Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,728,350	T→C	S2P (TCC→CCC)	rnt →	RNase T

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,728,350	0	T	C	93.8%	97.0 / ‑2.0	32	S2P (TCC→CCC)	rnt	RNase T
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (19/11);  total (20/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01

CCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGAACTCATCGCGCCCGCATCTTTACTGCATCGACAAGTAATATTTGTCATAATGCGCGCTGCAATTTATCCGTATTAAGAGAATCAGATGTCCGATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAACCGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGG  >  NC_000913/1728222‑1728485                                                                                                                                 |                                                                                                                                        ccGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGAACTCATCGCGCCCGCATCTTTACTGCATCGACAAGTAATATTTGTCATAATGCGCGCTGCAATTTATCCGTATTAAGAGAATCAGATGCCCGATAACGCt                                                                                                                              <  2:576382/140‑1 (MQ=255)                              gCCGGGCGTGAACTCATCGCGCCCGCATCTTTACTGCATCGACAAGTAATATTTGTCATAATGCGCGCTGCAATTTATCCGTATTAAGAGAATCAGATGCCCGATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCg                                                                                                 <  2:648703/140‑1 (MQ=255)                                       gAACTCATCGCGCCCGCATCTTTACTGCATCGACAAGTAATATTTGTCATAATGCGCGCTGCAATTTATCCGTATTAAGAGAATCAGATGCGCGATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTa                                                                                        <  2:431994/140‑1 (MQ=255)                                             aTCGCGCCCGCATCTTTACTGCATCGACAAGTAATATTTGTCATAATGCGCGCTGCAATTTATCCGTATTAAGAGAATCAGATGTCCGATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCtgt                                                                                  >  1:315934/1‑140 (MQ=255)                                                                  cATCGACAAGTAATATTTGTCATAATGCGCGCTGCAATTTATCCGTATTAAGAGAATCAGATGCCCGATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGAt                                                                            <  2:615780/125‑1 (MQ=255)                                                                  cATCGACAAGTAATATTTGTCATAATGCGCGCTGCAATTTATCCGTATTAAGAGAATCAGATGCCCGATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGAt                                                                            >  1:615780/1‑125 (MQ=255)                                                                  cATCGACAAGTAATATTTGTCATAATGCGCGCTGCAATTTATCCGTATTAAGAGAATCAGATGCCCGATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGc                                                             >  1:337331/1‑140 (MQ=255)                                                                    tCGACAAGTAATATTTGTCATAATGCGCGCTGCAATTTATCCGTATTAAGAGAATCAGATGCCCGATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAg                                                              >  1:659407/1‑137 (MQ=255)                                                                    tCGACAAGTAATATTTGTCATAATGCGCGCTGCAATTTATCCGTATTAAGAGAATCAGATGCCCGATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAg                                                              <  2:659407/137‑1 (MQ=255)                                                                       aCAAGTAATATTTGTCATAATGCGCGCTGCAATTTATCCGTATTAAGAGAATCAGATGCCCGATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGAt                                                        >  2:831747/1‑140 (MQ=255)                                                                                  ttGTCATAATGCGCGCTGCAATTTATCCGTATTAAGAGAATCAGATGCCCGATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCaaa                                             >  1:808532/1‑140 (MQ=255)                                                                                    gTCATAATGCGCGCTGCAATTTATCCGTATTAAGAGAATCAGATGCCCGATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAc                                           >  2:574602/1‑140 (MQ=255)                                                                                    gTCATAATGCGCGCTGCAATTTATCCGTATTAAGAGAATCAGATGCCCGATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAc                                           >  2:54061/1‑140 (MQ=255)                                                                                    gTCATAATGCGCGCTGCAATTTATCCGTATTAAGAGAATCAGATGCCCGATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAc                                           >  2:620276/1‑140 (MQ=255)                                                                                         aaTGCGCGCTGCAATTTATCCGTATTAAGAGAATCAGATGCCCGATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAACCGATg                                      <  1:831747/140‑1 (MQ=255)                                                                                          aTGCGCGCTGAATTTTATCCTTATAAAGAAAATCAGATCCCCTATACCCCTCAACTTCCCGGTTTGTGCGCCCTTTTTCGTGTTTTTTATCCTGTTGTGATCGTTTTTAAACCAGCCGGATTTACCGCAAAACCCGATgc                                     <  2:808532/140‑1 (MQ=255)                                                                                            gcgcgcTGCAATTTATCCGTATTAAGAGAATCAGATGCCCGATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAACCGATgcgc                                   <  1:458404/140‑1 (MQ=255)                                                                                               cgcTGCAATTTATCCGTATTAAGAGAATCAGATGCCCGATAACGCTCAACTTACCGGTCTGTGCGACTGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAACCGATGCgctgc                                >  1:793581/1‑140 (MQ=255)                                                                                               cgcTGCAATTTATCCGTATTAAGAGAATCAGATGCCCGATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAACCGATGCgctgc                                >  2:298678/1‑140 (MQ=255)                                                                                               cgcTGCAATTTATCCGTATTAAGAGAATCAGATGCCCGATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAACCGATGCgctgc                                >  2:466614/1‑140 (MQ=255)                                                                                                            ccGTATTAAGAGAATCAGATGCCCGATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAACCGATGCGCTGCTTGAGATTgccgc                   >  2:713905/1‑140 (MQ=255)                                                                                                            ccGTATTAAGAGAATCAGATGCCCGATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAACCGATGCGCTGCTTGAGATTgccgc                   >  1:469525/1‑140 (MQ=255)                                                                                                               tATTAAGAGAATCAGATGCCCGATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAACCGATGCGCTGCTTGAGATTGCCGCCAt                >  2:359073/1‑140 (MQ=255)                                                                                                               tATTAAGAGAATCAGATGCCCGATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAACCGATGCGCTGCTTGAGATTGCCGCCAt                <  1:620276/140‑1 (MQ=255)                                                                                                               tATTAAGAGAATCAGATGCCCGATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCAAAAACCGATGCGCTGCTTGAGATTGCCGCCAt                >  1:154788/1‑140 (MQ=255)                                                                                                                        aaTCAGATGTCCGATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAACCGATGCGCTGCTTGAGATTGCCGCCATCACCCTGaa       <  2:315934/140‑1 (MQ=255)                                                                                                                        aaTCAGATGCCCGATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAACCGATGCGCTGCTTGAGATTGCCGCCATCACCCTGaa       >  1:27882/1‑140 (MQ=255)                                                                                                                         aTCAGATGCCCGATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAACCGATGCGCTGCTTGAGATTGCCGCCATCa              >  2:359997/1‑132 (MQ=255)                                                                                                                         aTCAGATGCCCGATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAACCGATGCGCTGCTTGAGATTGCCGCCATCa              <  1:359997/132‑1 (MQ=255)                                                                                                                         aTCAGATGCCCGATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAACCGATGCGCTGCTTGAGATTGCCGCCATCACCCTGaaa      >  1:589050/1‑140 (MQ=255)                                                                                                                         aTCAGATGCCCGATAACGCTCAACTTACCGGTCTGTGCGACCATTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAACCGATGCGCTGCTTGAGATTGCCGCCATCACCCTGaaa      <  1:399235/140‑1 (MQ=255)                                                                                                                          tCAGATGCCCGATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAACCGATGCGCTGCTTGAGATTGCCGCCATCACCCTGaaaa     >  2:284545/1‑140 (MQ=255)                                                                                                                             gATGCCCGATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAACCGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATgg  <  1:713905/140‑1 (MQ=255)                                                                                                                                 |                                                                                                                                        CCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGAACTCATCGCGCCCGCATCTTTACTGCATCGACAAGTAATATTTGTCATAATGCGCGCTGCAATTTATCCGTATTAAGAGAATCAGATGTCCGATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAACCGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGG  >  NC_000913/1728222‑1728485

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑