Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,613,520	G→A	A395V (GCG→GTG)	sad ←	NAD(+)‑dependent succinate semialdehyde dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,613,520	0	G	A	100.0%	148.3 / NA	43	A395V (GCG→GTG)	sad	NAD(+)‑dependent succinate semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/24);  total (19/24)

GCCACTCTTTTTCACGCCACCAAAGGCCACTCGCGCGTCGCTGGCACAATAACCATTGATAAACACCCCACCGCATTCCAGACGTGCCGCCATCTGTCTGGCCTGTGTTTCGTCAGTGGTAAAAATGGTCGCTGAAAGGCCGAACTCACTATCATTAGCCAGTTCCAGTGCATGTTCTGCATCTTTCGCAATGGTGATTGCCGCAACGGGGCCAAACATTTCTTCCCGAAACGCGGTCATTTCTGGGGTAACATTCGCCAGAACCG  >  NC_000913/1613390‑1613655                                                                                                                                   |                                                                                                                                        gCCACTCTTTTTCACGCCACCAAAGGCCACTCGCGCGTCGCTGGCACAATAACCATTGATAAACACCCCACCGCATTCCAGACGTGCCGCCATCTGTCTGGCCTGTGTTTCGTCAGTGGTAAAAATGGTCACTGAAAGGc                                                                                                                                <  1:343993/140‑1 (MQ=255)  ccACTCTTTTTCACGCCACCAAAGGCCACTCGCGCGTCGCTGGCACAATAACCATTGATAAACACCCCACCGCATTCCAGACGTGCCGCCATCTGTCTGGCCTGTGTTTCGTCAGTGGTAAAAATGGTCACTGAAAGGcc                                                                                                                               <  2:334047/140‑1 (MQ=255)   cACTCTTTCTCACGCCACCAAAGGCCACTCGCGCGTCGCTGGCACAATAACCATTGATAAACACCCCACCGCATTCCAGACGTGCCGCCATCTGTCTGGCCTGTGTTTCGTCAGTGGTAAAAATGGTCACTGAAAGGCCg                                                                                                                              <  2:112149/140‑1 (MQ=255)    aCTCTTTTTCACGCCACCAAAGGCCACTCGCGCGTCGCTGGCACAATAACCATTGATAAACACCCCACCGCATTCCAGACGTGCCGCCATCTGTCTGGCCTGTGTTTCGTCAGTGGTAAAAATGGTCACTGAAAGGCCGa                                                                                                                             <  1:579644/140‑1 (MQ=255)     ctctTTTTCACGCCACCAAAGGCCACTCGCGCGTCGCTGGCACAATAACCATTGATAAACACCCCACCGCATTCCAGACGTGCCGCCATCTGTCTGGCCTGTGTTTCGTCAGTGGTAAAAATGGTCACTGAAAGGCCGaa                                                                                                                            <  1:225697/140‑1 (MQ=255)     ctctTTTTCACGCCACCAAAGGCCACTCGCGCGTCGCTGGCACAATAACCATTGATAAACACCCCACCGCATTCCAGACGTGCCGCCATCTGTCTGGCCTGTGTTTCGTCAGTGGTAAAAATGGTCACTGAAAGGCCGaa                                                                                                                            <  1:368469/140‑1 (MQ=255)      tctTTTTCACGCCACCAAAGGCCACTCGCGCGTCGCTGGCACAATAACCATTGATAAACACCCCACCGCATTCCAGACGTGCCGCCATCTGTCTGGCCTGTGTTTCGTCAGTGGTAAAAATGGTCACTGAAAGGCCGAAc                                                                                                                           >  2:222333/1‑140 (MQ=255)          tttCACGCCACCAAAGGCCACTCGCGCGTCGCTGGCACAATAACCATTGATAAACACCCCACCGCATTCCAGACGTGCCGCCATCTGTCTGGCCTGTGTTTCGTCAGTGGTAAAAATGGTCACTGAAAGGCCGAACTCAc                                                                                                                       <  2:75197/140‑1 (MQ=255)          tttCACGCCACCAAAGGCCACTCGCGCGTCGCTGGCACAATAACCATTGATAAACACCCCACCGCATTCCAGACGTGCCGCCATCTGTCTGGCCTGTGTTTCGTCAGTGGTAAAAATGGTCACTGAAAGGCCGAACTCAc                                                                                                                       <  2:733593/140‑1 (MQ=255)          tttCACGCCACCAAAGGCCACTCGCGCGTCGCTGGCACAATAACCATTGATAAACACCCCACCGCATTCCAGACGTGCCGCCATCTGTCTGGCCTGTGTTTCGTCAGTGGTAAAAATGGTCACTGAAAGGCCGAACTCAc                                                                                                                       <  1:359140/140‑1 (MQ=255)          tttCACGCCACCAAAGGCCACTCGCGCGTCGCTGGCACAATAACCATTGATAAACACCCCACCGCATTCCAGACGTGCCGCCATCTGTCTGGCCTGTGTTTCGTCAGTGGTAAAAATGGTCACTGAAAGGCCGAACTCAc                                                                                                                       <  1:494035/140‑1 (MQ=255)                       aaGGCCACTCGCGCGTCGCTGGCACAATAACCATTGATAAACACCCCACCGCATTCCAGACGTGCCGCCATCTGTCTGGCCTGTGTTTCGTCAGTGGTAAAAATGGTCACTGAAAGGCCGAACTCACTATCATTAGCCAg                                                                                                          <  1:222333/140‑1 (MQ=255)                         ggCCACTCGCGCGTCGCTGGCACAATAACCATTGATAAACACCCCACCGCATTCCAGACGTGCCGCCATCTGTCTGGCCTGTGTTTCGTCAGTGGTAAAAATGGTCACTGAAAGGCCGAACTCACTATCATTAGCCAGtt                                                                                                        <  1:315949/140‑1 (MQ=255)                            cACTCGCGCGTCGCTGGCACAATAACCATTGATAAACACCCCACCGCATTCCAGACGTGCCGCCATCTGTCTGGCCTGTGTTTCGTCAGTGGTAAAAATGGTCACTGAAAGGCCGAACTCACTATCATTa                                                                                                               <  2:850942/130‑1 (MQ=255)                            cACTCGCGCGTCGCTGGCACAATAACCATTGATAAACACCCCACCGCATTCCAGACGTGCCGCCATCTGTCTGGCCTGTGTTTCGTCAGTGGTAAAAATGGTCACTGAAAGGCCGAACTCACTATCATTa                                                                                                               >  1:850942/1‑130 (MQ=255)                              cTCGCGCGTCGCTGGCACAATAACCATTGATAAACACCCCACCGCATTCCAGACGTGCCGCCATCTGTCTGGCCTGTGTTTCGTCAGTGGTAAAAATGGTCACTGAAAGGCCGAACTCACTATCATTAGCCAGTTCCAGt                                                                                                   >  1:31650/1‑140 (MQ=255)                               tCGCGCGTCGCTGGCACAATAACCATTGATAAACACCCCACCGCATTCCAGACGTGCCGCCATCTGTCTGGCCTGTGTTTCGTCAGTGGTAAAAATGGTCACTGAAAGGCCGAACTCACTATCATTAGCCAGTTCCAGTg                                                                                                  >  2:861080/1‑140 (MQ=255)                                cgcgcgTCGCTGGCACAATAACCATTGATAAACACCCCACCGCATTCCAGACGTGCCGCCATCTGTCTGGCCTGTGTTTCGTCAGTGGTAAAAATGGTCACTGAAAGGCCGAACTCACTATCATTAGCCAGTTCCAGTGc                                                                                                 <  2:31650/140‑1 (MQ=255)                                    cgTCGCTGGCACAATAACCATTGATAAACACCCCACCGCATTCCAGACGTGCCGCCATCTGTCTGGCCTGTGTTTCGTCAGTGGTAAAAATGGTCACTGAAAGGCCGAACTCACTATCATTAGCCAGTTCCAGTGCATGt                                                                                             >  1:596205/1‑140 (MQ=255)                                                        ttGATAAACACCCCACCGCATTCCAGACGTGCCGCCATCTGTCTGGCCTGTGTTTCGTCAGTGGTAAAAATGGTCACTGAAAGGCCGAACTCACTATCATTAGCCAGTTCCAGTGCATGTTCTGCATCTTTCGCAATGGt                                                                         >  2:312908/1‑140 (MQ=255)                                                                cacCCCACCGCATTCCAGACGTGCCGCCATCTGTCTGGCCTGTGTTTCGTCAGTGGTAAAAATGGTCACTGAAAGGCCGAACTCACTATCATTAGCCAGTTCCAGTGCATGTTCTGCATCTTTCGCAATGGTGATTGCCg                                                                 >  2:443174/1‑140 (MQ=255)                                                                   cccACCGCATTCCAGACGTGCCGCCATCTGTCTGGCCTGTGTTTCGTCAGTGGTAAAAATGGTCACTGAAAGGCCGAACTCACTATCATTAGCCAGTTCCAGTGCATGTTCTGCATCTTTCGCAATGGTGATTGCCGCaa                                                              >  2:615145/1‑140 (MQ=255)                                                                    ccACCGCATTCCAGACGTGCCGCCATCTGTCTGGCCTGTGTTTCGTCAGTGGTAAAAATGGTCACTGAAAGGCCGAACTCACTATCATTAGCCAGTTCCAGTGCATGTTCTGCATCTTTCGCAATGGTGATTGCCGCAAc                                                             <  2:395666/140‑1 (MQ=255)                                                                     cACCGCATTCCAGACGTGCCGCCATCTGTCTGGCCTGTGTTTCGTCAGTGGTAAAAATGGTCACTGAAAGGCCGAACTCACTATCATTAGCCAGTTCCAGTGCATGTTCTGCATCTTTCGCAATGGTGATTGCCGCAACg                                                            >  1:589393/1‑140 (MQ=255)                                                                      aCCGCATTCCAGACGTGCCGCCATCTGTCTGGCCTGTGTTTCGTCAGTGGTAAAAATGGTCACTGAAAGGCCGAACTCACTATCATTAGCCAGTTCCAGTGCATGTTCTGCATCTTTCGCAATGGTGATTGCCGCAACgg                                                           >  1:642538/1‑140 (MQ=255)                                                                           aTTCCAGACGTGCCGCCATCTGTCTGGCCTGTGTTTCGTCAGTGGTAAAAATGGTCACTGAAAGGCCGAACTCACTATCATTAGCCAGTTCCAGTGCATGTTCTGCATCTTTCGCAATGGTGATTGCCGCAACGGGGCCa                                                      >  2:266492/1‑140 (MQ=255)                                                                           aTTCCAGACGTGCCGCCATCTGTCTGGCCTGTGTTTCGTCAGTGGTAAAAATGGTCACTGAAAGGCCGAACTCACTATCATTAGCCAGTTCCAGTGCATGTTCTGCATCTTTCGCAATGGTGATTGCCGCAACGGGGCCa                                                      <  1:443174/140‑1 (MQ=255)                                                                                     tGCCGCCATCTGTCTGGCCTGTGTTTCGTCAGTGGTAAAAATGGTCACTGAAAGGCCGAACTCACTATCATTAGCCAGTTCCAGTGCATGTTCTGCATCTTTCGCAATGGTGATTGCCGCAACgggg                                                         >  2:296630/1‑127 (MQ=255)                                                                                     tGCCGCCATCTGTCTGGCCTGTGTTTCGTCAGTGGTAAAAATGGTCACTGAAAGGCCGAACTCACTATCATTAGCCAGTTCCAGTGCATGTTCTGCATCTTTCGCAATGGTGATTGCCGCAACgggg                                                         <  1:296630/127‑1 (MQ=255)                                                                                     tGCCGCCATCTGTCTGGCCTGTGTTTCGTCAGTGGTAAAAATGGTCACTGAAAGGCCGAACTCACTATCATTAGCCAGTTCCAGTGCATGTTCTGCATCTTTCGCAATGGTGATTGCCGCAACGGGGCCAAACATttctt                                            >  1:545868/1‑140 (MQ=255)                                                                                       ccgccATCTGTCTGGCCTGTGTTTCGTCAGTGGTAAAAATGGTCACTGAAAGGCCGAACTCACTATCATTAGCCAGTTCCAGTGCATGTTCTGCATCTTTCGCAATGGTGATTGCCGCAACGGGGCCAAACATTTCTTcc                                          >  2:250118/1‑140 (MQ=255)                                                                                       ccgccATCTGTCTGGCCTGTGTTTCGTCAGTGGTAAAAATGGTCACTGAAAGGCCGAACTCACTATCATTAGCCAGTTCCAGTGCATGTTCTGCATCTTTCGCAATGGTGATTGCCGCAACGGGGCCAAACATTTCTTcc                                          >  2:194602/1‑140 (MQ=255)                                                                                            aTCTGTCTGGCCTGTGTTTCGTCAGTGGTAAAAATGGTCACTGAAAGGCCGAACTCACTATCATTAGCCAGTTCCAGTGCATGTTCTGCATCTTTCGCAATGGTGATTGCCGCAACGGGGCCAAACATTTCTTCCCGaaa                                     <  2:650168/140‑1 (MQ=255)                                                                                                   tgGCCTGTGTTTCGTCAGTGGTAAAAATGGTCACTGAAAGGCCGAACTCACTATCATTAGCCAGTTCCAGTGCATGTTCTGCATCTTTCGCAATGGTGATTGCCGCAACGGGGCCAAACATTTCTTCCCGAAACGCGGTc                              <  2:303746/140‑1 (MQ=255)                                                                                                       cTGTGTTTCGTCAGTGGTAAAAATGGTCACTGAAAGGCCGAACTCACTATCATTAGCCAGTTCCAGTGCATGTTCTGCATCTTTCGCAATGGTGATTGCCGCAACGGGGCCAAACATTTCTTCCCGAAACGCGGTCAttt                          <  1:861080/140‑1 (MQ=255)                                                                                                        tgtgTTTCGTCAGTGGTAAAAATGGTCACTGAAAGGCCGAACTCACTATCATTAGCCAGTTCCAGTGCATGTTCTGCATCTTTCGCAATGGTGATTGCCGCAACGGGGCCAAACATTTCTTCCCGAAACGCGGTCATTTc                         <  1:845389/140‑1 (MQ=255)                                                                                                           gtTTCGTCAGTGGTAAAAATGGTCACTGAAAGGCCGAACTCACTATCATTAGCCAGTTCCAGTGCATGTTCTGCATCTTTCGCAATGGTGATTGCCGCAACGGGGCCAAACATTTCTTCCCGAAACGCGGTCATTTCTgg                      >  2:205403/1‑140 (MQ=255)                                                                                                             ttCGTCAGTGGTAAAAATGGTCACTGAAAGGCCGAACTCACTATCATTAGCCAGTTCCAGTGCATGTTCTGCATCTTTCGCAATGGTGATTGCCGCAACGGGGCCAAACATTTCTTCCCGAAACGCGGTCATTTCTgggg                    <  1:205403/140‑1 (MQ=255)                                                                                                                  cAGTGGTAAAAATGGTCACTGAAAGGCCGAACTCACTATCATTAGCCAGTTCCAGTGCATGTTCTGCATCTTTCGCAATGGTGATTGCCGCAACGGGGCCAAACATTTCTTCCCGAAACGCGGTCATTTCTGGGGTAACa               <  2:256785/140‑1 (MQ=255)                                                                                                                    gTGGTAAAAATGGTCACTGAAAGGCCGAACTCACTATCATTAGCCAGTTCCAGTGCATGTTCTGCATCTTTCGCAATGGTGATTGCCGCAACGGGGCCAAACATTTCTTCCCGAAACGCGGTCATTTCTGGGGTAACAtt             >  2:842663/1‑140 (MQ=255)                                                                                                                        tAAAAATGGTCACTGAGAGGCCGAACTCACTATCATTAGCCAGTTCCAGTGCATGTTCTGCATCTTTCGCAATGGTGATTGCCGCAACGGGGCCAAACATTTCTTCCCGAAACGCGGTCATTTCTGGGGTAACATTCGcc         >  1:301290/1‑140 (MQ=255)                                                                                                                               ggTCACTGAAAGGCCGAACTCACTATCATTAGCCAGTTCCAGTGCATGTTCTGCATCTTTCGCAATGGTGATTGCCGCAACGGGGCCAAACATTTCTTCCCGAAACGCGGTCATTTCTGGGGTAACATTCGCCAGAACCg  >  2:739080/1‑140 (MQ=255)                                                                                                                               ggTCACTGAAAGGCCGAACTCACTATCATTAGCCAGTTCCAGTGCATGTTCTGCATCTTTCGCAATGGTGATTGCCGCAACGGGGCCAAACATTTCTTCCCGAAACGCGGTCATTTCTGGGGTAACATTCGCCAGAACCg  <  1:842663/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                        GCCACTCTTTTTCACGCCACCAAAGGCCACTCGCGCGTCGCTGGCACAATAACCATTGATAAACACCCCACCGCATTCCAGACGTGCCGCCATCTGTCTGGCCTGTGTTTCGTCAGTGGTAAAAATGGTCGCTGAAAGGCCGAACTCACTATCATTAGCCAGTTCCAGTGCATGTTCTGCATCTTTCGCAATGGTGATTGCCGCAACGGGGCCAAACATTTCTTCCCGAAACGCGGTCATTTCTGGGGTAACATTCGCCAGAACCG  >  NC_000913/1613390‑1613655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑