Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 516,908 T→C V109A (GTG→GCG)  fetB → putative iron ABC exporter membrane subunit FetB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913516,9080TC100.0% 149.4 / NA 44V109A (GTG→GCG) fetBputative iron ABC exporter membrane subunit FetB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/23);  total (21/23)

CCTGACATTATTGATGGTGTTATTTATCTGCTTTAATGCGGCGTGGAACGCGCAAAAACGCAGTAAATATATTGCTAAAGCTTTTATCTCATCGTTTATTGCCATTACGGTCGGGGCGGGAATTACCCTGGCGGTGCTGATTCTCTCAGGGTCGATTGAATTTATCCCGATGCAGGTGATCCCTATCGCCGGGATGATTGCCGGTAACGCCATGGTAGCGGTGGGGTTGTGTTACAACAATTTAGGGCAACGGGTCATTAGCGAA  >  NC_000913/516774‑517038
                                                                                                                                      |                                                                                                                                  
ccTGACATTATTGATGGTGTTATTTATCTGCTTTAATGCGGCGTGGAACGCGCAAAAACGCAGTAAATATATTGCTAAAGCTTTTATCTCATCGTTTATTGCCATTACGGTCGGGGCGGGAATTACCCTGGCGGCGCTGa                                                                                                                               >  1:545384/1‑140 (MQ=255)
 cTGACATTATTGATGGTGTTATTTATCTGCTTTAATGCGGCGTGGAACGCGCAAAAACGCAGTAAATATATTGCTAAAGCTTTTATCTCATCGTTTATTGCCATTACGGTCGGGGCGGGAATTACCCTGGCGGCGCTGAt                                                                                                                              <  2:810151/140‑1 (MQ=255)
    aCATTGTTGATGGTGTTATTTATCTGCTTTAATGCGGCGTGGAACGCGCAAAAACGCAGTAAATATATTGCTAAAGCTTTTATCTCATCGTTTATTGCCATTACGGTCGGGGCGGGAATTACCCTGGCGGCGCTGATtct                                                                                                                           >  1:449003/1‑140 (MQ=255)
          tcgATGGTGTTATTTATCTGCTTTAATGCGGCGTGGAACGCGCAAAAACGCAGTAAATATATTGCTAAAGCTTTTATCTCATCGTTTATTGCCATTACGGTCGGGGCGGGAATTACCCTGGCGGCGCTGATTCTCTCAgg                                                                                                                     >  2:180232/3‑140 (MQ=255)
                      tttatCTGCTTTAATGCGGCGTGGAACGCGCAAAAACGCAGTAAATATATTGCTAAAGCTTTTATCTCATCGTTTATTGCCATTACGGTCGGGGCGGGAATTACCCTGGCGGCGCTGATTCTCTCAGGGTCGATTGAAtt                                                                                                         <  1:160398/140‑1 (MQ=255)
                         atCTGCTTTAATGCGGCGTGGAACGCGCAAAAACGCAGTAAATATATTGCTAAAGCTTTTATCTCATCGTTTATTGCCATTACGGTCGGGGCGGGAATTACCCTGGCGGCGCTGATTCTCTCAGGGTCGATTGAATTTAt                                                                                                      <  2:201217/140‑1 (MQ=255)
                           cTGCTTTAATGCGGCGTGGAACGCGCAAAAACGCAGTAAATATATTGCTAAAGCTTTTATCTCATCGTTTATTGCCATTACGGTCGGGGCGGGAATTACCCTGGCGGCGCTGATTCTCTCAGGGTCGATTGAATTTATcc                                                                                                    >  1:585240/1‑140 (MQ=255)
                           cTGCTTTAATGCGGCGTGGAACGCGCAAAAACGCAGTAAATATATTGCTAAAGCTTTTATCTCATCGTTTATTGCCATTACGGTCGGGGCGGGAATTACCCTGGCGGCGCTGATTCTCTCAGGGTCGATTGAATTTATcc                                                                                                    <  2:519814/140‑1 (MQ=255)
                           cTGCTTTAATGCAGCGTGGAACGCGCAAAAACGCAGTAAATATATTACTAAAGCTTTTATCTCATCGTTTATTGCCATTACGGTCGGGGCGGGAAATACCCTGGCGGCGCTGATTATCTCACGGTCCGTTTAATTTAttc                                                                                                    >  2:138458/1‑138 (MQ=255)
                                ttAATGCGGCGTGGAACGCGCAAAAACGCAGTAAATATATTGCTAAAGCTTTTATCTCATCGTTTATTGCCATTACGGTCGGGGCGGGAATTACCCTGGCGGCGCTGATTCTCTCAGGGTCGATTGAATTTATCCCGATg                                                                                               <  2:545384/140‑1 (MQ=255)
                                ttAATGCGGCGTGGAACGCGCAAAAACGCAGTAAATATATTGCTAAAGCTTTTATCTCATCGTTTATTGCCATTACGGTCGGGGCGGGAATTACCCTGGCGGCGCTGATTCTCTCAGGGTCGATTGAATTTATCCCGATg                                                                                               <  2:779654/140‑1 (MQ=255)
                                ttAATGCGGCGTGGAACGCGCAAAAACGCAGTAAATATATTGCTAAAGCTTTTATCTCATCGTTTATTGCCATTACGGTCGGGGCGGGAATTACCCTGGCGGCGCTGATTCTCTCAGGGTCGATTGAATTTATCCCGATg                                                                                               <  1:180232/140‑1 (MQ=255)
                                ttAATGCGGCGTGGAACGCGCAAAAACGCAGCAAATATATTGCTAAAGCTTTTATCTCATCGTTTATTGCCATTACGGTCGGGGCGGGAATTACCCTGGCGGCGCTGATTCTCTCAGGGTCGATTGAATTTATCCCGATg                                                                                               <  1:631561/140‑1 (MQ=255)
                                 tAATGCGGCGTGGAACGCGCAAAAACGCAGTAAATATATTGCTAAAGCTTTTATCTCATCGTTTATTGCCATTACGGTCGGGGCGGGAATTACCCTGGCGGCGCTGATTCTCTCAGGGTCGATTGAATTTATCCCGATGc                                                                                              >  2:386341/1‑140 (MQ=255)
                                        gcgTGGAACGCGCAAAAACGCAGTAAATATATTGCTAAAGCTTTTATCTCATCGTTTATTGCCATTACGGTCGGGGCGGGAATTACCCTGGCGGCGCTGATTCTCTCAGGGTCGATTGAATTTATCCCGATGCAGGTGAt                                                                                       >  2:672098/1‑140 (MQ=255)
                                        gcgTGGAACGCGCAAAAACGCAGTAAATATATTGCTAAAGCTTTTATCTCATCGTTTATTGCCATTACGGTCGGGGCGGGAATTACCCTGGCGGCGCTGATTCTCTCAGGGTCGATTGAATTTATCCCGATGCAGGTGAt                                                                                       <  1:672098/140‑1 (MQ=255)
                                                    cAAAAACGCAGTAAATATATTGCTAAAGCTTTTATCTCATCGTTTATTGCCATTACGGTCGGGGCGGGAATTACCCTGGCGGCGCTGATTCTCTCAGGGTCGATTGAATTTATCCCGATGCAGGTGATCCCTATCGCCgg                                                                           <  1:663739/140‑1 (MQ=255)
                                                        aaCGCAGTAAATATATTGCTAAAGCTTTTATCTCATCGTTTATTGCCATTACGGTCGGGGCGGGAATTACCCTGGCGGCGCTGATTCTCTCAGGGTCGGTTGAATTTATCCCGATGCAGGTGATCCCTATCGCCGGgatg                                                                       >  2:323833/1‑140 (MQ=255)
                                                        aaCGCAGTAAATATATTGCTAAAGCTTTTATCTCATCGTTTATTGCCATTACGGTCGGGGCGGGAATTACCCTGGCGGCGCTGATTCTCTCAGGGTCGATTGAATTTATCCCGATGCAGGTGATCCCTATCGCCGGgatg                                                                       >  2:379513/1‑140 (MQ=255)
                                                                 aaTATATTGCTAAAGCTTTTATCTCATCGTTTATTGCCATTACGGTCGGGGCGGGAATTACCCTGGCGGCGCTGATTCTCTCAGGGTCGATTTAATTTATCCCGATGCAGGTGATCCCTATCGCCGGGATGATTGCCGGt                                                              <  1:138458/140‑1 (MQ=255)
                                                                 aaTATATTGCTAAAGCTTTTATCTCATCGTTTATTGCCATTACGGTCGGGGCGGGAATTACCCTGGCGGCGCTGATTCTCTCAGGGTCGATTGAATTTATCCCGATGCAGGTGATCCCTATCGCCGGGATGATTGCCGGt                                                              <  1:767935/140‑1 (MQ=255)
                                                                    atatTGCTAAAGCTTTTATCTCATCGTTTATTGCCATTACGGTCGGGGCGGGAATTACCCTGGCGGCGCTGATTCTCTCAGGGTCGATTGAATTTATCCCGATGCAGGTGATCCCTATCGCCGGGATGATTGCCGGTAAc                                                           <  1:386341/140‑1 (MQ=255)
                                                                       ttGCTAAAGCTTTTATCTCATCGTTTATTGCCATTACGGTCGGGGCGGGAATTACCCTGGCGGCGCTGATTCTCTCAGGGTCGATTGAATTTATCCCGATGCAGGTGATCCCTATCGCCGGGATGATTGCCGGTAACGcc                                                        >  1:544393/1‑140 (MQ=255)
                                                                         gCTAAAGCTTTTATCTCATCGTTTATTGCCATTACGGTCGGGGCGGGAATTACCCTGGCGGCGCTGATTCTCTCAGGGTCGATTGAATTTATCCCGATGCAGGTGATCCCTATCGCCGGGATGATTGCCGGTAACGCCAt                                                      >  2:164485/1‑140 (MQ=255)
                                                                         gCTAAAGCTTTTATCTCATCGTTTATTGCCATTACGGTCGGGGCGGGAATTACCCTGGCGGCGCTGATTCTCTCAGGGTCGATTGAATTTATCCCGATGCAGGTGATCCCTATCGCCGGGATGATTGCCGGTAACGCCAt                                                      >  1:279815/1‑140 (MQ=255)
                                                                          cTAAAGCTTTTATCTCATCGTTTATTGCCATTACGGTCGGGGCGGGAATTACCCTGGCGGCGCTGATTCTCTCAGGGTCGATTGAATTTATCCCGATGCAGGTGATCCCTATCGCCGGGATGATTGCCGGTAACGCCATg                                                     >  2:199636/1‑140 (MQ=255)
                                                                             aaGCTTTTATCTCATCGTTTATTGCCATTACGGTCGGGGCGGGAATTACCCTGGCGGCGCTGATTCTCTCAGGGTCGATTGAATTTATCCCGATGCAGGTGATCCCTATCGCCGGGATGATTGCCGGTAACGCCATGGTa                                                  >  1:10008/1‑140 (MQ=255)
                                                                                   ttATCTCATCGTTTATTGCCATTACGGTCGGGGCGGGAATTACCCTGGCGGCGCTGATTCTCTCAGGGTCGATTGAATTTATCCCGATGCAGGTGATCCCTATCGCCGGGATGATTGCCGGTAACGCCATGGTAGCGGTg                                            <  2:544393/140‑1 (MQ=255)
                                                                                    tATCTCATCGTTTATTGCCATTACGGTCGGGGCGGGAATTACCCTGGCGGCGCTGATTCTCTCAGGGTCGATTGAATTTATCCCGATGCAGGTGATCCCTATCGCCGGGATGATTGCCGGTAACGCCATGGTAGCGGTgg                                           >  1:175758/1‑140 (MQ=255)
                                                                                       ctcATCGTTTATTGCCATTACGGTCGGGGCGGGAATTACCCTGGCGGCGCTGATTCTCTCAGGGTCGATTGAATTTATCCCGATGCAGGTGATCCCTATCGCCGGGATGATTGCCGGTAACGCCATGGTAGCGGTGGGGt                                        <  2:10008/140‑1 (MQ=255)
                                                                                                 aTTGCCATTACGGTCGGGGCGGGAATTACCCTGGCGGCGCTGATTCTCTCAGGGTCGATTGAATTTATCCCGATGCAGGTGATCCCTATCGCCGGGATGATTGCCGGTAACGCCATGGTAGCGGTGGGGTTGTGTTacaa                              >  1:814872/1‑140 (MQ=255)
                                                                                                 aTTGCCATTACGGTCGGGGCGGGAATTACCCTGGCGGCGCTGATTCTCTCAGGGTCGATTGAATTTATCCCGATGCAGGTGATCCCTATCGCCGGGATGATTGCCGGTAACGCCATGGTAGCGGTGGGGTTGTGTTacaa                              <  2:449003/140‑1 (MQ=255)
                                                                                                 aTTGCCATTACGGTCGGGGCGGGAATTACCCTGGCGGCGCTGATTCTCTCAGGGTCGATTGAATTTATCCCGATGCAGGTGATCCCTATCGCCGGGATGATTGCCGGTAACGCCATGGTAGCGGTGGGGTTGTGTTacaa                              <  2:814872/140‑1 (MQ=255)
                                                                                                        ttACGGTCGGGGCGGGAATTACCCTGGCGGCGCTGATTCTCTCAGGGTCGATTGAATTTATCCCGATGCAGGTGATCCCTATCGCCGGGATGATTGCCGGTAACGCCATGGTAGCGGTGGGGTTGTGTTACAACAATTTa                       >  1:81352/1‑140 (MQ=255)
                                                                                                         tACGGTCGGGGCGGGAATTACCCTGGCGGCGCTGATTCTCTCAGGGTCGATTGAATTTATCCCGATGCAGGTGATCCCTATCGCCGGGATGATTGCCGGTAACGCCATGGTAGCGGTGGGGTTGTGTTACAACAATTTAg                      >  1:239134/1‑140 (MQ=255)
                                                                                                          aCGGTCGGGGCGGGAATTACCCTGGCGGCGCTGATTCTCTCAGGGTCGATTGAATTTATCCCGATGCAGGTGATCCCTATCGCCGGGATGATTGCCGGTAACGCCATGGTAGCGGTGGGGTTGTGTTACAACAATTTAgg                     <  1:379513/140‑1 (MQ=255)
                                                                                                               cGGGGCGGGAATTACCCTGGCGGCGCTGATTCTCTCAGGGTCGATTGAATTTATCCCGATGCAGGTGATCCCTATCGCCGGGATGATTGCCGGTAACGCCATGGTAGCGGTGGGGTTGTGTTACAACAAttt                        >  2:184551/1‑132 (MQ=255)
                                                                                                               cGGGGCGGGAATTACCCTGGCGGCGCTGATTCTCTCAGGGTCGATTGAATTTATCCCGATGCAGGTGATCCCTATCGCCGGGATGATTGCCGGTAACGCCATGGTAGCGGTGGGGTTGTGTTACAACAAttt                        <  1:184551/132‑1 (MQ=255)
                                                                                                                ggggCGGGAATTACCCTGGCGGCGCTGATTCTCTCAGGGTCGATTGAATTTATCCCGATGCAGGTGATCCCTATCGCCGGGATGATTGCCGGTAACGCCATGGTAGCGGTGGGGTTGTGTTACAACAATTTAGGGCAACg               >  2:326871/1‑140 (MQ=255)
                                                                                                                ggggCGGGAATTACCCTGGCGGCGCTGATTCTCTCAGGGTCGATTGAATTTATCCAGATGCAGGTGATCCCTATCGCCGGGATGATTGCCGGTAACGCCATGGTAGCGGTGGGGTTGTGTTACAACAATTTAGGGCAACg               >  2:241202/1‑140 (MQ=255)
                                                                                                                   gCGGGAATTACCCTGGCGGCGCTGATTCTCTCAGGGTCGATTGAATTTATCCAGATGCAGGTGATCCCTATCGCCGGGATGATTGCCGGTAACGCCATGGTAGCGGTGGGGTTGTGTTACAACAATTTAGGGCAACGGGt            <  1:241202/140‑1 (MQ=255)
                                                                                                                        aaTTACCCTGGCGGCGCTGATTCTCTCAGGGTCGATTGAATTTATCCCGATGCAGGTGATCCCTATCGCCGGGATGATTGCCGGTAACGCCATGGTAGCGGTGGGGTTGTGTTACAACAATTTAGGGCAACGGGTCATTa       >  2:510565/1‑140 (MQ=255)
                                                                                                                            aCCCTGGCGGCGCTGATTCTCTCAGGGTCGATTGAATTTATCCCGATGCAGGTGATCCCTATCGCCGGGATGATTGCCGGTAACGCCATGGTAGCGGTGGGGTTGTGTTACAACAATTTAGGGCAACGGGTCATTAGCGa   >  2:174799/1‑140 (MQ=255)
                                                                                                                             cccTGGCGGCGCTGATTCTCTCAGGGTCGATTGAATTTATCCCGATGCAGGTGATCCCTATCGCCGGGATGATTGCCGGTAACGCCATGGTAGCGGTGGGGTTGTGTTACAACAATTTAGGGCAACGGGTCATTAGCGaa  <  2:175758/140‑1 (MQ=255)
                                                                                                                             cccTGGCGGCGCTGATTCTCTCAGGGTCGATTGAATTTATCCCGATGCAGGTGATCCCTATCGCCGGGATGATTGCCGGTAACGCCATGGTAGCGGTGGGGTTGTGTTACAACAATTTAGGGCAACGGGTCATTAGCGaa  <  1:522033/140‑1 (MQ=255)
                                                                                                                             cccTGGCGGCGCTGATTCTCTCAGGGTCGATTGAATTTATCCCGATGCAGGTGATCCCTATCGCCGGGATGATTGCCGGTAACGCCATGGTAGCGGTGGGGTTGTGTTACAACAATTTAGGGCAACGGGTCATTAGCGaa  <  1:174799/140‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                  
CCTGACATTATTGATGGTGTTATTTATCTGCTTTAATGCGGCGTGGAACGCGCAAAAACGCAGTAAATATATTGCTAAAGCTTTTATCTCATCGTTTATTGCCATTACGGTCGGGGCGGGAATTACCCTGGCGGTGCTGATTCTCTCAGGGTCGATTGAATTTATCCCGATGCAGGTGATCCCTATCGCCGGGATGATTGCCGGTAACGCCATGGTAGCGGTGGGGTTGTGTTACAACAATTTAGGGCAACGGGTCATTAGCGAA  >  NC_000913/516774‑517038

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑