Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,546,320	A→G	T409A (ACA→GCA)	fimD →	type I fimbriae usher protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,546,320	0	A	G	100.0%	263.4 / NA	72	T409A (ACA→GCA)	fimD	type I fimbriae usher protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (38/34);  total (38/34)

CAGTCCCGCTTTTGCAACGTGAAGGGCATACTCGTTATTCCATTACGGCAGGAGAATACCGTAGTGGAAATGCGCAGCAGGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGACAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGGGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGC  >  NC_000913/4546186‑4546455                                                                                                                                       |                                                                                                                                        cAGTCCCGCTTTTGCAACGTGAAGGGCATACTCGTTATTCCATTACGGCAGGAGAATACCGTAGTGGAAATGCGCAGCAGGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGGCAata                                                                                                                                    >  2:180893/1‑140 (MQ=255)  aGTCCCGCTTTTGCAACGTGAAGGGCATACTCGTTATTCCATTACGGCAGGAGAATACCGTAGTGGAAATGCGCAGCAGGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGGCAatat                                                                                                                                   >  1:623408/1‑140 (MQ=255)    tCCCGCTTTTGCAACGTGAAGGGCATACTCGTTATTCCATTACGGCAGGAGAATACCGTAGTGGAAATGCGCAGCAGGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGGCAatatat                                                                                                                                 >  2:168019/1‑140 (MQ=255)                    tGAAGGGCATACTCGTTATTCCATTACGGCAGGAGAATACCGTAGTGGAAATGCGCAGCAGGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGGCAATATATGGTGGAACGCAACTgg                                                                                                                 >  2:32416/1‑140 (MQ=255)                      aaGGGCATACTCGTTATTCCATTACGGCAGGAGAATACCGTAGTGGAAATGCGCAGCAGGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGGCAATATATGGTGGAACGCAACTGGCg                                                                                                               <  2:449513/140‑1 (MQ=255)                               cTCGTTATTCTATTCCGGCAGGAAAATCCCGAAGTGGAAAGGGGCAG‑AAGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGGCAATATATGGTGGAACGCAACTGGCGGATCGTTATc                                                                                                     <  2:463621/140‑1 (MQ=255)                                tCGTTATTCCATTACGGCAGGAGAATACCGTAGTGGAAATGCGCAGCAGGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGGCAATATATGGTGGAACGCAACTGGCGGATCGTTATc                                                                                                     >  2:678231/1‑140 (MQ=255)                                    tATTCCATTACGGCAGGAGAATACCGTAGTGGAAATGCGCAGCAGGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGGCAATATATGGTGGAACGCAACTGGCGGATCGTTATc                                                                                                     <  1:794640/136‑1 (MQ=255)                                    tATTCCATTACGGCAGGAGAATACCGTAGTGGAAATGCGCAGCAGGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGGCAATATATGGTGGAACGCAACTGGCGGATCGTTATc                                                                                                     >  2:794640/1‑136 (MQ=255)                                        ccATTACGGCAGGAGAATACCGTAGTGGAAATGCGCAGCAGGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGGCAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCtttt                                                                                             <  2:216902/140‑1 (MQ=255)                                            tACGGCAGGAGAATACCGTAGTGGAAATGCGCAGCAGGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGGCAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAAtt                                                                                         >  1:363769/1‑140 (MQ=255)                                            tACGGCAGGAGAATACCGTAGTGGAAATGCGCAGCAGGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGGCAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAAtt                                                                                         >  2:285646/1‑140 (MQ=255)                                            tACGGCAGGAGAATACCGTAGTGGAAATGCGCAGCAGGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGGCAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAAtt                                                                                         >  2:812945/1‑140 (MQ=255)                                              cGGCAGGAGAATACCGTAGTGGAAATGCGCAGCAGGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGGCAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTc                                                                                       >  1:183698/1‑140 (MQ=255)                                               ggCAGGAGAATACCGTAGTGGAAATGCGCAGCAGGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGGCAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCg                                                                                      <  1:285646/140‑1 (MQ=255)                                                gCAGGAGAATACCGTAGTGGAAATGCGCAGCAGGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGGCAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCgg                                                                                     >  1:164539/1‑140 (MQ=255)                                                gCAGGAGAATACCGTAGTGGAAATGCGCAGCAGGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGGCAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCgg                                                                                     >  2:624634/1‑140 (MQ=255)                                                 cAGGAGAATACCGTAGTGGAAATGCGCAGCAGGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGGCAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGt                                                                                    <  1:817324/140‑1 (MQ=255)                                                 cAGGAGAATACCGTAGTGGAAATGCGCAGCAGGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGGCAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGt                                                                                    >  1:806681/1‑140 (MQ=255)                                                            cGTAGTGGAAATGCGCAGCAGGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGGCAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGaaaaa                                                                         >  1:347610/1‑140 (MQ=255)                                                            cGTAGTGGAAATGCGCAGCAGGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGGCAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGaaaaa                                                                         >  1:277185/1‑140 (MQ=255)                                                              tAGTGGAAATGCGCAGCAGGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGGTGGCTGGGCAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACa                                                                       >  2:759753/1‑140 (MQ=255)                                                              tAGTGGAAATGCGCAGCAGGAAAAAACCCGCTTTTTCCAGAGTACATTACTACACGGCCTTCCGGCTGGCTGGGCAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACa                                                                       >  1:159001/1‑140 (MQ=255)                                                                gTGGAAATGCGCAGCAGGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGGCAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATg                                                                     <  2:363769/140‑1 (MQ=255)                                                                 tGGAAATGCGCAGCAGGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGGCAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATgg                                                                    >  1:750822/1‑140 (MQ=255)                                                                 tGGAAATGCGCAGCAGGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGGCAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATgg                                                                    >  1:558483/1‑140 (MQ=255)                                                                 tGGAAATGCGCAGCAGGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGGCAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATgg                                                                    >  2:749446/1‑140 (MQ=255)                                                                   gAAATGCGCAGCAGGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGGCAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATgggg                                                                  >  1:509478/1‑140 (MQ=255)                                                                      aTGCGCAGCAGGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGGCAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGGGCa                                                               >  1:466828/1‑140 (MQ=255)                                                                       tGCGCAGCAGGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGGCAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGGGCAc                                                              <  1:766923/140‑1 (MQ=255)                                                                        gcgcAGCAGGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGGCAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGGGCa                                                               >  2:451088/1‑138 (MQ=255)                                                                        gcgcAGCAGGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGGCAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGGGCa                                                               <  1:451088/138‑1 (MQ=255)                                                                        gcgcAGCAGGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGGCAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGGGCACt                                                             >  2:767857/1‑140 (MQ=255)                                                                         cgcAGCAGGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGGCAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGGGCACTg                                                            >  2:553510/1‑140 (MQ=255)                                                                          gcagcaGGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGGCAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGGGCACTgg                                                           >  1:793852/1‑140 (MQ=255)                                                                          gcagcaGGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGGCAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGGGCACTgg                                                           >  2:580636/1‑140 (MQ=255)                                                                           cagcagGAAAAACCCCGCTGTTTCCAGACTTCATTACTCCACGGCCTTCCGGCTGGCTGGGCAATACATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGGGCACTggg                                                          <  2:654472/140‑1 (MQ=255)                                                                             gcagGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGGCAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGGGCACTGGgcg                                                        <  2:509478/140‑1 (MQ=255)                                                                             gcagGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGGCAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGGGCACTGGgcg                                                        >  1:654472/1‑140 (MQ=255)                                                                             gcagGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGGCAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGGGCACTGGgcg                                                        >  1:436140/1‑140 (MQ=255)                                                                              cagGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGGCAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGGGCACTGGgcgc                                                       >  2:87109/1‑140 (MQ=255)                                                                               agGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGGCAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGGGCACTGGGCGct                                                      <  2:277185/140‑1 (MQ=255)                                                                               agGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGGCAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGGGCACTGGGCGct                                                      <  1:32416/140‑1 (MQ=255)                                                                               agGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGGCAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGGGCACTGGGCGct                                                      <  2:164539/140‑1 (MQ=255)                                                                               agGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGGCAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGGGCACTGGGCGct                                                      <  2:347610/140‑1 (MQ=255)                                                                               agGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGGCAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGGGCACTGGGCGct                                                      <  2:750822/140‑1 (MQ=255)                                                                               agGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGGCAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGGGCACTGGGCGct                                                      <  2:436140/140‑1 (MQ=255)                                                                               agGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGGCAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGGGCACTGGGCGct                                                      <  1:87109/140‑1 (MQ=255)                                                                               agGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGGCAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGGGCACTGGGCGct                                                      <  1:553510/140‑1 (MQ=255)                                                                               agGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGGCAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGGGCACTGGGCGct                                                      <  1:812945/140‑1 (MQ=255)                                                                               agGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGGCAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGGGCACTGGGCGct                                                      <  2:466828/140‑1 (MQ=255)                                                                               agGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGGCAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGGGCACTGGGCGct                                                      <  1:767857/140‑1 (MQ=255)                                                                               agGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGGCAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGGGCACTGGGCGct                                                      <  1:580636/140‑1 (MQ=255)                                                                               agGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGGCAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGGGCACTGGGCGct                                                      <  1:645412/140‑1 (MQ=255)                                                                               agGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGGCAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGGGCACTGGGCGct                                                      <  2:623408/140‑1 (MQ=255)                                                                                   aaaaaCCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGGCAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGGGCACTGGGCGCtctgt                                                  <  1:168019/140‑1 (MQ=255)                                                                                                     gagTACATTACTCCACGGCCTTCCGGCTGGCTGGGCAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGGGCACTGGGCGCTCTGTCTGTGGATATGACGCAgg                                <  2:254649/140‑1 (MQ=255)                                                                                                          cATTACTCCACGGCCTTCCGGCTGGCTGGGCAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGGGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAAt                           <  1:180893/140‑1 (MQ=255)                                                                                                                  cACGGCCTTCCGGCTGGCTGGGCAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGGGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACt                   >  1:654094/1‑140 (MQ=255)                                                                                                                  cACGGCCTTCCGGCTGGCTGGGCAATATATGGTGGAACGCAACTGGCGGATCGCTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGGGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACt                   >  2:724236/1‑140 (MQ=255)                                                                                                                     cgCCTTCCGGCTGGCTGGGCAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGGTCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTcc                >  2:757714/2‑140 (MQ=255)                                                                                                                        cTTCCGGCTGGCTGGGCAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGGGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGa             <  2:793423/140‑1 (MQ=255)                                                                                                                         ttCCGGCTGGCTGGGCAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGGGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACtt                  <  2:60786/134‑1 (MQ=255)                                                                                                                         ttCCGGCTGGCTGGGCAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGGGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACtt                  >  1:60786/1‑134 (MQ=255)                                                                                                                         ttCCGGCTGGCTGGGCAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGGGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGAt            <  1:816719/140‑1 (MQ=255)                                                                                                                         ttCCGGCTGGCTGGGCAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGGGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGAt            >  2:108194/1‑140 (MQ=255)                                                                                                                         ttCCGGCTGGCTGGGCAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGGGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGAt            <  1:749446/140‑1 (MQ=255)                                                                                                                         ttCCGGCTGGCTGGGCAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGGGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGAt            <  1:356768/140‑1 (MQ=255)                                                                                                                         ttCCGGCTGGCTGGGCAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGGGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGAt            <  2:183698/140‑1 (MQ=255)                                                                                                                         ttCCGGCTGGCTGGGCAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGGGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGAt            >  1:286448/1‑140 (MQ=255)                                                                                                                          tCCGGCTGGCTGGGCAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGGGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATg           >  2:559322/1‑140 (MQ=255)                                                                                                                           ccGGCTGGCTGGGCAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGGGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGa          <  2:558483/140‑1 (MQ=255)                                                                                                                                   ctggGCAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGGGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGc  >  2:856144/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                        CAGTCCCGCTTTTGCAACGTGAAGGGCATACTCGTTATTCCATTACGGCAGGAGAATACCGTAGTGGAAATGCGCAGCAGGAAAAAACCCGCTTTTTCCAGAGTACATTACTCCACGGCCTTCCGGCTGGCTGGACAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGGGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGC  >  NC_000913/4546186‑4546455

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑