Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,835,114	T→C	W103R (TGG→CGG)	ydjZ →	DedA family protein YdjZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,835,114	0	T	C	100.0%	158.4 / NA	44	W103R (TGG→CGG)	ydjZ	DedA family protein YdjZ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/16);  total (28/16)

CGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCGCTACGGCAAACACA  >  NC_000913/1834984‑1835249                                                                                                                                   |                                                                                                                                        cGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGCGGACCAGtt                                                                                                                                >  1:711596/1‑140 (MQ=255)    cgcACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGCGGACCAGTTCGa                                                                                                                             >  1:777211/1‑140 (MQ=255)      cACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGCGGACCAGTTCGATg                                                                                                                           >  2:355035/1‑140 (MQ=255)      cACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGCGTCGCTGTTGGGCGCGTTCTGGGGCGGCTTGCTGTCGCGGACCAGTTCGATg                                                                                                                           >  2:806852/1‑140 (MQ=255)                    gTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGCGGACCAGTTCGATGGCCGGCGCGGCGCt                                                                                                             <  1:359264/140‑1 (MQ=255)                              tgttgATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGCGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCtttttt                                                                                                   <  2:777211/140‑1 (MQ=255)                               gttgATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGCGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTa                                                                                                  >  1:678797/1‑140 (MQ=255)                               gttgATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGCGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTa                                                                                                  >  1:471608/1‑140 (MQ=255)                                     gatTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGCGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCa                                                                                            >  2:167640/1‑140 (MQ=255)                                     gatTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGCGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCa                                                                                            <  2:829360/140‑1 (MQ=255)                                               gCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGCGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATggg                                                                                  >  1:356290/1‑140 (MQ=255)                                                 cATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGCGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGcc                                                                                >  1:151775/1‑140 (MQ=255)                                                 cATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGCGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGcc                                                                                >  1:751460/1‑140 (MQ=255)                                                    tgctgcACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGCGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCcgcg                                                                             <  2:46146/140‑1 (MQ=255)                                                    tgctgcACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGCGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCcgcg                                                                             >  2:379186/1‑140 (MQ=255)                                                         cACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGCGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAgtg                                                                        >  1:269902/1‑140 (MQ=255)                                                           ccGCTACCTGCGTTTTTGATCACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGCGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCACCAGAGTGATGGGCCGCGAAgtggt                                                                      >  2:147912/1‑140 (MQ=255)                                                                aCCTGCGTTTTTGATCACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGGCGCGGACCAGTTCGAAGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATCGGCCGCGAAGTGGTGGaaa                                                                 >  2:385369/1‑140 (MQ=255)                                                                  cTGCGTTTTTGATCACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGCGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGaaaaa                                                               >  2:494716/1‑140 (MQ=255)                                                                    gCGTTTTTGATCACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGCGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAAtt                                                             >  2:650989/1‑140 (MQ=255)                                                                      gTTTTTGATCACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGCGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTaa                                                           >  2:206299/1‑140 (MQ=255)                                                                      gTTTTTGATCACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGCGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTaa                                                           >  2:215388/1‑140 (MQ=255)                                                                          ttGATCACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGCGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCgg                                                       <  1:355035/140‑1 (MQ=255)                                                                            gATCACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGCGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCa                                                     <  2:408721/140‑1 (MQ=255)                                                                               cACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGCGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCaaaa                                                  <  1:650989/140‑1 (MQ=255)                                                                                  cTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGCGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCg                                               >  2:374662/1‑140 (MQ=255)                                                                                  cTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGCGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCACCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCg                                               <  1:147912/140‑1 (MQ=255)                                                                                      gCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGCGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCt                                           <  1:379186/140‑1 (MQ=255)                                                                                       ccAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGCGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCtt                                          >  1:129356/1‑140 (MQ=255)                                                                                       ccAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGCGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCtt                                          <  1:215388/140‑1 (MQ=255)                                                                                       ccAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGCGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCtt                                          <  2:711596/140‑1 (MQ=255)                                                                                            gCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGCGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAg                                     >  1:793806/1‑140 (MQ=255)                                                                                               tCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGCGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTAt                                  >  2:698959/1‑140 (MQ=255)                                                                                                          cgcgTTCTGGGGCGGCTTGCTGTCGCGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCtttt                       <  1:494716/140‑1 (MQ=255)                                                                                                          cgcgTTCTGGGGCGGCTTGCTGTCGCGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCtttt                       >  2:641491/1‑140 (MQ=255)                                                                                                               tCTGGGGCGGCTTGCTGTCGCGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACt                  <  2:129356/140‑1 (MQ=255)                                                                                                                      cggcTTGCTGTCGCGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCGCTACg           <  2:471608/140‑1 (MQ=255)                                                                                                                        gcTTGCTGTCGCGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCGCTACGGc         >  2:502766/1‑140 (MQ=255)                                                                                                                        gcTTGCTGTCGCGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCGCTACGGc         >  2:497769/1‑140 (MQ=255)                                                                                                                        gcTTGCTGTCGCGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCGCTACGGc         >  2:121737/1‑140 (MQ=255)                                                                                                                         cTTGCTGTCGCGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCGCTACg           >  2:506043/1‑137 (MQ=255)                                                                                                                         cTTGCTGTCGCGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCGCTACg           <  1:506043/137‑1 (MQ=255)                                                                                                                           tGCTGTCGCGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCGCTACGGCaaa      >  2:328167/1‑140 (MQ=255)                                                                                                                               gTCGCGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCGCTACGGCAAacaca  <  2:751460/140‑1 (MQ=255)                                                                                                                               gTCGCGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCGCTACGGCAAacaca  <  2:151775/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                        CGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCGCTACGGCAAACACA  >  NC_000913/1834984‑1835249

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑