Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,074,149	A→G	V137V (GTA→GTG)	yihV →	6‑deoxy‑6‑sulfofructose kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,074,149	0	A	G	100.0%	231.1 / NA	64	V137V (GTA→GTG)	yihV	6‑deoxy‑6‑sulfofructose kinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/30);  total (34/30)

GGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCAGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTACGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGCCTTTTCAGAAC  >  NC_000913/4074032‑4074285                                                                                                                      |                                                                                                                                         ggtggATACCAAAGGCGAGCGGATAATCATTAACTACCCCAGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTGCGCTGGCACGACGGCGCTaaaa                                                                                                                    <  2:198932/140‑1 (MQ=255)  gtggATACCAAAGGCGAGCGGATAATCATTAACTACCCCAGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTGCGCTGGCACGACGGCGCTaaaaa                                                                                                                   <  2:165699/140‑1 (MQ=255)       tACCAAAGGCGAGCGGATAATCATTAACTACCCCAGCCCGGATGTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGTAGATGTGCGCTGGCACGACGGCGCTAAAAAAGCCt                                                                                                              >  2:647657/1‑140 (MQ=255)       tACCAAAGGCGAGCGGATAATCATTAACTACCCCAGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTGCGCTGGCACGACGGCGCTAAAAAAGCCt                                                                                                              >  2:856976/1‑140 (MQ=255)       tACCAAAGGCGAGCGGATAATCATTAACTACCCCAGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTGCGCTGGCACGACGGCGCTAAAAAAGCCt                                                                                                              >  2:527021/1‑140 (MQ=255)          cAAAGGCGAGCGGATAATCATTAACTACCCCAGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTGCGCTGGCACGACGGCGCTAAAAAAGCCTTCa                                                                                                           >  1:680803/1‑140 (MQ=255)          cAAAGGCGAGCGGATAATCATTAACTACCCCAGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTGCGCTGGCACGACGGCGCTAAAAAAGCCTTCa                                                                                                           >  1:34218/1‑140 (MQ=255)                  aGCGGATAATCATTAACTACCCCAGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTGCGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGcc                                                                                                   >  1:281990/1‑140 (MQ=255)                   gCGGATAATCATTAACTACCCCAGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTGCGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGccc                                                                                                  >  1:321781/1‑140 (MQ=255)                    cGGATAATCATTAACTACCCCAGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTGCGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCg                                                                                                 >  2:511866/1‑140 (MQ=255)                              ttAACTACCCCAGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTGCGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCg                                                                                          <  2:492760/137‑1 (MQ=255)                              ttAACTACCCCAGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTGCGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCg                                                                                          >  1:492760/1‑137 (MQ=255)                              ttAACTACCCCAGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTGCGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGgt                                                                                       >  1:123897/1‑140 (MQ=255)                                 aCTACCCCAGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTGCGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGtgtg                                                                                    >  1:619407/1‑140 (MQ=255)                                 aCTACCCCAGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTGCGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGtgtg                                                                                    >  2:588745/1‑140 (MQ=255)                                     ccccAGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTGCGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCa                                                                                              >  2:284182/1‑126 (MQ=255)                                     ccccAGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTGCGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCa                                                                                              <  1:284182/126‑1 (MQ=255)                                     ccccAGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTGCGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGtgatga                                                                                <  2:619407/140‑1 (MQ=255)                                     ccccAGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTGCGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGtgatga                                                                                >  2:111008/1‑140 (MQ=255)                                     ccccAGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTGCGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGtgatga                                                                                <  1:856976/140‑1 (MQ=255)                                     ccccAGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTGCGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGtgatga                                                                                <  2:123897/140‑1 (MQ=255)                                     ccccAGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTGCGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGtgatga                                                                                >  1:679000/1‑140 (MQ=255)                                     ccccAGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTGCGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGtgatga                                                                                >  2:366060/1‑140 (MQ=255)                                     ccccAGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTGCGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGtgatga                                                                                <  2:458381/140‑1 (MQ=255)                                     ccccAGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTGCGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGtgat                                                                                  <  2:2331/138‑1 (MQ=255)                                     ccccAGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTGCGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGtgat                                                                                  >  1:2331/1‑138 (MQ=255)                                      cccAGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTGCGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGAc                                                                               >  2:135867/1‑140 (MQ=255)                                          gCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTGCGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGtt                                                                           >  1:539711/1‑140 (MQ=255)                                          gCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTGCGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGtt                                                                           <  2:679000/140‑1 (MQ=255)                                          gCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTGCGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGtt                                                                           >  1:208510/1‑140 (MQ=255)                                          gCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTGCGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGtt                                                                           >  1:249697/1‑140 (MQ=255)                                           cccGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTGCGCTGGCACGACGGCGCTAAAAAAGTCTTCACCCTGGCCCGTCAGGCGGGTGTGGTGACCGTTc                                                                          >  2:657299/1‑140 (MQ=255)                                           cccGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTGCGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTc                                                                          <  2:34218/140‑1 (MQ=255)                                            ccGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTGCGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCt                                                                         <  1:765942/140‑1 (MQ=255)                                                aTCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTGCGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGAc                                                                     >  2:390854/1‑140 (MQ=255)                                                aTCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTGCGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGAc                                                                     <  2:360860/140‑1 (MQ=255)                                                 tCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTGCGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACg                                                                    <  1:111008/140‑1 (MQ=255)                                                  ctgctgCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTGCGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACgg                                                                   <  1:390854/140‑1 (MQ=255)                                                  ctgctgCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTGCGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACgg                                                                   <  2:208510/140‑1 (MQ=255)                                                   tgctgcCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTGCGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACggg                                                                  >  1:497068/1‑140 (MQ=255)                                                     ctgcCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTGCGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGa                                                                >  1:762715/1‑140 (MQ=255)                                                               cAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTGCGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGTCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTAcgccg                                                      >  1:457377/1‑140 (MQ=255)                                                               cAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTGCGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTAcgccg                                                      <  1:36038/140‑1 (MQ=255)                                                                    tGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTGCGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTACGCCGCAGGa                                                 <  2:865513/140‑1 (MQ=255)                                                                    tGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTGCGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTACGCCGCAGGa                                                 <  2:249697/140‑1 (MQ=255)                                                                    tGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTGCGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTACGCCGCAGGa                                                 <  1:185358/140‑1 (MQ=255)                                                                       ttGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTGCGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTACGCCGCAGGatat                                              >  2:257363/1‑140 (MQ=255)                                                                         ggaggaAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTGCGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTACGCCGCAGGATATCa                                            >  1:55117/1‑140 (MQ=255)                                                                           aggaAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTGCGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTACGCCGCAGGATATCAGt                                          <  2:860741/140‑1 (MQ=255)                                                                            ggaAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTGCGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTAcgccg                                                      <  2:341328/127‑1 (MQ=255)                                                                            ggaAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTGCGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTAcgccg                                                      >  1:341328/1‑127 (MQ=255)                                                                            ggaAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTGCGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTACGCCGCAGGATATCAGTg                                         >  1:467438/1‑140 (MQ=255)                                                                             gaAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTGCGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTACGCCGCAGGATATCAGTGa                                        <  1:182823/140‑1 (MQ=255)                                                                                aTTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTGCGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTACGCCGCAGGATATCAGTGAGCt                                     >  1:115366/1‑140 (MQ=255)                                                                                 ttGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTGCGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTACGCCGCAGGATATCAGTGAGCtg                                    <  1:647657/140‑1 (MQ=255)                                                                                 ttGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTGCGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTACGCCGCAGGATATCAGTGAGCtg                                    <  1:169536/140‑1 (MQ=255)                                                                                 ttGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTGCGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTACGCCGCAGGATATCAGTGAGCtg                                    <  1:135867/140‑1 (MQ=255)                                                                                     tttCTCTCAGTGGGATGTTGTGCTGGCAGATGTGCGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTACGCCGCAGGATATCAGTGAg                                       >  1:314507/1‑133 (MQ=255)                                                                                     tttCTCTCAGTGGGATGTTGTGCTGGCAGATGTGCGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTACGCCGCAGGATATCAGTGAg                                       <  2:314507/133‑1 (MQ=255)                                                                                                    tgttgtGCTGGCAGATGTGCGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTACGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCAcg                 <  2:762715/140‑1 (MQ=255)                                                                                                      ttgtGCTGGCAGATGTGCGCTGGCACGACGGCGCTAAAAAAGTCTTCACCCTGGCCCGTCAGGCGGGTGTGGTGACCGTTCTGGACGGGGACATTACGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCAcgcg               <  1:657299/140‑1 (MQ=255)                                                                                                            tGGCAGATGTGCGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTACGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGCCttt         <  1:588745/140‑1 (MQ=255)                                                                                                              gCAGATGTGCGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTACGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGCCTTTTc       >  2:492406/1‑140 (MQ=255)                                                                                                                aGATGTGCGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTACGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGCCTTTTCAg     >  1:501639/1‑140 (MQ=255)                                                                                                                   tgtgCGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTACGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGCCTTTTCAGAAc  <  1:511866/140‑1 (MQ=255)                                                                                                                   tgtgCGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTACGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGCCTTTTCAGAAc  <  1:257363/140‑1 (MQ=255)                                                                                                                      |                                                                                                                                         GGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCAGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTACGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGCCTTTTCAGAAC  >  NC_000913/4074032‑4074285

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑