Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,141,871	A→G	R115R (CGT→CGC)	dcd ←	dCTP deaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,141,871	0	A	G	100.0%	98.6 / NA	28	R115R (CGT→CGC)	dcd	dCTP deaminase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/10);  total (18/10)

AAAGCTCAGCGCACCAATTAACATGCCCGGACGCAGCGCCAGCGGCAGCTTACCGGAGTTGTAGAACTCCAGCACAATGCAACCAGACCAGCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGAACGCCTCGCCCTCGTCGAGAACGATCTCA  >  NC_000913/2141736‑2142003                                                                                                                                        |                                                                                                                                     aaaGCTCAGCGCACCAATTAACATGCCCGGACGCAGCGCCAGCGGCAGCTTACCGGAGTTGTAGAACTCCAGCACAATGCAACCAGACCAGCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGGcgcg                                                                                                                                  <  1:348334/140‑1 (MQ=255)   aGCTCAGCGCACCAATTAACATGCCCGGACGCAGCGCCAGCGGCAGCTTACCGGAGTTGTAGAACTCCAGCACAATGCAACCAGACCAGCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGGCGCGcc                                                                                                                                <  1:423275/140‑1 (MQ=255)        aGCGCACCAATTAACATGCCCGGACGCAGCGCCAGCGGCAGCTTACCGGAGTTGTAGAACTCCAGCACAATGCAACCAGACCAGCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGGCGCGCCAGTGa                                                                                                                           >  1:598015/1‑140 (MQ=255)               cAATTAACATGCCCGGACGCAGCGCCAGCGGCAGCTTACCGGAGTTGTAGAACTCCAGCACAATGCAACCAGACCAGCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGGcgcg                                                                                                                                  <  1:266264/126‑1 (MQ=255)               cAATTAACATGCCCGGACGCAGCGCCAGCGGCAGCTTACCGGAGTTGTAGAACTCCAGCACAATGCAACCAGACCAGCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGGcgcg                                                                                                                                  >  2:266264/1‑126 (MQ=255)                 aTTAACATGCCCGGACGCAGCGCCAGCGGCAGCTTACCGGAGTTGTAGAACTCCAGCACAATGCAACCAGACCAGCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGGCGCGCCAGTGAGGAACGccc                                                                                                                  >  1:762347/1‑140 (MQ=255)                         gCCCGGACGCAGCGCCAGCGGCAGCTTACCGGAGTTGTAGAACTCCAGCACAATGCAACCAGACCAGCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGGCGCGCCAGTGAGGAACGCCCGTccagcc                                                                                                          >  2:419060/1‑140 (MQ=255)                                   aGCGCCAGCGGCAGCTTACCGGAGTTGTAGAACTCCAGCACAATGCAACCAGACCAGCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGGCGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAg                                                                                                >  1:409202/1‑140 (MQ=255)                                   aGCGCCAGCGGCAGCTTACCGGAGTTGTAGAACTCCAGCACAATGCAACCAGACCAGCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGGCGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAg                                                                                                >  1:752197/1‑140 (MQ=255)                                      gcCAGCGGCAGCTTACCGGAGTTGTAGAACTCCAGCACAATGCAACCAGACCAGCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGGCGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAGATc                                                                                             <  2:745545/140‑1 (MQ=255)                                          gCGGCAGCTTACCGGAGTTGTAGAACTCCAGCACAATGCAACCAGACCAGCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGGCGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAGATCGGCt                                                                                         >  2:289956/1‑140 (MQ=255)                                                                              tGCAACCAGACCAGCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGGCGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACcgccagcgcc                                                     >  1:797831/1‑140 (MQ=255)                                                                              tGCAACCAGACCAGCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGGCGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACcgccagcgcc                                                     >  2:671443/1‑140 (MQ=255)                                                                              tGCAACCAGACCAGCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGGCGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACcgccagcgcc                                                     >  1:801897/1‑140 (MQ=255)                                                                              tGCAACCAGACCAGCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGGCGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACcgccagcgcc                                                     >  1:349182/1‑140 (MQ=255)                                                                                      gaccagCCCGGATCGATTCGGTGCGCGGTGACGTGCACCATCAGCCCCAGGCGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTcc                                             >  2:14584/1‑140 (MQ=255)                                                                                      gaccagCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGGCGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACcgccagcgccag                                                   <  1:490957/134‑1 (MQ=255)                                                                                      gaccagCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGGCGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACcgccagcgccag                                                   >  2:490957/1‑134 (MQ=255)                                                                                       accagCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGGCGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCt                                            >  2:249583/1‑140 (MQ=255)                                                                                        ccagCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGGCGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTg                                           >  1:435403/1‑140 (MQ=255)                                                                                        ccagCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGGCGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTg                                           >  1:368769/1‑140 (MQ=255)                                                                                           gCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGGCGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGt                                        <  2:762347/140‑1 (MQ=255)                                                                                                 aTCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGGCGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGAt                                  >  2:355879/1‑140 (MQ=255)                                                                                                                        gCACCATCAGCCCCAGGCGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGAACGCCTCGCCCTCGTCgaga           <  1:419060/140‑1 (MQ=255)                                                                                                                          aCCATCAGCCCCAGGCGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGAACGCCTCGCCCTCGTCGAGAAc         <  1:249583/140‑1 (MQ=255)                                                                                                                              tCAGCCCCAGGCGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGAACGCCTCg                       >  1:592670/1‑122 (MQ=255)                                                                                                                              tCAGCCCCAGGCGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGAACGCCTCg                       <  2:592670/122‑1 (MQ=255)                                                                                                                                 gCCCCAGGCGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGAACGCCTCGCCCTCGTCGAGAACGATCTCa  <  2:598015/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                     AAAGCTCAGCGCACCAATTAACATGCCCGGACGCAGCGCCAGCGGCAGCTTACCGGAGTTGTAGAACTCCAGCACAATGCAACCAGACCAGCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGAACGCCTCGCCCTCGTCGAGAACGATCTCA  >  NC_000913/2141736‑2142003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑