Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,135,617	T→C	V12V (GTA→GTG)	wzc ←	protein‑tyrosine kinase Wzc

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,135,617	0	T	C	100.0%	209.6 / NA	57	V12V (GTA→GTG)	wzc	protein‑tyrosine kinase Wzc
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/28);  total (29/28)

CTATAAATCGGCGTGGCGAAGAAGGTGTAAACCACGGCACAGAGGGCGAATACGGTGGTGATGCCAATCACCCACCAGCGCGCTTCAATGACGGTGCCGACCAGGCGACCAATATCGATTTCATCACTGCCCGTTACCGGAGCGGCATGTTGTTTTACTTTTTCTGTCATTCTTATACCTGCTCTGCGTTCAATGCCTGCGCCCACTGGCGGGCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTG  >  NC_000913/2135483‑2135745                                                                                                                                       |                                                                                                                                 cTATAAATCTGCGTGGCGAAGAAGGTGTAAACCACGGCACAGAGGGCGAATACGGTGGTGATGCCAATCACCCACCAGCGCGCTTCAATGACGGTGCCGACCAGGCGACCAATATCGATTTCATCACTGCCCGTCACCgg                                                                                                                             >  1:772884/1‑140 (MQ=255)  tataAATCGGCGTGGCGAAGAA‑GTGTAAACCACGGCACAGAGGGCGAATACGGTGGTGATGCCAATCACCCACCAGCGCGCTTCAATGACGGTGCCGACCAGGCGACCAATATCGATTTCATCACTGCCCGTCACCGGAg                                                                                                                           >  1:19644/1‑140 (MQ=255)   ataAATCTGCGTGGCGAAGAAGGTGTAAACCACGGCACAGAGGGCGAATATGGTGGTGATGCCAATCACCCACCAGCGCGCTTCAATGACGGTGCCGACCAGGCGACCAATATCGATTTCATCACTGCCCGTCACCGGAg                                                                                                                           <  2:772884/140‑1 (MQ=255)   ataAATCGGCGTGGCGAAGAAGGTGTAAACCACGGCACAGAGGGCGAATACGGTGGTGATGCCAATCACCCACCAGCGCGCTTCAATGACGGTGCCGACCAGGCGACCAATATCGATTTCATCACTGCCCGTCACCGGAg                                                                                                                           >  1:748036/1‑140 (MQ=255)     aaaTCGGCGTGGCGAAGAAGGTGTAAACCACGGCACAGAGGGCGAATACGGTGGTGATGCCAATCACCCACCAGCGCGCTTCAATGACGGTGCCGACCAGGCGACCAATATCGATTTCATCACTGCCCGTCACCGGAGCg                                                                                                                         <  1:665513/140‑1 (MQ=255)            cGTGGCGAAGAAGGTGTAAACCACGGCACAGAGGGCGAATACGGTGGTGATGCCAATCACCCACCAGCGCGCTTCAATGACGGTGCCGACCAGGCGACCAATATCGATTTCATCACTGCCCGTCACCg                                                                                                                              >  2:459722/1‑128 (MQ=255)            cGTGGCGAAGAAGGTGTAAACCACGGCACAGAGGGCGAATACGGTGGTGATGCCAATCACCCACCAGCGCGCTTCAATGACGGTGCCGACCAGGCGACCAATATCGATTTCATCACTGCCCGTCACCg                                                                                                                              <  1:459722/128‑1 (MQ=255)            cGTGGCGAAGAAGGTGTAAACCACGGCACAGAGGGCGAATACGGTGGTGATGCCAATCACCCACCAGCGCGCTTCAATGACGGTGCCGACCAGGCGACCAATATCGATTTCATCACTGCCCGTCACCGGAGCGGCAtgtt                                                                                                                  >  2:37066/1‑140 (MQ=255)            cGTGGCGAAGAAGGTGTAAACCACGGCACAGAGGGCGAATACGGTGGTGATGCCAATCACCCACCAGCGCGCTTCAATGACGGTGCCGACCAGGCGACCAATATCGATTTCATCACTGCCCGTCACCGGAGCGGCAtgtt                                                                                                                  >  2:316535/1‑140 (MQ=255)            cGTGGCGAAGAAGGTGTAAACCACGGCACAGAGGGCGAATACGGTGGTGATGCCAATCACCCACCAGCGCGCTTCAATGACGGTGCCGACCAGGCGACCAATATCGATTTCATCACTGCCCGTCACCGGAGCGGCAtg                                                                                                                    >  1:697108/1‑138 (MQ=255)            cGTGGCGAAGAAGGTGTAAACCACGGCACAGAGGGCGAATACGGTGGTGATGCCAATCACCCACCAGCGCGCTTCAATGACGGTGCCGACCAGGCGACCAATATCGATTTCATCACTGCCCGTCACCGGAGCGGCAtg                                                                                                                    <  2:697108/138‑1 (MQ=255)            cGTGGCGAAGAAGGTGTAAACCACGGCACAGAGGGCAAATACGGTGGTGATGCCAATCACCCACCAGCGCGCTTCAATGACGGTGCCGACCAGGCGACCAATATCGATTTCATCACTGCCCGTCACCGGGGCGGCAtgtt                                                                                                                  >  1:126344/1‑140 (MQ=255)              tGGCGAAGAAGGTGTAAACCACGGCACAGAGGGCGAATACGGTGGTGATGCCAATCACCCACCAGCGCGCTTCAATGACGGTGCCGACCAGGCGACCAATATCGATTTCATCACTGCCCGTCACCGGAGCGGCAtgttgt                                                                                                                >  1:336198/1‑140 (MQ=255)                  gaagaaGGTGTAAACCACGGCACAGAGGGCGAATACGGTGGTGATGCCAATCACCCACCAGCGCGCTTCAATGACGGTGCCGACCAGGCGACCAATATCGATTTCATCACTGCCCGTCACCGGAGCGGCATGTTGTTTTa                                                                                                            <  1:31476/140‑1 (MQ=255)                          tgtAAACCACGGCACAGAGGGCGAATACGGTGGTGATGCCAATCACCCACCAGCGCGCTTCAATGACGGTGCCGACCAGGCGACCAATATCGATTTCATCACTGCCCGTCACCGGAGCGGCATGTTGTTTTACTTTTTCt                                                                                                    >  1:169089/1‑140 (MQ=255)                            tAAACCACGGCACAGAGGGCGAATACGGTGGTGATGCCAATCACCCACCAGCGCGCTTCAATGACGGTGCCGACCAGGCGACCAATATCGATTTCATCACTGCCCGTCACCGGAGCGGCATGTTGTTTTACTTTTTCTGt                                                                                                  <  2:19644/140‑1 (MQ=255)                            tAAACCACGGCACAGAGGGCGAATACGGTGGTGATGCCAATCACCCACCAGCGCGCTTCAATGACGGTGCCGACCAGGCGACCAATATCGATTTCATCACTGCCCGTCACCGGAGCGGCATGTTGTTTTACTTTTTCTGt                                                                                                  <  2:779269/140‑1 (MQ=255)                             aaaCCACGGCACAGAGGGCGAATACGGTGGTGATGCCAATCACCCACCAGCGCGCTTCAATGACGGTGCCGACCAGGCGACCAATATCGATTTCATCACTGCCCGTCACCGGAGCGGCATGTTGTTTTACTTTTTCTGTc                                                                                                 <  2:686096/140‑1 (MQ=255)                             aaaCCACGGCACAGAGGGCGAATACGGTGGTGATGCCAATCACCCACCAGCGCGCTTCAATGACGGTGCCGACCAGGCGACCAATATCGATTTCATCACTGCCCGTCACCGGAGCGGCATGTTGTTTTACTTTTTCTGTc                                                                                                 <  2:336198/140‑1 (MQ=255)                             aaaCCACGGCACAGAGGGCGAATACGGTGGTGATGCCAATCACCCACCAGCGCGCTTCAATGACGGTGCCGACCAGGCGACCAATATCGATTTCATCACTGCCCGTCACCGGAGCGGCATGTTGTTTTACTTTTTCTGTc                                                                                                 <  2:169089/140‑1 (MQ=255)                                  aCGGCACAGAGGGCGAATACGGTGGTGATGCCAATCACCCACCAGCGCGCTTCAATGACGGTGCCGACCAGGCGACCAATATCGATTTCATCACTGCCCGTCACCGGAGCGGCATGTTGTTTTACTTTTTCTGTCATTCt                                                                                            <  1:645119/140‑1 (MQ=255)                                    ggCACAGAGGGCGAATACGGTGGTGATGCCAATCACCCACCAGCGCGCTTCAATGACGGTGCCGACCAGGCGACCAATATCGATTTCATCACTGCCCGTCACCGGAGCGGCATGTTGTTTTACTTTTTCTGt                                                                                                  >  1:474449/1‑132 (MQ=255)                                    ggCACAGAGGGCGAATACGGTGGTGATGCCAATCACCCACCAGCGCGCTTCAATGACGGTGCCGACCAGGCGACCAATATCGATTTCATCACTGCCCGTCACCGGAGCGGCATGTTGTTTTACTTTTTCTGt                                                                                                  <  2:474449/132‑1 (MQ=255)                                      cacaGAGGGCGAATACGGTGGTGATGCCAATCACCCACCAGCGCGCTTCAATGACGGTGCCGACCAGGCGACCAATATCGATTTCATCACTGCCCGTCACCGGAGCGGCATGTTGTTTTACTTTTTCTGTCATTCTtata                                                                                        >  2:750961/1‑140 (MQ=255)                                         agagGGCGAATACGGTGGTGATGCCAATCACCCTCCAGCGCGCTTCAATGACGGTGCCGACCAGGCGACCAATATCGATTTCATCACTGCCCGTCACCGGAGCGGCATGTTGTTTTACTTTTTCTGTCATTCTTATACCt                                                                                     <  1:316535/140‑1 (MQ=255)                                            gggCGAATACGGTGGTGATGCCAATCACCCACCAGCGCGCTTCAATGACGGTGCCGACCAGGCGACCAATATCGATTTCATCACTGCCCGTCACCGGAGCGGCATGTTGTTTTACTTTTTCTGTCATTCTTATACCTGct                                                                                  <  2:621745/140‑1 (MQ=255)                                                         ggtgATGCCAATCACCCACCAGCGCGCTTCAATGACGGTGCCGACCAGGCGACCAATATCGATTTCATCACTGCCCGTCACCGGAGCGGCATGTTGTTTTACTTTTTCTGTCATTCTTATACCTGCTCTGCGTTCAATGc                                                                     <  1:37066/140‑1 (MQ=255)                                                          gtgATGCCAATCACCCACCAGCGCGCTTCAATGACGGTGCCGACCAGGCGACCAATATCGATTTCATCACTGCCCGTCACCGGAGCGGCATGTTGTTTTACTTTTTCTGTCATTCTTATGCCTGCTCTg                                                                               <  2:515245/129‑1 (MQ=255)                                                          gtgATGCCAATCACCCACCAGCGCGCTTCAATGACGGTGCCGACCAGGCGACCAATATCGATTTCATCACTGCCCGTCACCGGAGCGGCATGTTGTTTTACTTTTTCTGTCATTCTTATACCTGCTCTg                                                                               >  1:65621/1‑129 (MQ=255)                                                          gtgATGCCAATCACCCACCAGCGCGCTTCAATGACGGTGCCGACCAGGCGACCAATATCGATTTCATCACTGCCCGTCACCGGAGCGGCATGTTGTTTTACTTTTTCTGTCATTCTTATACCTGCTCTg                                                                               >  1:515245/1‑129 (MQ=255)                                                          gtgATGCCAATCACCCACCAGCGCGCTTCAATGACGGTGCCGACCAGGCGACCAATATCGATTTCATCACTGCCCGTCACCGGAGCGGCATGTTGTTTTACTTTTTCTGTCATTCTTATACCTGCTCTg                                                                               <  2:65621/129‑1 (MQ=255)                                                             aTGCCAATCACCCACCAGCGCGCTTCAATGACGGTGCCGACCAGGCGACCAATATCGATTTCATCACTGCCCGTCACCGGAGCGGCATGTTGTTTTACTTTTTCTGTCATTCTTATACCTGCTCTGCGTTCAATGCCTgc                                                                 >  2:313440/1‑140 (MQ=255)                                                              tGCCAATCACCCACCAGCGCGCTTCAATGACGGTGCCGACCAGGCGACCAATATCGATTTCATCACTGCCCGTCACCGGAGCGGCATGTTGTTTTACTTTTTCTGTCATTCTTATACCTGCTCTGCGTTCAATGCCTgcg                                                                <  1:750961/140‑1 (MQ=255)                                                                    tCACCCACCAGCGCGCTTCAATGACGGTGCCGACCAGGCGACCAATATCGATTTCATCACTGCCCGTCACCGGAGCGGCATGTTGTTTTACTTTTTCTGTCATTCTTATACCTGCTCTGCGTTCAATGCCTGCGCCCACt                                                          <  2:126344/140‑1 (MQ=255)                                                                               cgcgcTTCAATGACGGTGCCGACCAGGCGACCAATATCGATTTCATCACTGCCCGTCACCGGAGCGGCATGTTGTTTTACTTTTTCTGTCATTCTTATACCTGCTCTGCGTTCAATGCCTGCGCCCACTGGCGGGCAGAc                                               <  2:748036/140‑1 (MQ=255)                                                                               cgcgcTTCAATGACGGTGCCGACCAGGCGACCAATATCGATTTCATCACTGCCCGTCACCGGAGCGGCATGTTGTTTTACTTTTTCTGTCATTCTTATACCTGCTCTGCGTTCAATGCCTGCGCCCACTGGCGGGCAGAc                                               <  1:313440/140‑1 (MQ=255)                                                                               cgcgcTTCAATGACGGTGCCGACCAGGCGACCAATATCGATTTCATCACTGCCCGTCACCGGAGCGGCATGTTGTTTTACTTTTTCTGTCATTCTTATACCTGCTCTGCGTTCAATGCCTGCGCCCACTGGCGGGCAGAc                                               >  1:645027/1‑140 (MQ=255)                                                                               cgcgcTTCAATGACGGTGCCGACCAGGCGACCAATATCGATTTCATCACTGCCCGTCACCGGAGCGGCATGTTGTTTTACTTTTTCTGTCATTCTTATACCTGCTCTGCGTTCAATGCCTGCGCCCACTGGCGGGCAGAc                                               >  2:402642/1‑140 (MQ=255)                                                                                  gcTTCAATGACGGTGCCGACCAGGCGACCAATATCGATTTCATCACTGCCCGTCACCGGAGCGGCATGTTGTTTTACTTTTTCTGTCATTCTTATACCTGCTCTGCGTTCAATGCCTGCGCCCACt                                                          >  2:252291/1‑126 (MQ=255)                                                                                  gcTTCAATGACGGTGCCGACCAGGCGACCAATATCGATTTCATCACTGCCCGTCACCGGAGCGGCATGTTGTTTTACTTTTTCTGTCATTCTTATACCTGCTCTGCGTTCAATGCCTGCGCCCACt                                                          <  1:252291/126‑1 (MQ=255)                                                                                      cAATGACGGTGCCGACCAGGCGACCAATATCGATTTCATCACTGCCCGTCACCGGAGCGGCATGTTGTTTTACTTTTTCTGTCATTCTTATACCTGCTCTGCGTTCAATGCCTGCGCCCACTGGCGGGCAGACCGTTCaa                                        <  2:327525/140‑1 (MQ=255)                                                                                              gTGCCGACCAGGCGACCAATATCGATTTCATCACTGCCCGTCACCGGAGCGGCATGTTGTTTTACTTTTTCTGTCATTCTTATACCTGCTCTGCGTTCAATGCCTGCGCCCACTGGCGGGCAGACCGTTCAAGt                                      >  2:847887/1‑134 (MQ=255)                                                                                              gTGCCGACCAGGCGACCAATATCGATTTCATCACTGCCCGTCACCGGAGCGGCATGTTGTTTTACTTTTTCTGTCATTCTTATACCTGCTCTGCGTTCAATGCCTGCGCCCACTGGCGGGCAGACCGTTCAAGt                                      <  1:847887/134‑1 (MQ=255)                                                                                              gTGCCGACCAGGCGACCAATATCGATTTCATCACTGCCCGTCACCGGAGCGGCATGTTGTTTTACTTTTTCTGTCATTCTTATACCTGCTCTGCGTTCAATGCCTGCGCCCACTGGCGGGCAGACCGTTCAAGTAAtgtg                                >  1:69747/1‑140 (MQ=255)                                                                                                gCCGACCAGGCGACCAATATCGATTTCATCACTGCCCGTCACCGGAGCGGCATGTTGTTTTACTTTTTCTGTCATTCTTATACCTGCTCTGCGTTCAATGCCTGCGCCCACTGGCGGGCAGACCGTTCAAGTAATGTGTa                              >  2:525792/1‑140 (MQ=255)                                                                                                 ccGACCAGGCGACCAATATCGATTTCATCACTGCCCGTCACCGGAGCGGCATGTTGTTTTACTTTTTCTGTCATTCTTATACCTGCTCTGCGTTCAATGCCTGCGCCCACTGGCGGGCAGACCg                                             >  2:556529/1‑124 (MQ=255)                                                                                                 ccGACCAGGCGACCAATATCGATTTCATCACTGCCCGTCACCGGAGCGGCATGTTGTTTTACTTTTTCTGTCATTCTTATACCTGCTCTGCGTTCAATGCCTGCGCCCACTGGCGGGCAGACCg                                             <  1:556529/124‑1 (MQ=255)                                                                                                         gCGACCAATATCGATTTCATCACTGCCCGTCACCGGAGCGGCATGTTGTTTTACTTTTTCTGTCATTCTTATACCTGCTCTGCGTTCAATGCCTGCGCCCACTGGCGGGCAGACCGTTCAAGTAATGTGTACACCGCTGc                     >  1:39997/1‑140 (MQ=255)                                                                                                           gACCAATATCGATTTCATCACTGCCCGTCACCGGAGCGGCATGTTGTTTTACTTTTTCTGTCATTCTTATACCTGCTCTGCGTTCAATGCCTGCGCCCACTGGCGGGCAGACCGTTCAAGTAATGTGTACACCGCTGCaa                   >  1:113487/1‑140 (MQ=255)                                                                                                            aCCAATATCGATTTCATCACTGCCCGTCACCGGAGCGGCATGTTGTTTTACTTTTTCTGTCATTCTTATACCTGCTCTGCGTTCAATGCCTGCGCCCACTGGCGGGCAGACCGTTCAAGTAATGTGTACACCGCTGCaaa                  <  1:271370/140‑1 (MQ=255)                                                                                                                   tCGATTTCATCACTGCCCGTCACCGGAGCGGCATGTTGTTTTACTTTTTCTGTCATTCTTATACCTGCTCTGCGTTCAATGCCTGCGCCCACTGGCGGGCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTcc           >  2:186485/1‑140 (MQ=255)                                                                                                                       tttCATCACTGCCCGTCACCGGAGCGGCATGTTGTTTTACTTTTTCTGTCATTCTTATACCTGCTCTGCGTTCAATGCCTGCGCCCACTGGCGGGCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGc       >  2:337013/1‑140 (MQ=255)                                                                                                                        ttCATCACTGCCCGTCACCGGAGCGGCATGTTGTTTTACTTTTTCTGTCATTCTTATACCTGCTCTGCGTTCAATGCCTGCGCCCACTGGCGGGCAGACCGTTCAAGTAATGTGTACACCGCTGCaa                   <  2:233500/127‑1 (MQ=255)                                                                                                                        ttCATCACTGCCCGTCACCGGAGCGGCATGTTGTTTTACTTTTTCTGTCATTCTTATACCTGCTCTGCGTTCAATGCCTGCGCCCACTGGCGGGCAGACCGTTCAAGTAATGTGTACACCGCTGCaa                   >  1:233500/1‑127 (MQ=255)                                                                                                                           atcaCTGCCCGTCACCGGATCGGCATGTTGTTTTACTTTTTCTGTCATTCTTATACCTGCTCTGCGTTCAATGCCTGCGCCCACTGGCGGGCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCtttt   >  2:267615/1‑140 (MQ=255)                                                                                                                           atcaCTGCCCGTCACCGGAGCGGCATGTTGTTTTACTTTTTCTGTCATTCTTATACCTGCTCTGCGTTCAATGCCTGCGCCCACTGGCGGGCAGACCGTTTAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCtttt   >  2:243658/1‑140 (MQ=255)                                                                                                                            tcaCTGCCCGTCACCGGAGCGGCATGTTGTTTTACTTTTTCTGTCATTCTTATACCTGCTCTGCGTTCAATGCCTGCGCCCACTGGCGGGCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTg  <  1:337013/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                 CTATAAATCGGCGTGGCGAAGAAGGTGTAAACCACGGCACAGAGGGCGAATACGGTGGTGATGCCAATCACCCACCAGCGCGCTTCAATGACGGTGCCGACCAGGCGACCAATATCGATTTCATCACTGCCCGTTACCGGAGCGGCATGTTGTTTTACTTTTTCTGTCATTCTTATACCTGCTCTGCGTTCAATGCCTGCGCCCACTGGCGGGCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTG  >  NC_000913/2135483‑2135745

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑