Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,401,564	A→G	S70S (AGT→AGC)	abgT ←	p‑aminobenzoyl glutamate:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,401,564	0	A	G	100.0%	171.7 / NA	47	S70S (AGT→AGC)	abgT	p‑aminobenzoyl glutamate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/24);  total (23/24)

CATTAGTGCTGGCAGTAAGCCGACGCGCTCCGCCAGACCGGCACCTAAAACCAGCGCCAGGATCGCACCAAGTGGAGCAAAACCGCTAAAGTTTTTAATAACATTGGGTAAAAACCAGTGTAATCCTTCCACACTGAGCAGGTTTTTCACCACGACCGGCGTACCATCGGTCGGGTTTTTCGCACTGACGCCAAAGGCCGACAAAATTGCCGTCGTCACCATGAGTACGATAATCAAATAGATAAAGAGCAGAAAAGGATGGGGA  >  NC_000913/1401432‑1401696                                                                                                                                     |                                                                                                                                     cATTAGTGCTGGCAGTAAGCCGACGCGCTCCGCCAGACCGGCACCTAAAACCAGCGCCAGGATCGCACCAAGTGGAGCAAAACCGCTAAAGTTTTTAATAACATTGGGTAAAAACCAGTGTAATCCTTCCACGCTGAGCa                                                                                                                               <  1:671482/140‑1 (MQ=255)   ttAGTGCTGGCAGTAAGCCGACGCGCTCCGCCAGACCGGCACCTAAAACCAGCGCCAGGATCGCACCAAGTGGAGCAAAACCGCTAAAGTTTTTAATAACATTGGGTAAAAACCAGTGTAATCCTTCCACGCTGAGCAgg                                                                                                                             >  2:228698/1‑140 (MQ=255)           ggCAGTAAGCCGACGCGCTCCGCCAGACCGGCACCTAAAACCAGCGCCAGGATCGCACCAAGTGGAGCAAAACCGCTAAAGTTTTTAATAACATTGGGTAAAAACCAGTGTAATCCTTCCACGCTGAGCAGGTTTTTcac                                                                                                                     >  2:649641/1‑140 (MQ=255)           ggCAGTAAGCCGACGCGCTCCGCCAGACCGGCACCTAAAACCAGCGCCAGGATCGCACCAAGTGGAGCAAAACCGCTAAAGTTTTTAATAACATTGGGTAAAAA‑CAGTGTAATCCTTCCACGCTGa                                                                                                                                  >  2:548418/1‑126 (MQ=255)           ggCAGTAAGCCGACGCGCTCCGCCAGACCGGCACCTAAAACCAGCGCCAGGATCGCACCAAGTGGAGCAAAACCGCTAAAGTTTTTAATAACATTGGGTAAAAA‑CAGTGTAATCCTTCCACGCTGa                                                                                                                                  <  1:548418/126‑1 (MQ=255)             cAGTAAGCCGACGCGCTCCGCCAGACCGGCACCTAAAACCAGCGCCAGGATCGCACCAAGTGGAGCAAAACCGCTAAAGTTTTTAATAACATTGGGTAAAAACCAGTGTAATCCTTCCACGCTGAGCAGGTTTTTcacca                                                                                                                   >  1:58294/1‑140 (MQ=255)               gTAAGCCGACGCGCTCCGCCAGACCGGCACCTAAAACCAGCGCCAGGATCGCACCAAGTGGAGCAAAACCGCTAAAGTTTTTAATAACATTGGGTAAAAACCAGTGTAATCCTTCCACGCTGAGCAGGTTTTTCACCACg                                                                                                                 <  2:69627/140‑1 (MQ=255)                 aaGCCGACGCGCTCCGCCAGACCGGCACCTAAAACCAGCGCCAGGATCGCACCAAGTGGAGCAAAACCGCTAAAGTTTTTAATAACATTGGGTAAAAACCAGTGTAATCCTTCCACGCTGAGCAGGTTTTTCACCACGAc                                                                                                               <  2:248244/140‑1 (MQ=255)                  aGCCGACGCGCTCCGCCAGACCGGCACCTAAAACCAGCGCCAGGATCGCACCAAGTGGAGCAAAACCGCTAAAGTTTTTAATAACATTGGGTAAAAACCAGTGTAATCCTTCCACGCTGAGCAGGTTTTTcac                                                                                                                     >  2:380657/1‑133 (MQ=255)                  aGCCGACGCGCTCCGCCAGACCGGCACCTAAAACCAGCGCCAGGATCGCACCAAGTGGAGCAAAACCGCTAAAGTTTTTAATAACATTGGGTAAAAACCAGTGTAATCCTTCCACGCTGAGCAGGTTTTTcac                                                                                                                     <  1:380657/133‑1 (MQ=255)                   gCCGACGCGCTCCGCCAGACCGGCACCTAAAACCAGCGCCAGGATCGCACCAAGTGGAGCAAAACCGCTAAAGTTTTTAATAACATTGGGTAAAAACCAGTGTAATCCTTCCACGCTGAGCAGGTTTTTCACCACg                                                                                                                 >  2:185204/1‑136 (MQ=255)                   gCCGACGCGCTCCGCCAGACCGGCACCTAAAACCAGCGCCAGGATCGCACCAAGTGGAGCAAAACCGCTAAAGTTTTTAATAACATTGGGTAAAAACCAGTGTAATCCTTCCACGCTGAGCAGGTTTTTCACCACg                                                                                                                 <  1:185204/136‑1 (MQ=255)                        cgcgCTCCGCCAGACCGGCACCTAAAACCAGCGCCAGGATCGCACCAAGTGGAGCAAAACCGCTAAAGTTTTTAATAACATTGGGTAAAAACCAGTGTAATCCTTCCACGCTGAGCAGGTTTTTCACCACGACCGGCGTa                                                                                                        >  1:104724/1‑140 (MQ=255)                                     aCCGGCACCTAAAACCAGCGCCAGGATCGCACCAAGTGGAGCAAAACCGCTAAAGTTTTTAATAACATTGGGTAAAAACCAGTGTAATCCTTCCACGCTGAGCAGGTTTTTCACCACGACCGGCGTACCATCGGTCGGGt                                                                                           >  2:610200/1‑140 (MQ=255)                                         gCACCTAAAACCAGCGCCAGGATCGCACCAAGTGGAGCAAAACCGCTAAAGTTTTTAATAACATTGGGTAAAAACCAGTGTAATCCTTCCACGCTGAGCAGGTTTTTCACCACGACCGGCGTACCATCGGTCGGGttttt                                                                                       >  1:37278/1‑140 (MQ=255)                                                       cgcCAGGATCGCACCAAGTGGAGCAAAACCGCTAAAGTTTTTAATAACATTGGGTAAAAACCAGTGTAATCCTTCCACGCTGAGCAGGTTTTTCACCACGACCGGCGTACCATCGGTCGGGTTTTTCGCACTGACGCCaa                                                                         >  1:190535/1‑140 (MQ=255)                                                              aTCGCACCAAGTGGAGCAAAACCGCTAAAGTTTTTAATAACATTGGGTAAAAACCAGTGTAATCCTTCCACGCTGAGCAGGTTTTTCACCACGACCGGCGTACCATCGGTCGGGTTTTTCGCACTGACGCCAAAGGCCGa                                                                  <  1:257462/140‑1 (MQ=255)                                                                     cAAGTGGAGCAAAACCGCTAAAGTTTTTAATAACATTGGGTAAAAACCAGTGTAATCCTTCCACGCTGAGCAGGTTTTTCACCACGACCGGCGTACCATCGGTCGGGTTTTTCGCACTGACGCCAAAGGCCGACAAAAtt                                                           <  2:190535/140‑1 (MQ=255)                                                                        gTGGAGCAAAACCGCTAAAGTTTTTAATAACATTGGGTAAAAACCAGTGTAATCCTTCCACGCTGAGCAGGTTTTTCACCACGACCGGCGTACCATCGGTCGGGTTTTTCGCACTGACGCCAAAGGCCGACAAAATTGcc                                                        >  1:796759/1‑140 (MQ=255)                                                                         tGGAGCAAAACCGCTAAAGTTTTTAATAACATTGGGTAAAAACCAGTGTAATCCTTCCACGCTGAGCAGGTTTTTCACCACGACCGGCGTACCATCGGTCGGGTTTTTCGCACTGACGCCAAAGGCCGACAAAATTGCcg                                                       <  2:796759/140‑1 (MQ=255)                                                                         tGGAGCAAAACCGCTAAAGTTTTTAATAACATTGGGTAAAAACCAGTGTAATCCTTCCACGCTGAGCAGGTTTTTCACCACGACCGGCGTACCATCGGTCGGGTTTTTCGCACTGACGCCAAAGGCCGACAAAATTGCcg                                                       <  2:794817/140‑1 (MQ=255)                                                                             gCAAAACCGCTAAAGTTTTTAATAACATTGGGTAAAAACCAGTGTAATCCTTCCACGCTGAGCAGGTTTTTCACCACGACCGGCGTACCATCGGTCGGGTTTTTCGCACTGACGCCAAAGGCCGACAAAATTGCcgtcgt                                                   <  2:498328/140‑1 (MQ=255)                                                                             gCAAAACCGCTAAAGTTTTTAATAACATTGGGTAAAAACCAGTGTAATCCTTCCACGCTGAGCAGGTTTTTCACCACGACCGGCGTACCATCGGTCGGGTTTTTCGCACTGACGCCAAAGGCCGACAAAATTGCcgtcgt                                                   >  2:781155/1‑140 (MQ=255)                                                                                aaaCCGCTAAAGTTTTTAATAACATTGGGTAAAAACCAGTGTAATCCTTCCACGCTGAGCAGGTTTTTCACCACGACCGGCGTACCATCGGTCGGGTTTTTCGCACTGACGCCAAAGGCCGACAAAATTGCCGTCGTCAc                                                <  2:37278/140‑1 (MQ=255)                                                                                 aaCCGCTAAAGTTTTTAATAACATTGGGTAAAAACCAGTGTAATCCTTCCACGCTGAGCAGGTTTTTCACCACGACCGGCGTACCATCGGTCGGGTTTTTCGCACTGACGCCAAAGGCCGACAAAATTGCCGTCGTCAcc                                               <  2:794745/140‑1 (MQ=255)                                                                                 aaCCGCTAAAGTTTTTAATAACATTGGGTAAAAACCAGTGTAATCCTTCCACGCTGAGCAGGTTTTTCACCACGACCGGCGTACCATCGGTCGGGTTTTTCGCACTGACGCCAAAGGCCGACAAAATTGCCGTCGTCAcc                                               >  2:222396/1‑140 (MQ=255)                                                                                 aaCCGCTAAAGTTTTTAATAACATTGGGTAAAAACCAGTGTAATCCTTCCACGCTGAGCAGGTTTTTCACCACGACCGGCGTACCATCGGTCGGGTTTTTCGCACTGACGCCAAAGGCCGACAAAATTGCCGTCGTCAcc                                               <  2:104724/140‑1 (MQ=255)                                                                                   ccGCTAAAGTTTTTAATAACATTGGGTAAAAACCAGTGTAATCCTTCCACGCTGAGCAGGTTTTTCACCACGACCGGCGTACCATCGGTCGGGTTTTTCGCACTGACGCCAAAGGCCGACAAAATTGCCGTCGTCACCAt                                             >  2:748050/1‑140 (MQ=255)                                                                                   ccGCTAAAGTTTTTAATAACATTGGGTAAAAACCAGTGTAATCCTTCCACGCTGAGCAGGTTTTTCACCACGACCGGCGTACCATCGGTCGGGTTTTTCGCACTGACGCCAAAGGCCGACAAAATTGCCGTCGTCACCAt                                             >  1:227502/1‑140 (MQ=255)                                                                                             ttttAATAACATTGGGTAAAAACCAGTGTAATCCTTCCACGCTGAGCAGGTTTTTCACCACGACCGGCGTACCATCGGTCGGGTTTTTCGCACTGACGCCAAAGGCCGACAAAATTGCCGTCGTCACCATGAGTACGATa                                   <  1:649641/140‑1 (MQ=255)                                                                                                      cATTGGGTAAAAACCAGTGTAATCCTTCCACGCTGAGCAGGTTTTTCACCACGACCGGCGTACCATCGGTCGGGTTTTTCGCACTGACGCCAAAGGCCGACAAAATTGCCGTCGTCACCATGAGTACGATAATCAAATAg                          >  2:474133/1‑140 (MQ=255)                                                                                                         tGGGTAAAAACCAGTGTAATCCTTCCACGCTGAGCAGGTTTTTCACCACGACCGGCGTACCATCGGTCGGGTTTTTCGCACTGACGCCAAAGGTCGACAAAATTGCCGTCGTCACCATGAGTACGATAATCAAATAGATa                       <  1:610200/140‑1 (MQ=255)                                                                                                           ggTAAAAACCAGTGTAATCCTTCCACGCTGAGCAGGTTTTTCACCACGACCGGCGTACCATCGGTCGGGTTTTTCGCACTGACGCCAAAGGCCGACAAAATTGCCGTCGTCACCATGAGTACGATAATCAAATAGATaaa                     >  1:72747/1‑140 (MQ=255)                                                                                                           ggTAAAAACCAGTGTAATCCTTCCACGCTGAGCAGGTTTTTCACCACGACCGGCGTACCATCGGTCGGGTTTTTCGCACTGACGCCAAAGGCCGACAAAATTGCCGTCGTCACCATGAGTACGATAATCAAATAGATaaa                     >  1:333897/1‑140 (MQ=255)                                                                                                                 aaCCAGTGTAATCCTTCCACGCTGAGCAGGTTTTTCACCACGACCGGCGTACCATCGGTCGGGTTTTTCGCACTGACGCCAAAGGCCGACAAAATTGCCGTCGTCACCATGAGTACGATAATCAAATAGATAAAGAGCAg               >  2:855880/1‑140 (MQ=255)                                                                                                                   ccAGTGTAATCCTTCCACGCTGAGCAGGTTTTTCACCACGACCGGCGTACCATCGGTCGGGTTTTTCGCACTGACGCCAAAGGCCGACAAAATTGCCGTCGTCACCATGAGTACGATAATCAAATAGATAAAGAGCAGaa             <  2:469226/140‑1 (MQ=255)                                                                                                                     aGTGTAATCCTTCCACGCTGAGCAGGTTTTTCACCACGACCGGCGTACCATCGGTCGGGTTTTTCGCACTGACGCCAAAGGCCGACAAAATTGCCGTCGTCACCATGAGTACGATAATCAAATAGATAAAGAGCAGaaaa           <  1:222396/140‑1 (MQ=255)                                                                                                                      gtgtAATCCTTCCACGCTGAGCAGGTTTTTCACCACGACCGGCGTACCATCGGTCGGGTTTTTCGCACTGACGCCAAAGGCCGACAAAATTGCCGTCGTCACCATGAGTACGATAATCAAATAGATAAAGAGCAGAAAAg          <  2:666759/140‑1 (MQ=255)                                                                                                                      gtgtAATCCTTCCACGCTGAGCAGGTTTTTCACCACGACCGGCGTACCATCGGTCGGGTTTTTCGCACTGACGCCAAAGGCCGACAAAATTGCCGTCGTCACCATGAGTACGATAATCAAATAGATAAAGAGCAGAAAAg          <  2:227502/140‑1 (MQ=255)                                                                                                                       tgtAATCCTTCCACGCTGAGCAGGTTTTTCACCACGACCGGCGTACCATCGGTCGGGTTTTTCGCACTGACGCCAAAGGCCGACAAAATTGCCGTCGTCACCATGAGTACGATAATCAAATAGATAAAGAGCAGAAAAgg         <  2:47732/140‑1 (MQ=255)                                                                                                                       tgtAATCCTTCCACGCTGAGCAGGTTTTTCACCACGACCGGCGTACCATCGGTCGGGTTTTTCGCACTGACGCCAAAGGCCGACAAAATTGCCGTCGTCACCATGAGTACGATAATCAAATAGATAAAGAGCAGAAAAgg         >  1:73895/1‑140 (MQ=255)                                                                                                                         tAATCCTTCCACGCTGAGCAGGTTTTTCACCACGACCGGCGTACCATCGGTCGGGTTTTTCGCACTGACGCCAAAGGCCGACAAAATTGCCGTCGTCACCATGAGTACGATAATCAAATAGATAAAGAGCAGaaaa           >  2:634866/1‑136 (MQ=255)                                                                                                                         tAATCCTTCCACGCTGAGCAGGTTTTTCACCACGACCGGCGTACCATCGGTCGGGTTTTTCGCACTGACGCCAAAGGCCGACAAAATTGCCGTCGTCACCATGAGTACGATAATCAAATAGATAAAGAGCAGaaaa           <  1:634866/136‑1 (MQ=255)                                                                                                                              cTTCCACGCTGAGCAGGTTTTTCACCACGACCGGCGTACCATCGGTCGGGTTTTTCGCACTGACGCCAAAGGCCGACAAAATTGCCGTCGTCACCATGAGTACGATAATCAAATAGATAAAGAGCAGAAAAGGATGGGGa  <  1:748050/140‑1 (MQ=255)                                                                                                                                 ccACGCTGAGCAGGTTTTTCACCACGACCGGCGTACCATCGGTCGGGTTTTTCGCACTGACGCCAAAGGCCGACAAAATTGCCGTCGTCACCATGAGTACGATAATCAAATAGATAAAGAGCAGaa             <  1:276253/126‑1 (MQ=255)                                                                                                                                 ccACGCTGAGCAGGTTTTTCACCACGACCGGCGTACCATCGGTCGGGTTTTTCGCACTGACGCCAAAGGCCGACAAAATTGCCGTCGTCACCATGAGTACGATAATCAAATAGATAAAGAGCAGaa             >  2:276253/1‑126 (MQ=255)                                                                                                                                 ccACGCTGAGCAGGTTTTTCACCACGACCGGCGTACCATCGGTCGGGTTTTTCGCACTGACGCCAAAGGCCGACAAAATTGCCGTCGTCACCATGAGTACGATAATCAAATAGATAAAGAGCAGaa             <  1:103622/126‑1 (MQ=255)                                                                                                                                 ccACGCTGAGCAGGTTTTTCACCACGACCGGCGTACCATCGGTCGGGTTTTTCGCACTGACGCCAAAGGCCGACAAAATTGCCGTCGTCACCATGAGTACGATAATCAAATAGATAAAGAGCAGaa             >  2:103622/1‑126 (MQ=255)                                                                                                                                     |                                                                                                                                     CATTAGTGCTGGCAGTAAGCCGACGCGCTCCGCCAGACCGGCACCTAAAACCAGCGCCAGGATCGCACCAAGTGGAGCAAAACCGCTAAAGTTTTTAATAACATTGGGTAAAAACCAGTGTAATCCTTCCACACTGAGCAGGTTTTTCACCACGACCGGCGTACCATCGGTCGGGTTTTTCGCACTGACGCCAAAGGCCGACAAAATTGCCGTCGTCACCATGAGTACGATAATCAAATAGATAAAGAGCAGAAAAGGATGGGGA  >  NC_000913/1401432‑1401696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑