Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,104,094	A→G	I139T (ATA→ACA)	wbbK ←	putative lipopolysaccharide biosynthesis protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,104,094	0	A	G	100.0%	147.8 / NA	41	I139T (ATA→ACA)	wbbK	putative lipopolysaccharide biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/27);  total (14/27)

GGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATT  >  NC_000913/2103960‑2104218                                                                                                                                       |                                                                                                                             ggCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATGTCAGc                                                                                                                         <  1:37609/140‑1 (MQ=255) ggCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATGTCAGc                                                                                                                         <  2:63816/140‑1 (MQ=255)      ttATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATGTCAGCCCGta                                                                                                                    <  1:365916/140‑1 (MQ=255)         tCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATGTCAGCCCGTATAg                                                                                                                 >  1:571818/1‑140 (MQ=255)                               gACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATGTCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTa                                                                                           <  2:166126/140‑1 (MQ=255)                                 cTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATGTCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAgg                                                                                         <  1:74025/140‑1 (MQ=255)                                    aCAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATGTCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAgg                                                                                         >  2:343667/1‑137 (MQ=255)                                    aCAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATGTCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAgg                                                                                         <  1:343667/137‑1 (MQ=255)                                              tatttatAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATGTCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAAtt                                                                            >  2:431178/1‑140 (MQ=255)                                                  tataGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATGTCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCAc                                                                        >  1:17584/1‑140 (MQ=255)                                                    taGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATGTCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACgg                                                                      >  2:501380/1‑140 (MQ=255)                                                    taGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATGTCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACgg                                                                      >  1:554340/1‑140 (MQ=255)                                                      gAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATGTCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGaa                                                                    >  2:656036/1‑140 (MQ=255)                                                         tatTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATGTCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATaa                                                                 >  2:406395/1‑140 (MQ=255)                                                         tatTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATGTCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATaa                                                                 >  2:434727/1‑140 (MQ=255)                                                            ttCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATGTCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAAt                                                              >  1:87454/1‑140 (MQ=255)                                                               ttGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATGTCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTcc                                                           >  2:440117/1‑140 (MQ=255)                                                                tGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATGTCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCt                                                          <  2:718362/140‑1 (MQ=255)                                                                       tttttCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATGTCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATaaa                                                   <  1:440117/140‑1 (MQ=255)                                                                        ttttCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATGTCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATaaaa                                                  <  2:571818/140‑1 (MQ=255)                                                                        ttttCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATGTCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATaaaa                                                  <  2:17584/140‑1 (MQ=255)                                                                             tttcATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATGTCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTg                                             <  2:220748/140‑1 (MQ=255)                                                                             tttcATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATGTCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTAT‑AAAAGGTGc                                            <  1:406395/140‑1 (MQ=255)                                                                              ttcATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATGTCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGc                                            <  2:518759/140‑1 (MQ=255)                                                                              ttcATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATGTCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGc                                            <  2:554340/140‑1 (MQ=255)                                                                              ttcATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATGTCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGc                                            <  2:712827/140‑1 (MQ=255)                                                                              ttcATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATGTCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGc                                            <  2:97482/140‑1 (MQ=255)                                                                              ttcATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATGTCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGc                                            <  1:840263/140‑1 (MQ=255)                                                                              ttcATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATGTCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGc                                            <  1:656036/140‑1 (MQ=255)                                                                              ttcATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATGTCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGc                                            <  1:501380/140‑1 (MQ=255)                                                                              ttcATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATGTCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGc                                            <  1:434727/140‑1 (MQ=255)                                                                              ttcATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATGTCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGc                                            <  1:133153/140‑1 (MQ=255)                                                                               tcATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATGTCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCa                                           >  1:162726/1‑140 (MQ=255)                                                                                   ccAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATGTCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGt                                       <  2:320198/140‑1 (MQ=255)                                                                                   ccAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATGTCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGt                                       <  2:87454/140‑1 (MQ=255)                                                                                     aGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATGTCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTa                                     <  1:431178/140‑1 (MQ=255)                                                                                       aaTTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATGTCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATg                                   >  1:12830/1‑140 (MQ=255)                                                                                           gttgAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATGTCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACaa                               <  2:679686/140‑1 (MQ=255)                                                                                                        tGCAGTATTTTTTTTAATGTTTATTTTATATGTCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCttt                  >  2:157869/1‑140 (MQ=255)                                                                                                                 ttttttAATGTTTATTTTATATGTCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTga         <  2:362072/140‑1 (MQ=255)                                                                                                                        aTGTTTATTTTATATGTCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACAtt  <  1:369706/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                             GGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATT  >  NC_000913/2103960‑2104218

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑