Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,870,127	T→C	H391H (CAT→CAC)	yeaH →	DUF444 domain‑containing protein YeaH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,870,127	0	T	C	100.0%	119.2 / NA	33	H391H (CAT→CAC)	yeaH	DUF444 domain‑containing protein YeaH
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/17);  total (16/17)

ACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCG  >  NC_000913/1869991‑1870261                                                                                                                                         |                                                                                                                                       aCGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCACCTg                                                                                                                                     <  2:293707/140‑1 (MQ=255)        tAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCACCTGCAATCTa                                                                                                                              <  1:700493/140‑1 (MQ=255)        tAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCACCTGCAATCTa                                                                                                                              <  1:57234/140‑1 (MQ=255)               gCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCACCTGCAATCTACTTTCGa                                                                                                                       <  2:472551/140‑1 (MQ=255)                       ctctCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCACCTGCAATCTACTTTCGACAACTTTg                                                                                                               <  2:50834/140‑1 (MQ=255)                                ttGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCACCTGCAATCTACTTTCGACAACTTTGCGATgcagc                                                                                                      >  2:783625/1‑140 (MQ=255)                                 tGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCACCTGCAATCTACTTTCGACAACTTTGCGATgcagca                                                                                                     >  2:511386/1‑140 (MQ=255)                                           tCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCACCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGAc                                                                                           <  1:511386/140‑1 (MQ=255)                                            ccTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCACCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGAcc                                                                                          <  1:571343/140‑1 (MQ=255)                                               ggCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCACCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAgg                                                                                       >  1:624527/1‑140 (MQ=255)                                                gCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCACCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGga                                                                                      <  1:712156/140‑1 (MQ=255)                                                gCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATAACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCACCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGga                                                                                      <  1:219995/140‑1 (MQ=255)                                                gCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATAACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCACCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGga                                                                                      >  2:219995/1‑140 (MQ=255)                                                                    tgttCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCACCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTcc                                                                  >  1:553032/1‑140 (MQ=255)                                                                    tgttCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCACCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTcc                                                                  >  2:248742/1‑140 (MQ=255)                                                                           tattaCAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCACCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACt                                                           >  2:347356/1‑140 (MQ=255)                                                                             ttaCAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCACCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGt                                                         >  1:662342/1‑140 (MQ=255)                                                                                cAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCACCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTc                                                      >  2:122851/1‑140 (MQ=255)                                                                                     atatCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCACCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGaa                                                             <  1:141524/128‑1 (MQ=255)                                                                                     atatCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCACCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGaa                                                             >  2:141524/1‑128 (MQ=255)                                                                                     atatCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCACCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATaaa                                                 >  1:365477/1‑140 (MQ=255)                                                                                       atCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCACCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACa                                               <  1:122851/140‑1 (MQ=255)                                                                                                     tcgtGCACATCAGACATTGTGGCGAGAATATGAGCACCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTa                                 <  2:553032/140‑1 (MQ=255)                                                                                                            caTCAGACATTGTGGCGAGAATATGAGCACCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGcaa                          <  1:783625/140‑3 (MQ=255)                                                                                                                      tgtgGCGAGAATATGAGCACCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTAt                   >  2:233158/1‑137 (MQ=255)                                                                                                                      tgtgGCGAGAATATGAGCACCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTAt                   <  1:233158/137‑1 (MQ=255)                                                                                                                      tgtgGCGAGAATATGAGCACCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAg                >  2:105757/1‑140 (MQ=255)                                                                                                                       gtgGCGAGAATATGAGCACCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGc               <  2:365477/140‑1 (MQ=255)                                                                                                                       gtgGCGAGAATATGAGCACCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGc               <  1:228776/140‑1 (MQ=255)                                                                                                                         ggCGAGAATATGAGCACCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCa             >  1:529969/1‑140 (MQ=255)                                                                                                                           cGAGAATATGAGCACCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAgg           <  2:624527/140‑1 (MQ=255)                                                                                                                                atatGAGCACCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCAtt      >  2:61600/1‑140 (MQ=255)                                                                                                                                    gAGCACCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCg  >  1:817906/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       ACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCG  >  NC_000913/1869991‑1870261

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑