Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	606,051	A→G	L50P (CTT→CCT)	ybdJ ←	DUF1158 domain‑containing protein YbdJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	606,051	0	A	G	100.0%	162.7 / NA	45	L50P (CTT→CCT)	ybdJ	DUF1158 domain‑containing protein YbdJ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/21);  total (24/21)

GCAAAGTATGCCGCGAAGTACGGCGATAATCGACGTTTAATCCGCCAGCGAGAACCAGCGTCGCCAGATAAAGCGCAGAACAAAATACTCAATAGCGCCCAGCACTAAAAACCACAGACAAAACAATAAAGTGTAAAGCTGACTAAGATCCATCAGATGGAACATGGTCACCAGTTTTTGTGCCAGCGCCAGCCCCAGTGCGGGGGCGGGCAGCAGCAGGCAAGAGAGGAAAGCCATCAGCAAAATGCCTGCTGCGGTGGTCAAGGTTTCTA  >  NC_000913/605915‑606186                                                                                                                                         |                                                                                                                                        gCAAAGTATGCCGCGAAGTACGGCGATAATCGACGTTTAATCCGCCAGCGAGAACCAGCGTCGCCAGATAAAGCGCAGAACAAAATACTCAATAGCGCCCAGCACTAAAAACCACAGACAAAACAATAAAGTGTAAGGCt                                                                                                                                      >  1:496302/1‑140 (MQ=255)      gTATGCCGCGAAGTACGGCGATAATCGACGTTTAATCCGCCAGCGAGAACCAGCGTCGCCAGATAAAGCGCAGAACAAAATACTCAATAGCGCCCAGCACTAAAAACCACAGACAAAACAATAAAGTGTAAGGCTGACTa                                                                                                                                 >  1:592597/1‑140 (MQ=255)      gTATGCCGCGAAGTACGGCGATAATCGACGTTTAATCCGCCAGCGAGAACCAGCGTCGCCAGATAAAGCGCAGAACAAAATACTCAATAGCGCCCAGCACTAAAAACCACAGACAAAACAATAAAGTGTAAGGCTGACTa                                                                                                                                 >  2:670175/1‑140 (MQ=255)          gCCGCGAAGTACGGCGATAATCGACGTTTAATCCGCCAGCGAGAACCAGCGTCGCCAGATAAAGCGCAGAACAAAATACTCAATAGCGCCCAGCACTAAAAACCACAGACAAAACAATAAAGTGTAAGGCTGACTAAGAt                                                                                                                             >  2:657518/1‑140 (MQ=255)              cgAAGTACGGCGATAATCGACGTTTAATCCGCCAGCGAGAACCAGCGTCGCCAGATAAAGCGCAGAACAAAATACTCAATAGCGCCCAGCACTAAAAACCACAGACAAAACAATAAAGTGTAAGGCTGACTAAGATCCAt                                                                                                                         <  2:14718/140‑1 (MQ=255)              cgAAGTACGGCGATAATCGACGTTTAATCCGCCAGCGAGAACCAGCGTCGCCAGATAAAGCGCAGAACAAAATACTCAATAGCGCCCAGCACTAAAAACCACAGACAAAACAATAAAGTGTAAGGCTGACTAAGATCCAt                                                                                                                         <  2:496302/140‑1 (MQ=255)               gAAGTACGGCGATAATCGACGTTTAATCCGCCAGCGAGAACCAGCGTCGCCAGATAAAGCGCAGAACAAAATACTCAATAGCGCCCAGCACTAAAAACCACAGACAAAACAATAAAGTGTAAGGCTGACTAAGATCCATc                                                                                                                        >  1:297755/1‑140 (MQ=255)                 aGTACGGCGATAATCGACGTTTAATCCGCCAGCGAGAACCAGCGTCGCCAGATAAAGCGCAGAACAAAATACTCAATAGCGCCCAGCACTAAAAACCACAGACAAAACAATAAAGTGTAAGGCTGACTAAGATCCATCAg                                                                                                                      <  1:657518/140‑1 (MQ=255)                  gTACGGCGATAATCGACGTTTAATCCGCCAGCGAGAACCAGCGTCGCCAGATAAAGCGCAGAACAAAATACTCAATAGCGCCCAGCACTAAAAACCACAGACAAAACAATAAAGTGTAAGGCTGACTAAGATCCATCAGa                                                                                                                     <  2:122287/140‑1 (MQ=255)                                     ttAATCCGCCAGCGAGAACCAGCGTCGCCAGATAAAGCGCAGAACAAAATACTCAATAGCGCCCAGCACTAAAAACCACAGACAAAACAATAAAGTGTAAGGCTGACTAAGATCCATCAGATGGAACATGGTCACCAGtt                                                                                                  >  1:167102/1‑140 (MQ=255)                                            gCCAGCGAGAACCAGCGTCGCCAGATAAAGCGCAGAACAAAATACTCAATAGCGCCCAGCACTAAAAACCACAGACAAAACAATAAAGTGTAAGGCTGACTAAGATCCATCAGATGGAACATGGTCACCAGTTTTTGTgc                                                                                           >  2:154089/1‑140 (MQ=255)                                              cAGCGAGAACCAGCGTCGCCAGATAAAGCGCAGAACAAAATACTCAATAGCGCCCAGCACTAAAAACCACAGACAAAACAATAAAGTGTAAGGCTGACTAAGATCCATCAGATGGAACATGGTCACCAGTTTTTGTgcca                                                                                         >  1:511867/1‑140 (MQ=255)                                                gCGAGAACCAGCGTCGCCAGATAAAGCGCAGAACAAAATACTCAATAGCGCCCAGCACTAAAAACCACAGACAAAACAATAAAGTGTAAGGCTGACTAAGATCCATCAGATGGAACATGGTCACCAGTTTTTGTgccagc                                                                                       >  1:355037/1‑140 (MQ=255)                                                 cGAGAACCAGCGTCGCTAGATAAAGCGCAGAACAAAATACTCAATAGCGCCCAGCACTAAAAACCACAGACAAAACAATAAAGTGTAAGGCTGACTAAGATCCATCAGATGGAACATGGTCACCAGTTTTTGTgccagcg                                                                                      >  1:719626/1‑140 (MQ=255)                                                        cAGCGTCGCCAGATAAAGCGCAGAACAAAATACTCAATAGCGCCCAGCACTAAAAACCACAGACAAAACAATAAAGTGTAAGGCTGACTAAGATCCATCAGATGGAACATGGTCACCAGTTTTTGTGCCAGCGCCAGccc                                                                               <  2:592597/140‑1 (MQ=255)                                                        cAGCGTCGCCAGATAAAGCGCAGAACAAAATACTCAATAGCGCCCAGCACTAAAAACCACAGACAAAACAATAAAGTGTAAGGCTGACTAAGATCCATCAGATGGAACATGGTCACCAGTTTTTGTGCCAGCGCCAGccc                                                                               <  2:355037/140‑1 (MQ=255)                                                        cAGCGTCGCCAGATAAAGCGCAGAACAAAATACTCAATAGCGCCCAGCACTAAAAACCACAGACAAAACAATAAAGTGTAAGGCTGACTAAGATCCATCAGATGGAACATGGTCACCAGTTTTTGTGCCAGCGCCAGccc                                                                               <  1:213658/140‑1 (MQ=255)                                                          gCGTCGCCAGATAAAGCGCAGAACAAAATACTCAATAGCGCCCAGCACTAAAAACCACAGACAAAACAATAAAGTGTAAGGCTGACTAAGATCCATCAGATGGAACATGGTCACCAGTTTTTGTGCCAGCGCCAGCCCCa                                                                             >  2:733109/1‑140 (MQ=255)                                                                        aGCGCAGAACAAAATACTCAATAGCGCCCAGCACTAAAAACCACAGACAAAACAATAAAGTGTAAGGCTGACTAAGATCCATCAGATGGAACATGGTCACCAGTTTTTGTGCCAGCGACAGCCCCAGTGCGGggacgggc                                                               >  2:226218/1‑140 (MQ=255)                                                                              gAACAAAATACTCAATAGCGCCCAGCACTAAAAACCACAGACAAAACAATAAAGTGTAAGGCTGACTAAGATCCATCAGATGGAACATGGTCACCAGTTTTTGTGCCAGCGCCAGCCCCAGTGCGGGGGCGGgcagcagc                                                         >  1:431078/1‑140 (MQ=255)                                                                              gAACAAAATACTCAATAGCGCCCAGCACTAAAAACCACAGACAAAACAATAAAGTGTAAGGCTGACTAAGATCCATCAGATGGAACATGGTCACCAGTTTTTGTGCCAGCGCCAGCCCCAGTGCGGGGGCGGgcagcagc                                                         >  1:245656/1‑140 (MQ=255)                                                                               aaCAAAATACTCAATAGCGCCCAGCACTAAAAACCACAGACAAAACAATAAAGTGTAAGGCTGACTAAGATCCATCAGATGGAACATGGTCACCAGTTTTTGTGCCAGCGCCAGCCCCAGTGCGGGGGCGGgcagcagca                                                        <  1:81625/140‑1 (MQ=255)                                                                                 cAAAATACTCAATAGCGCCCAGCACTAAAAACCACAGACAAAACAATAAAGTGTAAGGCTGACTAAGATCCATCAGATGGAACATGGTCACCAGTTTTTGTGCCAGCGCCAGCCCCAGTGCGGGGGCGGGCAGCAGCAgg                                                      <  1:234060/140‑1 (MQ=255)                                                                                     aTACTCAATAGCGCCCAGCACTAAAAACCACAGACAAAACAATAAAGTGTAAGGCTGACTAAGATCCATCAGATGGAACATGGTCACCAGTTTTTGTGCCAGCGCCAGCCCCAGTGCGGGGGCGGGCAGCAGCAGGCAag                                                  <  2:167102/140‑1 (MQ=255)                                                                                        cTCAATAGCGCCCAGCACTAAAAACCACAGACAAAACAATAAAGTGTAAGGCTGACTAAGATCCATCAGATGGAACATGGTCACCAGTTTTTGTGCCAGCGCCAGCCCCAGTGCGGGGGCGGGCAGCAGCAGGCAagaga                                               <  1:154089/140‑1 (MQ=255)                                                                                         tCAATAGCGCCCAGCACTAAAAACCACAGACAAAACAATAAAGTGTAAGGCTGACTAAGATCCATCAGATGGAACATGGTCACCAGTTTTTGTGCCAGCGCCAGCCCCAGTGCGGGGGCGGGCAGCAGCAGGCAagagag                                              >  1:113602/1‑140 (MQ=255)                                                                                         tCAATAGCGCCCAGCACTAAAAACCACAGACAAAACAATAAAGTGTAAGGCTGACTAAGATCCATCAGATGGAACATGGTCACCAG‑TTTTGTGCCAGCGCCAGCCCCAGTGCGGGGGCGGgcag                                                             >  2:99174/1‑124 (MQ=255)                                                                                         tCAATAGCGCCCAGCACTAAAAACCACAGACAAAACAATAAAGTGTAAGGCTGACTAAGATCCATCAGATGGAACATGGTCACCAG‑TTTTGTGCCAGCGCCAGCCCCAGTGCGGGGGCGGgcag                                                             <  1:99174/124‑1 (MQ=255)                                                                                          cAATAGCGCCCAGCACTAAAAACCACAGACAAAACAATAAAGTGTAAGGCTGACTAAGATCCATCAGATGGAACATGGTCACCAGTTTTTGTGCCAGCGCCAGCCCCAGTGCGGGGGCGGGCAGCAGCAGGCAAGAGAgg                                             <  2:511867/140‑1 (MQ=255)                                                                                           aaTAGCGCCCAGCACTAAAAACCACAGACAAAACAATAAAGTGTAAGGCTGACTAAGATCCATCAGATGGAACATGGTCACCAGTTTTTGTGCCAGCGCCAGCCCCAGTGCGGGGGCGGGCAGCAGCAGGCaa                                                   >  2:379239/1‑133 (MQ=255)                                                                                           aaTAGCGCCCAGCACTAAAAACCACAGACAAAACAATAAAGTGTAAGGCTGACTAAGATCCATCAGATGGAACATGGTCACCAGTTTTTGTGCCAGCGCCAGCCCCAGTGCGGGGGCGGGCAGCAGCAGGCaa                                                   <  1:379239/133‑1 (MQ=255)                                                                                              aGCGCCCAGCACTAAAAACCACAGACAAAACAATAAAGTGTAAGGCTGACTAAGATCCATCAGATGGAACATGGTCACCAGTTTTTGTGCCAGCGCCAGCCCCAGTGCGGGGGCGGGCAGCAGCAgg                                                      >  2:854355/1‑127 (MQ=255)                                                                                              aGCGCCCAGCACTAAAAACCACAGACAAAACAATAAAGTGTAAGGCTGACTAAGATCCATCAGATGGAACATGGTCACCAGTTTTTGTGCCAGCGCCAGCCCCAGTGCGGGGGCGGGCAGCAGCAgg                                                      <  1:854355/127‑1 (MQ=255)                                                                                                  cccAGCACTAAAAACCACAGACAAAACAATAAAGTGTAAGGCTGACTAAGATCCATCAGATGGAACATGGTCACCAGTTTTTGTGCCAGCGCTAGCCCCAGTGCGGGGGCGGGCAGCAGCAGGCAAGAGAGGAAAGCCAt                                     <  2:114177/140‑1 (MQ=255)                                                                                                       cACTAAAAACCACAGACAAAACAATAAAGTGTAAGGCTGACTAAGATCCATCAGATGGAACATGGTCACCAGTTTTTGTGCCAGCGCCAGCCCCAGTGCGGGGGCGGGCAGCAGCAGGCAAGAGAGGAAAGCCATCAGCa                                >  2:838188/1‑140 (MQ=255)                                                                                                                 cacaGACAAAACAATAAAGTGTAAGGCTGACTAAGATCCATCAGATGGAACATGGTCACCAGTTTTTGTGCCAGCGCCAGCCCCAGTGCGGGGGCGGGCAGCAGCAGGCAAGAGAGGAAAGCCATCAGCAAAATGCctgc                      >  1:445278/1‑140 (MQ=255)                                                                                                                  acaGACAAAACAATAAAGTGTAAGGCTGACTAAGATCCATCAGATGGAACATGGTCACCAGTTTTTGTGCCAGCGCCAGCCCCAGTGCGGGGGCGGGCAGCAGCAGGCAAGAGAGGAAAGCCATCAGCAAAATGCctgct                     >  2:683495/1‑140 (MQ=255)                                                                                                                    aGACAAAACAATAAAGTGTAAGGCTGACTAAGATCCATCAGATGGAACATGGTCACCAGTTTTTGTGCCAGCGCCAGCCCCAGTGCGGGGGCGGGCAGCAGCAGGCAAGAGAGGAAAGCCATCAGCAAAATGCCtgctgc                   >  1:403970/1‑140 (MQ=255)                                                                                                                    aGACAAAACAATAAAGTGTAAGGCTGACTAAGATCCATCAGATGGAACATGGTCACCAGTTTTTGTGCCAGCGCCAGCCCCAGTGCGGGGGCGGGCAGCAGCAGGCAAGAGAGGAAAGCCATCAGCAAAATGCCtgctgc                   >  1:345708/1‑140 (MQ=255)                                                                                                                     gACAAAACAATAAAGTGTAAGGCTGACTAAGATCCATCAGATGGAACATGGTCACCAGTTTTTGTGCCAGCGCCAGCCCCAGTGCGGGGGCGGGCAGCAGCAGGCAAGAGAGGAAAGCCATCAGCAAAATGCCTGCTGCg                  <  2:719626/140‑1 (MQ=255)                                                                                                                           aCAATAAAGTGTAAGGCTGACTAAGATCCATCAGATGGAACATGGTCACCAGTTTTTGTGCCAGCGCCAGCCCCAGTGCGGGGGCGGGCAGCAGCAGGCAAGAGAGGAAAGCCATCAGCAAAATGCCTGCTGCGGTGGTc            <  2:687140/140‑1 (MQ=255)                                                                                                                           aCAATAAAGTGTAAGGCTGACTAAGATCCATCAGATGGAACATGGTCACCAGTTTTTGTGCCAGCGCCAGCCCCAGTGCGGGGGCGGGCAGCAGCAGGCAAGAGAGGAAAGCCATCAGCAAAATGCCTGCTGCGGTGGTc            <  1:838188/140‑1 (MQ=255)                                                                                                                               tAAAGTGTAAGGCTGACTAAGATCCATCAGATGGAACATGGTCACCAGTTTTTGTGCCAGCGCCAGCCCCAGTGCGGGGGCGGGCAGCAGCAGGCAAGAGAGGAAAGCCATCAGCAAAATGCCTGCTGCGGTGGTCAAgg        <  1:683495/140‑1 (MQ=255)                                                                                                                                    tgtAAGGCTGACTAAGATCCATCAGATGGAACATGGTCACCAGTTTTTGTGCCACCGCCAGCCCCAGTGCGGGGGCGGGCAGCACCAGGCAAGAGAGGAAAGCCATCAGCAAAATGCCTGCTGCGGCGGTCAAGGTTTCt   >  2:323275/1‑140 (MQ=255)                                                                                                                                     gtAAGGCTGACTAAGATCCATCAGATGGAACATGGTCACCAGTTTTTGTGCCAGCGCCAGCCCCAGTGCGGGGGCGGGCAGCAGCAGGCAAGAGAGGAAAGCCATCAGCAAAATGCCTGCTGCGGTGGTCAAGGTTTCTa  <  2:403970/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        GCAAAGTATGCCGCGAAGTACGGCGATAATCGACGTTTAATCCGCCAGCGAGAACCAGCGTCGCCAGATAAAGCGCAGAACAAAATACTCAATAGCGCCCAGCACTAAAAACCACAGACAAAACAATAAAGTGTAAAGCTGACTAAGATCCATCAGATGGAACATGGTCACCAGTTTTTGTGCCAGCGCCAGCCCCAGTGCGGGGGCGGGCAGCAGCAGGCAAGAGAGGAAAGCCATCAGCAAAATGCCTGCTGCGGTGGTCAAGGTTTCTA  >  NC_000913/605915‑606186

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑