Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	436,973	T→C	W129R (TGG→CGG)	pgpA →	phosphatidylglycerophosphatase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	436,973	0	T	C	100.0%	109.2 / NA	31	W129R (TGG→CGG)	pgpA	phosphatidylglycerophosphatase A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/14);  total (17/14)

CATTGTCTGGGACGAATTTATTGGTATGTGGATCACGCTCATGGCGCTGCCGACCAATGACTGGCAGTGGGTTGCCGCCGGGTTTGTGATTTTCCGTATTCTGGATATGTGGAAGCCGTGGCCGATCCGCTGGTTTGATCGCAATGTGCATGGCGGCATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAG  >  NC_000913/436855‑437107                                                                                                                       |                                                                                                                                       caTTGTCTGGGACGAATTTATTGGTATGTGGATCACGCTCATGGCGCTGCCGACCAATGACTGGCAGTGGGTTGCCGCCGGGTTTGTGATTTTCCGTATTCTGGATATGTGGAAGCCGCGGCCGATCCGCTGGTTTGATc                                                                                                                   >  2:650135/1‑140 (MQ=255) caTTGTCTGGGACGAATTTATTGGTATGTGGATCACGCTCATGGCGCTGCCGACCAATGACTGGCAGTGGGTTGCCGCCGGGTTTGTGATTTTCCGTATTCTGGATATGTGGAAGCCGCGGCCGATCCGCTGGTTTGATc                                                                                                                   <  1:38450/140‑1 (MQ=255)      tCTGGGACGAATTTATTGGTATGTGGATCACGCTCATGGCGCTGCCGACCAATGACTGGCAGTGGGTTGCCGCCGGGTTTGTGATTTTCCGTATTCTGGATATGTGGAAGCCGCGGCCGATCCGCTGGTTTGATCGCAAt                                                                                                              <  1:767465/140‑1 (MQ=255)      tCTGGGACGAATTTATTGGTATGTGGATCACGCTCATGGCGCTGCCGACCAATGACTGGCAGTGGGTTGCCGCCGGGTTTGTGATTTTCCGTATTCTGGATATGTGGAAGCCGCGGCCGATCCGCTGGTTTGATCGCAAt                                                                                                              >  2:767465/1‑140 (MQ=255)                     ttGGTATGTGGATCACGCTCATGGCGCTGCCGACCAATGACTGGCAGTGGGTTGCCGCCGGGTTTGTGATTTTCCGTATTCTGGATATGTGGAAGCCGCGGCCGATCCGCTGGTTTGATCGCAATGTGCATGGCGGCATg                                                                                               >  2:181016/1‑140 (MQ=255)                          aTGTGGATCACGCTCATGGCGCTGCCGACCAATGACTGGCAGTGGGTTGCCGCCGGGTTTGTGATTTTCCGTATTCTGGATATGTGGAAGCCGCGGCCGATCCGCTGGTTTGATCGCAATGTGCATGGCGGCATGGGGAt                                                                                          >  2:297887/1‑140 (MQ=255)                          aTGTGGATCACGCTCATGGCGCTGCCGACCAATGACTGGCAGTGGGTTGCCGCCGGGTTTGTGATTTTCCGTATTCTGGATATGTGGAAGCCGCGGCCGATCCGCTGGTTTGATCGCAATGTGCATGGCGGCATGGGGAt                                                                                          <  1:650135/140‑1 (MQ=255)                            gtgGATCACGCTCATGGCGCTGCCGACCAATGACTGGCAGTGGGTTGCCGCCGGGTTTGTGATTTTCCGTATTCTGGATATGTGGAAGCCGCGGCCGATCCGCTGGTTTGATCGCAATGTGCATGGCGGCATGGGGATCa                                                                                        <  1:439919/140‑1 (MQ=255)                            gtgGATCACGCTCATGGCGCTGCCGACCAATGACTGGCAGTGGGTTGCCGCCGGGTTTGTGATTTTCCGTATTCTGGATATGTGGAAGCCGCGGCCGATCCGCTGGTTTGATCGCAATGTGCATGGCGGCATGGGGATCa                                                                                        <  2:277364/140‑1 (MQ=255)                             tgGATCACGCTCATGGCGCTGCCGACCAATGACTGGCAGTGGGTTGCCGCCGGGCTTGTGATTTTCCGTATTCTGGATATGTGGAAGCCGCGGCCGATCCGCTGGTTTGATCGCAATGTGCATGGCGGCATGGGGATCAt                                                                                       >  2:480558/1‑140 (MQ=255)                                   aCGCTCATGGCGCTGCCGACCAATGACTGGCAGTGGGTTGCCGCCGGGTTTGTGATTTTCCGTATTCTGGATATGTGGAAGCCGCGGCCGATCCGCTGGTTTGATCGCAATGTGCATGGCGGCATg                                                                                               <  1:760968/126‑1 (MQ=255)                                   aCGCTCATGGCGCTGCCGACCAATGACTGGCAGTGGGTTGCCGCCGGGTTTGTGATTTTCCGTATTCTGGATATGTGGAAGCCGCGGCCGATCCGCTGGTTTGATCGCAATGTGCATGGCGGCATg                                                                                               >  2:760968/1‑126 (MQ=255)                                         aTGGCGCTGCCGACCAATGACTGGCAGTGGGTTGCCGCCGGGTTTGTGATTTTCCGTATTCTGGATATGTGGAAGCCGCGGCCGATCCGCTGGTTTGATCGCAATGTGCATGGCGGCATGGGGATCATGATCGACGatat                                                                           >  2:382005/1‑140 (MQ=255)                                                               ggCAGTGGGTTGCCGCCGGGTTTGTGATTTTCCGTATTCTGGATATGTGGAAGCCGCGGCCGATCCGCTGGTTTGATCGCAATGTGCATGGCGGCATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCa                                                     >  1:709147/1‑140 (MQ=255)                                                                gCAGTGGGTTGCCGCCGGGTTTGTGATTTTCCGTATTCTGGATATGTGGAAGCCGCGGCCGATCCGCTGGTTTGATCGCAATGTGCATGGCGGCATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAg                                                    >  1:854395/1‑140 (MQ=255)                                                                gCAGTGGGTTGCCGCCGGGTTTGTGATTTTCCGTATTCTGGATATGTGGAAGCCGCGGCCGATCCGCTGGTTTGATCGCAATGTGCATGGCGGCATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAg                                                    >  1:431098/1‑140 (MQ=255)                                                                      ggTTGCCGCCGGGTTTGTGATTTTCCGTATTCTGGATATGTGGAAGCCGCGGCCGATCCGCTGGTTTGATCGCAATGTGCATGGCGGCATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATcc                                              <  2:854395/140‑1 (MQ=255)                                                                      ggTTGCCGCCGGGTTTGTGATTTTCCGTATTCTGGATATGTGGAAGCCGCGGCCGATCCGCTGGTTTGATCGCAATGTGCATGGCGGCATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATcc                                              <  2:431098/140‑1 (MQ=255)                                                                        ttGCCGCCGGGTTTGTGATTTTCCGTATTCTGGATATGTGGAAGCCGCGGCCGATCCGCTGGTTTGATCGCAATGTGCATGGCGGCATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTg                                            >  2:334281/1‑140 (MQ=255)                                                                          gccgccGGGTTTGTGATTTTCCGTATTCTGGATATGTGGAAGCCGCGGCCGATCCGCTGGTTTGATCGCAATGTGCATGGCGGCATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTa                                          <  1:181016/140‑1 (MQ=255)                                                                           ccgccgGGTTTGTGATTTTCCGTATTCTGGATATGTGGAAGCCGCGGCCGATCCGCTGGTTTGATCGCAATGTGCATGGCGGCATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTAt                                         >  2:440/1‑140 (MQ=255)                                                                               cgGGTTTGTGATTTTCCGTATTCTGGATATGTGGAAGCCGCGGCCGATCCGCTGGTTTGATCGCAATGTGCATGGCGGCATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTa                                     <  1:382005/140‑1 (MQ=255)                                                                               cgGGTTTGTGATTTTCCGTATTCTGGATATGTGGAAGCCGCGGCCGATCCGCTGGTTTGATCGCAATGTGCATGGCGGCATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTa                                     <  1:297887/140‑1 (MQ=255)                                                                                             tCCGTATTCTGGATATGTGGAAGCCGCGGCCGATCCGCTGGTTTGATCGCAATGTGCATGGCGGCATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTgg                       >  1:237379/1‑140 (MQ=255)                                                                                                 tATTCTGGATATGTGGAAGCCGCGGCCGATCCGCTGGTTTGATCGCAATGTGCATGGCGGCATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGc                   >  1:734221/1‑140 (MQ=255)                                                                                                        gATATGTGGAAGCCGCGGCCGATCCGCTGGTTTGATCGCAATGTGCATGGCGGCATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTAt            <  1:480558/140‑1 (MQ=255)                                                                                                        gATATGTGGAAGCCGCGGCCGATCCGCAGGTTTGATCGCAATGTGCATGGCGGCATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTAt            <  1:622504/140‑1 (MQ=255)                                                                                                             gtgGAAGCCGCGGCCGATCCGCTGGTTTGATCGCAATGTGCATGGCGGCATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGt       >  1:772970/1‑140 (MQ=255)                                                                                                                  aGCCGCGGCCGATCCGCTGGTTTGATCGCAATGTGCATGGCGGCATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCg                    >  2:497919/1‑122 (MQ=255)                                                                                                                  aGCCGCGGCCGATCCGCTGGTTTGATCGCAATGTGCATGGCGGCATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCg                    <  1:497919/122‑1 (MQ=255)                                                                                                                  aGCCGCGGCCGATCCGCTGGTTTGATCGCAATGTGCATGGCGGCATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAg  >  1:648090/1‑140 (MQ=255)                                                                                                                  aGCCGCGGCCGATCCGCTGGTTTGATCGCAATGTGCATGGCGGCATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAg  >  2:95568/1‑140 (MQ=255)                                                                                                                       |                                                                                                                                       CATTGTCTGGGACGAATTTATTGGTATGTGGATCACGCTCATGGCGCTGCCGACCAATGACTGGCAGTGGGTTGCCGCCGGGTTTGTGATTTTCCGTATTCTGGATATGTGGAAGCCGTGGCCGATCCGCTGGTTTGATCGCAATGTGCATGGCGGCATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAG  >  NC_000913/436855‑437107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑