Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,050,172	A→G	K1745K (AAA→AAG)	yeeJ →	inverse autotransporter adhesin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,050,172	0	A	G	100.0%	173.1 / NA	48	K1745K (AAA→AAG)	yeeJ	inverse autotransporter adhesin
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/28);  total (20/28)

AAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTAAAGCTCACGTTAACGACC  >  NC_000913/2050037‑2050305                                                                                                                                        |                                                                                                                                      aaaaGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAGCCTa                                                                                                                                   >  2:591945/1‑140 (MQ=255)             ccGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAGCCTACTGAAGTCAtct                                                                                                                       <  1:41822/140‑1 (MQ=255)               gcgGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAGCCTACTGAAGTCATCTcg                                                                                                                     >  1:179649/1‑140 (MQ=255)                cgGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAGCCTACTGAAGTCATCTcgc                                                                                                                    >  1:131535/1‑140 (MQ=255)                     ccTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAGCCTACTGAAGTCATCTCGCGATTa                                                                                                               <  2:467834/140‑1 (MQ=255)                            cACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAGCCTACTGAAGTCATCTCGCGATTACTGAATg                                                                                                        <  1:591945/140‑1 (MQ=255)                                  aaGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAGCCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTg                                                                                                  <  1:215841/140‑1 (MQ=255)                                  aaGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAGCCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTg                                                                                                  <  1:358609/140‑1 (MQ=255)                                  aaGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAGCCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTg                                                                                                  >  1:111467/1‑140 (MQ=255)                                    gcagcaCCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAGCCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCa                                                                                                <  2:131535/140‑1 (MQ=255)                                        caCCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAGCCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATg                                                                                            >  1:853472/1‑140 (MQ=255)                                         aCCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAGCCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGt                                                                                           >  1:591813/1‑140 (MQ=255)                                                ttGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAGCCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTaa                                                                                        >  2:509969/1‑136 (MQ=255)                                                ttGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAGCCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTaa                                                                                        <  1:509969/136‑1 (MQ=255)                                                ttGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAGCCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCt                                                                                    >  1:799154/1‑140 (MQ=255)                                                  gAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAGCCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGc                                                                                  >  2:821780/1‑140 (MQ=255)                                                  gAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAGCCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGc                                                                                  >  1:21911/1‑140 (MQ=255)                                                  gAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAGCCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGc                                                                                  >  2:214984/1‑140 (MQ=255)                                                        gatgatCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAGCCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGAt                                                                            >  2:94725/1‑140 (MQ=255)                                                        gatgatCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAGCCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGAt                                                                            <  1:206719/140‑1 (MQ=255)                                                          tgatCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAGCCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTa                                                                          <  1:149366/140‑1 (MQ=255)                                                          tgatCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAGCCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTa                                                                          <  1:852120/140‑1 (MQ=255)                                                          tgatCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAGCCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTa                                                                          <  2:743461/140‑1 (MQ=255)                                                          tgatCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAGCCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTa                                                                          <  2:664671/140‑1 (MQ=255)                                                          tgatCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAGCCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTa                                                                          <  1:209851/140‑1 (MQ=255)                                                          tgatCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAGCCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTa                                                                          <  1:214984/140‑1 (MQ=255)                                                           gatCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAGCCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTAc                                                                         <  2:799154/140‑1 (MQ=255)                                                           gatCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAGCCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTAc                                                                         <  2:620957/140‑1 (MQ=255)                                                           gatCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAGCCTACTGAAGTCATCTCGCAATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTAc                                                                         <  2:502607/140‑1 (MQ=255)                                                             tCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAGCCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCa                                                                       >  1:818327/1‑140 (MQ=255)                                                                 ggTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAGCCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCt                                                                   >  1:667148/1‑140 (MQ=255)                                                                   tttCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAGCCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTgg                                                                 >  1:727710/1‑140 (MQ=255)                                                                   tttCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAGCCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTgg                                                                 >  1:520894/1‑140 (MQ=255)                                                                   tttCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAGCCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTgg                                                                 <  2:668342/140‑1 (MQ=255)                                                                      cGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAGCCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGgaga                                                              >  2:39935/1‑140 (MQ=255)                                                                       gCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAGCCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGAt                                                             <  2:21911/140‑1 (MQ=255)                                                                       gCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAGCCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGAt                                                             >  1:308028/1‑140 (MQ=255)                                                                            aaTTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAGCCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCgg                                                        <  2:591813/140‑1 (MQ=255)                                                                            aaTTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAGCCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCgg                                                        <  1:821780/140‑1 (MQ=255)                                                                            aaTTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAGCCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCgg                                                        >  1:14916/1‑140 (MQ=255)                                                                             aTTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAGCCTACTGAAGTCATTTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGa                                                       <  2:855851/140‑1 (MQ=255)                                                                             aTTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAGCCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGa                                                       <  2:179649/140‑1 (MQ=255)                                                                             aTTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAGCCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGa                                                       <  1:94725/140‑1 (MQ=255)                                                                                                          gAAAACAGTTTCAGCCTCTCTGGCAGATAAGCCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAg                          <  1:356871/140‑1 (MQ=255)                                                                                                                       gCCTCTCTGGCAGATAAGCCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTAAAGCTCa             <  2:293532/140‑1 (MQ=255)                                                                                                                            tctGGCAGATAAGCCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAg                          <  2:406765/122‑1 (MQ=255)                                                                                                                            tctGGCAGATAAGCCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAg                          >  1:406765/1‑122 (MQ=255)                                                                                                                                  aGATAAGCCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTAAAGCTCACGTTAACGAcc  <  2:111467/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                      AAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTAAAGCTCACGTTAACGACC  >  NC_000913/2050037‑2050305

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑