Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	46,123	C→T	P106L (CCC→CTC)	yaaU →	putative transporter YaaU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	46,123	0	C	T	100.0%	211.7 / NA	60	P106L (CCC→CTC)	yaaU	putative transporter YaaU
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/26);  total (34/26)

GAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATTGCCGCGATGTG  >  NC_000913/45988‑46258                                                                                                                                        |                                                                                                                                        gAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCTCGTc                                                                                                                                     >  2:655647/1‑140 (MQ=255)  aaCGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCtcgtcg                                                                                                                                    <  2:546126/140‑1 (MQ=255)    cGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCTCGTCGaa                                                                                                                                  <  2:15156/140‑1 (MQ=255)      cTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCTCGTCGAACt                                                                                                                                >  1:585071/1‑140 (MQ=255)            gggCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCTCGTCGAACTGTTGGt                                                                                                                          <  2:611624/140‑1 (MQ=255)               cTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCTCGTCGAACTGTTGGTGAt                                                                                                                       <  2:519636/140‑1 (MQ=255)                   tCGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCTCGTCGAAc                                                                                                                                 >  1:578114/1‑126 (MQ=255)                   tCGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCTCGTCGAAc                                                                                                                                 <  2:578114/126‑1 (MQ=255)                   tCGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCTCGTCGAACTGTTGGTGAt                                                                                                                       <  2:727192/136‑1 (MQ=255)                   tCGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCTCGTCGAACTGTTGGTGAt                                                                                                                       >  1:727192/1‑136 (MQ=255)                   tCGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCTCGTCGAACTGTTGGTGATGCgg                                                                                                                   >  1:640710/1‑140 (MQ=255)                              tCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCTCGTCGAACTGTTGGTGATGCGGGTACTTATCgg                                                                                                        >  2:200207/1‑140 (MQ=255)                               cGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCTCGTCGAACTGTTGGTGATGCGGGTACTTATCGGc                                                                                                       >  2:210171/1‑140 (MQ=255)                                      tGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCTCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCa                                                                                                <  2:207657/140‑1 (MQ=255)                                       ggTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCTCGTCGAACTGTTGGTGATGCGGGTa                                                                                                                >  2:520086/1‑123 (MQ=255)                                       ggTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCTCGTCGAACTGTTGGTGATGCGGGTa                                                                                                                <  1:520086/123‑1 (MQ=255)                                       ggTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCTCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCAt                                                                                               <  1:210171/140‑1 (MQ=255)                                       ggTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCTCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCAt                                                                                               <  2:656979/140‑1 (MQ=255)                                         ttATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCTCGTCGAACTGTTGGTGATGCGGGTACTTAt                                                                                                           >  2:200309/1‑126 (MQ=255)                                         ttATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCTCGTCGAACTGTTGGTGATGCGGGTACTTAt                                                                                                           <  1:200309/126‑1 (MQ=255)                                         ttATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCTCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCa                                                                                                      >  1:81031/1‑131 (MQ=255)                                         ttATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCTCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCa                                                                                                      <  2:81031/131‑1 (MQ=255)                                         ttATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCTCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCg                                                                                             >  2:523306/1‑140 (MQ=255)                                         ttATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCTCGTCGAACTGTTGGTGATGCGGGTACTTAACGGc                                                                                                       >  2:192980/1‑130 (MQ=255)                                         ttATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCTCGTCGAACTGTTGGTGATGCGGGTACTTAACGGc                                                                                                       <  1:192980/130‑1 (MQ=255)                                              tttCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCTCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCAt                                                                                               >  1:462860/1‑133 (MQ=255)                                              tttCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCTCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCAt                                                                                               <  2:462860/133‑1 (MQ=255)                                              tttCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCTCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCa                                                                                        >  2:226548/1‑140 (MQ=255)                                              tttCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCTCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCa                                                                                        >  1:338404/1‑140 (MQ=255)                                                  cGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCTCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGAtt                                                                                    >  1:292099/1‑140 (MQ=255)                                                  cGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCTCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGAtt                                                                                    <  2:292099/140‑1 (MQ=255)                                                  cGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCTCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGAtt                                                                                    >  1:403702/1‑140 (MQ=255)                                                  cGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCTCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGAtt                                                                                    >  1:132149/1‑140 (MQ=255)                                                   gATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCTCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTa                                                                                   >  1:582707/1‑140 (MQ=255)                                                    aTAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCTCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTAt                                                                                  >  2:743201/1‑140 (MQ=255)                                                         gTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCTCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCAt                                                                             <  2:585071/140‑1 (MQ=255)                                                                  cgcAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCTCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCa                                                                        <  1:464646/136‑1 (MQ=255)                                                                  cgcAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCTCGTCAAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCa                                                                        >  2:464646/1‑136 (MQ=255)                                                                    cAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCTCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCaa                                                                  <  2:338404/140‑1 (MQ=255)                                                                            tCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCTCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCAcc                                                          >  1:299862/1‑140 (MQ=255)                                                                               tCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCTCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAg                                                       <  2:132149/140‑1 (MQ=255)                                                                               tCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCTCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAg                                                       <  1:655647/140‑1 (MQ=255)                                                                                cATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCTCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGt                                                      <  2:403702/140‑1 (MQ=255)                                                                                  ttGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCTCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTtc                                                    <  2:299862/140‑1 (MQ=255)                                                                                   tGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCTCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTtct                                                   >  2:313113/1‑140 (MQ=255)                                                                                     atatCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCTCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTcc                                                 >  1:742966/1‑140 (MQ=255)                                                                                      tatCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCTCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCa                                                <  1:200207/140‑1 (MQ=255)                                                                                          atcGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCTCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTAc                                            >  2:548175/1‑140 (MQ=255)                                                                                           tcGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCTCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTAcc                                           >  1:773806/1‑140 (MQ=255)                                                                                            cGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCTCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTAccc                                          >  2:467860/1‑140 (MQ=255)                                                                                             gCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCTCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCg                                         >  1:785036/1‑140 (MQ=255)                                                                                                   ggCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCTCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCg                                   <  2:785036/140‑1 (MQ=255)                                                                                                             cGGTGGCGACGATGTTTGTTTCATCCCTCGTCGAACTGTTGGTGATGCGGGTACTTATCTGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTcc                         >  2:291677/1‑140 (MQ=255)                                                                                                                   cgacgaTGTTTGTTTCATCCCTCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGc                   >  2:680405/1‑140 (MQ=255)                                                                                                                            ttgtttCATCCCTCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATTGcc          >  2:315100/1‑140 (MQ=255)                                                                                                                              gtttCATCCCTCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATTGCcgc        <  1:363472/140‑1 (MQ=255)                                                                                                                                  cATCCCTCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATTGCCGCGAtg    <  1:467860/140‑1 (MQ=255)                                                                                                                                   aTCCCTCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATTGCcgcg       >  1:44832/1‑136 (MQ=255)                                                                                                                                   aTCCCTCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATTGCcgcg       <  2:44832/136‑1 (MQ=255)                                                                                                                                   aTCCCTCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATTGCCGCGAtgt   >  2:29860/1‑140 (MQ=255)                                                                                                                                   aTCCCTCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATTGCCGCGAtgt   >  2:264075/1‑140 (MQ=255)                                                                                                                                    tCCCTCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATTGCCGCGAtgtg  >  1:625220/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                        GAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATTGCCGCGATGTG  >  NC_000913/45988‑46258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑