Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,873,384	A→G	K448K (AAA→AAG)	dgcJ →	putative diguanylate cyclase DgcJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,873,384	0	A	G	100.0%	171.8 / NA	48	K448K (AAA→AAG)	dgcJ	putative diguanylate cyclase DgcJ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/23);  total (25/23)

CGTTATTAGCTCAGGCGATTAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAACGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGATGAGCGTTTATATGTCAATAAGCAGAACAAAAA  >  NC_000913/1873248‑1873518                                                                                                                                         |                                                                                                                                       cGTTATTAGCTCAGGCGATTAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGt                                                                                                                                     <  2:63780/140‑1 (MQ=255) cGTTATTAGCTCAGGCGATTAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGt                                                                                                                                     <  2:757575/140‑1 (MQ=255)     attaGCTCAGGCGATTAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGc                                                                                                                                 >  2:600850/1‑140 (MQ=255)            cAGGCGATTAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACatat                                                                                                                          >  2:603809/1‑140 (MQ=255)            cAGGCGATTAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGATGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGt                                                                                                                                     >  2:542532/1‑129 (MQ=255)            cAGGCGATTAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGATGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGt                                                                                                                                     <  1:542532/129‑1 (MQ=255)             aGGCGATTAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATc                                                                                                                         >  1:609776/1‑140 (MQ=255)                 gATTAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACGACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCgcgc                                                                                                                     <  1:779514/140‑1 (MQ=255)                 gATTAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCgcgc                                                                                                                     <  1:722631/140‑1 (MQ=255)                   ttAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGcgccg                                                                                                                   <  1:603809/140‑1 (MQ=255)                   ttAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGcgccg                                                                                                                   <  1:600850/140‑1 (MQ=255)                           tCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAaga                                                                                                           >  1:665222/1‑140 (MQ=255)                                   tAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCt                                                                                                   >  1:221286/1‑140 (MQ=255)                                          gATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTc                                                                                            <  2:665222/140‑1 (MQ=255)                                           aTTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTcc                                                                                           >  2:754839/1‑140 (MQ=255)                                                    tCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTAc                                                                                  >  1:581660/1‑140 (MQ=255)                                                     ccGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTc                                                                                            >  1:326777/1‑129 (MQ=255)                                                     ccGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTc                                                                                            <  2:326777/129‑1 (MQ=255)                                                     ccGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTAcg                                                                                 >  1:96316/1‑140 (MQ=255)                                                         cTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGAt                                                                             >  1:657853/1‑140 (MQ=255)                                                          tCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATg                                                                            >  1:2909/1‑140 (MQ=255)                                                           cGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATga                                                                           >  2:833403/1‑140 (MQ=255)                                                           cGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATga                                                                           <  2:221286/140‑1 (MQ=255)                                                              tGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATgaaag                                                                        >  1:528833/1‑140 (MQ=255)                                                              tGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATgaaag                                                                        <  2:245736/140‑1 (MQ=255)                                                              tGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATgaaag                                                                        >  2:838616/1‑140 (MQ=255)                                                                    tGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACg                                                                  <  1:489331/140‑1 (MQ=255)                                                                    tGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACg                                                                  <  2:323589/140‑1 (MQ=255)                                                                      aaTTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGAt                                                                <  2:609776/140‑1 (MQ=255)                                                                             catcatTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTAc                                                         <  2:657853/140‑1 (MQ=255)                                                                               tcatTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACat                                                       <  1:410228/140‑1 (MQ=255)                                                                                catTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACatg                                                      >  1:96498/1‑140 (MQ=255)                                                                                        tCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGtat                                              <  2:96316/140‑1 (MQ=255)                                                                                            ttCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGtat                                              <  1:817621/136‑1 (MQ=255)                                                                                            ttCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGtat                                              >  2:817621/1‑136 (MQ=255)                                                                                             tCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGc                                         >  2:537706/1‑140 (MQ=255)                                                                                             tCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGc                                         >  2:490732/1‑140 (MQ=255)                                                                                             tCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGc                                         >  1:805617/1‑140 (MQ=255)                                                                                             tCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGc                                         >  1:511435/1‑140 (MQ=255)                                                                                             tCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGc                                         >  1:499478/1‑140 (MQ=255)                                                                                                  gccgcAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCg                                    <  1:838616/140‑1 (MQ=255)                                                                                                      cAAATTGCAGCACAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGATGa                                <  1:754839/140‑1 (MQ=255)                                                                                                               gcaCAACTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGATGAGCGTTtata                       <  2:528833/140‑1 (MQ=255)                                                                                                                     cTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGATGAGCGTTTATATGTCaa                 >  1:64949/1‑140 (MQ=255)                                                                                                                     cTGCCTGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGATGAGCGTTTATATGTCaa                 >  2:760885/1‑140 (MQ=255)                                                                                                                          tGAACGTATCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGATGAGCGTTTATATGTCAATAAGc            <  2:837834/140‑1 (MQ=255)                                                                                                                                 aTCGAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGATGAGCGTTTATATGTCAATAAGCAGAACaa     >  2:39895/1‑140 (MQ=255)                                                                                                                                    gAAAAGCGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGATGAGCGTTTATATGTCAATAAGCAGAACaaaaa  <  1:490732/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       CGTTATTAGCTCAGGCGATTAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAACGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGATGAGCGTTTATATGTCAATAAGCAGAACAAAAA  >  NC_000913/1873248‑1873518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑