Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,839,309	T→C	V188A (GTT→GCT)	ynjD →	putative ABC transporter ATP‑binding protein YnjD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,839,309	0	T	C	100.0%	184.2 / NA	50	V188A (GTT→GCT)	ynjD	putative ABC transporter ATP‑binding protein YnjD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/31);  total (19/31)

CGCGCCCTTCTCGCCCAACCAAAAGCGTTACTCCTGGATGAGCCATTCAGCCGTCTTGATGTGGCTCTGCGCGATAATTTTCGCCAGTGGGTGTTCAGCGAAGTTCGCGCCCTGGCGATCCCCGTCGTTCAGGTAACGCACGATCTCCAGGATGTTCCTGCTGATAGTTCTGTTCTGGATATGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACT  >  NC_000913/1839182‑1839441                                                                                                                                |                                                                                                                                     cgcgCCCTTCTCGCCCAACCAAAAGCGTTACTCCTGGATGAGCCATTCAGCCGTCTTGATGTGGCTCTGCGCGATAATTTTCGCCAGTGGGTGTTCAGCGAAGTTCGCGCCCTGGCGATCCCCGTCGCTCAGGTAACGCa                                                                                                                          >  1:31344/1‑140 (MQ=255) cgcgCCCTTCTCGCCCAACCAAAAGCGTTACTCCTGGATGAGCCATTCAGCCGTCTTGATGTGGCTCTGCGCGATAATTTTCGCCAGTGGGTGTTCAGCGAAGTTCGCGCCCTGGCGATCCCCGTCGCTCAGGTAACGCa                                                                                                                          >  1:320084/1‑140 (MQ=255) cgcgCCCTTCTCGCCCAACCAAAAGCGTTACTCCTGGATGAGCCATTCAGCCGTCTTGATGTGGCTCTGCGCGATAATTTTCGCCAGTGGGTGTTCAGCGAAGTTCGCGCCCTGGCGATCCCCGTCGCTCAGGTAACGCa                                                                                                                          >  2:503248/1‑140 (MQ=255) cgcgCCCTTCTCGCCCAACCAAAAGCGTTACTCCTGGATGAGCCATTCAGCCGTCTTGATGTGGCTCTGCGCGATAATTTTCGCCAGTGGGTGTTCAGCGAAGTTCGCGCCCTGGCGATCCCCGTCGCTCAGGTAACGCa                                                                                                                          >  1:379341/1‑140 (MQ=255) cgcgCCCTTCTCGCCCAACCAAAAGCGTTACTCCTGGATGAGCCATTCAGCCGTCTTGATGTGGCTCTGCGCGATAATTTTCGCCAGTGGGTGTTCAGCGAAGTTCGCGCCCTGGCGATCCCCGTCGCTCAGGTAACGCa                                                                                                                          >  1:807311/1‑140 (MQ=255) cgcgCCCTTCTCGCCCAACCAAAAGCGTTACTCCTGGATGAGCCATTCAGCCGTCTTGATGTGGCTCTGCGCGATAATTTTCGCCAGTGGGTGTTCAGCGAAGTTCGCGCCCTGGCGATCCCCGTCGCTCAGGTAACGCa                                                                                                                          <  2:98549/140‑1 (MQ=255)  gcgcCCTTCTCGCCCAACCAAAAGCGTTACTCCTGGATGAGCCATTCAGCCGTCTTGATGTGGCTCTGCGCGATAATTTTCGCCAGTGGGTGTTCAGCGAAGTTCGCGCCCTGGCGATCCCCGTCGCTCAGGTAACGCAc                                                                                                                         <  1:171565/140‑1 (MQ=255)  gcgcCCTTCTCGCCCAACCAAAAGCGTTACTCCTGGATGAGCCATTCAGCCGTCTTGATGTGGCTCTGCGCGATAATTTTCGCCAGTGGGTGTTCAGCGAAGTTCGCGCCCTGGCGATCCCCGTCGCTCAGGTAACGCAc                                                                                                                         <  2:592573/140‑1 (MQ=255)   cgcCCTTCTCGCCCAACCAAAAGCGTTACTCCTGGATGAGCCATTCAGCCGTCTTGATGTGGCTCTGCGCGATAATTTTCGCCAGTGGGTGTTCAGCGAAGTTCGCGCCCTGGCGATCCCCGTCGCTCAGGTAACGCACg                                                                                                                        <  2:31344/140‑1 (MQ=255)   cgcCCTTCTCGCCCAACCAAAAGCGTTACTCCTGGATGAGCCATTCAGCCGTCTTGATGTGGCTCTGCGCGATAATTTTCGCCAGTGGGTGTTCAGCGAAGTTCGCGCCCTGGCGATCCCCGTCGCTCAGGTAACGCACg                                                                                                                        >  2:125227/1‑140 (MQ=255)   cgcCCTTCTCGCCCAACCAAAAGCGTTACTCCTGGATGAGCCATTCAGCCGTCTTGATGTGGCTCTGCGCGATAATTTTCGCCAGTGGGTGTTCAGCGAAGTTCGCGCCCTGGCGATCCCCGTCGCTCAGGTAACGCACg                                                                                                                        >  1:52746/1‑140 (MQ=255)    gcCCTTCTCGCCCAACCAAAAGCGTTACTCCTGGATGAGCCATTCAGCCGTCTTGATGTGGCTCTGCGCGATAATTTTCGCCAGTGGGTGTTCAGCGAAGTTCGCGCCCTGGCGATCCCCGTCGCTCAGGTAACGCACGa                                                                                                                       <  1:761213/140‑1 (MQ=255)             gCCCAACCAAAAGCGTTACTCCTGGATGAGCCATTCAGCCGTCTTGATGTGGCTCTGCGCGATAATTTTCGCCAGTGGGTGTTCAGCGAAGTTCGCGCCCTGGCGATCCCCGTCGCTCAGGTAACGCACGATCTCCAGGa                                                                                                              <  2:320084/140‑1 (MQ=255)                  accaaAAGCGTTACTCCTGGATGAGCCATTCAGCCGTCTTGATGTGGCTCTGCGCGATAATTTTCGCCAGTGGGTGTTCAGCGAAGTTCGCGCCCTGGCGATCCCCGTCGCTCAGGTAACGCACGATCTCCAGGATGTTc                                                                                                         >  1:329671/1‑140 (MQ=255)                  accaaAAGCGTTACTCCTGGATGAGCCATTCAGCCGTCTTGATGTGGCTCTGCGCGATAATTTTCGCCAGTGGGTGTTCAGCGAAGTTCGCGCCCTGGCGATCCCCGTCGCTCAGGTAACGCACGATCTCCAGGATGTTc                                                                                                         >  2:821347/1‑140 (MQ=255)                      aaaGCGTTACTCCTGGATGAGCCATTCAGCCGTCTTGATGTGGCTCTGCGCGATAATTTTCGCCAGTGGGTGTTCAGCGAAGTTCGCGCCCTGGCGATCCCCGTCGCTCAGGTAACGCACGATCTCCAGGATGTTCctgc                                                                                                     <  2:63155/140‑1 (MQ=255)                      aaaGCGTTACTCCTGGATGAGCCATTCAGCCGTCTTGATGTGGCTCTGCGCGATAATTTTCGCCAGTGGGTGTTCAGCGAAGTTCGCGCCCTGGCGATCCCCGTCGCTCAGGTAACGCACGATCTCCAGGATGTTCctgc                                                                                                     <  2:221693/140‑1 (MQ=255)                         gCGTTACTCCTGGATGAGCCATTCAGCCGTCTTGATGTGGCTCTGCGCGATAATTTTCGCCAGTGGGTGTTCAGCGAAGTTCGCGCCCTGGCGATCCCCGTCGCTCAGGTAACGCACGATCTCCAGGATGTTCCTGCTGa                                                                                                  <  2:672479/140‑1 (MQ=255)                           gTTACTCCTGGATGAGCCATTCAGCCGTCTTGATGTGGCTCTGCGCGATAATTTTCGCCAGTGGGTGTTCAGCGAAGTTCGCGCCCTGGCGATCCCCGTCGCTCAGGTAACGCACGATCTCCAGGATGTTCCTGCTGATa                                                                                                >  2:504945/1‑140 (MQ=255)                              aCTCCTGGATGAGCCATTCAGCCGTCTTGATGTGGCTCTGCGCGATAATTTTCGCCAGTGGGTGTTCAGCGAAGTTCGCGCCCTGGCGATCCCCGTCGCTCAGGTAACGCACGATCTCCAGGATGTTCCTGCTGATAgtt                                                                                             <  1:503248/140‑1 (MQ=255)                              aCTCCTGGATGAGCCATTCAGCCGTCTTGATGTGGCTCTGCGCGATAATTTTCGCCAGTGGGTGTTCAGCGAAGTTCGCGCCCTGGCGATCCCCGTCGCTCAGGTAACGCACGATCTCCAGGATGTTCCTGCTGATAgtt                                                                                             <  1:821347/140‑1 (MQ=255)                              aCTCCTGGATGAGCCATTCAGCCGTCTTGATGTGGCTCTGCGCGATAATTTTCGCCAGTGGGTGTTCAGCGAAGTTCGCGCCCTGGCGATCCCCGTCGCTCAGGTAACGCACGATCTCCAGGATGTTCCTGCTGATAgtt                                                                                             <  1:364533/140‑1 (MQ=255)                              aCTCCTGGATGAGCCATTCAGCCGTCTTGATGTGGCTCTGCGCGATAATTTTCGCCAGTGGGTGTTCAGCGAAGTTCGCGCCCTGGCGATCCCCGTCGCTCAGGTAACGCACGATCTCCAGGATGTTCCTGCTGATAgtt                                                                                             <  2:201118/140‑1 (MQ=255)                              aCTCCTGGATGAGCCATTCAGCCGTCTTGATGTGGCTCTGCGCGATAATTTTCGCCAGTGGGTGTTCAGCGAAGTTCGCGCCCTGGCGATCCCCGTCGCTCAGGTAACGCACGATCTCCAGGATGTTCCTGCTGATAgtt                                                                                             <  2:379341/140‑1 (MQ=255)                                tCCTGGATGAGCCATTCAGCCGTCTTGATGTGGCTCTGCGCGATAATTTTCGCCAGTGGGTGTTCAGCGAAGTTCGCGCCCTGGCGATCCCCGTCGCTCAGGTAACGCACGATCTCCAGGATGTTCCTGCTGATAgttct                                                                                           <  1:397454/140‑1 (MQ=255)                                  cTGGATGAGCCATTCAGCCGTCTTGATGTGGCTCTGCGCGATAATTTTCGCCAGTGGGTGTTCAGCGAAGTTCGCGCCCTGGCGATCCCCGTCGCTCAGGTAACGCACGATCTCCAGGATGTTCCTGCTGATAgttctgt                                                                                         <  2:52746/140‑1 (MQ=255)                                         aGCCATTCAGCCGTCTTGATGTGGCTCTGCGCGATAATTTTCGCCAGTGGGTGTTCAGCGAAGTTCGCGCCCTGGCGATCCCCGTCGCTCAGGTAACGCACGATCTCCAGGATGTTCCTGCTGATAgtt                                                                                             >  1:526793/1‑129 (MQ=255)                                         aGCCATTCAGCCGTCTTGATGTGGCTCTGCGCGATAATTTTCGCCAGTGGGTGTTCAGCGAAGTTCGCGCCCTGGCGATCCCCGTCGCTCAGGTAACGCACGATCTCCAGGATGTTCCTGCTGATAgtt                                                                                             <  2:526793/129‑1 (MQ=255)                                         aGCCATTCAGCCGTCTTGATGTGGCTCTGCGCGATAATTTTCGCCAGTGGGTGTTCAGCGAAGTTCGCGCCCTGGCGATCCCCGTCGCTCAGGTAACGCACGATCTCCAGGATGTTCCTGCTGATAGTTCTGTTCTGGat                                                                                  >  2:791823/1‑140 (MQ=255)                                            cATTCAGCCGTCTTGATGTGGCTCTGCGCGATAATTTTCGCCAGTGGGTGTTCAGCGAAGTTCGCGCCCTGGCGATCCCCGTCGCTCAGGTAACGCACGATCTCCAGGATGTTCCTGCTGATAGTTCTGTTCTGGATATg                                                                               <  1:504945/140‑1 (MQ=255)                                               tCAGCCGTCTTGATGTGGCTCTGCGCGATAATTTTCGCCAGTGGGTGTTCAGCGAAGTTCGCGCCCTGGCGATCCCCGTCGCTCAGGTAACGCACGATCTCCAGGATGTTCCTGCTGATAGTTCTGTTCTGGATATGgcg                                                                            <  2:329671/140‑1 (MQ=255)                                                  gCCGTCTTGATGTGGCTCTGCGCGATAATTTTCGCCAGTGGGTGTTCAGCGAAGTTCGCGCCCTGGCGATCCCCGTCGCTCAGGTAACGCACGATCTCCAGGATGTTCCTGCTGATAGTTCTGTTCTGGATATGGCGCAg                                                                         <  2:383078/140‑1 (MQ=255)                                                   ccGTCTTGATGTGGCTCTGCGCGATAATTTTCGCCAGTGGGTGTTCAGCGAAGTTCGCGCCCTGGCGATCCCCGTCGCTCAGGTAACGCACGATCTCCAGGATGTTCCTGCTGATAGTTCTGTTCTGGATATGGCGCAGt                                                                        <  2:289542/140‑1 (MQ=255)                                                   ccGTCTTGATGTGGCTCTGCGCGATAATTTTCGCCAGTGGGTGTTCAGCGAAGTTCGCGCCCTGGCGATCCCCGTCGCTCAGGTAACGCACGATCTCCAGGATGTTCCTGCTGATAGTTCTGTTCTGGATATGGCGCAGt                                                                        >  2:682841/1‑140 (MQ=255)                                                        ttGATGTGGCTCTGCGCGATAATTTTCGCCAGTGGGTGTTCAGCGAAGTTCGCGCCCTGGCGATCCCCGTCGCTCAGGTAACGCACGATCTCCAGGATGTTCCTGCTGATAGTTCTGTTCTGGATATGgcg                                                                            >  1:859738/1‑131 (MQ=255)                                                        ttGATGTGGCTCTGCGCGATAATTTTCGCCAGTGGGTGTTCAGCGAAGTTCGCGCCCTGGCGATCCCCGTCGCTCAGGTAACGCACGATCTCCAGGATGTTCCTGCTGATAGTTCTGTTCTGGATATGgcg                                                                            <  2:859738/131‑1 (MQ=255)                                                        ttGATGTGGCTCTGCGCGATAATTTTCGCCAGTGGGTGTTCAGCGAAGTTCGCGCCCTGGCGATCCCCGTCGCTCAGGTAACGCACGATCTCCAGGATGTTCCTGCTGATAGTTCTGTTCTGGATATGGCGCAGTGGTCa                                                                   >  2:509881/1‑140 (MQ=255)                                                                  tctGCGCGATAATTTTCGCCAGTGGGTGTTCAGCGAAGTTCGCGCCCTGGCGATCCCCGTCGCTCAGGTAACGCACGATCTCCAGGATGTTCCTGCTGATAGTTCTGTTCTGGATATGGCGCAGTGGTCAGAAAATTaca                                                         <  1:628129/140‑1 (MQ=255)                                                                          aTAATTTTCGCCAGTGGGTGTTCAGCGAAGTTCGCGCCCTGGCGATCCCCGTCGCTCAGGTAACGCACGATCTCCAGGATGTTCCTGCTGATAGTTCTGTTCTGGATATGGCGCAGTGGTCAGAAAATTACAACAAACTg                                                 >  2:754303/1‑140 (MQ=255)                                                                                                    gAAGTTCGCGCCCTGGCGATCCCCGTCGCTCAGGTAACGCACGATCTCCAGGATGTTCCTGCTGATAGTTCTGTTCTGGATATGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGc                       >  1:98676/1‑140 (MQ=255)                                                                                                    gAAGTTCGCGCCCTGGCGATCCCCGTCGCTCAGGTAACGCACGATCTCCAGGATGTTCCTGCTGATAGTTCTGTTCTGGATATGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGc                       >  1:708971/1‑140 (MQ=255)                                                                                                    gAAGTTCGCGCCC‑GGCGATCCCCGTCGCTCAGGTAACGCACGATCTCCAGGATGTTCCTGCTGATAGTTCTGTTCTGGATATGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCg                      >  2:489319/1‑140 (MQ=255)                                                                                                      aGTTCGCGCCCTGGCGATCCCCGTCGCTCAGGTAACGCACGATCTCCAGGATGTTCCTGCTGATAGTTCTGTTCTGGATATGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGt                     <  2:98676/140‑1 (MQ=255)                                                                                                      aGTTCGCGCCCTGGCGATCCCCGTCGCTCAGGTAACGCACGATCTCCAGGATGTTCCTGCTGATAGTTCTGTTCTGGATATGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGt                     <  2:807311/140‑1 (MQ=255)                                                                                                      aGTTCGCGCCCTGGCGATCCCCGTCGCTCAGGTAACGCACGATCTCCAGGATGTTCCTGCTGATAGTTCTGTTCTGGATATGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGt                     <  2:282167/140‑1 (MQ=255)                                                                                                      aGTTCGCGCCCTGGCGATCCCCGTCGCTCAGGTAACGCACGATCTCCAGGATGTTCCTGCTGATAGTTCTGTTCTGGATATGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGt                     <  2:708971/140‑1 (MQ=255)                                                                                                      aGTTCGCGCCCTGGCGATCCCCGTCGCTCAGGTAACGCACGATCTCCAGGATGTTCCTGCTGATAGTTCTGTTCTGGATATGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGt                     <  1:125227/140‑1 (MQ=255)                                                                                                                     gATCCCCGTCGCTCAGGTAACGCACGATCTCCAGGATGTTCCTGCTGATAGTTCTGTTCTGGATATGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGgcg      <  1:103890/140‑1 (MQ=255)                                                                                                                         cccGTCGCTCAGGTAACGCACGATCTCCAGGATGTTCCTGCTGATAGTTCTGTTCTGGATATGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACt  <  1:791823/140‑1 (MQ=255)                                                                                                                         cccGTCGCTCAGGTAACGCACGATCTCCAGGATGTTCCTGCTGATAGTTCTGTTCTGGATATGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACt  <  1:418697/140‑1 (MQ=255)                                                                                                                                |                                                                                                                                     CGCGCCCTTCTCGCCCAACCAAAAGCGTTACTCCTGGATGAGCCATTCAGCCGTCTTGATGTGGCTCTGCGCGATAATTTTCGCCAGTGGGTGTTCAGCGAAGTTCGCGCCCTGGCGATCCCCGTCGTTCAGGTAACGCACGATCTCCAGGATGTTCCTGCTGATAGTTCTGTTCTGGATATGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACT  >  NC_000913/1839182‑1839441

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑