Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,857,081	A→G	F442L (TTC→CTC)	yidJ ←	putative sulfatase/phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,857,081	0	A	G	100.0%	344.2 / NA	92	F442L (TTC→CTC)	yidJ	putative sulfatase/phosphatase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (52/40);  total (52/40)

TGTGTCGGTAGGCCGGTGTCATAGTCGCGTACAACTGGCGAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGATCAGGTTATGCATTTCATTTGGGTCATTACGGCGATCGTAAAGTTCATCACTGGTG  >  NC_000913/3856948‑3857217                                                                                                                                      |                                                                                                                                         tgtgTCGGTAGGCCGGTGTCATAGTCGCGTACAACTGGCGAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAGCGGATc                                                                                                                                    >  1:144593/1‑140 (MQ=255)  gtgtCGGTAGGCCGGTGTCATAGTCGCGTACAACTGGCGAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAGCGGATcg                                                                                                                                   >  2:253574/1‑140 (MQ=255)   tgtCGGGAGGCCGGGGTCATAGTCGCGTACAACTGGCGAATAGGCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCGACTGCGGAGCGGATcgc                                                                                                                                  >  2:769707/1‑140 (MQ=255)          aGGCCGGTGTCATAGTCGCGTACAACTGGCGAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAGCGGATCGCGAAtttt                                                                                                                           >  2:746419/1‑140 (MQ=255)            gCCGGTGTCATAGTCGCGTACAACTGGCGAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAGCGGATCGCGAATTTTGt                                                                                                                         >  2:58885/1‑140 (MQ=255)            gCCGGTGTCATAGTCGCGTACAACTGGCGAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAGCGGATCGCGAATTTTGt                                                                                                                         <  2:789687/140‑1 (MQ=255)              cGGTGTCATAGTCGCGTACAACTGGCGAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAGCGGATCGCGAATTTTGTcc                                                                                                                       >  2:63981/1‑140 (MQ=255)               ggTGTCATAGTCGCGTACAACTGGCGAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAGCGGATCGCGAATTTTGTCCa                                                                                                                      <  1:253574/140‑1 (MQ=255)                gtgtCATAGTCGCGTACAACTGGCGAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAGCGGATCGCGAATTTTGTCCAt                                                                                                                     >  2:314321/1‑140 (MQ=255)                gtgtCATAGTCGCGTACAACTGGCGAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAGCGGATCGCGAATTTTGTCCAt                                                                                                                     >  2:673722/1‑140 (MQ=255)                 tgtCATAGTCGCGTACAACTGGCGAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAGCGGATCGCGAATTTTGTCCATg                                                                                                                    <  1:243172/140‑1 (MQ=255)                 tgtCATAGTCGCGTACAACTGGCGAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAGCGGATCGCGAATTTTGTCCATg                                                                                                                    >  1:34999/1‑140 (MQ=255)                 tgtCATAGTCGCGTACAACTGGCGAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAGCGGATCGCGAATTTTGTCCATg                                                                                                                    <  1:746419/140‑1 (MQ=255)                 tgtCATAGTCGCGTACAACTGGCGAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAGCGGATCGCGAATTTTGTCCATg                                                                                                                    <  1:673722/140‑1 (MQ=255)                 tgtCATAGTCGCGTACAACTGGCGAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAGCGGATCGCGAATTTTGTCCATg                                                                                                                    <  1:63981/140‑1 (MQ=255)                 tgtCATAGTCGCGTACAACTGGCGAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAGCGGATCGCGAATTTTGTCCATg                                                                                                                    <  2:514682/140‑1 (MQ=255)                 tgtCATAGTCGCGTACAACTGGCGAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAGCGGATCGCGAATTTTGTCCATg                                                                                                                    <  1:58885/140‑1 (MQ=255)                   tCATAGTCGCGTACAACTGGCGAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAGCGGATCGCGAATTTTGTCCATGTa                                                                                                                  >  2:122303/1‑140 (MQ=255)                     aTAGTCGCGTACAACTGGCGAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAGCGGATCGCGAATTTTGTCCATGTAAt                                                                                                                >  1:185334/1‑140 (MQ=255)                           gcgTACAACTGGCGAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATa                                                                                                          >  2:313585/1‑140 (MQ=255)                             gTACAACTGGCGAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAg                                                                                                        >  2:763168/1‑140 (MQ=255)                             gTACAACTGGCGAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAg                                                                                                        >  1:9207/1‑140 (MQ=255)                              tACAACTGGCGAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAgg                                                                                                       >  1:147665/1‑140 (MQ=255)                                       cGAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAg                                                                                                        <  2:110498/130‑1 (MQ=255)                                       cGAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAg                                                                                                        >  1:110498/1‑130 (MQ=255)                                         aaTAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCAtt                                                                                            >  2:449661/1‑140 (MQ=255)                                           tAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCAtttt                                                                                          >  2:256004/1‑140 (MQ=255)                                                 tCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCg                                                                                    <  2:407854/140‑1 (MQ=255)                                                 tCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCg                                                                                    <  2:185334/140‑1 (MQ=255)                                                 tCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCg                                                                                    <  1:296353/140‑1 (MQ=255)                                                 tCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCg                                                                                    <  2:9207/140‑1 (MQ=255)                                                   ttGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGaa                                                                                  <  2:147665/140‑1 (MQ=255)                                                        gacgtggacgAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCt                                                                             >  2:231024/1‑140 (MQ=255)                                                          cgtggacgAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGc                                                                           <  1:763168/140‑1 (MQ=255)                                                            tggacgAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCaa                                                                         >  2:83199/1‑140 (MQ=255)                                                            tggacgAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCaa                                                                         >  2:782474/1‑140 (MQ=255)                                                             ggacgAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCaaa                                                                        >  2:363984/1‑140 (MQ=255)                                                             ggacgAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCaaa                                                                        <  1:449661/140‑1 (MQ=255)                                                              gacgAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCaaaa                                                                       >  1:241109/1‑140 (MQ=255)                                                               acgAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAAc                                                                      >  1:483784/1‑140 (MQ=255)                                                               acgAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAAc                                                                      >  1:96048/1‑140 (MQ=255)                                                                      gCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATatc                                                               <  2:241109/140‑1 (MQ=255)                                                                       cccccATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATatca                                                              <  2:483784/140‑1 (MQ=255)                                                                       cccccATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATatca                                                              <  2:96048/140‑1 (MQ=255)                                                                           cATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGa                                                          <  1:83199/140‑1 (MQ=255)                                                                            aTCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGAt                                                         <  1:256004/140‑1 (MQ=255)                                                                                 gcgcGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGATCAGGt                                                    >  1:385960/1‑140 (MQ=255)                                                                                  cgcgGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGAt                                                         >  2:513380/1‑134 (MQ=255)                                                                                  cgcgGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGAt                                                         <  1:513380/134‑1 (MQ=255)                                                                                  cgcgGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGATCAGGtt                                                   >  2:70747/1‑140 (MQ=255)                                                                                  cgcgGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGATCAGGtt                                                   >  2:213717/1‑140 (MQ=255)                                                                                  cgcgGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGATCAGGtt                                                   >  2:118533/1‑140 (MQ=255)                                                                                  cgcgGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGATCAGGtt                                                   >  2:157292/1‑140 (MQ=255)                                                                                  cgcgGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGATCAGGtt                                                   >  1:813313/1‑140 (MQ=255)                                                                                  cgcgGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGATCAGGtt                                                   >  1:293815/1‑140 (MQ=255)                                                                                      gCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGa                                                          >  2:540963/1‑129 (MQ=255)                                                                                      gCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGa                                                          <  1:540963/129‑1 (MQ=255)                                                                                      gCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGa                                                          <  2:737333/129‑1 (MQ=255)                                                                                      gCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGa                                                          >  1:737333/1‑129 (MQ=255)                                                                                       ccGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGAt                                                         >  1:63003/1‑129 (MQ=255)                                                                                       ccGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGAt                                                         <  2:63003/129‑1 (MQ=255)                                                                                       ccGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGATCAGGTTATGca                                              >  2:321413/1‑140 (MQ=255)                                                                                                 ttACGCCACGGACGCAGACTCCATTGGTAACTGCGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGATCAGGTTATGCATTTCATTTgg                                    >  1:53988/1‑140 (MQ=255)                                                                                                  tACGCCACGGACGCAGACTCCATTGGTAACTGCGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGATCAGGTTATGCATTTCATTTggg                                   <  1:231024/140‑1 (MQ=255)                                                                                                  tACGCCACGGACGCAGACTCCATTGGTAACTGCGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGATCAGGTTATGCATTTCATTTggg                                   <  1:213717/140‑1 (MQ=255)                                                                                                         cGGACGCAGACTCCATTGGTAACTGCGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGATCAGGTTATGca                                              <  1:49639/122‑1 (MQ=255)                                                                                                         cGGACGCAGACTCCATTGGTAACTGCGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGATCAGGTTATGca                                              >  2:49639/1‑122 (MQ=255)                                                                                                         cGGACGCAGACTCCATTGGTAACTGCGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGATCAGGTTATGCATTTCATTTGGGTCATTAc                            >  1:251126/1‑140 (MQ=255)                                                                                                         cGGACGCAGACTCCATTGGTAACTGCGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGATCAGGTTATGCATTTCATTTGGGTCATTAc                            >  2:861343/1‑140 (MQ=255)                                                                                                           gACGCAGACTCCATTGGTAACTGCGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGATCAGGTTATGCATTTCATTTGGGTCATTACgg                          <  2:34999/140‑1 (MQ=255)                                                                                                           gACGCAGACTCCATTGGTAACTGCGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGATCAGGTTATGCATTTCATTTGGGTCATTACgg                          <  1:797799/140‑1 (MQ=255)                                                                                                           gACGCAGACTCCATTGGTAACTGCGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGATCAGGTTATGCATTTCATTTGGGTCATTACgg                          <  2:251126/140‑1 (MQ=255)                                                                                                              gCAGACTCCATTGGTAACTGCGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGATCAGGTTATGCATTTCATTTGGGTCATTACGGCGa                       >  1:391740/1‑140 (MQ=255)                                                                                                                   cTCCATTGGTAACTGCGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGATCAGGTTATGCATTTCATTTGGGTCATTACGGCGATCGTa                  <  1:861343/140‑1 (MQ=255)                                                                                                                   cTCCATTGGTAACTGCGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGATCAGGTTATGCATTTCATTTGGGTCATTACGGCGATCGTa                  <  2:391740/140‑1 (MQ=255)                                                                                                                      cATTGGTAACTGCGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGATCAGGTTATGCATTTCATTTGGGTCATTACGGCGATCGTa                  >  2:367489/1‑137 (MQ=255)                                                                                                                      cATTGGTAACTGCGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGATCAGGTTATGCATTTCATTTGGGTCATTACGGCGATCGTa                  <  1:367489/137‑1 (MQ=255)                                                                                                                      cATTGGTAACTGCGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGATCAGGTTATGCATTTCATTTGGGTCATTACGGCGATCGTAAAg               >  2:632473/1‑140 (MQ=255)                                                                                                                           gTAACTGCGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGATCAGGTTATGCATTTCATTTGGGTCATTACGGCGATCGTAAAGTtcat          <  1:118533/140‑1 (MQ=255)                                                                                                                           gTAACTGCGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGATCAGGTTATGCATTTCATTTGGGTCATTACGGCGATCGTAAAGTtcat          <  2:813313/140‑1 (MQ=255)                                                                                                                           gTAACTGCGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGATCAGGTTATGCATTTCATTTGGGTCATTACGGCGATCGTAAAGTtcat          <  2:385960/140‑1 (MQ=255)                                                                                                                                tGCGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGATCAGGTTATGCATTTCATTTGGGTCATTACGGCGATCGTa                  >  1:565894/1‑127 (MQ=255)                                                                                                                                tGCGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGATCAGGTTATGCATTTCATTTGGGTCATTACGGCGATCGTa                  <  2:784712/127‑1 (MQ=255)                                                                                                                                tGCGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGATCAGGTTATGCATTTCATTTGGGTCATTACGGCGATCGTa                  >  1:784712/1‑127 (MQ=255)                                                                                                                                tGCGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGATCAGGTTATGCATTTCATTTGGGTCATTACGGCGATCGTa                  <  2:565894/127‑1 (MQ=255)                                                                                                                                tGCGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGATCAGGTTATGCATTTCATTTGGGTCATTACGGCGATCGTAAAGTTCATCACTg     >  1:714851/1‑140 (MQ=255)                                                                                                                                tGCGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGATCAGGTTATGCATTTCATTTGGGTCATTACGGCGATCGTAAAGTTCATCACTg     >  1:761273/1‑140 (MQ=255)                                                                                                                                tGCGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGATCAGGTTATGCATTTCATTTGGGTCATTACGGCGATCGTAAAGTTCATCACTg     >  1:556447/1‑140 (MQ=255)                                                                                                                                tGCGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGATCAGGTTATGCATTTCATTTGGGTCATTACGGCGATCGTAAAGTTCATCACTg     >  2:311936/1‑140 (MQ=255)                                                                                                                                tGCGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGATCAGGTTATGCATTTCATTTGGGTCATTACGGCGATCGTAAAGTTCATCACTg     >  2:84499/1‑140 (MQ=255)                                                                                                                                 gcggagcggaTCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGATCAGGTTATGCATTTCATTTGGGTCATTACGGCGATCGTAAAGTTCATCACTgg    <  1:452161/140‑1 (MQ=255)                                                                                                                                  cGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGATCAGGTTATGCATTTCATTTGGGTCATTACGGCGATCGTAAAGTTCATCACTGGt   >  1:613790/1‑140 (MQ=255)                                                                                                                                  cGGAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGATCAGGTTATGCATTTCATTTGGGTCATTACGGCGATCGTAAAGTTCATCACTGGt   >  1:822387/1‑140 (MQ=255)                                                                                                                                   ggAGCGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGATCAGGTTATGCATTTCATTTGGGTCATTACGGCGATCGTAAAGTTCATCACTGGTg  <  1:157292/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                         TGTGTCGGTAGGCCGGTGTCATAGTCGCGTACAACTGGCGAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGATCAGGTTATGCATTTCATTTGGGTCATTACGGCGATCGTAAAGTTCATCACTGGTG  >  NC_000913/3856948‑3857217

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑