Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,565,289	G→A	S431S (AGC→AGT)	glgP ←	glycogen phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,565,289	0	G	A	100.0%	143.0 / NA	43	S431S (AGC→AGT)	glgP	glycogen phosphorylase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/23);  total (20/23)

AGCCAGCGACGCGGCGTCACACCGTTGGTGACGTTGGTGAAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCATCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTT  >  NC_000913/3565161‑3565417                                                                                                                                 |                                                                                                                                 agccagcGACGCGGCGTCACACCGTTGGTGACGTTGGTGAAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCATCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGACTCACaacaac                                                                                                                       >  2:364912/1‑140 (MQ=255)       cGACGCGGCGTCACACCGTTGGTGACGTTGGTGAAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCATCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGACTCACAACAACCgccag                                                                                                                 >  2:225194/1‑140 (MQ=255)        gACGCGGCGTCACACCGTTGGTGACGTTGGTGAAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCATCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGACTCACAACAACCgccagc                                                                                                                <  1:814690/140‑1 (MQ=255)               cgTCACACCGTTGGTGACGTTGGTGAAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCATCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGACTCACAACAACCGCCAGCCAGGCCa                                                                                                         <  1:749142/140‑1 (MQ=255)                    cacCGTTGGTGACGTTGGTGAAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCATCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGACTCACAACAACCGCCAGCCAGGCCATACGc                                                                                                    >  2:29557/1‑140 (MQ=255)                       cgttggtgacgttggtgaAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCATCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGACTCACAACAACCGCCAGCCAGGCCATACGcaca                                                                                                 >  2:300553/1‑140 (MQ=255)                               acgttggtgaAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCATCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGACTCACAACAACCGCCAGCCAGGCCATACGCACACGACGAcc                                                                                         >  1:579651/1‑140 (MQ=255)                                cgttggtgaAACGCCCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCATCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGACTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCg                                                                                        <  2:51866/140‑1 (MQ=255)                                cgttggtgaAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCATCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGACTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCg                                                                                        <  2:338313/140‑1 (MQ=255)                                    ggtgaAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCATCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGACTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTgg                                                                                    >  2:703870/1‑140 (MQ=255)                                     gtgaAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCATCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGACTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGa                                                                                   <  1:300553/140‑1 (MQ=255)                                     gtgaAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCATCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGACTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGa                                                                                   <  1:604220/140‑1 (MQ=255)                                       gaAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCATCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGACTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGAtt                                                                                 <  1:264522/140‑1 (MQ=255)                                           cGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCATCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGACTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATtcatc                                                                             >  2:321909/1‑140 (MQ=255)                                             aCCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCATCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGACTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCaa                                                                           <  1:364912/140‑1 (MQ=255)                                             aCCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCATCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGACTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCaa                                                                           >  1:90378/1‑140 (MQ=255)                                                      gATTTTCGCAAAGTCGGCAAACAACGATTGCACCATCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGACTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACg                                                                  <  1:29557/140‑1 (MQ=255)                                                        ttttCGCAAAGTCGGCAAACAACGATTGCACCATCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGACTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGcc                                                                <  2:365438/140‑1 (MQ=255)                                                        ttttCGCAAAGTCGGCAAACAACGATTGCACCATCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGACTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGcc                                                                <  1:225194/140‑1 (MQ=255)                                                         tttCGCAAAGTCGGCAAACAACGATTGCACCATCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGACTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGccc                                                               >  1:403419/1‑140 (MQ=255)                                                            cGCAAAGTCGGCAAACAACGATTGCACCATCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGACTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTc                                                            >  2:811708/1‑140 (MQ=255)                                                                aaGTCGGCAAACAACGATTGCACCATCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGACTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCcag                                                        <  1:811708/140‑1 (MQ=255)                                                                aaGTCGGCAAACAACGATTGCACCATCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGACTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCcag                                                        <  2:150478/140‑1 (MQ=255)                                                                aaGTCGGCAAACAACGATTGCACCATCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGACTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCcag                                                        <  2:403419/140‑1 (MQ=255)                                                                 aGTCGGCAAACAACGATTGCACCATCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGACTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCcagc                                                       >  1:563043/1‑140 (MQ=255)                                                                     ggCAAACAACGATTGCACCATCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGACTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGAt                                                   <  1:502696/140‑1 (MQ=255)                                                                           caacGATTGCACCATCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGACTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTAt                                             <  1:703870/140‑1 (MQ=255)                                                                           caacGATTGCACCATCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGACTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTAt                                             <  2:763616/140‑1 (MQ=255)                                                                             acGATTGCACCATCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGACTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCg                                           >  1:308756/1‑140 (MQ=255)                                                                                    cACCATCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGACTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGAt                                    <  2:579651/140‑1 (MQ=255)                                                                                                agagTGCAGTTCCGATACACCGTTAACTTTGTGACTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCa                        <  2:563043/140‑1 (MQ=255)                                                                                                agagTGCAGTTCCGATACACCGTTAACTTTGTGACTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCa                        <  2:90378/140‑1 (MQ=255)                                                                                                  agTGCAGTTCCGATACACCGTTAACTTTGTGACTCACGACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAg                      >  2:27279/1‑140 (MQ=255)                                                                                                  agTGCAGTTCCGATACACCGTTAACTTTGTGACTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAg                      >  1:204080/1‑140 (MQ=255)                                                                                                    tGCAGTTCCGATACACCGTTAACTTTGTGACTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCa                        >  1:798976/1‑136 (MQ=255)                                                                                                    tGCAGTTCCGATACACCGTTAACTTTGTGACTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCa                        <  2:798976/136‑1 (MQ=255)                                                                                                       aGTTCCGATACACCGTTAACTTTGTGACTCACGACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGtttt                 <  1:27279/140‑1 (MQ=255)                                                                                                       aGTTCCGATACACCGTTAACTTTGTGACTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCaa                       <  2:186894/134‑1 (MQ=255)                                                                                                       aGTTCCGATACACCGTTAACTTTGTGACTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCaa                       >  1:186894/1‑134 (MQ=255)                                                                                                         ttCCGATACACCGTTAACTTTGTGACTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCa               >  1:848523/1‑140 (MQ=255)                                                                                                                   ccGTTAACTTTGTGACTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTc     >  2:649500/1‑140 (MQ=255)                                                                                                                    cGTTAACTTTGTGACTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCg    >  1:658294/1‑140 (MQ=255)                                                                                                                      ttAACTTTGTGACTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGtt  <  2:308756/140‑1 (MQ=255)                                                                                                                      ttAACTTTGTGACTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGtt  >  1:608022/1‑140 (MQ=255)                                                                                                                                 |                                                                                                                                 AGCCAGCGACGCGGCGTCACACCGTTGGTGACGTTGGTGAAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCATCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTT  >  NC_000913/3565161‑3565417

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑