Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,187,063	A→G	T572A (ACG→GCG)	rpoC →	RNA polymerase subunit beta'

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,187,063	0	A	G	100.0%	152.8 / NA	42	T572A (ACG→GCG)	rpoC	RNA polymerase subunit beta'
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/21);  total (21/21)

GACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATTAGTAGCGAAAACCAGCCTGAAAGACACGACTGTTGGCCGTGCCATTCTGTGGATGATTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGT  >  NC_000913/4186930‑4187188                                                                                                                                      |                                                                                                                              gACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATTAGTAGCGAAAACCAGCCTGAAAGACGCGACTg                                                                                                                         <  1:504202/140‑1 (MQ=255)     ggCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATTAGTAGCGAAAACCAGCCTGAAAGACGCGACTGTTgg                                                                                                                     <  2:333549/140‑1 (MQ=255)       cccGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATTAGTAGCGAAAACCAGCCTGAAAGACGCGACTGTTGGcc                                                                                                                   >  2:781338/1‑140 (MQ=255)       cccGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATTAGTAGCGAAAACCAGCCTGAAAGACGCGACTGTTGGcc                                                                                                                   >  2:179120/1‑140 (MQ=255)       cccGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATTAGTAGCGAAAACCAGCCTGAAAGACGCGACTGTTGGcc                                                                                                                   >  1:285593/1‑140 (MQ=255)       cccGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATTAGTAGCGAAAACCAGCCTGAAAGACGCGACTGTTGGcc                                                                                                                   >  2:653968/1‑140 (MQ=255)       cccGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATTAGTAGCGAAAACCAGCCTGAAAGACGCGACTGTTGGcc                                                                                                                   >  2:378339/1‑140 (MQ=255)          gAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATTAGTAGCGAAAACCAGCCTGAAAGACGCGACTGTTGGCCGTg                                                                                                                <  2:138182/140‑1 (MQ=255)                agCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATTAGTAGCGAAAACCAGCCTGAAAGACGCGACTGTTGGCCGTGCCATTc                                                                                                          >  2:576527/1‑140 (MQ=255)                  cAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATTAGTAGCGAAAACCAGCCTGAAAGACGCGACTGTTGGCCGTGCCATTCtg                                                                                                        >  2:328501/1‑140 (MQ=255)                      aCGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATTAGTAGCGAAAACCAGCCTGAAAGACGCGACTGTTGGCCGTGCCATTCTGTGga                                                                                                    <  2:182230/140‑1 (MQ=255)                         tCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATTAGTAGCGAAAACCAGCCTGAAAGACGCGACTGTTGGCCGTGCCATTCTGTGgatga                                                                                                 <  2:164214/140‑1 (MQ=255)                                   tctggtctggCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATTAGTAGCGAAAACCAGCCTGAAAGACGCGACTGTTGGCCGTGCCATTCTGTGGATGATTGTACCGaa                                                                                       >  2:51030/1‑140 (MQ=255)                                   tctggtctggCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATTAGTAGCGAAAACCAGCCTGAAAGACGCGACTGTTGGCCGTGCCATTCTGTGGATGATTGTACCGaa                                                                                       <  1:51030/140‑1 (MQ=255)                                       gtctggCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATTAGTAGCGAAAACCAGCCTGAAAGACGCGACTGTTGGCCGTGCCATTCTGTGGATGATTGTACCGAAAGGt                                                                                   >  1:216544/1‑140 (MQ=255)                                        tctggCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATTAGTAGCGAAAACCAGCCTGAAAGACGCGACTGTTGGCCGTGCCATTCTGTGGATGATTGTACCGAAAGGTc                                                                                  <  2:747149/140‑1 (MQ=255)                                          tggCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATTAGTAGCGAAAACCAGCCTGAAAGACGCGACTGTTGGCCGTGCCATTCTGTGGATGATTGTACCGAAAGGTCTg                                                                                <  2:285593/140‑1 (MQ=255)                                          tggCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATTAGTAGCGAAAACCAGCCTGAAAGACGCGACTGTTGGCCGTGCCATTCTGTGGATGATTGTACCGAAAGGTCTg                                                                                <  2:216544/140‑1 (MQ=255)                                            gCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATTAGTAGCGAAAACCAGCCTGAAAGACGCGACTGTTGGCCGTGCCATTCTGTGGATGATTGTACCGAAAGGTCTGcc                                                                              >  2:314609/1‑140 (MQ=255)                                                 tctGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATTAGTAGCGAAAACCAGCCTGAAAGACGCGACTGTTGGCCGTGCCATTCTGTGGATGATTGTACCGa                                                                                        >  2:374115/1‑125 (MQ=255)                                                 tctGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATTAGTAGCGAAAACCAGCCTGAAAGACGCGACTGTTGGCCGTGCCATTCTGTGGATGATTGTACCGa                                                                                        <  1:374115/125‑1 (MQ=255)                                                 tctGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATTAGTAGCGAAAACCAGCCTGAAAGACGCGACTGTTGGCCGTGCCATTCTGTGGATGATTGTACCGAAAGGTCTGCCTTACt                                                                         >  2:355203/1‑140 (MQ=255)                                                 tctGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATTAGTAGCGAAAACCAGCCTGAAAGACGCGACTGTTGGCCGTGCCATTCTGTGGATGATTGTACCGAAAGGTCTGCCTTACt                                                                         <  1:328501/140‑1 (MQ=255)                                                         cgcgcgTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATTAGTAGCGAAAACCAGCCTGAAAGACGCGACTGTTGGCCGTGCCATTCTGTGGATGATTGTACCGAAAGGTCTGCCTTACTCCATCGTc                                                                 >  2:806720/1‑140 (MQ=255)                                                           cgcgTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATTAGTAGCGAAAACCAGCCTGAAAGACGCGACTGTTGGCCGTGCCATTCTGTGGATGATTGTACCGAAAGGTCTGCCTTACTCCATCGTCaa                                                               >  2:392031/1‑140 (MQ=255)                                                                tAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATTAGTAGCGAAAACCAGCCTGAAAGACGCGACTGTTGGCCGTGCCATTCTGTGGATGATTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAgg                                                          <  1:378339/140‑1 (MQ=255)                                                                tAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATTAGTAGCGAAAACCAGCCTGAAAGACGCGACTGTTGGCCGTGCCATTCTGTGGATGATTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAgg                                                          <  1:439243/140‑1 (MQ=255)                                                                    gTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATTAGTAGCGAAAACCAGCCTGAAAGACGCGACTGTTGGCCGTGCCATTCTGTGGATGATTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCt                                                      <  1:392031/140‑1 (MQ=255)                                                                               cGAGTATGAAAAAGATGCTAACGGTGAATTAGTAGCGAAAACCAGCCTGAAAGACGCGACTGTTGGCCGTGCCATTCTGTGGATGATTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAg                                           >  1:595343/1‑140 (MQ=255)                                                                               cGAGTATGAAAAAGATGCTAACGGTGAATTAGTAGCGAAAACCAGCCTGAAAGACGCGACTGTTGGCCGTGCCATTCTGTGGATGATTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAg                                           >  1:460764/1‑140 (MQ=255)                                                                                 aGTATGAAAAAGATGCTAACGGTGAATTAGTAGCGAAAACCAGCCTGAAAGACGCGACTGTTGGCCGTGCCATTCTGTGGATGATTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCa                                         >  1:144264/1‑140 (MQ=255)                                                                                   tATGAAAAAGATGCTAACGGTGAATTAGTAGCGAAAACCAGCCTGAAAGACGCGACTGTTGGCCGTGCCATTCTGTGGATGATTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAAt                                       >  1:716085/1‑140 (MQ=255)                                                                                          aaGATGCTAACGGTGAATTAGTAGCGAAAACCAGCCTGAAAGACGCGACTGTTGGCCGTGCCATTCTGTGGATGATTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCaaa                                <  2:460764/140‑1 (MQ=255)                                                                                                   aCGGTGAATTAGTAGCGAAAACCAGCCTGAAAGACGCGACTGTTGGCCGTGCCATTCTGTGGATGATTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAac                       <  1:806720/140‑1 (MQ=255)                                                                                                        gAATTAGTAGCGAAAACCAGCCTGAAAGACGCGACTGTTGGCCGTGCCATTCTGTGGATGATTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTg                  <  2:716085/140‑1 (MQ=255)                                                                                                        gAATTAGTAGCGAAAACCAGCCTGAAAGACGCGACTGTTGGCCGCGCCATTCTGTGGATGATTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTg                  <  1:695856/140‑1 (MQ=255)                                                                                                         aaTTAGTAGCGAAAACCAGCCTGAAAGACGCGACTGTTGGCCGTGCCATTCTGTGGATGATTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGc                 >  1:124885/1‑140 (MQ=255)                                                                                                                 gCGAAAACCAGCCTGAAAGACGCGACTGTTGGCCGTGCCATTCTGTGGATGATTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCAt         >  2:79134/1‑140 (MQ=255)                                                                                                                    aaaaCCAGCCTGAAAGACGCGACTGTTGGCCGTGCCATTCTGTGGATGATTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATtct      >  1:485103/1‑140 (MQ=255)                                                                                                                    aaaaCCAGCCTGAAAGACGCGACTGTTGGCCGTGCCATTCTGTGGATGATTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATtct      <  1:57429/140‑1 (MQ=255)                                                                                                                    aaaaCCAGCCTGAAAGACGCGACTGTTGGCCGTGCCATTCTGTGGATGATTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATtct      <  1:179120/140‑1 (MQ=255)                                                                                                                        ccAGCCTGAAAGACGCGACTGTTGGCCGTGCCATTCTGTGGATGATTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGt  <  1:355203/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                              GACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATTAGTAGCGAAAACCAGCCTGAAAGACACGACTGTTGGCCGTGCCATTCTGTGGATGATTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGT  >  NC_000913/4186930‑4187188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑