Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,929,786	A→G	G436G (GGT→GGC)	ravA ←	regulatory ATPase RavA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,929,786	0	A	G	100.0%	199.7 / NA	54	G436G (GGT→GGC)	ravA	regulatory ATPase RavA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/27);  total (27/27)

TCAATGCGACCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAACCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGCCGATACCGTTCAGTTTACCGCGAATTTCACCACCTTTGCTC  >  NC_000913/3929649‑3929921                                                                                                                                          |                                                                                                                                        tCAATGCGACCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAGcc                                                                                                                                       >  2:88831/1‑140 (MQ=255)    aTGCGACCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAGCCggg                                                                                                                                    <  2:652187/140‑1 (MQ=255)           cTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAGCCGGGAAGTGcc                                                                                                                             <  2:390509/140‑1 (MQ=255)                 aGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAGCCGGGAAGTGCCAGCGTa                                                                                                                       <  1:88831/140‑1 (MQ=255)                    cGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAGCCGGGAAGTGCCAGCGTACTg                                                                                                                    >  1:581997/1‑140 (MQ=255)                    cGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAGCCGGGAAGTGCCAGCGTACTg                                                                                                                    <  1:675386/140‑1 (MQ=255)                        aGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAGCCGGGAAGTGCCAGCGTa                                                                                                                       <  2:677406/133‑1 (MQ=255)                        aGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAGCCGGGAAGTGCCAGCGTa                                                                                                                       <  2:619999/133‑1 (MQ=255)                        aGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAGCCGGGAAGTGCCAGCGTa                                                                                                                       >  1:677406/1‑133 (MQ=255)                        aGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAGCCGGGAAGTGCCAGCGTa                                                                                                                       >  1:619999/1‑133 (MQ=255)                         gggATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAGCCGGGAAGTGCCAGCGTACTGCCTTg                                                                                                               <  1:647408/140‑1 (MQ=255)                           gATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAGCCGGGAAGTGCCAGCGTACTGCCTTGTa                                                                                                             <  1:83784/140‑1 (MQ=255)                               aaCAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAGCCGGGAAGTGCCAGCGTACTGCCTTGTAAACt                                                                                                         >  1:733977/1‑140 (MQ=255)                                    aCATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAGCCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACAt                                                                                                    >  1:573957/1‑140 (MQ=255)                                      aTTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAGCCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATcg                                                                                                  <  2:91675/140‑1 (MQ=255)                                        ttttGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAGCCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATcgcg                                                                                                <  1:749913/140‑1 (MQ=255)                                          ttGCTGTTCGCTGAATAAGGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCGTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAGCCGGGAAGTGCCAGCGTACTGACTTGTAAACTTACATCGCGaa                                                                                              <  2:113356/140‑1 (MQ=255)                                          ttGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAGCCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGaa                                                                                              <  2:581997/140‑1 (MQ=255)                                              tGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAGCCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGAc                                                                                          <  2:312576/140‑1 (MQ=255)                                                  cGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAGCCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGa                                                                                      >  2:608005/1‑140 (MQ=255)                                                        aTAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAGCCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTgg                                                                                <  1:242966/140‑1 (MQ=255)                                                            aGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAGCCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGaa                                                                                              >  1:425211/1‑122 (MQ=255)                                                            aGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAGCCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGaa                                                                                              <  2:425211/122‑1 (MQ=255)                                                               gtgtTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAGCCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTGGGTGCTAt                                                                         >  2:593708/1‑140 (MQ=255)                                                                tgtTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAGCCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTGGGCGCTATc                                                                        <  1:768730/140‑1 (MQ=255)                                                                 gtTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAGCCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTGGGCGCTATCa                                                                       >  2:407615/1‑140 (MQ=255)                                                                                        aGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAGCCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGc                                                <  2:573957/140‑1 (MQ=255)                                                                                        aGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAGCCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGc                                                <  1:693961/140‑1 (MQ=255)                                                                                        aGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAGCCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGc                                                <  1:608005/140‑1 (MQ=255)                                                                                            ctTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAGCCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAg                                            <  1:579764/140‑1 (MQ=255)                                                                                             ttCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAGCCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGc                                           >  1:688581/1‑140 (MQ=255)                                                                                             ttCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAGCCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGc                                           <  1:633176/140‑1 (MQ=255)                                                                                                  gttgttgttTTATTTCACCAGGCAGACCTTCAGCCGATGAGCCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGCCGATa                                      <  1:864784/140‑1 (MQ=255)                                                                                                       tgttTTATTTCACCAGGCAGACCTTCAGCCGATGAGCCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGCCGATACCGtt                                 >  1:192035/1‑140 (MQ=255)                                                                                                       tgttTTATTTCACCAGGCAGACCTTCAGCCGATGAGCCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGCCGATACCGtt                                 >  2:33433/1‑140 (MQ=255)                                                                                                        gttTTATTTCACCAGGCAGACCTTCAGCCGATGAGCCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGCCGATACCGTTc                                >  2:364862/1‑140 (MQ=255)                                                                                                        gttTTATTTCACCAGGCAGACCTTCAGCCGATGAGCCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGCCGATACCGTTc                                >  1:314441/1‑140 (MQ=255)                                                                                                           ttATTTCACCAGGCAGACCTTCAGCCGATGAGCCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGCCGATACCGTTCAGt                             <  2:192035/140‑1 (MQ=255)                                                                                                                 cACCAGGCAGACCTTCAGCCGATGAGCCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGCCGATACCGTTCAGTTTACcg                       >  2:112533/1‑140 (MQ=255)                                                                                                                   ccAGGCAGACCTTCAGCCGATGAGCCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTGGGTGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGCCGATACCGTTCAGTTTACcgcg                     <  1:593708/140‑1 (MQ=255)                                                                                                                   ccAGGCAGACCTTCAGCCGATGAGCCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGCCGATACCGTTCAGTTTACcgcg                     >  2:844173/1‑140 (MQ=255)                                                                                                                   ccAGGCAGACCTTCAGCCGATGAGCCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGCCGATACCGTTCAGTTTACcgcg                     >  2:864571/1‑140 (MQ=255)                                                                                                                      ggCAGACCTTCAGCCGATGAGCCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGCCGATACCGTTCAGTTTACCGCGAAt                  >  2:140361/1‑140 (MQ=255)                                                                                                                       gCAGACCTTCAGCCGATGAGCCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGCCGATACCGTTCAGTTTACCGCGAAtt                 >  1:345107/1‑140 (MQ=255)                                                                                                                           aCCTTCAGCCGATGAGCCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGCCGATACCGTTCAGTTTACCGCGAATTTcac             >  2:665740/1‑140 (MQ=255)                                                                                                                           aCCTTCAGCCGATGAGCCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGCCGATACCGTTCAGTTTACCGCGAATTTcac             >  2:804761/1‑140 (MQ=255)                                                                                                                            ccTTCAGCCGATGAGCCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGCCGATACCGTTCAGTTTACCGCGAATTTcacc            >  1:480031/1‑140 (MQ=255)                                                                                                                                cAGCCGATGAGCCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGCCGATACCGTTCAGTTTACCGCGAATTTcac             <  2:507020/135‑1 (MQ=255)                                                                                                                                cAGCCGATGAGCCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGCCGATACCGTTCAGTTTACCGCGAATTTcac             >  1:507020/1‑135 (MQ=255)                                                                                                                                cAGCCGATGAGCCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGCCGATACCGTTCAGTTTACCGCGAATTTCACCACCt        >  1:362646/1‑140 (MQ=255)                                                                                                                                 aGCCGATGAGCCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGCCGATACCGTTCAGTTTACCGCGAATTTCACCACCtt       >  2:211845/1‑140 (MQ=255)                                                                                                                                     gATGAGCCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGCCGATACCGTTCAGTTTACCGCGAATTTCACCACCTTTGCt   <  1:864571/140‑1 (MQ=255)                                                                                                                                      aTGAGCCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGCCGATACCGTTCAGTTTACCGCGAATTTCACCACCTTTGCTc  <  2:741049/140‑1 (MQ=255)                                                                                                                                      aTGAGCCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGCCGATACCGTTCAGTTTACCGCGAATTTCACCACCTTTGCTc  >  1:741049/1‑140 (MQ=255)                                                                                                                                       tGAGCCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGCCGATACCGTTCAGTTTACCGCGAATTTcaccac          <  2:168434/131‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        TCAATGCGACCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAACCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGCCGATACCGTTCAGTTTACCGCGAATTTCACCACCTTTGCTC  >  NC_000913/3929649‑3929921

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑