Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	175,133	A→G	E9E (GAA→GAG)	clcA →	chloride:H(+) antiporter ClcA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	175,133	0	A	G	100.0%	125.5 / NA	35	E9E (GAA→GAG)	clcA	chloride:H(+) antiporter ClcA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/18);  total (17/18)

TATAAAAGTTTTGTGCATTTGAAACATTACGCTTTGCAAAGGATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGCTTTTG  >  NC_000913/175000‑175266                                                                                                                                      |                                                                                                                                      tataAAAGTTTTGTGCATTTGAAACATTACGCTTTGCAAAGGATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAGACACCg                                                                                                                                 <  1:573017/140‑1 (MQ=255)           ttGTGCATTTGAAACATTACGCTTTGCAAAGGATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAGACACCGCAGGCcgcgc                                                                                                                       >  1:257977/1‑140 (MQ=255)           ttGTGCATTTGAAACATTACGCTTTGCAAAGGATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAGACACCGCAGGCcgcgc                                                                                                                       >  1:60433/1‑140 (MQ=255)                   ttGAAACATTACGCTTTGCAAAGGATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAGACACCGCAGGCCGCGCGCCTGCGa                                                                                                               <  2:340118/140‑1 (MQ=255)                     gAAACATTACGCTTTGCAAAGGATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAGACACCGCAGGCCGCGCGCCTGCGACg                                                                                                             <  1:178918/140‑1 (MQ=255)                             aCGCTTTGCAAAGGATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAGACACCGCAGGCCGCGCGCCTGCGACGCAGACAAc                                                                                                     >  1:210097/1‑140 (MQ=255)                                 tttGCAAAGGATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAGACACCGCAGGCCGCGCGCCTGCGACGCAGACACCTGAt                                                                                                 >  1:805067/1‑140 (MQ=255)                                 tttGCAAAGGATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAGACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGAt                                                                                                 <  2:497297/140‑1 (MQ=255)                                   tGCAAAGGATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAGACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTc                                                                                               >  2:81512/1‑140 (MQ=255)                                   tGCAAAGGATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAGACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTc                                                                                               >  2:217481/1‑140 (MQ=255)                                    gCAAAGGATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAGACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCg                                                                                              >  1:273276/1‑140 (MQ=255)                                     cAAAGGATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAGACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGc                                                                                             >  1:479965/1‑140 (MQ=255)                                      aaaGGATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAGACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGcc                                                                                            <  1:217481/140‑1 (MQ=255)                                             tttCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAGACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAActtct                                                                                     >  1:675037/1‑140 (MQ=255)                                             tttCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAGACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAActtct                                                                                     >  1:248480/1‑140 (MQ=255)                                                  tGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAGACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAgc                                                                                <  2:210097/140‑1 (MQ=255)                                                  tGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAGACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAgc                                                                                <  2:28169/140‑1 (MQ=255)                                                   ggAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAGACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAgcg                                                                               <  2:60433/140‑1 (MQ=255)                                                   ggAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAGACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAgcg                                                                               <  1:81512/140‑1 (MQ=255)                                                   ggAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAGACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAgcg                                                                               <  2:257977/140‑1 (MQ=255)                                                   ggAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAGACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAgcg                                                                               <  2:77394/140‑1 (MQ=255)                                                    gAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAGACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGcc                                                                                            >  1:811290/1‑126 (MQ=255)                                                    gAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAGACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGcc                                                                                            <  2:811290/126‑1 (MQ=255)                                                               tAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAGACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAAcccc                                                                   >  1:422338/1‑140 (MQ=255)                                                                 aaaTGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAGACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGt                                                                 <  2:479965/140‑1 (MQ=255)                                                                                      aTGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAGACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATggcgg                                            <  2:430321/140‑1 (MQ=255)                                                                                       tGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAGACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATggcggc                                           >  1:40501/1‑140 (MQ=255)                                                                                          ccAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAGACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATg                                                >  2:769694/1‑132 (MQ=255)                                                                                          ccAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAGACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATg                                                <  1:769694/132‑1 (MQ=255)                                                                                             aTAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAGACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAgtcgt                                     <  2:40501/140‑1 (MQ=255)                                                                                                       aTTGGATGAAAACTGATACTCCCTCTTTAGAGACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCtt                           >  2:372579/1‑140 (MQ=255)                                                                                                               aaaaCTGATACTCCCTCTTTAGAGACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCTTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCt                   <  2:273276/140‑1 (MQ=255)                                                                                                                   cTGATACTCCCTCTTTAGAGACACCGCAGGCCGCGCGCCTGCGGCGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCa               >  1:528270/1‑140 (MQ=255)                                                                                                                       tACTCCCTCTTTAGAGACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCgg           <  2:675037/140‑1 (MQ=255)                                                                                                                                tttAGAGACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGCTTTTg  >  1:335601/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                      TATAAAAGTTTTGTGCATTTGAAACATTACGCTTTGCAAAGGATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGCTTTTG  >  NC_000913/175000‑175266

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑