Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	69,037	A→G	F338L (TTT→CTT)	araB ←	ribulokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	69,037	0	A	G	100.0%	123.3 / NA	34	F338L (TTT→CTT)	araB	ribulokinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/20);  total (14/20)

CCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGTTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCCGAGTACGCGACCAAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGA  >  NC_000913/68902‑69171                                                                                                                                        |                                                                                                                                       cccATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGTTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCCGAGTACGCGACCAAACCAGGCGTAGATATCACCAAGCGcc                                                                                                                                    >  1:189946/1‑140 (MQ=255)     tGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGTTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCCGAGTACGCGACCAAACCAGGCGTAGATATCACCAAGCGCCGAtt                                                                                                                                <  1:637313/140‑1 (MQ=255)         tCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGTTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCCGAGTACGCGACCAAACCAGGCGTAGATATCACCAAGCGCCGATTGGcc                                                                                                                            <  2:235707/140‑1 (MQ=255)         tCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGTTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCCGAGTACGCGACCAAACCAGGCGTAGATATCACCAAGCGCCGATTGGcc                                                                                                                            <  1:499169/140‑1 (MQ=255)          cGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGTTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCCGAGTACGCGACCAAACCAGGCGTAGATATCACCAAGCGCCGATTGGCCt                                                                                                                           >  2:392822/1‑140 (MQ=255)                 cgcCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGTTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCCGAGTACGCGACCAAACCAGGCGTAGATATCACCAAGCGCCGATTGGCCTGCTTCCa                                                                                                                    >  2:25936/1‑140 (MQ=255)                              tgttTCTGGCTGGCGTTGATTTGCGTTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCCGAGTACGCGACCAAACCAGGCGTAGATATCACCAAGCGCCGATTGGCCTGCTTCCAGACCGATAAATcc                                                                                                       <  1:520887/140‑1 (MQ=255)                                    tggctggcGTTGATTTGCGTTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCCGAGTACGCGACCAAACCAGGCGTAGATATCACCAAGCGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGcac                                                                                                 <  2:438391/140‑1 (MQ=255)                                    tggctggcGTTGATTTGCGTTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCCGAGTACGCGACCAAACCAGGCGTAGATATCACCAAGCGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGcac                                                                                                 <  2:598875/140‑1 (MQ=255)                                         ggcGTTGATTTGCGTTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCCGAGTACGCGACCAAACCAGGCGTAGATATCACCAAGCGCCGATTGGCCTGCTTCCAGACCGATAAATCCAgg                                                                                                    >  2:652986/1‑132 (MQ=255)                                         ggcGTTGATTTGCGTTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCCGAGTACGCGACCAAACCAGGCGTAGATATCACCAAGCGCCGATTGGCCTGCTTCCAGACCGATAAATCCAgg                                                                                                    <  1:652986/132‑1 (MQ=255)                                         ggcGTTGATTTGCGTTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCCGAGTACGCGACCAAACCAGGCGTAGATATCACCAAGCGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGc                                                                                            <  1:25936/140‑1 (MQ=255)                                           cGTTGATTTGCGTTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCCGAGTACGCGACCAAACCAGGCGTAGATATCACCAAGCGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTg                                                                                          >  1:365476/1‑140 (MQ=255)                                           cGTTGATTTGCGTTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCCGAGTACGCGACCAAACCAGGCGTAGATATCACCAAGCGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTg                                                                                          <  1:649625/140‑1 (MQ=255)                                                             gTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCCGAGTACGCGACCAAACCAGGCGTAGATAGCACCAAGCGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCaa                                                                        <  2:515144/140‑1 (MQ=255)                                                               tCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCCGAGTACGCGACCAAACCAGGCGTAGATATCACCAAGCGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAAt                                                                      >  1:451713/1‑140 (MQ=255)                                                                        tGGGCGGCAAGCTGTTCCAGCGGCCAGCCGAGTACGCGACCAAACCAGGCGTAGATATCACCAAGCGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCaa                                                                        >  1:211202/1‑129 (MQ=255)                                                                        tGGGCGGCAAGCTGTTCCAGCGGCCAGCCGAGTACGCGACCAAACCAGGCGTAGATATCACCAAGCGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCaa                                                                        <  2:211202/129‑1 (MQ=255)                                                                        tGGGCGGCAAGCTGTTCCAGCGGCCAGCCGAGTACGCGACCAAACCAGGCGTAGATATCACCAAGCGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAAc                                                             >  1:20063/1‑140 (MQ=255)                                                                            cggcAAGCTGTTCCAGCGGCCAGCCGAGTACGCGACCAAACCAGGCGTAGATATCACCAAGCGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGcc                                                         <  1:392822/140‑1 (MQ=255)                                                                                aaGCTGTTCCAGCGGCCAGCCGAGTACGCGACCAAACCAGGCGTAGATATCACCAAGCGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCt                                                     <  2:459959/140‑1 (MQ=255)                                                                                        ccAGCGGCCAGCCGAGTACGCGACCAAACCAGGCGTAGATATCACCAAGCGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACg                                             <  2:365476/140‑1 (MQ=255)                                                                                                cAGCCGAGTACGCGACCAAACCAGGCGTAGATATCACCAAGCGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGttt                                     >  1:693793/1‑140 (MQ=255)                                                                                                              aCCAAACCAGGCGTAGATATCACCAAGCGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGaa                       <  2:20063/140‑1 (MQ=255)                                                                                                                             gATATCACCAAGCGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAg        <  2:691390/140‑1 (MQ=255)                                                                                                                                  cACCAAGCGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCg                  >  2:602394/1‑125 (MQ=255)                                                                                                                                  cACCAAGCGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCg                  >  2:779445/1‑125 (MQ=255)                                                                                                                                  cACCAAGCGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCg                  <  1:779445/125‑1 (MQ=255)                                                                                                                                  cACCAAGCGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCg                  <  1:602394/125‑1 (MQ=255)                                                                                                                                  cACCAAGCGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGt       >  2:461447/1‑136 (MQ=255)                                                                                                                                  cACCAAGCGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGt       <  1:461447/136‑1 (MQ=255)                                                                                                                                  cACCAAGCGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCg   >  1:603225/1‑140 (MQ=255)                                                                                                                                   aCCAAGCGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGa  >  2:225219/1‑140 (MQ=255)                                                                                                                                   aCCAAGCGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGa  <  2:189946/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                       CCCATGCTTCGGTCAGCGCCGGAAGCAGTTGTTTCTGGCTGGCGTTGATTTGCGTTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCCGAGTACGCGACCAAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGA  >  NC_000913/68902‑69171

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑