Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,159,061	C→T	T398I (ACC→ATC)	ptsG →	glucose‑specific PTS enzyme IIBC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,159,061	0	C	T	100.0%	207.5 / NA	59	T398I (ACC→ATC)	ptsG	glucose‑specific PTS enzyme IIBC component
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/31);  total (28/31)

CAAACTGTGGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGC  >  NC_000913/1158924‑1159185                                                                                                                                          |                                                                                                                             caAACTGTGGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTATCa                                                                                                                            >  1:111476/1‑140 (MQ=255)  aaaCTGTGGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTATCAg                                                                                                                           <  2:556239/140‑1 (MQ=255)      tgtgGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTATCAGCGaa                                                                                                                       >  2:860256/1‑140 (MQ=255)      tgtgGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTATCAGCGaa                                                                                                                       >  1:69571/1‑140 (MQ=255)        tgGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTATCAGCGAAAt                                                                                                                     <  2:69571/140‑1 (MQ=255)             gTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTATCAGCGAAATGGCAc                                                                                                                <  2:4962/140‑1 (MQ=255)              ttCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTATCAGCGAAATGGCAcc                                                                                                               >  2:60681/1‑140 (MQ=255)              ttCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACACCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTATCAGCGAAATGGCAcc                                                                                                               >  2:334104/1‑140 (MQ=255)                cccGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTATCAGCGAAATGGCAc                                                                                                                >  2:350527/1‑137 (MQ=255)                cccGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTATCAGCGAAATGGCAc                                                                                                                <  1:350527/137‑1 (MQ=255)                cccGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTATCAGCGAAATGGCACCgg                                                                                                             <  1:574344/140‑1 (MQ=255)                        tcgGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTATCAGCGAAATGGCACCGGCTCTGGtt                                                                                                     >  2:286995/1‑140 (MQ=255)                        tcgGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTATCAGCGAAATGGCACCGGCTCTGGtt                                                                                                     >  1:860494/1‑140 (MQ=255)                                     tGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTATCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTtggtg                                                                                        >  2:624506/1‑140 (MQ=255)                                        gATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTATCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTa                                                                                     <  2:111476/140‑1 (MQ=255)                                          ttGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTATCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTaaa                                                                                   <  1:413640/140‑1 (MQ=255)                                               tactacACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTATCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGaaaa                                                                              <  2:134835/140‑1 (MQ=255)                                                       cATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAACACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACTGGTATCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTa                                                                      >  2:122219/1‑140 (MQ=255)                                                       cATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTATCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTa                                                                      >  1:154442/1‑140 (MQ=255)                                                       cATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTATCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTa                                                                      >  2:648725/1‑140 (MQ=255)                                                       cATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTATCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTa                                                                      >  2:25385/1‑140 (MQ=255)                                                       cATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTATCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTa                                                                      >  2:277721/1‑140 (MQ=255)                                                         tCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTATCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAAc                                                                    >  1:686836/1‑140 (MQ=255)                                                         tCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTATCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAAc                                                                    >  1:227699/1‑140 (MQ=255)                                                         tCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTATCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAAc                                                                    >  1:793838/1‑140 (MQ=255)                                                         tCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTATCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAAc                                                                    >  2:256845/1‑140 (MQ=255)                                                              cgcgTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTATCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAAcc                                                                   <  2:512626/136‑1 (MQ=255)                                                              cgcgTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTATCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAAcc                                                                   >  1:512626/1‑136 (MQ=255)                                                              cgcgTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTATCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGa                                                               >  1:303502/1‑140 (MQ=255)                                                              cgcgTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTATCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGa                                                               >  1:376638/1‑140 (MQ=255)                                                                cgTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACTGGTATCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACg                                                             <  1:122219/140‑1 (MQ=255)                                                                cgTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTATCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACg                                                             <  2:32148/140‑1 (MQ=255)                                                                cgTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTATCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACg                                                             <  2:247709/140‑1 (MQ=255)                                                                cgTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTATCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACg                                                             <  1:286995/140‑1 (MQ=255)                                                                cgTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTATCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACg                                                             <  1:256845/140‑1 (MQ=255)                                                                 gTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTATCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGc                                                            <  2:227699/140‑1 (MQ=255)                                                                  tGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTATCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCa                                                           <  2:376638/140‑1 (MQ=255)                                                                    cTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTATCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATg                                                         <  2:303502/140‑1 (MQ=255)                                                                                 tGGATCTGAAAACACCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTATCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTg                                            <  1:334104/140‑1 (MQ=255)                                                                                   gATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTATCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGc                                                            <  2:865238/122‑1 (MQ=255)                                                                                   gATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTATCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGc                                                            >  1:865238/1‑122 (MQ=255)                                                                                   gATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTATCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCa                                                           <  2:150983/123‑1 (MQ=255)                                                                                   gATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTATCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCa                                                           >  1:150983/1‑123 (MQ=255)                                                                                   gATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTATCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTgcg                                          >  2:12422/1‑140 (MQ=255)                                                                                       tGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTATCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTc                                      <  1:548701/140‑1 (MQ=255)                                                                                        gAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTATCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCa                                     >  1:656825/1‑140 (MQ=255)                                                                                        gAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTATCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCa                                     >  1:377564/1‑140 (MQ=255)                                                                                         aaaaCGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTATCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAg                                    <  2:860494/140‑1 (MQ=255)                                                                                         aaaaCGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTATCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAg                                    >  2:524160/1‑140 (MQ=255)                                                                                             cGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTATCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGt                                               <  2:501452/125‑1 (MQ=255)                                                                                             cGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTATCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGt                                               >  1:501452/1‑125 (MQ=255)                                                                                              gCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTATCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTg                               <  1:648725/140‑1 (MQ=255)                                                                                              gCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTATCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTg                               <  1:25385/140‑1 (MQ=255)                                                                                                   gTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTATCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGAt                          <  2:328383/140‑1 (MQ=255)                                                                                                   gTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTATCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGAt                          <  2:377564/140‑1 (MQ=255)                                                                                                     cGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTATCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGAtgt                        <  2:686836/140‑1 (MQ=255)                                                                                                        gAAGACGCGACTGAAGATGCAAAAGCGACAGGTATCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTc                     <  2:154442/140‑1 (MQ=255)                                                                                                            aCGCGACTGAAGATGCAAAAGCGACAGGTATCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTaaa                 <  1:524160/140‑1 (MQ=255)                                                                                                                           cAAAAGCGACAGGTATCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAggccggc  <  1:12422/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                             CAAACTGTGGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGC  >  NC_000913/1158924‑1159185

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑