Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,066,280	A→G	V232V (GTA→GTG)	agp →	glucose‑1‑phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,066,280	0	A	G	100.0%	120.9 / NA	35	V232V (GTA→GTG)	agp	glucose‑1‑phosphatase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/14);  total (21/14)

CTATAAAGATTCCCCTGCCTGTAAAGAGAAACAACAGTGTTCGCTGGTGGATGGCAAAAATACCTTTAGCGCCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTAGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCAGGTGGCCTGGGGAGAAATCAAATCTGACCAGCAGTGGAAGGTGTTGTCGAAGCTTAAAAACGGCTACCAGGACAGC  >  NC_000913/1066151‑1066400                                                                                                                                  |                                                                                                                         cTATAAAGATTCCCCTGCCTGTAAAGAGAAACAACAGTGTTCGCTGGTGGATGGCAAAAATACCTTTAGCGCCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTGGATGCGTTTa                                                                                                                <  1:108725/140‑1 (MQ=255)            ccccTGCCTGTAAAGAGAAACAACAGTGTTCGCTGGTGGATGGCAAAAATACCTTTAGCGCCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTGGATGCGTTTACTTTGCAatat                                                                                                     >  2:370839/1‑140 (MQ=255)                tgcctgTAAAGAGAAACAACAGTGTTCGCTGGTGGATGGCAAAAATACCTTTAGCGCCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTGGATGCGTTTACTTTGCAATATTACg                                                                                                 >  1:129963/1‑140 (MQ=255)                  cctgTAAAGAGAAACAACAGTGTTCGCTGGTGGATGGCAAAAATACCTTTAGCGCCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTGGATGCGTTTACTTTGCAATATTACGaa                                                                                               >  1:241326/1‑140 (MQ=255)                       aaaGAGAAACAACAGTGTTCGCTGGTGGATGGCAAAAATACCTTTAGCGCCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTGGATGCGTTTACTTTGCAATATTACGAAGGttt                                                                                          <  2:241326/140‑1 (MQ=255)                            gaAACAACAGTGTTCGCTGGTGGATGGCAAAAATACCTTTAGCGCCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTGGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGa                                                                                     >  2:141594/1‑140 (MQ=255)                            gaAACAACAGTGTTCGCTGGTGGATGGCAAAAATACCTTTAGCGCCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTGGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGa                                                                                     >  1:352929/1‑140 (MQ=255)                             aaaCAACAGTGTTCGCTGGTGGATGGCAAAAATACCTTTAGCGCCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTGGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGAt                                                                                    >  1:140861/1‑140 (MQ=255)                              aacaacAGTGTTCGCTGGTGGATGGCAAAAATACCTTTAGCGCCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTGGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATg                                                                                   >  1:328192/1‑140 (MQ=255)                               acaacaGTGTTCGCTGGTGGATGGCAAAAATACCTTTAGCGCCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTGGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATgg                                                                                  >  1:494893/1‑140 (MQ=255)                               acaacaGTGTTCGCTGGTGGATGGCAAAAATACCTTTAGCGCCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTGGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATgg                                                                                  <  2:328987/140‑1 (MQ=255)                                    aGTGTTCGCTGGTGGATGGCAAAAATACCTTTAGCGCCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTGGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCAg                                                                             <  2:352929/140‑1 (MQ=255)                                     gtgtTCGCTGGTGGATGGCAAAAATACCTTTAGCGCCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTGGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCAgg                                                                            <  1:141594/140‑1 (MQ=255)                                     gtgtTCGCTGGTGGATGGCAAAAATACCTTTAGCGCCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCAGCAACTCGCTGGTGGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCAgg                                                                            >  1:26739/1‑140 (MQ=255)                                        ttCGCTGGTGGATGGCAAAAATACCTTTAGCGCCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTGGATGCGTTTACTTTGCAATATTACGAAGGttt                                                                                          <  1:297975/123‑1 (MQ=255)                                        ttCGCTGGTGGATGGCAAAAATACCTTTAGCGCCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTGGATGCGTTTACTTTGCAATATTACGAAGGttt                                                                                          >  2:297975/1‑123 (MQ=255)                                           gCTGGTGGATGGCAAAAATACCTTTAGCGCCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTGGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCAGGTGGCCt                                                                      >  2:853731/1‑140 (MQ=255)                                            cTGGTGGATGGCAAAAATACCTTTAGCGCCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTGGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCAGGTGGCCTg                                                                     <  2:129963/140‑1 (MQ=255)                                             tggtggATGGCAAAAATACCTTTAGCGCCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTGGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCAGGTGGCCTgg                                                                    >  2:567028/1‑140 (MQ=255)                                                tggATGGCAAAAATACCTTTAGCGCCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTGGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCAGGTGGCCTTGGga                                                                 <  2:140861/140‑1 (MQ=255)                                                tggATGGCAAAAATACCTTTAGCGCCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTGGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCAGGTGGCCTGGGga                                                                 >  1:282132/1‑140 (MQ=255)                                                    tGGCAAAAATACCTTTAGCGCCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTGGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCAGGTGGCCTGGGGAGaaa                                                             >  2:545712/1‑140 (MQ=255)                                                     ggCAAAAATACCTTTAGCGCCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTGGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCAGGTGGCCTGGGGAGaaat                                                            <  2:282132/140‑1 (MQ=255)                                                     ggCAAAAATACCTTTAGCGCCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTGGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCAGGTGGCCTGGGGAGaaat                                                            <  1:567028/140‑1 (MQ=255)                                                               ccTTTAGCGCCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTGGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCAGGTGGCCTGGGGAGAAATCAAATCTGAc                                                  <  2:328192/140‑1 (MQ=255)                                                                      cgcCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTGGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCAGGTGGCCTGGGGAGAAATCAAATCTGACCAGCAGt                                           >  2:460588/1‑140 (MQ=255)                                                                           aGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTGGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCAGGTGGCCTGGGGAGAAATCAAATCTGACCAGCAGTGGAAg                                      <  1:460588/140‑1 (MQ=255)                                                                                       aaCCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTGGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCAGGTGGCCTGGGGAGAAATCAAATCTGACCAGCAGTGGAAGGTGTTGTCGAAg                          >  2:214772/1‑140 (MQ=255)                                                                                       aaCCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTGGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCAGGTGGCCTGGGGAGAAATCAAATCTGACCAGCAGTGGAAGGTGTTGTCGAAg                          >  2:812914/1‑140 (MQ=255)                                                                                       aaCCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTGGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCAGGTGGCCTGGGGAGAAATCAAATCTGACCAGCAGTGGAAGGTGTTGTCGAAg                          >  2:734024/1‑140 (MQ=255)                                                                                            ggTGTTTCCGGGCCGCTGAAAGCCGGCAACTCGCTGGTGGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCAGGTGGCCTGGGGAGAAATCAAATCTGACCAGCAGTAGAAGGTGTTGTCGAAGCTTaa                     >  1:851204/1‑140 (MQ=255)                                                                                                 ttCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTGGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCAGGTGGCCTGGGGAGAAATCAAATCTGACCAGCAGTGGAAGGTGTTGTCGAAGCTTAAAAACg                >  2:283775/1‑140 (MQ=255)                                                                                                           cTGAAAGTCGGCAACTCGCTGGTGGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCAGGTGGCCTGGGGAGAAATCAAATCTGACCAGCAGTGGAAGGTGTTGTCGAAGCTTAAAAACGGCTACCAGGa      >  1:226014/1‑140 (MQ=255)                                                                                                            tGAAAGTCGGCAACTCGCTGGTGGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCAGGTGGCCTGGGGAGAAATCAAATCTGACCAGCAGTGGAAGGTGTTGTCGAAGCTTAAAAACGGCTACCAGGAc     <  1:812914/140‑1 (MQ=255)                                                                                                               aaGTCGGCAACTCGCTGGTGGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCAGGTGGCCTGGGGAGAAATCAAATCTGACCAGCAGTGGAAGGTGTTGTCGAAGCTTAAAAACGGCTACCAGGACAGc  <  1:545712/140‑1 (MQ=255)                                                                                                                                  |                                                                                                                         CTATAAAGATTCCCCTGCCTGTAAAGAGAAACAACAGTGTTCGCTGGTGGATGGCAAAAATACCTTTAGCGCCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTAGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCAGGTGGCCTGGGGAGAAATCAAATCTGACCAGCAGTGGAAGGTGTTGTCGAAGCTTAAAAACGGCTACCAGGACAGC  >  NC_000913/1066151‑1066400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑