Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,438,087	T→C	R127R (CGT→CGC)	ytfI →	protein YtfI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,438,087	0	T	C	100.0%	267.7 / NA	72	R127R (CGT→CGC)	ytfI	protein YtfI
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (40/32);  total (40/32)

TAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGG  >  NC_000913/4437952‑4438223                                                                                                                                        |                                                                                                                                         tAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGCGAAt                                                                                                                                      <  1:809974/140‑1 (MQ=255)   aGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGCGAAttt                                                                                                                                    >  1:820915/1‑140 (MQ=255)       tCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGCGAATTTCCGt                                                                                                                                <  1:233358/140‑1 (MQ=255)          tATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGCGAATTTCCGTTAg                                                                                                                             >  1:384719/1‑140 (MQ=255)                  tCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGCGAATTTCCGTTAGAACAAAAc                                                                                                                     >  2:668202/1‑140 (MQ=255)                     gCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGCGAATTTCCGTTAGAACAAAACAGa                                                                                                                  <  1:15052/140‑1 (MQ=255)                     gCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGCGAATTTCCGTTAGAACAAAACAGa                                                                                                                  >  1:192651/1‑140 (MQ=255)                     gCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGCGAATTTCCGTTAGAACAAAACAGa                                                                                                                  >  2:380084/1‑140 (MQ=255)                          aaaGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGCGAATTTCCGTTAGAACAAAACAGACGAtt                                                                                                             >  2:367270/1‑140 (MQ=255)                                aaCGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGCGAATTTCCGTTAGAACAAAACAGACGATTGTATGc                                                                                                       >  2:95390/1‑140 (MQ=255)                                aaCGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGCGAATTTCCGTTAGAACAAAACAGACGATTGTATGc                                                                                                       <  1:95390/140‑1 (MQ=255)                                aaCGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGCGAATTTCCGTTAGAACAAAACAGACGATTGTATGc                                                                                                       >  1:413018/1‑140 (MQ=255)                                 aCGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGCGAATTTCCGTTAGAACAAAACAGACGATTGTATGcc                                                                                                      >  1:529419/1‑140 (MQ=255)                                  cGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGTATTTCAAAAACCGCGAATTTCCGTTAGAACAAAACAGACGATTGTATGcc                                                                                                      <  1:270576/139‑1 (MQ=255)                                  cGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGTATTTCAAAAACCGCGAATTTCCGTTAGAACAAAACAGACGATTGTATGcc                                                                                                      >  2:270576/1‑139 (MQ=255)                                  cGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGCGAATTTCCGTTAGAACAAAACAGACGATTGTATGcc                                                                                                      >  2:640314/1‑139 (MQ=255)                                  cGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGCGAATTTCCGTTAGAACAAAACAGACGATTGTATGcc                                                                                                      <  1:640314/139‑1 (MQ=255)                                     tGCCGAATATACAATGACATTGCGAGGTAGTGGATTTTAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGCGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTggg                                                                                                  >  1:310096/1‑140 (MQ=255)                                     tGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGCGAATTTCCGTTAGAACAGAACAGACGATTGTATGCCTggg                                                                                                  >  1:612022/1‑140 (MQ=255)                                     tGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGCGAATTTCCGTTAGAACAAAACAGACGATTg                                                                                                            <  1:292344/130‑1 (MQ=255)                                     tGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGCGAATTTCCGTTAGAACAAAACAGACGATTg                                                                                                            >  2:292344/1‑130 (MQ=255)                                     tGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGCGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTggg                                                                                                  >  2:638814/1‑140 (MQ=255)                                      gCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGCGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGa                                                                                                 >  1:186042/1‑140 (MQ=255)                                          aaTATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGCGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATAtt                                                                                             >  1:322693/1‑140 (MQ=255)                                                        ttGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGCGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACa                                                                               <  1:367270/140‑1 (MQ=255)                                                        ttGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAAAGAATATAACTCATTCATGGATTTCAAAAACCGCGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACa                                                                               >  1:256281/1‑140 (MQ=255)                                                         tGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGCGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAg                                                                              <  2:89531/140‑1 (MQ=255)                                                         tGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGCGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAg                                                                              >  1:246003/1‑140 (MQ=255)                                                         tGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGCGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAg                                                                              >  2:89294/1‑140 (MQ=255)                                                           cGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGCGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTc                                                                            >  2:23977/1‑140 (MQ=255)                                                              ggTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGCGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGa                                                                         <  2:547885/140‑1 (MQ=255)                                                               gTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGCGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGAc                                                                        >  2:739105/1‑140 (MQ=255)                                                                                gaagaaATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGCGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTaa                                                       <  2:322693/140‑1 (MQ=255)                                                                                gaagaaATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGCGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTaa                                                       >  1:266632/1‑140 (MQ=255)                                                                                gaagaaATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGCGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTaa                                                       <  2:186042/140‑1 (MQ=255)                                                                                  agaaATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGCGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAg                                                     <  1:756344/140‑1 (MQ=255)                                                                                   gaaATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGCGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAgg                                                    <  1:380084/140‑1 (MQ=255)                                                                                            aTAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGCGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCa                                           >  1:83378/1‑140 (MQ=255)                                                                                              aaCAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGCGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCAcc                                         >  1:133645/1‑140 (MQ=255)                                                                                              aaCAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGCGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCAcc                                         >  1:320627/1‑140 (MQ=255)                                                                                              aaCAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGCGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCAcc                                         >  2:384943/1‑140 (MQ=255)                                                                                                 aaTAAATGAATATAACTCATTCATGGATTTCAAAAACCGCGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTtat                                                <  1:129141/130‑1 (MQ=255)                                                                                                 aaTAAATGAATATAACTCATTCATGGATTTCAAAAACCGCGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTtat                                                >  2:129141/1‑130 (MQ=255)                                                                                                 aaTAAATGAATATAACTCATTCATGGATTTCAAAAACCGCGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAgg                                      <  2:192651/140‑1 (MQ=255)                                                                                                 aaTAAATGAATATAACTCATTCATGGATTTCAAAAACCGCGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAgg                                      <  2:386329/140‑1 (MQ=255)                                                                                                           tataACTCATTCATGGATTTCAAAAACCGCGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGa                            >  1:226209/1‑140 (MQ=255)                                                                                                           tataACTCATTCATGGATTTCAAAAACCGCGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGa                            >  2:501698/1‑140 (MQ=255)                                                                                                            ataACTCATTCATGGATTTCAAAAACCGCGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGAc                           <  1:501698/140‑1 (MQ=255)                                                                                                             taACTCATTCATGGATTTCAAAAACCGCGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACg                          <  2:44215/140‑1 (MQ=255)                                                                                                             taACTCATTCATGGATTTCAAAAACCGCGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACg                          <  1:708134/140‑1 (MQ=255)                                                                                                                cTCATTCATGGATTTCAAAAACCGCGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGAc                           <  1:571252/136‑1 (MQ=255)                                                                                                                cTCATTCATGGATTTCAAAAACCGCGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGAc                           >  2:571252/1‑136 (MQ=255)                                                                                                                cTCATTCATGGATTTCAAAAACCGCGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACgtgt                       >  1:267860/1‑140 (MQ=255)                                                                                                                cTCATTCATGGATTTCAAAAACCGCGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACgtgt                       >  1:589766/1‑140 (MQ=255)                                                                                                                    ttcatGGATTTCAAAAACCGCGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCttt                   >  2:469384/1‑140 (MQ=255)                                                                                                                     tcatGGATTTCAAAAACCGCGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTa                  >  2:346304/1‑140 (MQ=255)                                                                                                                        tGGATTTCAAAAACCGCGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCt                     >  1:344160/1‑134 (MQ=255)                                                                                                                        tGGATTTCAAAAACCGCGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCt                     <  2:344160/134‑1 (MQ=255)                                                                                                                        tGGATTTCAAAAACCGCGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGt               >  1:577638/1‑140 (MQ=255)                                                                                                                         ggATTTCAAAAACCGCGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGAc                           <  1:581349/127‑1 (MQ=255)                                                                                                                         ggATTTCAAAAACCGCGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGAc                           >  2:581349/1‑127 (MQ=255)                                                                                                                          gATTTCAAAAACCGCGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTa             <  2:363463/140‑1 (MQ=255)                                                                                                                             ttCAAAAACCGCGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGc          <  1:23977/140‑1 (MQ=255)                                                                                                                             ttCAAAAACCGCGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGc          >  1:1686/1‑140 (MQ=255)                                                                                                                             ttCAAAAACCGCGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGc          >  2:181297/1‑140 (MQ=255)                                                                                                                                   aaCCGCGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTAtt    <  2:83378/140‑1 (MQ=255)                                                                                                                                    aCCGCGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTg   <  2:356075/140‑1 (MQ=255)                                                                                                                                     ccGCGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTTACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTgg  <  2:529419/140‑1 (MQ=255)                                                                                                                                     ccGCGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTa      <  1:777647/136‑1 (MQ=255)                                                                                                                                     ccGCGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTgg  <  2:612022/140‑1 (MQ=255)                                                                                                                                     ccGCGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTgg  <  2:395080/140‑1 (MQ=255)                                                                                                                                     ccGCGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTgg  <  2:820915/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         TAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGG  >  NC_000913/4437952‑4438223

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑