Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,422,369	T→C	G129G (GGT→GGC)	ydaV →	Rac prophage; putative ATP‑binding protein YdaV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,422,369	0	T	C	100.0%	128.0 / NA	36	G129G (GGT→GGC)	ydaV	Rac prophage; putative ATP‑binding protein YdaV
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/20);  total (16/20)

ATACGCGTTGAGTCAGGCGAAATCCATCGCTGATGAACTGATGACCGGGTGTACAAATTTTGCGTTCAGCGGAAAACCTGGTACCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGTCAGACAGTGATTGTGGTTACCGTGGCTGATGTTATGAGTGCCCTGCACGCCAGCTATGACGATGGGCAGTCAGGCGAAAAATTTTTGCGGGAACTGTGCGAAGTGGATCTGCTGGTTCTTGATGAAA  >  NC_000913/1422234‑1422496                                                                                                                                        |                                                                                                                                aTACGCGTTGAGTCAGGCGAAATCCATCGCTGATGAACTGATGACCGGGTGTACAAATTTTGCGTTCAGCGGAAAACCTGGTACCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGCCAGa                                                                                                                             <  1:257610/140‑1 (MQ=255)  tACGCGTTGAGTCAGGCGAAATCCATCGCTGATGAACTGATGACCGGGTGTACAAATTTTGCGTTCCGCGGAAACCCTGGTACCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGCCAGAc                                                                                                                            <  2:750580/140‑1 (MQ=255)  tACGCGTTGAGTCAGGCGAAATCCATCGCTGATGAACTGATGACCGGGTGTACAAATTTTGCGTTCAGCGGAAAACCTGGTACCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGCCAGAc                                                                                                                            <  2:151469/140‑1 (MQ=255)         tGAGTCAGGCGAAATCCATCGCTGATGAACTGATGACCGGGTGTACAAATTTTGCGTTCAGCGGAAAACCTGGGACCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGCCAGACAGTGAtt                                                                                                                     >  2:639779/1‑140 (MQ=255)           aGTCAGGCGAAATCCATCGCTGATGAACTGATGACCGGGTGTACAAATTTTGCGTTCAGCGGAAAACCTGGTACCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGCCAGACAGTGATtgt                                                                                                                   >  2:73709/1‑140 (MQ=255)             tCAGGCGAAATCCATCGCTGATGAACTGATGACCGGGTGTACAAATTTTGCGTTCAGCGGAAAACCTGGGACCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGCCAGACAGTGATTGTgg                                                                                                                 <  1:639779/140‑1 (MQ=255)               aGGCGAAATCCATCGCTGATGAACTGATGACCGGGTGTACAAATTTTGCGTTCAGCGGAGAACCTGGTACCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGCCAGACAGTGATTGTGGtt                                                                                                               >  1:74751/1‑140 (MQ=255)                ggCGAAATCCATCGCTGATGAACTGATGACCGGGTGTACAAATTTTGCGTTCAGCGGAAAACCTGGTACCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGCCAGACAGTGATTGTGGTTa                                                                                                              >  2:78666/1‑140 (MQ=255)                ggCGAAATCCATCGCTGATGAACTGATGACCGGGTGTACAAATTTTGCGTTCAGCGGAAAACCTGGTACCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGCCAGACAGTGATTGTGGTTa                                                                                                              >  1:422893/1‑140 (MQ=255)                      aTCCATCGCTGATGAACTGATGACCGGGTGTACAAATTTTGCGTTCAGCGGAGAACCTGGTACCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGCCAGACAGTGATTGTGGTTACCGTgg                                                                                                        <  2:74751/140‑1 (MQ=255)                      aTCCATCGCTGATGAACTGATGACCGGGTGTACAAATTTTGCGTTCAGCGGAAAACCTGGTACCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGCCAGACAGTGATTGTGGTTACCGTgg                                                                                                        <  1:73709/140‑1 (MQ=255)                      aTCCATCGCTGATGAACTGATGACCGGGTGTACAAATTTTGCGTTCAGCGGAAAACCTGGTACCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGCCAGACAGTGATTGTGGTTACCGTgg                                                                                                        <  1:621142/140‑1 (MQ=255)                           tCGCTGATGAACTGATGACCGGGTGTACAAATTTTGCGTTCAGCGGAAAACCTGGTACCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGCCAGACAGTGATTGTGGTTACCGTGGCTGAt                                                                                                   <  2:422893/140‑1 (MQ=255)                                        gatgaCCGGGTGTACAAATTTTGCGTTCAGCGGAAAACCTGGTACCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGCCAGACAGTGATTGTGGTTACCGTGGCTGATGTTAt                                                                                              >  2:367604/1‑132 (MQ=255)                                        gatgaCCGGGTGTACAAATTTTGCGTTCAGCGGAAAACCTGGTACCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGCCAGACAGTGATTGTGGTTACCGTGGCTGATGTTAt                                                                                              <  1:367604/132‑1 (MQ=255)                                              cGGGTGTACAAATTTTGCGTTCAGCGGAAAACCTGGTACCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGCCAGACAGTGATTGTGGTTACCGTGGCTGATGTTATGAGTGCCCTGCACg                                                                                <  1:78666/140‑1 (MQ=255)                                              cGGGTGTACAAATTTTGCGTTCAGCGGAAAACCTGGTACCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGCCAGACAGTGATTGTGGTTACCGTGGCTGATGTTATGAGTGCCCTGCACg                                                                                <  2:609349/140‑1 (MQ=255)                                                ggTGTACAAATTTTGCGTTCAGCGGAAAACCTGGTACCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGCCAGACAGTGATTGTGGTTACCGTGGCTGATGTTATGAGTGCCCTGCACGcc                                                                              >  1:623773/1‑140 (MQ=255)                                                    tACAAATTTTGCGTTCAGCGGAAAACCTGGTACCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGCCAGACAGTGATTGTGGTTACCGTGGCTGATGTTATGAGTGCCCTGCACGcc                                                                              <  1:22746/136‑1 (MQ=255)                                                    tACAAATTTTGCGTTCAGCGGAAAACCTGGTACCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGCCAGACAGTGATTGTGGTTACCGTGGCTGATGTTATGAGTGCCCTGCACGcc                                                                              >  2:22746/1‑136 (MQ=255)                                                          ttttGCGTTCAGCGGAAAACCTGGTACCGGGAAGAACCACTTAGC‑‑CAGCTATCGGGAATCGCCTGCTGAAAGACGGCCAGACAGTGATTGTGGTTACCGTGGCTGATGTTATGAGTGCCCTGCACGCCAGCTATGACGAt                                                                  >  1:2938/1‑140 (MQ=255)                                                                                ggTACCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGCCAGACAGTGATTGTGGTTACCGTGGCTGATGTTATGAGTGCCCTGCACGCCAGCTATGACGATGGGCAGTCAGGCGAAAAAtt                                              <  2:623773/140‑1 (MQ=255)                                                                                       ggAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGCCAGACAGTGATTGTGGTTACCGTGGCTGATGTTATGAGTGCCCTGCACGCCAGCTATGACGATGGGCAGTCAGGCGAAAAATTTTTGCgg                                       >  2:479202/1‑140 (MQ=255)                                                                                          agaaCCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGCCAGACAGTGATTGTGGTTACCGTGGCTGATGTTATGAGTGCCCTGCACGCCAGCTATGACGATGGGCAGTCAGGCGAAAAATTTTTGCGGGaa                                    <  1:641794/140‑1 (MQ=255)                                                                                           gaaCCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGCCAGACAGTGATTGTGGTTACCGTGGCTGATGTTATGAGTGCCCTGCACGCCAGCTATGACGATGGGCAGTCAGGCGAAAAATTTTTGCGGGAAc                                   <  1:864475/140‑1 (MQ=255)                                                                                                  acttagccAGCTATCGGGAATCGCCTGCTGAAAGACGGCCAGACAGTGATTGTGGTTACCGTGGCTGATGTTATGAGTGCCCTGCACGCCAGCTATGACGATGGGCAGTCAGGCGAAAAATTTTTGCGGGAACTGTGCGa                            <  2:2938/133‑1 (MQ=255)                                                                                                      cGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGCCAGACAGTGATTGTGGTTACCGTGGCTGATGTTATGAGTGCCCTGCACGCCAGCTATGACGATGGGCAGTCAGGCGAAAAATTTTTGCGGGAACTGTGCGAAGTg                        <  2:657915/140‑1 (MQ=255)                                                                                                         cAGCTATCGGGAATCGCCTGCTGAAAGACGGCCAGACAGTGATTGTGGTTACCGTGGCTGATGTTATGAGTGCCCTGCACGCCAGCTATGACGATGGGCAGTCAGGCGAAAAATTTTTGCGGGAACTGTGCGAAGTGGAt                     >  2:292779/1‑140 (MQ=255)                                                                                                           gCTATCGGGAATCGCCTGCTGAAAGACGGCCAGACAGTGATTGTGGTTACCGTGGCTGATGTTATGAGTGCCCTGCACGCCAGCTATGACGATGGGCAGTCAGGCGAAAAATTTTTGCGGGAACTGTGCGAAGTGGATct                   >  1:369394/1‑140 (MQ=255)                                                                                                              aTCGGGAATCGCCTGCTGAAAGACGGCCAGACAGTGATTGTGGTTACCGTGGCTGATGTTATGAGTGCCCTGCACGCCAGCTATGACGATGGGCAGTCAGGCGAAAAATTTTTGCGGGAACTGTGCGAAGTgg                       <  2:706379/133‑1 (MQ=255)                                                                                                              aTCGGGAATCGCCTGCTGAAAGACGGCCAGACAGTGATTGTGGTTACCGTGGCTGATGTTATGAGTGCCCTGCACGCCAGCTATGACGATGGGCAGTCAGGCGAAAAATTTTTGCGGGAACTGTGCGAAGTgg                       >  1:706379/1‑133 (MQ=255)                                                                                                                         ccTGCTGAAAGACGGCCAGACAGTGATTGTGGTTACCGTGGCTGATGTTATGAGTGCCCTGCACGCCAGCTATGACGATGGGCAGTCAGGCGAAAAATTTTTGCGGGAACTGTGCGAAGTGGATCTGCTGGTTCTtgatg     >  1:153700/1‑140 (MQ=255)                                                                                                                          ctgctgAAAGACGGCCAGACAGTGATTGTGGTTACCGTGGCTGATGTTATGAGTGCCCTGCACGCCAGCTATGACGATGGGCAGTCAGGCGAAAAATTTTTGCGGGAACTGTGCGAAGTGGATCTGCTGGTTCTtgatga    <  2:153700/140‑1 (MQ=255)                                                                                                                            gctgAAAGACGGCCAGACAGTGATTGTGGTTACCGTGGCTGATGTTATGAGTGCCCTGCACGCCAGCTATGACGATGGGCAGTCAGGCGAAAAATTTTTGCGGGAACTGTGCGAAGTGGATCTGCTGGTTCTTGATGaaa  >  1:784537/1‑140 (MQ=255)                                                                                                                                  aGACGGCCAGACAGTGATTGTGGTTACCGTGGCTGATGTTATGAGTGCCCTGCACGCCAGCTATGACGATGGGCAGTCAGGCGAAAAATTTTTGCGGGAACTGTGCGAAGTGGATCTGCTGGTTCTtgatga    >  2:546168/1‑132 (MQ=255)                                                                                                                                  aGACGGCCAGACAGTGATTGTGGTTACCGTGGCTGATGTTATGAGTGCCCTGCACGCCAGCTATGACGATGGGCAGTCAGGCGAAAAATTTTTGCGGGAACTGTGCGAAGTGGATCTGCTGGTTCTtgatga    <  1:546168/132‑1 (MQ=255)                                                                                                                                        |                                                                                                                                ATACGCGTTGAGTCAGGCGAAATCCATCGCTGATGAACTGATGACCGGGTGTACAAATTTTGCGTTCAGCGGAAAACCTGGTACCGGGAAGAACCACTTAGCGGCAGCTATCGGGAATCGCCTGCTGAAAGACGGTCAGACAGTGATTGTGGTTACCGTGGCTGATGTTATGAGTGCCCTGCACGCCAGCTATGACGATGGGCAGTCAGGCGAAAAATTTTTGCGGGAACTGTGCGAAGTGGATCTGCTGGTTCTTGATGAAA  >  NC_000913/1422234‑1422496

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑