Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,166,958	C→T	R292C (CGT→TGT)	ndh →	NADH:quinone oxidoreductase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,166,958	0	C	T	100.0%	112.5 / NA	32	R292C (CGT→TGT)	ndh	NADH:quinone oxidoreductase II
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/14);  total (18/14)

ACCATGGTCACCAGTGCTGATGAAGGCGGCCTGCACACTAAAGATGGCGAATATATTGAGGCTGATCTGATGGTATGGGCAGCCGGGATCAAAGCGCCAGACTTCCTGAAAGATATCGGTGGTCTTGAAACTAACCGTATCAACCAGCTGGTGGTGGAACCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGTTCCGCCGCGTGCTCAGGCTGCACAC  >  NC_000913/1166823‑1167089                                                                                                                                        |                                                                                                                                    aCCATGGTCACCAGTGCTGATGAAGGCGGCCTGCACACTAAAGATGGCGAATATATTGAGGCTGATCTGATGGTATGGGCAGCCGGGATCAAAGCGCCAGACTTCCTGAAAGATATCGGTGGTCTTGAAACTAACTGTAt                                                                                                                                 >  1:668923/1‑140 (MQ=255)      ggTCACCAGTGCTGATGAAGGCGGCCTGCACACTAAAGATGGCGAATATATTGAGGCTGATCTGATGGTATGGGCAGCCGGGATCAAAGCGCCAGACTTCCTGAAAGATATCGGTGGTCTTGAAACTAACTGTATCAAcc                                                                                                                            <  1:814863/140‑1 (MQ=255)                gCTGATGAAGGCGGCCTGCACACTAAAGATGGCGAATATATTGAGGCTGATCTGATGGTATGGGCAGCCGGGATCAAAGCGCCAGACTTCCTGAAAGATATCGGTGGTCTTGAAACTAACTGTATCAACCAGCtggtggt                                                                                                                  >  2:66493/1‑140 (MQ=255)                 cTGATGAAGGCGGCCTGCACACTAAAGATGGCGAATATATTGAGGCTGATCTGATGGTATGGGCAGCCGGGATCAAAGCGCCAGACTTCCTGAAAGATATCGGTGGTCTTGAAACTAACTGTATCAACCAGCtggtggtg                                                                                                                 >  1:599025/1‑140 (MQ=255)                        aGGCGGCCTGCACACTAAAGATGGCGAATATATTGAGGCTGATCTGATGGTATGGGCAGCCGGGATCAAAGCGCCAGACTTCCTGAAAGATATCGGTGGTCTTGAAACTAACTGTATCAACCAGCTGGTGGTGGAACCGa                                                                                                          <  1:325763/140‑1 (MQ=255)                          gcggcCTGCACACTAAAGATGGCGAATATATTGAGGCTGATCTGATGGTATGGGCAGCCGGGATCAAAGCGCCAGACTTCCTGAAAGATATCGGTGGTCTTGAAACTAACTGTATCAACCAGCTGGTGGTGGAACCGACg                                                                                                        <  2:437177/140‑1 (MQ=255)                             gcCTGCACACTAAAGATGGCGAATATATTGAGGCTGATCTGATGGTATGGGCAGCCGGGATCAAAGCGCCAGACTTCCTGAAAGATATCGGTGGTCTTGAAACTAACTGTATCAACCAGCTGGTGGTGGAACCGACGCTg                                                                                                     >  1:542254/1‑140 (MQ=255)                              ccTGCACACTAAAGATGGCGAATATATTGAGGCTGATCTGATGGTATGGGCAGCCGGGATCAAAGCGCCAGACTTCCTGAAAGATATCGGTGGTCTTGAAACTAACTGTATCAACCAGGTGGTGGTGGAACCGACGCTGc                                                                                                    >  2:788030/1‑140 (MQ=255)                                       tAAAGATGGCGAATATATTGAGGCTGATCTGATGGTATGGGCAGCCGGGATCAAAGCGCCAGACTTCCTGAAAGATATCGGTGGTCTTGAAACTAACTGTATCAACCAGCTGGTGGTGGAACCGACGCTGCAAACCAccc                                                                                           >  2:35607/1‑140 (MQ=255)                                            aTGGCGAATATATTGAGGCTGATCTGATGGTATGGGCAGCCGGGATCAAAGCGCCAGACTTCCTGAAAGATATCGGTGGTCTTGAAACTAACTGTATCAACCAGCTGGTGGTGGAACCGACGCTGCAAACCACCCGCGAt                                                                                      >  1:504805/1‑140 (MQ=255)                                               gCGAATATATTGAGGCTGATCTGATGGTATGGGCAGCCGGGATCAAAGCGCCAGACTTCCTGAAAGATATCGGTGGTCTTGAAACTAACTGTATCAACCAGCTGGTGGTGGAACCGACGCTGCAAACCACCCGCGATCCa                                                                                   >  1:179818/1‑140 (MQ=255)                                                       atTGAGGCTGATCTGATGGTATGGGCAGCCGGGATCAAAGCGCCAGACTTCCTGAAAGATATCGGTGGTCTTGAAACTAACTGTATCAACCAGCTGGTGGTGGAACCGACGCTGCAAACCACCCGCGATCCAGACATTTa                                                                           <  2:599025/140‑1 (MQ=255)                                                         tGAGGCTGATCTGATGGTATGGGCAGCCGGGATCAAAGCGCCAGACTTCCTGAAAGATATCGGTGGTCTTGAAACTAACTGTATCAACCAGCTGGTGGTGGAACCGACGCTGCAAACCACCCGCGATCCAGACATTTACg                                                                         >  2:676118/1‑140 (MQ=255)                                                                 atctgatGGTATGGGCAGCCGGGATCAAAGCGCCAGACTTCCTGAAAGATATCGGTGGTCTTGAAACTAACTGTATCAACCAGCTGGTGGTGGAACCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGc                                                                 <  2:542254/140‑1 (MQ=255)                                                                      atGGTATGGGCAGCCGGGATCAAAGCGCCAGACTTCCTGAAAGATATCGGTGGTCTTGAAACTAACTGTATCAACCAGCTGGTGGTGGAACCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTg                                                            >  1:518076/1‑140 (MQ=255)                                                                           aTGGGCAGCCGGGATCAAAGCGCCAGACTTCCTGAAAGATATCGGTGGTCTTGAAACTAACTGTATCAACCAGCTGGTGGTGGAACCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTg                                                            >  2:840426/1‑135 (MQ=255)                                                                           aTGGGCAGCCGGGATCAAAGCGCCAGACTTCCTGAAAGATATCGGTGGTCTTGAAACTAACTGTATCAACCAGCTGGTGGTGGAACCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTg                                                            <  1:840426/135‑1 (MQ=255)                                                                                cAGCCGGGATCAAAGCGCCAGACTTCCTGAAAGATATCGGTGGTCTTGAAACTAACTGTATCAACCAGCTGGTGGTGGAACCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTg                                                            <  2:572579/130‑1 (MQ=255)                                                                                cAGCCGGGATCAAAGCGCCAGACTTCCTGAAAGATATCGGTGGTCTTGAAACTAACTGTATCAACCAGCTGGTGGTGGAACCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTg                                                            >  1:572579/1‑130 (MQ=255)                                                                                   ccGGGATCAAAGCGCCAGACTTCCTGAAAGATATCGGTGGTCTTGAAACTAACTGTATCAACCAGCTGGTGGTGGAACCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCcg                                               >  1:87769/1‑140 (MQ=255)                                                                                      ggATCAAAGCGCCAGACTTCCTGAAAGATATCGGTGGTCTTGAAACTAACTGTATCAACCAGCTGGTGGTGGAACCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGt                                            >  2:468180/1‑140 (MQ=255)                                                                                              gcgcCAGACTTCCTGAAAGATATCGGTGGTCTTGAAACTAACTGTATCAACCAGCTGGTGGTGGAACCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAgg                                    <  1:35607/140‑1 (MQ=255)                                                                                                              aaGATATCGGTGGTCTTGAAACTAACTGTATCAACCAGCTGGTGGTGGAACCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGTTccgccg                    <  1:468180/140‑1 (MQ=255)                                                                                                                    tCGGTGGTCTTGAAACTAACTGTATCAACCAGCTGGTGGTGGAACCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGTTCCGCCGCGTGCt              <  1:589470/140‑1 (MQ=255)                                                                                                                     cGGTGGTCTTGAAACTAACTGTATCAACCAGCTGGTGGTGGAACCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGTTCCGCCGCGTGCTc             <  2:594532/140‑1 (MQ=255)                                                                                                                       gtggtCTTGAAACTAACTGTATCAACCAGCTGGTGGTGGAACCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGTTccgcc                     <  1:654846/130‑1 (MQ=255)                                                                                                                       gtggtCTTGAAACTAACTGTATCAACCAGCTGGTGGTGGAACCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGTTccgcc                     >  2:654846/1‑130 (MQ=255)                                                                                                                           tCTTGAACCTAACTGTATCAACCAGCTGGTGGTGGAACCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGTTCCGCCGCGTGCTCAgg          <  1:592075/137‑1 (MQ=255)                                                                                                                           tCTTGAAACTAACTGTATCAACCAGCTGGTGGTGGAACCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGTTCCGCCGCGTGCTCAgg          >  2:592075/1‑137 (MQ=255)                                                                                                                            cTTGAAACTAACTGTATCAACCAGCTGGTGGTGGAACCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGTTCCGCCGCGTGCTCAGGCTGc      >  1:117960/1‑140 (MQ=255)                                                                                                                             ttGAAACTAACTGTATCAACCAGCTGGTGGTGGAACCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGTTCCGCCGCGTGCTCAGGCTGca     >  1:839971/1‑140 (MQ=255)                                                                                                                                aaaCTAACTGTATCAACCAGCTGGTGGTGGAACCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGTTCCGCCGCGTGCTCAGGCTGCacac  <  2:504805/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                    ACCATGGTCACCAGTGCTGATGAAGGCGGCCTGCACACTAAAGATGGCGAATATATTGAGGCTGATCTGATGGTATGGGCAGCCGGGATCAAAGCGCCAGACTTCCTGAAAGATATCGGTGGTCTTGAAACTAACCGTATCAACCAGCTGGTGGTGGAACCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGTTCCGCCGCGTGCTCAGGCTGCACAC  >  NC_000913/1166823‑1167089

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑