Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,888,830 A→G V306A (GTT→GCT)  fadD ← fatty acyl‑CoA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,888,8300AG100.0% 152.8 / NA 42V306A (GTT→GCT) fadDfatty acyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/21);  total (21/21)

TCCGGTCAGTTTCACCCAACGCTCTGCCACCACTTGCTGCACTGGCATCCCACCGCCTGCGGAAAGATGCAGACTGGAGAAATCCAGCTGCTGGAACTCTTTATTGTTCAGCAACGCATTGAACAAGGTGTTAACGCCCGTGATAGCGGTAAACGGATATTTCGCTAACTCTTTTACCAACCCTGGAATATCGCGCGGGTTAGTGATAAGCAGGTTCTGCCCACCCAGTTCGATAAACAGCAGGCAGTTAATGGTCAGGGCAA  >  NC_000913/1888697‑1888959
                                                                                                                                     |                                                                                                                                 
tCCGGTCAGTTTCACCCAACGCTCTGCCACCACTTGCTGCACTGGCATCCCACCGCCTGCGGAAAGATGCAGACTGGAGAAATCCAGCTGCTGGAACTCTTTATTGTTCAGCAACGCATTGAACAAGGTGTTAGCGCCCg                                                                                                                             >  1:123163/1‑140 (MQ=255)
tCCGGTCAGTTTCACCCAACGCTCTGCCACCACTTGCTGCACTGGCAT‑CCACCGCCTGCGGAAAGATGCAGACTGGAGAAATCCAGCTGCTGGAACTCTTTATTGTTCAGCAACGCATTGAACAAGGTGTTAGCGCCCGt                                                                                                                            >  1:819953/1‑140 (MQ=255)
           tCACCCAACGCTCTGCCACCACTTGCTGCACTGGCATCCCACCGCCTGCGGAAAGATGCAGACTGGAGAAATCCAGCTGCTGGAACTCTTTATTGTTCAGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTa                                                                                                                  <  2:462763/140‑1 (MQ=255)
               ccAACGCTCTGCCACCACTTGCTGCACTGGCATCCCACCGCCTGCGGAAAGATGCAGACTGGAGAAATCCAGCTGCTGGAACTCTTTATTGTTCAGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACg                                                                                                              >  2:4712/1‑140 (MQ=255)
                    gCTCTGCCACCACTTGCTGCACTGGCATCCCACCGCCTGCGGAAAGATGCAGACTGGAGAAATCCAGCTGCTGGAACTCTTTATTGTTCAGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGatat                                                                                                         <  2:123163/140‑1 (MQ=255)
                    gCTCTGCCACCACTTGCTGCACTGGCATCCCACCGCCTGCGGAAAGATGCAGACTGGAGAAATCCAGCTGCTGGAACTCTTTATTGTTCAGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGatat                                                                                                         <  1:4712/140‑1 (MQ=255)
                             ccacTTGCTGCACTGGCATCCCACCGCCTGCGGAAAGATGCAGACTGGAGAAATCCAGCTGCTGGAACTCTTTATTGTTCAGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGATATTTCGCTAAc                                                                                                >  2:543503/1‑140 (MQ=255)
                              cacTTGCTGCACTGGCATCCCACCGCCTGCGGAAAGATGCAGACTGGAGAAATCCAGCTGCTGGAACTCTTTATTGTTCAGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGATATTTCGCTAAct                                                                                               >  1:482465/1‑140 (MQ=255)
                               acTTGCTGCACTGGCATCCCACCGCCTGCGGAAAGATGCAGACTGGAGAAATCCAGCTGCTGGAACTCTTTATTGTTCAGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGATATTTCGCTAActc                                                                                              <  1:329863/140‑1 (MQ=255)
                                  tgctgcACTGGCATCCCACCGCCTGCGGAAAGATGCAGACTGGAGAAATCCAGCTGCTGGAACTCTTTATTGTTCAGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGATATTTCGCTAACTCttt                                                                                           >  1:139394/1‑140 (MQ=255)
                                     tgcACTGGCATCCCACCGCCTGCGGAAAGATGCAGACTGGAGAAATCCAGCTGCTGGAACTCTTTATTGTTCAGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGATATTTCGCTAACTCTTTTAc                                                                                        <  1:374890/140‑1 (MQ=255)
                                        aCTGGCATCCCACCGCCTGCGGAAAGATGCAGACTGGAGAAATCCAGCTGCTGGAACTCTTTATTGTTAAGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGATATTTCGCTAACTCTTTTACCaa                                                                                     <  2:601158/140‑1 (MQ=255)
                                         cTGGCATCCCACCGCCTGCGGAAAGATGCAGACTGGAGAAATCCAGCTGCTGGAACTCTTTATTGTTCAGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGATATTTCGCTAACTCTTTTACCAAc                                                                                    >  2:63747/1‑140 (MQ=255)
                                               tCCCACCGCCTGCGGAAAGATGCAGACTGGAGAAATCCAGCTGCTGGAACTCTTTATTGTTCAGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGATATTTCGCTAACTCTTTTACTAACCCTGGa                                                                              >  2:419935/1‑140 (MQ=255)
                                                  cACCGCCTGCGGAAAGATGCAGACTGGAGAAATCCAGCTGCTGGAACTCTTTATTGTTCAGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGATATTTCGCTAACTCTTTTACCAACCCTGGAata                                                                           <  1:543503/140‑1 (MQ=255)
                                                      gCCTGCGGAAAGATGCAGACTGGAGAAATCCAGCTGCTGGAACTCTTTATTGTTCAGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGATATTTCGCTAACTCTTTTACCAACCCTGGAATATcgc                                                                       >  2:560701/1‑140 (MQ=255)
                                                      gCCTGCGGAAAGATGCAGACTGGAGAAATCCAGCTGCTGGAACTCTTTATTGTTCAGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGATATTTCGCTAACTCTTTTACCAACCCTGGAATATcgc                                                                       >  1:63614/1‑140 (MQ=255)
                                                       ccTGCGGAAAGATGCAGACTGGAGAAATCCAGCTGCTGGAACTCTTTATTGTTCAGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGATATTTCGCTAACTCTTTTACCAACCCTGGAATATcgcg                                                                      <  1:156219/140‑1 (MQ=255)
                                                        cTGCGGAAAGATGCAGACTGGAGAAATCCAGCTGCTGGAACTCTTTATTGTTCAGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGATATTTCGCTAACTCTTTTACCAACCCTGGAATATcgcgc                                                                     <  2:819953/140‑1 (MQ=255)
                                                           cGGAAAGATGCAGACTGGAGAAATCCAGCTGCTGGAACTCTTTATTGTTCAGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGATATTTCGCTAACTCTTTTACCAACCCTGGAATATCGCGCggg                                                                  >  2:174809/1‑140 (MQ=255)
                                                                aGATGCAGACTGGAGAAATCCAGCTGCTGGAACTCTTTATTGTTCAGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGATATTTCGCTAACTCTTTTACCAACCCTGGAATATCGCGCGGGTTAGt                                                             <  1:419935/140‑1 (MQ=255)
                                                                 gATGCAGACTGGAGAAATCCAGCTGCTGGAACTCTTTATTGTTCAGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGATATTTCGCTAACTCTTTTACCAACCCTGGAATATCGCGCGGGTTAGTg                                                            <  1:106368/140‑1 (MQ=255)
                                                                            gagaAATCCAGCTGCTGGAACTCTTTATTGTTCAGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGATATTTCGCTAACTCTTTTACCAACCCTGGAATATCGCGCGGGTTAGTGATAAGCAGGtt                                                 >  1:616713/1‑140 (MQ=255)
                                                                            gagaAATCCAGCTGCTGGAACTCTTTATTGTTCAGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGATATTTCGCTAACTCTTTTACCAACCCTGGAATATCGCGCGGGTTAGTGATAAGCAGGtt                                                 >  1:573874/1‑140 (MQ=255)
                                                                            gagaAATCCAGCTGCTGGAACTCTTTATTGTTCAGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGATATTTCGCTAACTCTTTTACCAACCCTGGAATATCGCGCGGGTTAGTGATAAGCAGGtt                                                 >  2:808212/1‑140 (MQ=255)
                                                                            gagaAATCCAGCTGCTGGAACTCTTTATTGTTCAGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGATATTTCGCTAACTCTTTTACCAACCCTGGAATATCGCGCGGGTTAGTGATAAGCAGGtt                                                 >  1:856191/1‑140 (MQ=255)
                                                                              gaAATCCAGCTGCTGGAACTCTTTATTGTTCAGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGATATTTCGCTAACTCTTTTACCAACCCTGGAATATCGCGCGGGTTAGTGATAAGCAGGTTCt                                               >  1:93988/1‑140 (MQ=255)
                                                                                aaTCCAGCTGCTGGAACTCTTTATTGTTCAGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGATATTTCGCTAACTCTTTTACCAACCCTGGAATATCGCGCGGGTTAGTGATAAGCAGGTTCTGc                                             <  1:803272/140‑1 (MQ=255)
                                                                                  tCCAGCTGCTGGAACTCTTTATTGTTCAGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGATATTTCGCTAACTCTTTTACCAACCCTGGAATATCGCGCGGGTTAGTGATAAGCAGGTTCTGccc                                           >  2:745683/1‑140 (MQ=255)
                                                                                   ccAGCTGCTGGAACTCTTTATTGTTCAGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGATATTTCGCTAACTCTTTTACCAACCCTGGAATATCGCGCGGGTTAGTGATAAGCAGGTTCTGccca                                          <  2:139394/140‑1 (MQ=255)
                                                                                          ctgGAACTCTTTATTGTTCAGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGATATTTCGCTAACTCTTTTACCAACCCTGGAATATCGCGCGGGTTAGTGATAAGCAGGTTCTGCCCACCCAGtt                                   >  1:606963/1‑140 (MQ=255)
                                                                                            ggAACTCTTTATTGTTCAGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGATATTTCGCTAACTCTTTTACCAACCCTGGAATATCGCGCGGGTTAGTGATAAGCAGGTTCTGCCCACCCAGTTCg                                 <  1:786715/140‑1 (MQ=255)
                                                                                            ggAACTCTTTATTGTTCAGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGATATTTCGCTAACTCTTTTACCAACCCTGGAATATCGCGCGGGTTAGTGATAAGCAGGTTCTGCCCACCCAGTTCg                                 >  1:357284/1‑140 (MQ=255)
                                                                                                       ttGTTCAGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGATATTTCGCTAACTCTTTTACCAACCCTGGAATATCGCGCGGGTTAGTGATAAGCAGGTTCTGCCCACCCAGTTCGATAAAcagcag                      <  1:745683/140‑1 (MQ=255)
                                                                                                         gTTCAGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGATATTTCGCTAACTCTTTTACCAACCCTGGAATATCGCGCGGGTTAGTGATAAGCAGGTTCTGCCCACCCAGTTCGATAAACAgcaggc                    <  2:482465/140‑1 (MQ=255)
                                                                                                         gTTCAGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGATATTTCGCTAACTCTTTTACCAACCCTGGAATATCGCGCGGGTTAGTGATAAGCAGGTTCTGCCCACCCAGTTCGATAAACAgcaggc                    <  1:63747/140‑1 (MQ=255)
                                                                                                         gTTCAGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGATATTTCGCTAACTCTTTTACCAACCCTGGAATATCGCGCGGGTTAGTGATAAGCAGGTTCTGCCCACCCAGTTCGATAAACAgcaggc                    <  1:174809/140‑1 (MQ=255)
                                                                                                             aGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGATATTTCGCTAACTCTTTTACCAACCCTGGAATATCGCGCGGGTTAGTGATAAGCAGGTTCTGCCCACCCAGTTCGATAAACAgcaggc                    >  2:151621/1‑136 (MQ=255)
                                                                                                             aGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGATATTTCGCTAACTCTTTTACCAACCCTGGAATATCGCGCGGGTTAGTGATAAGCAGGTTCTGCCCACCCAGTTCGATAAACAgcaggc                    <  1:151621/136‑1 (MQ=255)
                                                                                                                    cATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGATATTTCGCTAACTCTTTTACCAACCCTGGAATATCGCGCGGGTTAGTGATAAGCAGGTTCTGCCCACCCAGTTCGATAAACAgcaggc                    <  2:121731/129‑1 (MQ=255)
                                                                                                                    cATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGATATTTCGCTAACTCTTTTACCAACCCTGGAATATCGCGCGGGTTAGTGATAAGCAGGTTCTGCCCACCCAGTTCGATAAACAgcaggc                    >  1:121731/1‑129 (MQ=255)
                                                                                                                           cAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGATATTTCGCTAACTCTTTTACCAACCCTGGAATATCGCGCGGGTTAGTGATAAGCAGGTTCTGCCCACCCAGTTCGATAAACAGCAGGCAGTTAATGGTCAGGGCaa  <  1:808212/140‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                 
TCCGGTCAGTTTCACCCAACGCTCTGCCACCACTTGCTGCACTGGCATCCCACCGCCTGCGGAAAGATGCAGACTGGAGAAATCCAGCTGCTGGAACTCTTTATTGTTCAGCAACGCATTGAACAAGGTGTTAACGCCCGTGATAGCGGTAAACGGATATTTCGCTAACTCTTTTACCAACCCTGGAATATCGCGCGGGTTAGTGATAAGCAGGTTCTGCCCACCCAGTTCGATAAACAGCAGGCAGTTAATGGTCAGGGCAA  >  NC_000913/1888697‑1888959

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑