Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	453,781	G→A	I129I (ATC→ATT)	yajG ←	putative lipoprotein YajG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	453,781	0	G	A	100.0%	130.4 / NA	37	I129I (ATC→ATT)	yajG	putative lipoprotein YajG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/18);  total (19/18)

GTATCGGTCAGCACACTGTTAACCGCATCGGCGATATTTTTGTTGGAGGCCTGGAACGCACCTTCAACGTTGTAGCTGGCACGATAGTTTTTGGTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCGATATCCGCTTTGGTCGCGATGTTGTAGCGCACGTTGCCCTGGGACACGTCAGCATACAGTTGGCTAACGATGATTTGCAGATTAACCGGGCCATTCGGACCAACCATGTAACCACGCGCGGTCATCTGT  >  NC_000913/453650‑453910                                                                                                                                    |                                                                                                                                  gTATCGGTCAGCACACTGTTAACCGCATCGGCGATATTTTTGTTGGAGGCCTGGAACGCACCTTCAACGTTGTAGCTGGCACGATAGTTTTTGGTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCAATATCCGc                                                                                                                           >  1:630849/1‑140 (MQ=255)         cAGCACACTGTTAACCGCATCGGCGATATTTTTGTTGGAGGCCTGGAACGCACCTTCAACGTTGTAGCTGGCACGATAGTTTTTGGTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCAATATCCGCTTTGGTcg                                                                                                                   >  1:228682/1‑140 (MQ=255)          aGCACACTGTTAACCGCATCGGCGATATTTTTGTTGGAGGCCTGGAACGCACCTTCAACGTTGTAGCTGGCACGATAGTTTTTGGTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCAATATCCGCTTTGGTcgc                                                                                                                  <  1:816470/140‑1 (MQ=255)                 tGTTAACCGCATCGGCGATATTTTTGTTGGAGGCCTGGAACGCACCTTCAACGTTGTAGCTGGCACGATAGTTTTTGGTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCAATATCCGCTTTGGTcg                                                                                                                   <  2:272715/132‑1 (MQ=255)                 tGTTAACCGCATCGGCGATATTTTTGTTGGAGGCCTGGAACGCACCTTCAACGTTGTAGCTGGCACGATAGTTTTTGGTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCAATATCCGCTTTGGTcg                                                                                                                   >  1:272715/1‑132 (MQ=255)                 tGTTAACCGCATCGGCGATATTTTTGTTGGAGGCCTGGAACGCACCTTCAACGTTGTAGCTGGCACGATAGTTTTTGGTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCAATATCCGCTTTGGTCGCGAtgttg                                                                                                           >  1:631588/1‑140 (MQ=255)                    tAACCGCATCGGCGATATTTTTGTTGGAGGCCTGGAACGCACCTTCAACGTTGTAGCTGGCACGATAGTTTTTGGTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCAATATCCGCTTTGGTCGCGATGTTGTAg                                                                                                        <  2:630849/140‑1 (MQ=255)                          cATCGGCGATATTTTTGTTGGAGGCCTGGAACGCACCTTCAACGTTGTAGCTGGCACGATAGTTTTTGGTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCAATATCCGCTTTGGTCGCGATGTTGTAGCGCACg                                                                                                  <  2:527584/140‑1 (MQ=255)                          cATCGGCGATATTTTTGTTGGAGGCCTGGAACGCACCTTCAACGTTGTAGCTGGCACGATAGTTTTTGGTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCAATATCCGCTTTGGTCGCGATGTTGTAGCGCACg                                                                                                  <  2:785717/140‑1 (MQ=255)                           aTCGGCGATATTTTTGTTGGAGGCCTGGAACGCACCTTCAACGTTGTAGCTGGCACGATAGTTTTTGGTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCAATATCCGCTTTGGTCGCGATGTTGTAGCGCACGt                                                                                                 <  2:809904/140‑1 (MQ=255)                           aTCGGCGATATTTTTGTTGGAGGCCTGGAACGCACCTTCAACGTTGTAGCTGGCACGATAGTTTTTGGTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCAATATCCGCTTTGGTCGCGATGTTGTAGCGCACGt                                                                                                 >  2:199593/1‑140 (MQ=255)                           aTCGGCGATATTTTTGTTGGAGGCCTGGAACGCACCTTCAACGTTGTAGCTGGCACGATAGTTTTTGGTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCAATATCCGCTTTGGTCGCGATGTTGTAGCGCACGt                                                                                                 >  1:377761/1‑140 (MQ=255)                             cGGCGATATTTTTGTTGGAGGCCTGGAACGCACCTTCAACGTTGTAGCTGGCACGATAGTTTTTGGTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCAATATCCGCTTTGGTCGCGATGTTGTAGCGCACGTTg                                                                                               >  2:598905/1‑140 (MQ=255)                                       tttGTTGGAGGCCTGGAACGCACCTTCAACGTTGTAGCTGGCACGATAGTTTTTGGTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCAATATCCGCTTTGGTCGCGATGTTGTAGCGCACGTTGCCCTGGGaca                                                                                     <  1:238608/140‑1 (MQ=255)                                             ggAGGCCTGGAACGCACCTTCAACGTTGTAGCTGGCACGATAGTTTTTGGTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCAATATCCGCTTTGGTCGCGATGTTGTAGCGCACGTTGCCCTGGGACACGTCAg                                                                               <  1:287143/140‑1 (MQ=255)                                              gAGGCCTGGAACGCACCTTCAACGTTGTAGCTGGCACGATAGTTTTTGGTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCAATATCCGCTTTGGTCGCGATGTTGTAGCGCACGTTGCCCTGGGACACGTCAGc                                                                              <  2:799016/140‑1 (MQ=255)                                                         cGCACCTTCAACGTTGTAGCTGGCACGATAGTTTTTGGTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCAATATCCGCTTTGGTCGCGATGTTGTAGCGCACGTTGCCCTGGGACACGTCAGCATACAGTTGGc                                                                   <  1:77754/140‑1 (MQ=255)                                                           cACCTTCAACGTTGTAGCTGGCACGATAGTTTTTGGTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCAATATCCGCTTTGGTCGCGATGTTGTAGCGCACGTTGCCCTGGGACACGTCAGCATACAGTTGGCTa                                                                 >  2:672915/1‑140 (MQ=255)                                                           cACCTTCAACGTTGTAGCTGGCACGATAGTTTTTGGTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCAATATCCGCTTTGGTCGCGATGTTGTAGCGCACGTTGCCCTGGGACACGTCAGCATACAGTTGGCTa                                                                 >  1:795893/1‑140 (MQ=255)                                                           cACCTTCAACGTTGTAGCTGGCACGATAGTTTTTGGTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCAATATCCGCTTTGGTCGCGATGTTGTAGCGCACGTTGCCCTGGGACACGTCAGCATACAGTTGGCTa                                                                 >  2:467791/1‑140 (MQ=255)                                                            aCCTTCAACGTTGTAGCTGGCACGATAGTTTTTGGTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCAATATCCGCTTTGGTCGCGATGTTGTAGCGCACGTTGCCCTGGGACACGTCAGCa                                                                             >  1:344583/1‑127 (MQ=255)                                                            aCCTTCAACGTTGTAGCTGGCACGATAGTTTTTGGTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCAATATCCGCTTTGGTCGCGATGTTGTAGCGCACGTTGCCCTGGGACACGTCAGCa                                                                             <  2:344583/127‑1 (MQ=255)                                                                    cGTTGTAGCTGGCACGATAGTTTTTGGTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCAATATCCGCTTTGGTCGCGATGTTGTAGCGCACGTTGCCCTGGGACACGTCAGCATACAGTTGGCTAACGATGAtt                                                        <  1:467791/140‑1 (MQ=255)                                                                        gTAGCTGGCACGATAGTTTTTGGTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCAATATCCGCTTTGGTCGCGATGTTGTAGCGCACGTTGCCCTGGGACACGTCAGCATACAGTTGGCTAACGATGATTTGCa                                                    >  1:131205/1‑140 (MQ=255)                                                                        gTAGCTGGCACGATAGTTTTTGGTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCAATATCCGCTTTGGTCGCGATGTTGTAGCGCACGTTGCCCTGGGACACGTCAGCATACAGTTGGCTAACGATGATTTGCa                                                    >  1:540132/1‑140 (MQ=255)                                                                        gTAGCTGGCACGATAGTTTTTGGTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCAATATCCGCTTTGGTCGCGATGTTGTAGCGCACGTTGCCCTGGGACACGTCAGCATACAGTTGGCTAACGATGATTTGCa                                                    >  1:450777/1‑140 (MQ=255)                                                                              ggCACGATAGTTTTTGGTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCAATATCCGCTTTGGTCGCGATGTTGTAGCGCACGTTGCCCTGGGACACGTCAGCATACAGTTGGCTAACGATGATTTGCAGATTaa                                              >  1:708721/1‑140 (MQ=255)                                                                                 aCGATAGTTTTTGGTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCAATATCCGCTTTGGTCGCGATGTTGTAGCGCACGTTGCCCTGGGACACGTCAGCATACAGTTGGCTAACGATGATTTGCAGATTAACCg                                           >  2:426592/1‑140 (MQ=255)                                                                                   gATACTTTTTGGTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCAATATCCGCTTTGGTCGCGATGTTGTAGCGCACGTTGCCCTGGGACACGTCAGCATACAGTTGGCTAACGATGATTTGCAGATTAACCggg                                         <  2:795893/140‑1 (MQ=255)                                                                                              gTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCAATATCCGCTTTGGTCGCGATGTTGTAGCGCACGTTGCCCTGGGACACGTCAGCATACAGTTGGCTAACGATGATTTGCAGATTAACCGGGCCATTCGGacc                              <  2:377761/140‑1 (MQ=255)                                                                                              gTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCAATATCCGCTTTGGTCGCGATGTTGTAGCGCACGTTGCCCTGGGACACGTCAGCATACAGTTGGCTAACGATGATTTGCAGATTAACCGGGCCATTCGGacc                              <  2:228682/140‑1 (MQ=255)                                                                                              gTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCAATATCCACTTTGGTCGCGATGTTGTAGCGCACGTTGCCCTGGGACACGTCAGCATACAGTTGGCTAACGATGATTTGCAGATTAACCGGGCCATTCGGacc                              <  1:121423/140‑1 (MQ=255)                                                                                                             ttCTGCGCGGTAGCGATGATGGCAATATCCGCTTTGGTCGCGATGTTGTAGCGCACGTTGCCCTGGGACACGTCAGCATACAGTTGGCTAACGATGATTTGCAGATTAACCGGGCCATTCGGacc                              <  2:549983/125‑1 (MQ=255)                                                                                                             ttCTGCGCGGTAGCGATGATGGCAATATCCGCTTTGGTCGCGATGTTGTAGCGCACGTTGCCCTGGGACACGTCAGCATACAGTTGGCTAACGATGATTTGCAGATTAACCGGGCCATTCGGacc                              >  1:549983/1‑125 (MQ=255)                                                                                                                       tAGCGATGATGGCAATATCCGCTTTGGTCGCGATGTTGTAGCGCACGTTGCCCTGGGACACGTCAGCATACAGTTGGCTAACGATGATTTGCAGATTAACCGGGCCATTCGGACCAACCATGTAACCACGCGCGGTCATc     >  2:343483/1‑140 (MQ=255)                                                                                                                       tAGCGATGATGGCAATATCCGCTTTGGTCGCGATGTTGTAGCGCACGTTGCCCTGGGACACGTCAGCATACAGTTGGCTAACGATGATTTGAAGATTAACCGGGCCATTCGGACCAACCATGTAACCACGCGCGGTCATc     >  2:167040/1‑140 (MQ=255)                                                                                                                          cGATGATGGCAATATCCGCTTTGGTCGCGATGTTGTAGCGCACGTTGCCCTGGGACACGTCAGCATACAGTTGGCTAACGATGATTTGCAGATTAACCGGGCCATTCGGACCAACCATGTAACCACGCGCGGTCATCTGt  <  2:631588/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                  GTATCGGTCAGCACACTGTTAACCGCATCGGCGATATTTTTGTTGGAGGCCTGGAACGCACCTTCAACGTTGTAGCTGGCACGATAGTTTTTGGTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCGATATCCGCTTTGGTCGCGATGTTGTAGCGCACGTTGCCCTGGGACACGTCAGCATACAGTTGGCTAACGATGATTTGCAGATTAACCGGGCCATTCGGACCAACCATGTAACCACGCGCGGTCATCTGT  >  NC_000913/453650‑453910

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑