| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,972,742 | A→G | intergenic (‑51/+94) | tar ← / ← cheW | methyl‑accepting chemotaxis protein Tar/chemotaxis protein CheW |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,972,742 | 0 | A | G | 95.7% | 56.8 / ‑6.3 | 23 | intergenic (‑51/+94) | tar/cheW | methyl‑accepting chemotaxis protein Tar/chemotaxis protein CheW |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (13/9); total (13/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.35e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTGCGAATACCCCCAGCACCATTACCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAAC > NC_000913/1972634‑1972866 | gTGCGAATACCCCCAGCACCATTACCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCCCCTTCCTAAAAACAAGTTGATCTCGTTATCGGCAAGGAGGGGGGGAACTTTATTGCTGATGCCACCCGCCGCGaaa < 2:359956/140‑1 (MQ=255) aGCACCATTACCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAAGGAGGGGGGGAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAAc > 2:551769/1‑140 (MQ=255) ttACCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAAGGAGGGGGGGAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATgc > 1:323938/1‑140 (MQ=255) cGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAAGGAGGGGGGGAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGatcatt > 1:157347/1‑139 (MQ=255) gTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAGCAAGTTGATCTCGTTATCGGCAAGGAGGGGGGGAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCg > 2:770475/1‑140 (MQ=255) gACTACGGGGATACGGTTAATCAAAAGGCCCCTTCCTGAAAACAAGTTGATCTCGTTATCGGAAGGGGGGGGGGGAACTTTATTGCTGATGCCCCCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCggg < 2:372699/140‑1 (MQ=255) aCTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAAGGAGGGGGGGAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGt > 2:674757/1‑140 (MQ=255) cgGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAAGGAGGGGGGGAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCAT‑AGGTTCATTTc > 2:426097/1‑140 (MQ=255) cGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAAGGAGGGGGGGAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTg > 1:658749/1‑140 (MQ=255) gTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAAGGAGGGGGGGAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAg < 1:551769/140‑1 (MQ=255) gaGGCCCCTTCCTAAAAACAAGTTGATCTCGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCggg < 1:237650/139‑1 (MQ=255) cTTCCTGAAAACAAGTTGATCTCGTTATCGGCAAGGAGGGGGGGAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTa > 2:758067/1‑140 (MQ=255) cTTCCTGAAAACAAGTTGATCTCGTTATCGGCAAGGAGGGGGGGAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTa > 1:517786/1‑140 (MQ=255) cTTCCTGAAAACAAGTTGATCTCGTTATCGGCAAGGAGGGGGGGAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTa > 2:836645/1‑140 (MQ=255) aaGATGATCTCGTTATCGGCAAGGAGGGGGGGAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGa > 2:788303/1‑140 (MQ=255) aGTTGATCTCGTTATCGGCAGGGAGGGGGGGAACTTTATTGCTGATGCCCCCCGCCGCGAAATTGAAATAAAAAACCTGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGAc < 1:521697/140‑1 (MQ=255) gTTGATCTCGTTATCGGCAAGGGGGGGGGGAATTTTATTGCTGATGCCCCCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGAcg < 2:517786/140‑1 (MQ=255) gATCTCGTTATCGGCAAGGGGGGGGGGAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGAcgccg < 1:770475/140‑1 (MQ=255) gATCTCGTTATCGGCAAGGAGGGGGGGAATTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGAcgccg < 2:658749/140‑1 (MQ=255) gATCTCGTTATCGGCAAGGAGGGGGGGAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGAcgccg < 1:758067/140‑1 (MQ=255) aaTCTCGTTATCGGAAGGGGGGGGGGGAATTTTATTGCTGATGCCCCCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGAcgccg < 1:814717/139‑1 (MQ=255) ttATCGGCAAGGAGGGGGGGAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATc > 2:234749/1‑140 (MQ=255) cGGCAAGGAGGGGGGGAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAAc > 2:606745/1‑140 (MQ=255) | GTGCGAATACCCCCAGCACCATTACCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAAC > NC_000913/1972634‑1972866 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |