Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	545,941	A→G	D141D (GAT→GAC)	allD ←	ureidoglycolate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	545,941	0	A	G	100.0%	168.9 / NA	47	D141D (GAT→GAC)	allD	ureidoglycolate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/26);  total (21/26)

TTCCCCATGCCTGTACGGTAGTCGCCATATCAAAGGTAAGGATCTCGTCGCCTTCTCCCGGCGCGGCAAAGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGATCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGACCCATCCGGCTGATACCGACCACCGCAACGCCATTTTGCTGGGCGGTTTTGATGGCA  >  NC_000913/545814‑546076                                                                                                                                |                                                                                                                                        ttCCCCATGCCTGTACGGTAGTCGCCATATCAAAGGTAAGGATCTCGTCGCCTTCTCCCGGCGCGGCAAAGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGcac                                                                                                                             >  2:656485/1‑140 (MQ=255)                  gTAGTCGCCATATCAAAGGTAAGGATCTCGTCGCCTTCTCCCGGCGCGGCAAAGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGaa                                                                                                            >  2:133987/1‑140 (MQ=255)                   tAGTCGCCATATCAAAGGTAAGGATCTCGTCGCCTTCTCCCGGCGCGGCAAAGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTTGGACTGGCACATCGAAATGCCAATGAAt                                                                                                           >  2:746908/1‑140 (MQ=255)                   tAGTCGCCATATCAAAGGTAAGGATCTCGTCGCCTTCTCCCGGCGCGGCAAAGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAAt                                                                                                           >  2:282063/1‑140 (MQ=255)                     gTCGCCATATCAAAGGTAAGGATCTCGTCGCCTTCTCCCGGCGCGGCAAAGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATcc                                                                                                         >  1:838185/1‑140 (MQ=255)                        gCCATATCAAAGGTAAGGATCTCGTCGCCTTCTCCCGGCGCGGCAAAGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCTAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGgc                                                                                                      >  2:182016/1‑140 (MQ=255)                             atCAAAGGTAAGGATCTCGTCGCCTTCTCCCGGCGCGGCAAAGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCggg                                                                                                 >  1:802601/1‑140 (MQ=255)                             atCAAAGGTAAGGATCTCGTCGCCTTCTCCCGGCGCGGCAAAGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCggg                                                                                                 >  1:473860/1‑140 (MQ=255)                                    gTAAGGATCTCGTCGCCTTCTCCCGGCGCGGCAAAGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGctgcctg                                                                                          <  1:26698/140‑1 (MQ=255)                                     tAAGGATCTCGTCGCCTTCTCCCGGCGCGGCAAAGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGctgcctgc                                                                                         <  1:133987/140‑1 (MQ=255)                                       aGGATCTCGTCGCCTTCTCCCGGCGCGGCAAAGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGgcgc                                                                                                    >  1:467126/1‑127 (MQ=255)                                       aGGATCTCGTCGCCTTCTCCCGGCGCGGCAAAGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGgcgc                                                                                                    <  2:467126/127‑1 (MQ=255)                                       aGGATCTCGTCGCCTTCTCCCGGCGCGGCAAAGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGgcgc                                                                                                    <  2:741485/127‑1 (MQ=255)                                       aGGATCTCGTCGCCTTCTCCCGGCGCGGCAAAGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGgcgc                                                                                                    >  1:741485/1‑127 (MQ=255)                                       aGGATCTCGTCGCCTTCTCCCGGCGCGGCAAAGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCctgctg                                                                                       <  2:326453/140‑1 (MQ=255)                                       aGGATCTCGTCGCCTTCTCCCGGCGCGGCAAAGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCctgctg                                                                                       <  1:746908/140‑1 (MQ=255)                                        ggATCTCGTCGCCTTCTCCCGGCGCGGCAAAGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCCtgctgc                                                                                      >  1:850686/1‑140 (MQ=255)                                         gATCTCGTCGCCTTCTCCCGGCGCGGAAAAGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCCTGCTGca                                                                                     <  2:603450/140‑1 (MQ=255)                                           tctcGTCGCCTTCTCCCGGCGCGGCAAAGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCCTGCTGcaca                                                                                   <  1:234090/140‑1 (MQ=255)                                            ctcGTCGCCTTCTCCCGGCGCGGCAAAGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCCTGCTGCACaa                                                                                  >  2:405921/1‑140 (MQ=255)                                              cgtcgCCTTCTCCCGGCGCGGCAAAGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGctgcctgc                                                                                         >  1:690944/1‑131 (MQ=255)                                              cgtcgCCTTCTCCCGGCGCGGCAAAGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGctgcctgc                                                                                         <  2:690944/131‑1 (MQ=255)                                               gtcgCCTTCTCCCGGCGCGGCAAAGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCggg                                                                                                 <  1:458901/122‑1 (MQ=255)                                               gtcgCCTTCTCCCGGCGCGGCAAAGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCggg                                                                                                 >  2:458901/1‑122 (MQ=255)                                                     ttCTCCCGGCGCGGCAAAGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAgaga                                                                         <  1:86926/140‑1 (MQ=255)                                                          ccGGCGCGGCAAAGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTgcg                                                                    <  2:347886/140‑1 (MQ=255)                                                          ccGGCGCGGCAAAGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTgcg                                                                    <  2:850686/140‑1 (MQ=255)                                                             gcgcGGCAAAGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTGcgccg                                                                 >  1:198326/1‑140 (MQ=255)                                                                    aaaGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGAc                                                          <  2:802601/140‑1 (MQ=255)                                                                    aaaGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGAc                                                          <  2:473860/140‑1 (MQ=255)                                                                        gCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCTAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGACCCAt                                                      <  1:182016/140‑1 (MQ=255)                                                                        gCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGACCCAt                                                      <  1:532609/140‑1 (MQ=255)                                                                             ggggTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGACCCATCCGGc                                                 <  2:198326/140‑1 (MQ=255)                                                                                  tAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGACCCATCCGGCTGATa                                            >  2:758741/1‑140 (MQ=255)                                                                                  tAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGACCCATCCGGCTGATa                                            <  1:282063/140‑1 (MQ=255)                                                                                       ccGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGTACATCGAAATGCCAATGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGACCCATCCGGCTGATACCGac                                       >  2:347160/1‑140 (MQ=255)                                                                                       ccGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGACCCATCCGGCTGATAcc                                          >  2:174646/1‑137 (MQ=255)                                                                                       ccGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGACCCATCCGGCTGATAcc                                          <  1:174646/137‑1 (MQ=255)                                                                                       ccGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGACCCATCCGGCTGATACCGac                                       <  1:656485/140‑1 (MQ=255)                                                                                         gtagtaAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGACCCATCCGGCTGATACCGacca                                     >  2:821657/1‑140 (MQ=255)                                                                                         gtagtaAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAACGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGACCCATCCGGCTGATACCGacca                                     >  1:137028/1‑140 (MQ=255)                                                                                                       cgccgcCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGACCCATCCGGCTGATACCGACCACCGCAACGCCAttt                       >  1:319912/1‑140 (MQ=255)                                                                                                               aaCGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGACCCATCCGGCTGATACCGACCACCGCAACGCCATTTTGCTGGGc               <  2:790894/140‑1 (MQ=255)                                                                                                                      ccaccaTTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGACCCATCCGGCTGATACCGACCACCGCAACGCCATTTTGCTGGGCGGTTTTg        <  1:244488/140‑1 (MQ=255)                                                                                                                      ccaccaTTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGACCCATCCGGCTGATACCGACCACCGCAACGCCATTTTGCTGGGCGGTTTTg        <  1:821657/140‑1 (MQ=255)                                                                                                                            ttGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGACCCATCCGGCTGATACCGACCACCGCAACGCCATTTTGCTGGGCGGTTTTGATGGCa  <  2:319912/140‑1 (MQ=255)                                                                                                                            ttGGGTCGGACTGGCACATCGAAATGCCAACGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGACCCATCCGGCTGATACCGACCACCGCAACGCCATTTTGCTGGGCGGTTTTGATGGCa  <  2:137028/140‑1 (MQ=255)                                                                                                                                |                                                                                                                                        TTCCCCATGCCTGTACGGTAGTCGCCATATCAAAGGTAAGGATCTCGTCGCCTTCTCCCGGCGCGGCAAAGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGATCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGACCCATCCGGCTGATACCGACCACCGCAACGCCATTTTGCTGGGCGGTTTTGATGGCA  >  NC_000913/545814‑546076

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑