Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,565,109	G→A	Q209* (CAG→TAG)	dosP ←	oxygen‑sensing c‑di‑GMP phosphodiesterase DosP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,565,109	0	G	A	100.0%	120.6 / NA	35	Q209* (CAG→TAG)	dosP	oxygen‑sensing c‑di‑GMP phosphodiesterase DosP
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/18);  total (17/18)

CGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAATTCAGGAATGTTCAGGAGTGTATCGGGCTGCATACCGCTGGCTTCGCTAATGCAGTAACCAAACATTTCGGTAAATGCG  >  NC_000913/1564979‑1565245                                                                                                                                   |                                                                                                                                         cGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTAATCgcgggc                                                                                                                                 >  1:534698/1‑140 (MQ=255)             atatCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTC‑ACAGCAGAAATTCGTCCTAATCGCGGGCGGTTTTCCATAGc                                                                                                                    <  2:169508/140‑1 (MQ=255)                tCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTAATCGCGGGCGGTTTTCCATAGCaa                                                                                                                  >  2:670162/1‑140 (MQ=255)                         gTCATTACCAGGTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTAATCGCGGGCGGTTTTCCATAGCAACTGTTGTaa                                                                                                         >  1:584918/1‑140 (MQ=255)                                ccAGGTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTAATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATg                                                                                                  <  1:670162/140‑1 (MQ=255)                                        tGCAGATGCGCGAGCACGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTAATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATc                                                                                          >  1:754993/1‑140 (MQ=255)                                        tGCAGATGCGCGAGCACGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTAATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATc                                                                                          >  1:44444/1‑140 (MQ=255)                                        tGCAGATGCGCGAGCACGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTAATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATc                                                                                          >  1:56764/1‑140 (MQ=255)                                            gATGCGCGAGCACGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTAATCGCGGTCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCa                                                                                      <  2:739572/140‑1 (MQ=255)                                            gATGCGCGAGCACGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTAATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCa                                                                                      <  2:754993/140‑1 (MQ=255)                                              tGCGCGAGCACGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTAATCGCGGGTGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAgg                                                                                    >  2:214050/1‑140 (MQ=255)                                               gcgcGAGCACGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTAATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAggg                                                                                   >  1:1065/1‑140 (MQ=255)                                               gcgcGAGCACGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTAATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAggg                                                                                   >  1:860601/1‑140 (MQ=255)                                                  cgAGCACGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTAATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAAt                                                                                >  1:487673/1‑140 (MQ=255)                                                  cgAGCACGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTAATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAAt                                                                                >  1:441568/1‑140 (MQ=255)                                                      cACGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTAATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAATTCAg                                                                            <  1:293790/140‑1 (MQ=255)                                                        cGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTAATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAATTCAGGa                                                                          <  2:487673/140‑1 (MQ=255)                                                         gTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTAATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAATTCAGGa                                                                          >  1:605573/1‑139 (MQ=255)                                                         gTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTAATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAATTCAGGa                                                                          <  2:605573/139‑1 (MQ=255)                                                             tAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTAATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAATTCAGGAATGtt                                                                     <  2:56764/140‑1 (MQ=255)                                                                    ggCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTAATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAATTCAGGAATGTTCAGGAgt                                                              >  1:474029/1‑140 (MQ=255)                                                                            gagGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTAATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAATTCAGGAATGTTCAGGAGTGTATCggg                                                      <  1:295468/140‑1 (MQ=255)                                                                            gagGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTAATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAATTCAGGAATGTTCAGGAGTGTATCggg                                                      <  2:474029/140‑1 (MQ=255)                                                                               gCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTAATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAATTCAGGAATGTTCAGGAGTGTATCGGGCTg                                                   >  1:363829/1‑140 (MQ=255)                                                                                 tttAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTAATCGCGGGTGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAATTCAGGAATGTTCAGGAGTGTATCGGGCTGCa                                                 <  1:214050/140‑1 (MQ=255)                                                                                   tAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTAATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAATTCAGGAATGTTCAGGAGTGTATCGGGCTGCATa                                               <  2:363829/140‑1 (MQ=255)                                                                                      tCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTAATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAATTCAGGAATGTTCAGGAGTGTATCGGGCTGCATACCg                                            <  1:302773/140‑1 (MQ=255)                                                                                         aGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTAATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAATTCAGGAATGTTCAGGAGTGTATCGGGCTGCATACCGCTg                                         >  2:501406/1‑140 (MQ=255)                                                                                          gATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTAATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAATTCAGGAATGTTCAGGAGTGTATCGGGCTGCATACCGCttg                                        <  2:534698/140‑3 (MQ=255)                                                                                          gATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTAATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAATTCAGGAATGTTCAGGAGTGTATCGGGCTGCATACCGCTgg                                        >  1:529934/1‑140 (MQ=255)                                                                                             tttttCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTAATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAATTCAGGAATGTTCAGGAGTGTATCGGGCTGCATACCGCTGGCtt                                     <  2:441568/140‑1 (MQ=255)                                                                                                                     cagAAATTCGTCCTAATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAATTCAGGAATGTTCAGGAGTGTATCGGGCTGCATACCGCTGGCTTCGCTAATGCAGTAACCAAACAttt             <  2:529934/140‑1 (MQ=255)                                                                                                                             cGTCCTAATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAATTCAGGAATGTTCAGGAGTGTATCGGGCTGCATACCGCTGGCTTCGCTAATGCAGTAACCAAACATTTCGGTAAAt     >  2:255385/1‑140 (MQ=255)                                                                                                                              gTCCTAATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAATTCAGGAATGTTCAGGAGTGTATCGGGCTGCATACCGCTGGCTTCGCTAATGCAGTAACCAAACATTTCGGTAAATg    <  2:584918/140‑1 (MQ=255)                                                                                                                                ccTAATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAATTCAGGAATGTTCAGGAGTGTATCGGGCTGCATACCGCTGGCTTCGCTAATGCAGTAACCAAACATTTCGGTAAATgcg  <  1:501406/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                         CGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAATTCAGGAATGTTCAGGAGTGTATCGGGCTGCATACCGCTGGCTTCGCTAATGCAGTAACCAAACATTTCGGTAAATGCG  >  NC_000913/1564979‑1565245

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑