Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,088,526	T→C	A444A (GCA→GCG)	pgaB ←	poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine N‑deacetylase and beta‑1,6 glycoside hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,088,526	0	T	C	100.0%	214.3 / NA	58	A444A (GCA→GCG)	pgaB	poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine N‑deacetylase and beta‑1,6 glycoside hydrolase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/28);  total (30/28)

TAAAGCCTGCTTGCTGATAAGCCGTGATAGCCGGTGCACTGGCATCTTCATAATCTGAAAGCAAAGCATCATCGTGGAACAATATGCCATCAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTAATACCGGCATCCAC  >  NC_000913/1088390‑1088661                                                                                                                                         |                                                                                                                                        tAAAGCCTGCTTGCTGATAAGCCGTGATAGCCGGTGCACTGGCATCTTCATAATCTGAAAGCAAAGCATCATCGTGGAACAATATGCCATCAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGct                                                                                                                                      <  2:755492/140‑1 (MQ=255)  aaaGCCTGCTTGCTGATAAGCCGTGATAGCCGGTGCACTGGCATCTTCATAATCTGAAAGCAAAGCATCATCGTGGAACAATATGCCATCAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGctc                                                                                                                                     <  2:177713/140‑1 (MQ=255)       cTGCTTGCTGATAAGCCGTGATAGCCGGTGCACTGGCATCTTCATAATCTGAAAGCAAAGCATCATCGTGGAACAATATGCCATCAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGAct                                                                                                                                <  2:563669/140‑1 (MQ=255)          cttgctGATAAGCCGTGATAGCCGGTGCACTGGCATCTTCATAATCTGAAAGCAAAGCATCATCGTGGAACAATATGCCATCAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGACTCTg                                                                                                                             <  1:342928/140‑1 (MQ=255)            tgctGATAAGCCGTGATAGCCGGTGCACTGGCATCTTCATAATCTGAAAGCAAAGCATCATCGTGGAACAATATGCCATCAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTc                                                                                                                           <  2:255425/140‑1 (MQ=255)            tgctGATAAGCCGTGATAGCCGGTGCACTGGCATCTTCATAATCTGAAAGCAAAGCATCATCGTGGAACAATATGCCATCAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTc                                                                                                                           <  1:511097/140‑1 (MQ=255)                  caggCCGTGATAGCCGGTGCACTGGCATCTTCATAATCTGAAAGCAAAGGATCATCGTGGAACAATATGCCATCAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGaa                                                                                                                     <  1:525380/137‑1 (MQ=255)                         tGATAGCCGGTGCACTGGCATCTTCATAATCTGAAAGCAAAGCATCATCGTGGAACAATATGCCATCAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGgagag                                                                                                              >  2:681057/1‑140 (MQ=255)                                ccgcgCACTGGCATCTTCATAATCTGAAAGCAAAGCATAATCGCGGCACAATATGCCATCAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCGAATTGCGATCTGACTCTGTCATCGAAAGAAGAGAGAcctg                                                                                                       >  2:525380/5‑137 (MQ=255)                                 ggTGCACTGGCATCTTCATAATCTGAAAGCAAAGCATCATCGTGGAACAATATGCCATCAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTg                                                                                                      >  2:322020/1‑140 (MQ=255)                                   tGCACTGGCATCTTCATAATCTGAAAGCAAAGCATCATCGTGGAACAATATGCCATCAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACgg                                                                                                        >  1:243338/1‑136 (MQ=255)                                   tGCACTGGCATCTTCATAATCTGAAAGCAAAGCATCATCGTGGAACAATATGCCATCAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACgg                                                                                                        <  2:243338/136‑1 (MQ=255)                                         ggCATCTTCATAATCTGAAAGCAAAGCATCATCGTGGAACAATATGCCATCAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCATCTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGtt                                                                                              <  2:100803/140‑1 (MQ=255)                                         ggCATCTTCATAATCTGAAAGCAAAGCATCATCGTGGAACAATATGCCATCAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGtt                                                                                              <  2:194788/140‑1 (MQ=255)                                          gCATCTTCATAATCTGAAAGCAAAGCATCATCGTGGAACAATATGCCATCAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTc                                                                                             >  2:106774/1‑140 (MQ=255)                                                    aaTCTGAAAGCAAAGCATCATCGTGGAACAATATGCCATCAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAAtt                                                                                   >  2:346390/1‑140 (MQ=255)                                                    aaTCTGAAAGCAAAGCATCATCGTGGAACAATATGCCATCAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAAtt                                                                                   >  1:217809/1‑140 (MQ=255)                                                          aaagcaaagcATCATCGTGGAACAATATGCCATCAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCt                                                                             >  1:632050/1‑140 (MQ=255)                                                          aaagcaaagcATCATCGTGGAACAATATGCCATCAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCt                                                                             >  1:343661/1‑140 (MQ=255)                                                          aaagcaaagcATCATCGTGGAACAATATGCCATCAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCt                                                                             <  2:632050/140‑1 (MQ=255)                                                           aagcaaagcaTCATCGTGGAACAATATGCCATCAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCtt                                                                            >  1:2979/1‑140 (MQ=255)                                                                 agcaTCATCGTGGAACAATATGCCATCAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTtct                                                                      <  2:469316/140‑1 (MQ=255)                                                                      catcGTGGAACAATATGCCATCAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCt                                                                 >  1:657261/1‑140 (MQ=255)                                                                       atcGTGGAACAATATGCCATCAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTg                                                                >  1:90879/1‑140 (MQ=255)                                                                             gAACAATATGCCATCAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTa                                                          >  2:1707/1‑140 (MQ=255)                                                                             gAACAATATGCCATCAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTa                                                          <  2:90879/140‑1 (MQ=255)                                                                             gAACAATATGCCATCAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTa                                                          >  2:578064/1‑140 (MQ=255)                                                                                   tatGCCATCAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTAtt                                                    >  2:618201/1‑140 (MQ=255)                                                                                       ccATCAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTAc                                                <  1:578064/140‑1 (MQ=255)                                                                                        cATCAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACt                                               >  1:731180/1‑140 (MQ=255)                                                                                          tCAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGTCGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTcg                                             >  2:559173/1‑140 (MQ=255)                                                                                          tCAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTAtttt                                                  >  1:273827/1‑135 (MQ=255)                                                                                          tCAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTAtttt                                                  <  2:273827/135‑1 (MQ=255)                                                                                          tCAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTcg                                             >  2:641859/1‑140 (MQ=255)                                                                                           cAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTcgc                                            >  1:151368/1‑140 (MQ=255)                                                                                            aaaaGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTcgcg                                           <  2:151368/140‑1 (MQ=255)                                                                                            aaaaGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTcgcg                                           <  1:681057/140‑1 (MQ=255)                                                                                                 cagcaTGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAAt                                      >  2:276530/1‑140 (MQ=255)                                                                                                  agcaTGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAAtg                                     <  1:276530/140‑1 (MQ=255)                                                                                                   gcaTGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAAtgt                                    <  1:340697/140‑1 (MQ=255)                                                                                                     aTGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTgg                                  <  2:657261/140‑1 (MQ=255)                                                                                                           ggCAAGATCTTCATATAACATGCTAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTa                            <  1:618201/140‑1 (MQ=255)                                                                                                                gATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATcc                       <  1:641859/140‑1 (MQ=255)                                                                                                                gATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCACGTTAATGTGGGATCTAAATcc                       <  2:217809/140‑1 (MQ=255)                                                                                                                  tCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATcc                       >  1:769820/1‑138 (MQ=255)                                                                                                                  tCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATcc                       <  2:769820/138‑1 (MQ=255)                                                                                                                  tCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCa                     >  2:502692/1‑140 (MQ=255)                                                                                                                         ataACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTAAt              >  1:513273/1‑140 (MQ=255)                                                                                                                           aaCATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTAATAc            >  2:152064/1‑140 (MQ=255)                                                                                                                             cATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTAATACCg          >  1:699389/1‑140 (MQ=255)                                                                                                                              aTGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTAATACCgg         <  1:152064/140‑1 (MQ=255)                                                                                                                                gCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTAATACCGGCa       >  2:369293/1‑140 (MQ=255)                                                                                                                                gCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTAATACCGGCa       >  2:609213/1‑140 (MQ=255)                                                                                                                                gCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTAATACCGGCa       >  1:709323/1‑140 (MQ=255)                                                                                                                                gCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTAATACCGGCa       >  2:681261/1‑140 (MQ=255)                                                                                                                                 ccAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTGAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTAATACCGGCAt      <  1:106774/140‑1 (MQ=255)                                                                                                                                  cAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTAATACCGGCATc     <  1:611822/140‑1 (MQ=255)                                                                                                                                   aaCTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGTCGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTAATACCGGCATcc    <  1:559173/140‑1 (MQ=255)                                                                                                                                     cTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTAATACCGGCATCCAc  >  1:858516/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        TAAAGCCTGCTTGCTGATAAGCCGTGATAGCCGGTGCACTGGCATCTTCATAATCTGAAAGCAAAGCATCATCGTGGAACAATATGCCATCAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTAATACCGGCATCCAC  >  NC_000913/1088390‑1088661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑