Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,606,759	A→G	V220V (GTA→GTG)	lsrF →	3‑hydroxy‑2,4‑pentadione 5‑phosphate thiolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,606,759	0	A	G	100.0%	133.2 / NA	37	V220V (GTA→GTG)	lsrF	3‑hydroxy‑2,4‑pentadione 5‑phosphate thiolase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/22);  total (15/22)

GGCGTGGGCAAAGATATGGTGCGCGATCAGCGTTATTTCTCGCTCGCGACTCGAATCGCCGCTGAAATGGGGGCGCAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGTACCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCAGGCTATCGATCAGGGCGCTTCTGGTGTGGATATGGGGCGTAATATTTTCCAGTCTGACCATCCGGTGGCG  >  NC_000913/1606622‑1606891                                                                                                                                          |                                                                                                                                     ggCGTGGGCAAAGATATGGTGCGCGATCAGCGTTATTTCTCGCTCGCGACTCGAATCGCCGCTGAAATGGGGGCGCAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGTGcc                                                                                                                                    <  2:435720/140‑1 (MQ=255)  gCGTGGGCAAAGATATGGTGCGCGATCAGCGTTATTTCTCGCTCGCGACTCGAATCGCCGCTGAAATGGGGGCGCAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGTGccc                                                                                                                                   <  2:213859/140‑1 (MQ=255)  gCGTGGGCAAAGATATGGTGCGCGATCAGCGTTATTTCTCGCTCGCGACTCGAATCGCCGCTGAAATGGGGGCGCAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGTGccc                                                                                                                                   >  2:178880/1‑140 (MQ=255)   cGTGGGCAAAGATATGGTGCGCGATCAGCGTTATTTCTCGCTCGCGACTCGAATCGCCGCTGAAATGGGGGCGCAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGTGCCCa                                                                                                                                  >  1:587122/1‑140 (MQ=255)   cGTGGGCAAAGATATGGTGCGCGATCAGCGTTATTTCTCGCTCGCGACTCCAATCGCCGCTGAAATGGGGGCGCAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGTGCCCa                                                                                                                                  <  2:556511/140‑1 (MQ=255)       ggCAAAGATATGGTGCGCGATCAGCGTTATTTCTCGCTCGCGACTCGAATCGCCGCTGAAATGGGGGCGCAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGTGCCCATTGt                                                                                                                              >  1:366768/1‑140 (MQ=255)            aGATATGGTGCGCGATCAGCGTTATTTCTCGCTCGCGACTCGAATCGCCGCTGAAATGGGGGCGCAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGTGCCCATTGTTATTg                                                                                                                         >  1:72350/1‑140 (MQ=255)             gATATGGTGCGCGATCAGCGTTATTTCTCGCTCGCGACTCGAATCGCCGCTGAAATGGGGGCGCAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGTGCCCATTGTTATTGc                                                                                                                        <  1:706235/140‑1 (MQ=255)                      cgcgATCAGCGTTATTTCTCGCTCGCGACTCGAATCGCCGCTGAAATGGGGGCGCAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGTGCCCATTGTTATTGCTGGCGGTaa                                                                                                               >  1:624906/1‑140 (MQ=255)                       gcgATCAGCGTTATTTCTCGCTCGCGACTCGAATCGCCGCTGAAATGGGGGCGCAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGTGCCCATTGTTATTGCTGGCGGCaaa                                                                                                              >  1:603692/1‑140 (MQ=255)                                       ctcgctcgCGACTCGAATCGCCGCTGAAATGGGGGCGCAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGTGCCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGcgcg                                                                                              <  2:624906/140‑1 (MQ=255)                                        tcgctcgcGACTCGAATCGCCGCTGAAATGGGGGCGCAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGTGCCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGa                                                                                             <  2:864424/140‑1 (MQ=255)                                        tcgctcgcGACTCGAATCGCCGCTGAAATGGGGGCGCAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGTGCCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGa                                                                                             <  2:643582/140‑1 (MQ=255)                                         cgctcgcGACTCGAATCGCCGCTGAAATGGGGGCGCAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGTGCCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAg                                                                                            >  1:818621/1‑140 (MQ=255)                                                   tCGAATCGCCGCTGAAATGGGGGCGCAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGTGCCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGaaa                                                                                  >  1:446624/1‑140 (MQ=255)                                                      aaTCGCCGCTGAAATGGGGGCGCAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGTGCCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAAtgt                                                                               >  1:800462/1‑140 (MQ=255)                                                            cgcTGAAATGGGGGCGCAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGTGCCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGc                                                                         >  2:662135/1‑140 (MQ=255)                                                                 taaTTGGGGCGCAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGAATGTCTGGTGCCCAGTGTTATTGCTTGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCAGGCt                                                                    <  2:458251/139‑1 (MQ=255)                                                                      ggggCGCAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGTGCCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCAGGCTATCGa                                                               >  2:437829/1‑140 (MQ=255)                                                                            cAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGTGCCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCAGGCTATCGATCAggg                                                         <  1:666909/140‑1 (MQ=255)                                                                            cAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGTGCCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCAGGCTATCGATCAggg                                                         <  2:72350/140‑1 (MQ=255)                                                                            cAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGTGCCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCAGGCTATCGATCAggg                                                         <  2:603692/140‑1 (MQ=255)                                                                                ttATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGTGCCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCAGGCTATCGATCAGGGCGCt                                                     <  2:800462/140‑1 (MQ=255)                                                                                     aaaaCCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGTGCCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCAGGCTATCGATCAGGGCGCTTCTgg                                                <  2:398728/140‑1 (MQ=255)                                                                                       aaCCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGTGCCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCAGGCTATCGATCAGGGCGCTTCTGgtg                                              <  2:311543/140‑1 (MQ=255)                                                                                       aaCCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGTGCCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCAGGCTATCGATCAGGGCGCTTCTGgtg                                              >  1:144239/1‑140 (MQ=255)                                                                                       aaCCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGTGCCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCAGGCTATCGATCAGGGCGCTTCTGgtg                                              <  1:282770/140‑1 (MQ=255)                                                                                            attatGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGTGCCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCAGGCTATCGATCAGGGCGCTTCTGGTGTGGat                                         >  1:102140/1‑140 (MQ=255)                                                                                                   cGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGTGCCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCAGGCTATCGATCAGGGCGCTTCTGGTGTGGATATGGGGc                                  <  2:782791/140‑1 (MQ=255)                                                                                                   cGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGTGCCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCAGGCTATCGATCAGGGCGCTTCTGGTGTGGATATGGGGc                                  >  2:104625/1‑140 (MQ=255)                                                                                                         aGGTTTTGAACGGATTGTTGCCGGATGTCCGGTGCCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCAGGCTATCGATCAGGGCGCTTCTGGTGTGGATATGGGGCGTAata                            <  1:145029/140‑1 (MQ=255)                                                                                                           gTTTTGAACGGATTGTTGCCGGATGTCCGGTGCCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCAGGCTATCGATCAGGGCGCTTCTGGTGTGGATATGGGGCGTAATAtt                          >  1:417172/1‑140 (MQ=255)                                                                                                            ttttGAACGGATTGTTGCCGGATGTCCGGTGCCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCAGGCTATCGATCAGGGCGCTTCTGGTGTGGATATGGGGCGTAATAttt                         <  2:144239/140‑1 (MQ=255)                                                                                                             tttGAACGGATTGTTGCCGGATGTCCGGTGCCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCAGGCTATCGATCAGGGCGCTTCTGGTGTGGATATGGGGCGTAATAtttt                        <  2:366768/140‑1 (MQ=255)                                                                                                                           tgCCGGATGTCCGGTGCCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCAGGCTATCGATCAGGGCGCTTCTGGTGTGGATATGGGGCGTAATATTTTCCAGTCTGACCATc          <  1:662135/140‑1 (MQ=255)                                                                                                                                gATGTCCGGTGCCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCAGGCTATCGATCAGGGCGCTTCTGGTGTGGATATGGGGCGTAATATTTTCCAGTCTGACCATCCGGTg     <  1:437829/140‑1 (MQ=255)                                                                                                                                 aTGTCCGGTGCCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCAGGCTATCGATCAGGGCGCTTCTGGTGTGGATATGGGGCGTAATATTTTCCAGTCTGACCATCCGGTgg    <  2:587122/140‑1 (MQ=255)                                                                                                                                   gTCCGGTGCCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCAGGCTATCGATCAGGGCGCTTCTGGTGTGGATATGGGGCGTAATATTTTCCAGTCTGACCATCCGGTGGCg  <  2:446624/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                     GGCGTGGGCAAAGATATGGTGCGCGATCAGCGTTATTTCTCGCTCGCGACTCGAATCGCCGCTGAAATGGGGGCGCAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGTACCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCAGGCTATCGATCAGGGCGCTTCTGGTGTGGATATGGGGCGTAATATTTTCCAGTCTGACCATCCGGTGGCG  >  NC_000913/1606622‑1606891

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑