Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,388,183	A→G	K488K (AAA→AAG)	tyrR →	DNA‑binding transcriptional dual regulator TyrR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,388,183	0	A	G	100.0%	125.0 / NA	35	K488K (AAA→AAG)	tyrR	DNA‑binding transcriptional dual regulator TyrR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/20);  total (15/20)

GATTATGACGCCGCAACGGTAGCCGTGGGCGAAGATGCGATGGAAGGTTCGCTGGACGAAATCACCAGCCGTTTTGAACGCTCGGTATTAACCCAGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGG  >  NC_000913/1388049‑1388295                                                                                                                                       |                                                                                                                 gATTATGACGCCGCAACGGTAGCCGTGGGCGAAGATGCGATGGAAGGTTCGCTGGACGAAATCACCAGCCGTTTTGAACGCTCGGTATTAACCCAGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAGCGtct                                                                                                             <  1:81826/140‑1 (MQ=255)   ttATGACGCCGCAACGGTAGCCGTGGGCGAAGATGCGATGGAAGGTTCGCTGGACGAAATCACCAGCCGTTTTGAACGCTCGGTATTAACCCAGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAGCGTCTCg                                                                                                           <  2:121312/140‑1 (MQ=255)   ttATGACGCCGCAACGGTAGCCGTGGGCGAAGATGCGATGGAAGGTTCGCTGGACGAAATCACCAGCCGTTTTGAACGCTCGGTATTAACCCAGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAGCGTCTCg                                                                                                           <  2:452893/140‑1 (MQ=255)     aTGACGCCGCAACGGTAGCCGTGGGCGAAGATGCGATGGAAGGTTCGCTGGACGAAATCACCAGCCGTTTTGAACGCTCGGTATTAACCCAGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAGCGTCTCGGc                                                                                                         <  1:384964/140‑1 (MQ=255)         cgccgcAACGGTAGCCGTGGGCGAAGATGCGATGGAAGGTTCGCTGGACGAAATCACCAGCCGTTTTGAACGCTCGGTATTAACCCAGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAGCGTCTCGGCGttt                                                                                                     <  2:555407/140‑1 (MQ=255)            cgcAACGGTAGCCGTGGGCGAAGATGCGATGGAAGGTTCGCTGGACGAAATCACCAGCCGTTTTGAACGCTCGGTATTAACCCAGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAGCGTCTCGGCGTTTcac                                                                                                  >  2:615364/1‑140 (MQ=255)               aaCGGTAGCCGTGGGCGAAGATGCGATGGAAGGTTCGCTGGACGAAATCACCAGCCGTTTTGAACGCTCGGTATTAACCCAGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAGCGTCTCGGCGTTTCACATa                                                                                               >  2:307359/1‑140 (MQ=255)                          tGGGCGAAGATGCGATGGAAGGTTCGCTGGACGAAATCACCAGCCGTTTTGAACGCTCGGTATTAACCCAGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAGCGTCTCGGCGTTTCACATACCGCGATTGcc                                                                                    >  1:161112/1‑140 (MQ=255)                            ggCGAAGATGCGATGGAAGGTTCGCTGGACGAAATCACCAGCCGTTTTGAACGCTCGGTATTAACCCAGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAGCGTCTCGGCGTTTCACATACCGCGATTGCCaa                                                                                  >  2:329830/1‑140 (MQ=255)                             gCGAAGATGCGATGGAAGGTTCGCTGGACGAAATCACCAGCCGTTTTGAACGCTCGGTATTAACCCAGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAGCGTCTCGGCGTTTCACATACCGCGATTGCCAAt                                                                                 <  2:802962/140‑1 (MQ=255)                                     gCGATGGAAGGTTCGCTGGACGAAATCACCAGCCGTTTTGAACGCTCGGTATTAACCCAGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAGCGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCg                                                                         <  1:311811/140‑1 (MQ=255)                                           gAAGGTTCGCTGGACGAAATCACCAGCCGTTTTGAACGCTCGGTATTAACCCAGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAGCGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAata                                                                   >  2:284490/1‑140 (MQ=255)                                              ggTTCGCTGGACGAAATCACCAGCCGTTTTGAACGCTCGGTATTAACCCAGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAGCGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATgg                                                                <  1:615364/140‑1 (MQ=255)                                              ggTTCGCTGGACGAAATCACCAGCCGTTTTGAACGCTCGGTATTAACCCAGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAGCGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATgg                                                                <  2:296387/140‑1 (MQ=255)                                                     tGGACGAAATCACCAGCCGTTTTGAACGCTCGGTATTAACCCAGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAGCGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGt                                                         <  2:419559/140‑1 (MQ=255)                                                       gACGAAATCACCAGCCGTTTTGAACGCTCGGTATTAACCCAGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAGCGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCa                                                       >  1:679207/1‑140 (MQ=255)                                                        aCGAAATCACCAGCCGTTTTGAACGCTCGGTATTAACCCAGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAGCGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCag                                                      <  1:329830/140‑1 (MQ=255)                                                         cGAAATCACCAGCCGTTTTGAACGCTCGGTATTAACCCAGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAGCGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAata                                                                   >  1:379893/1‑126 (MQ=255)                                                         cGAAATCACCAGCCGTTTTGAACGCTCGGTATTAACCCAGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAGCGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAata                                                                   <  2:379893/126‑1 (MQ=255)                                                                 ccAGCCGTTTTGAACGCTCGGTATTAACCCAGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAGCGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCt                                                             >  1:720522/1‑124 (MQ=255)                                                                 ccAGCCGTTTTGAACGCTCGGTATTAACCCAGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAGCGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCt                                                             <  2:720522/124‑1 (MQ=255)                                                                          ttGAACGCTCGGTATTAACCCAGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAGCGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTaa                                    >  2:286141/1‑140 (MQ=255)                                                                            gAACGCTCGGTATTAACCCAGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAGCGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAgc                                  <  1:286141/140‑1 (MQ=255)                                                                                          aaCCCAGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAGCGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGa                    <  1:284490/140‑1 (MQ=255)                                                                                            cccAGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAGCGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATg                  >  1:206551/1‑140 (MQ=255)                                                                                               aGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAGCGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTg               <  2:161112/140‑1 (MQ=255)                                                                                                gCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAGCGTCTCGGCGTTTCGCATACCGCGATTGCCTATAAGTTGCGTGAATATGGTCTAAGTCAGAAGAAGATCGAAGAGTAAGGGCGAATATGCCTGATGGTGc              >  2:511787/1‑140 (MQ=255)                                                                                                gCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAGCGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGt                >  2:491789/1‑138 (MQ=255)                                                                                                gCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAGCGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGt                <  1:491789/138‑1 (MQ=255)                                                                                                gCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAGCGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGc              >  2:317555/1‑140 (MQ=255)                                                                                                gCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAGCGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGc              >  2:410200/1‑140 (MQ=255)                                                                                                gCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAGCGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGc              >  2:533792/1‑140 (MQ=255)                                                                                                gCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAGCGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGc              >  2:582350/1‑140 (MQ=255)                                                                                                      tCGCAATTATCCCAGCACGCGCAAACTGGCAAAGCGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACAcc        <  2:308196/140‑1 (MQ=255)                                                                                                           aTTATCCCAGCACGCGCAAACTGGCAAAGCGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAg   <  1:511787/140‑1 (MQ=255)                                                                                                           aTTATCCCAGCACGCGCAAACTGGCAAAGCGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAg   <  2:679207/140‑1 (MQ=255)                                                                                                            ttATCCCAGCACGCGCAAACTGGCAAAGCGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAgg  <  2:206551/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                 GATTATGACGCCGCAACGGTAGCCGTGGGCGAAGATGCGATGGAAGGTTCGCTGGACGAAATCACCAGCCGTTTTGAACGCTCGGTATTAACCCAGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGG  >  NC_000913/1388049‑1388295

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑