa                  >  2:134175/1‑140 (MQ=255)                                                                                                            gCGACTCTTCCCGCTCCGTGCGCCCCCCCGTCACCAAAATCATCATACCGACTCCTGATTCAAATGTATGATGGTATTCATTGAACAGAACAATGGATCCCCCACCGTTGCTGACCGCAAAACATTTACGCGATTAATAc                 <  1:306066/140‑1 (MQ=255)                                                                                                             cGACTCTTCCCGCTCCGTGCGCCCCCCCGTCACCAAAATCATCATACCGACTCCTGATTCAAATGTATGATGGTATTCATTGAACAGAACAATGGATCCCCCACCGTTGCTGACCGCAAAACATTTACGCGATTAATACt                <  2:50299/140‑1 (MQ=255)                                                                                                                ctctTCCCGCTCCGTGCGCCCCCCCGTCACCAAAATCATCATACCGACTCCTGATTCAAATGTATGATGGTATTCATTGAACAGAACAATGGATCCCCCACCGTTGCTGACCGCAAAACATTTACGCGATTAATACt                >  1:607628/1‑137 (MQ=255)                                                                                                                ctctTCCCGCTCCGTGCGCCCCCCCGTCACCAAAATCATCATACCGACTCCTGATTCAAATGTATGATGGTATTCATTGAACAGAACAATGGATCCCCCACCGTTGCTGACCGCAAAACATTTACGCGATTAATACt                <  2:607628/137‑1 (MQ=255)                                                                                                                  ctTCCCGCTCCGTGCGCCCCCCCGTCACCAAAATCATCATACCGACTCCTGATTCAAATGTATGATGGTATTCATTGAACAGAACAATGGATCCCCCACCGTTGCTGACCGCAAAACATTTACGCGATTAATACTGcgcg           <  2:385889/140‑1 (MQ=255)                                                                                                                    tCCCGCTCCGTGCGCCCCCCCGTCACCAAAATCATCATACCGACTCCTGATTCAAATGTATGATGGTATTCATTGAACAGAACAATGGATCCCCCACCGTTGCTGACCGCAAAACATTTACGCGATTAATACTGCGCGTa         <  1:855134/140‑1 (MQ=255)                                                                                                                           ccGTGCGCCCCCCCGTCACCAAAATCATCATACCGACTCCTGATTCAAATGTATGATGGTATTCATTGAACAGAACAATGGATCCCCCACCGTTGCTGACCGCAAAACATTTACGCGATTAATACTGCGCGTAATATGAt  >  2:530446/1‑140 (MQ=255)                                                                                                                                  |                                                                                                                                     CGGCCTTGCCGATGATGTTCTATCCGTGCAGCCATCTCATCATCAAGGATTTGCGAGGTAGCGATATACAGAACCTGTGAAGAGTCCCCAATAAGCGCCTCTGCGTGGCGACTCTTCCCGCTCCGTGCG‑CCCCCCGTCACCAAAATCATCATACCGACTCCTGATTCAAATGTATGATGGTATTCATTGAACAGAACAATGGATCCCCCACCGTTGCTGACCGCAAAACATTTACGCGATTAATACTGCGCGTAATATAAT  >  NC_000913/2065613‑2065873

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑