Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,112,537	T→C	W559R (TGG→CGG)	opgH →	osmoregulated periplasmic glucans (OPGs) biosynthesis protein H

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,112,537	0	T	C	100.0%	193.2 / NA	53	W559R (TGG→CGG)	opgH	osmoregulated periplasmic glucans (OPGs) biosynthesis protein H
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/28);  total (25/28)

TTCCTGACGGGCGTGATGTCTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTGGCGCGTTAC  >  NC_000913/1112402‑1112673                                                                                                                                        |                                                                                                                                         ttCCTGACGGGCGTGATGTCTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTT‑CTGCAACCACGGCAGTTGTTCCCAGTGCGGCCg                                                                                                                                     <  1:531005/140‑1 (MQ=255)  tCCTGACGGGCGTGATGTCTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGCGGCCg                                                                                                                                     <  1:820045/140‑1 (MQ=255)  tCCTGACGGGCGTGATGTCTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGCGGCCg                                                                                                                                     <  1:412474/140‑1 (MQ=255)    cTGACGGGCGTGATGTCTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGCGGCCGCa                                                                                                                                   <  1:162344/140‑1 (MQ=255)       aCGGGCGTGATGTCTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGCGGCCGCAGTg                                                                                                                                <  1:490468/140‑1 (MQ=255)         gggCGTGATGTCTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGCGGCCGCAGTGGc                                                                                                                              >  2:415509/1‑140 (MQ=255)            cGTGATGTCTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCTGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGCGGCCGCAGTGGCGTc                                                                                                                           >  2:817125/1‑140 (MQ=255)            cGTGATGTCTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGCGGCCGCAGTGGCGTc                                                                                                                           >  1:577156/1‑140 (MQ=255)               gATGTCTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGCGGCCGCAGTGGCGTCCTg                                                                                                                        <  1:257361/140‑1 (MQ=255)                         ctctcCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGCGGCCGCAGTGGCGTCCTGAGCTGGCGAt                                                                                                              <  2:190623/140‑1 (MQ=255)                             ccgctccgctGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGCGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCa                                                                                                          >  2:671024/1‑140 (MQ=255)                                      tgtgGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGCGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCt                                                                                                 <  2:790336/140‑1 (MQ=255)                                      tgtgGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGCGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCt                                                                                                 <  2:634510/140‑1 (MQ=255)                                        tgGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGCGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTc                                                                                               <  2:503714/140‑1 (MQ=255)                                             tATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGCGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCa                                                                                          <  1:502906/140‑1 (MQ=255)                                                gTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGCGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATgg                                                                                       <  1:671024/140‑1 (MQ=255)                                                   ccTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGCGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGc                                                                                    >  1:95237/1‑140 (MQ=255)                                                          ctctctACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGCGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTgttgtt                                                                             <  2:767138/140‑1 (MQ=255)                                                               tACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGCGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGc                                                                        <  2:577156/140‑1 (MQ=255)                                                                    cATTGCTGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGCGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAg                                                                   <  1:817125/140‑1 (MQ=255)                                                                       tGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGCGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGtaa                                                                >  1:22147/1‑138 (MQ=255)                                                                       tGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGCGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTa                                                                >  1:681335/1‑140 (MQ=255)                                                                         cAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGCGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTAtt                                                              >  1:861137/1‑140 (MQ=255)                                                                         cAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGCGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTAtt                                                              >  1:255396/1‑140 (MQ=255)                                                                         cAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGCGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTAtt                                                              >  1:42634/1‑140 (MQ=255)                                                                           ggTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGCGGCCGCAGTGGCGTCCTGAGCTGGCGGTTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGa                                                            >  2:790040/1‑140 (MQ=255)                                                                           ggTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGCGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGa                                                            >  1:723763/1‑140 (MQ=255)                                                                              aGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGCGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCa                                                         <  2:441064/140‑1 (MQ=255)                                                                               gTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGCGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCAt                                                        <  1:51263/140‑1 (MQ=255)                                                                               gTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGCGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCAt                                                        <  2:243885/140‑1 (MQ=255)                                                                                tGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGCGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCa                                                         <  1:432459/138‑1 (MQ=255)                                                                                tGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGCGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCa                                                         >  2:432459/1‑138 (MQ=255)                                                                                tGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGCGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCAtt                                                       >  2:121586/1‑140 (MQ=255)                                                                                 gCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGCGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCTTGCCGAAGTTATTGAGCAttt                                                      >  1:110483/1‑140 (MQ=255)                                                                                   aTGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGCGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCAt                                                        <  2:59074/136‑1 (MQ=255)                                                                                   aTGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGCGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCAt                                                        >  1:59074/1‑136 (MQ=255)                                                                                         tGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGCGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATc                                              <  2:257104/140‑1 (MQ=255)                                                                                          gACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGCGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCt                                             >  2:201864/1‑140 (MQ=255)                                                                                                aCCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGCGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCa                                                         >  2:538558/1‑122 (MQ=255)                                                                                                aCCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGCGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCa                                                         <  1:538558/122‑1 (MQ=255)                                                                                                aCCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGCGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCa                                       <  2:681335/140‑1 (MQ=255)                                                                                                     aaTACTTCCTGCAACCACGGCAGTTGTTCCCAGTGCGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGa                                  >  2:202313/1‑140 (MQ=255)                                                                                                      aTACTTCCTGCAACCACGGCAGTTGTTCCCAGTGCGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGaa                                 <  1:121586/140‑1 (MQ=255)                                                                                                      aTACTTCCTGCAACCACGGCAGTTGTTCCCAGTGCGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGaa                                 <  2:255396/140‑1 (MQ=255)                                                                                                      aTACTTCCTGCAACCACGGCAGTTGTTCCCAGTGCGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGaa                                 <  2:110483/140‑1 (MQ=255)                                                                                                       tACTTCCTGCAACCACGGCAGTTGTTCCCAGTGCGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAAc                                >  1:528952/1‑140 (MQ=255)                                                                                                          ttCCTGCAACCACGGCAGTTGTTCCCAGTGCGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGaa                             >  1:400094/1‑140 (MQ=255)                                                                                                            ccTGCAACCACGGCAGTTGTTCCCAGTGCGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAg                           >  1:250419/1‑140 (MQ=255)                                                                                                                   ccACGGCAGTTGTTCCCAGTGCGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATAcgg                    >  2:533150/1‑140 (MQ=255)                                                                                                                   ccACGGCAGTTGTTCCCAGTGCGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATAcgg                    >  1:782830/1‑140 (MQ=255)                                                                                                                    cACGGCAGTTGTTCCCAGTGCGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATAcggc                   <  2:250419/140‑1 (MQ=255)                                                                                                                           gttgttCCCAGTGCGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCt            >  1:356996/1‑140 (MQ=255)                                                                                                                                 cccAGTGCGGCCGCAGTGGCGTCCTGAGCTGGCGGTTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTGGcgcg      <  1:790040/140‑1 (MQ=255)                                                                                                                                     gtgcGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTGGCGCGTTAc  <  2:400094/140‑1 (MQ=255)                                                                                                                                     gtgcGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTATTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTGGCGCGTTAc  <  2:723763/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         TTCCTGACGGGCGTGATGTCTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTGGCGCGTTAC  >  NC_000913/1112402‑1112673

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑