Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	441,118	T→C	T76A (ACA→GCA)	xseB ←	exodeoxyribonuclease VII subunit XseB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	441,118	0	T	C	100.0%	116.0 / NA	32	T76A (ACA→GCA)	xseB	exodeoxyribonuclease VII subunit XseB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/15);  total (17/15)

GCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCGTTCAGCGCCTCTTCC  >  NC_000913/440983‑441248                                                                                                                                        |                                                                                                                                   gcgcCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGCaaaa                                                                                                                                <  1:224246/140‑1 (MQ=255)  cgcCATACTGCCTGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGCAAAAg                                                                                                                               >  2:742336/1‑140 (MQ=255)  cgcCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGCAAAAg                                                                                                                               >  1:266911/1‑140 (MQ=255)  cgcCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGCAAAAg                                                                                                                               >  2:521075/1‑140 (MQ=255)   gcCATACTGCATGGTTTCTACTACGGTAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCAGGTGCAAAAgg                                                                                                                              >  2:386639/1‑140 (MQ=255)   gcCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGCAAAAgg                                                                                                                              >  1:195076/1‑140 (MQ=255)    ccATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGCAAAAggg                                                                                                                             <  2:244760/140‑1 (MQ=255)       tACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGCAAAAGGGGtt                                                                                                                          >  2:146364/1‑140 (MQ=255)           gCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGCAAAAGGGGTTagag                                                                                                                      <  1:364684/140‑1 (MQ=255)           gCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGCAAAAGGGGTTagag                                                                                                                      >  2:483457/1‑140 (MQ=255)                 tttCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGCAAAAGGGGTTAGAGAGGCGt                                                                                                                >  1:547863/1‑140 (MQ=255)                    cGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGCAAAAGGGGTTAGAGAGGCGTCtt                                                                                                             <  2:821593/140‑1 (MQ=255)                    cGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGCAAAAGGGGTTAGAGAGGCGTCtt                                                                                                             >  2:246673/1‑140 (MQ=255)                         aCGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGCAAAAGGGGTTAGAGAGGCGTCTTCATTg                                                                                                        <  2:837057/140‑1 (MQ=255)                               gtgtTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGCAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGAc                                                                                                  <  1:521075/140‑1 (MQ=255)                                tgtTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGCAAAAGGGGTTAGAGAGGCGTCtt                                                                                                             <  1:686409/128‑1 (MQ=255)                                tgtTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGCAAAAGGGGTTAGAGAGGCGTCtt                                                                                                             >  2:686409/1‑128 (MQ=255)                                 gtTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGCAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGAcag                                                                                                <  1:483457/140‑1 (MQ=255)                                           ggCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGCAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGt                                                                                      <  1:565088/140‑1 (MQ=255)                                                     cGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGCAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGtt                                                                            <  2:195076/140‑1 (MQ=255)                                                         aaaaCGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGCAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGc                                                                        <  2:125917/140‑1 (MQ=255)                                                                    gcgcCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGCAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAAtt                                                             <  1:575149/140‑1 (MQ=255)                                                                       ccTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGCAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTgg                                                          >  2:7113/1‑140 (MQ=255)                                                                                     ttAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGCAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGt                                            <  1:7113/140‑1 (MQ=255)                                                                                         cGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGCAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCa                                        >  1:497048/1‑140 (MQ=255)                                                                                              gCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGCAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCAGCTGc                                   >  2:353323/1‑140 (MQ=255)                                                                                                           gcGGAAAGTCCATTACTCATTGTCCGGTGCAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGa                      <  1:94596/140‑1 (MQ=255)                                                                                                              gAAAGTCCATTACTCATTGTCCGGTGCAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACt                   >  1:39804/1‑140 (MQ=255)                                                                                                                          cTCATTGTCCGGTGCAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCGTTCAgcgcat       <  1:386639/140‑3 (MQ=255)                                                                                                                           tCATTGTCCGGTGCAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCGTTCAGCGCctc      >  2:116990/1‑140 (MQ=255)                                                                                                                             aTTGTCCGGTGCAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCGTTCAGCGCctcgt    >  2:801035/1‑138 (MQ=255)                                                                                                                             aTTGTCCGGTGCAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCGTTCAGCGCCTCtt    >  2:672636/1‑140 (MQ=255)                                                                                                                               tGTCCGGTGCAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCGTTCAGCGCCTCTTcc  >  2:389769/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                   GCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCGTTCAGCGCCTCTTCC  >  NC_000913/440983‑441248

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑