Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,205,386	T→C	V264A (GTC→GCC)	yehL →	putative AAA(+) MoxR family ATPase YehL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,205,386	0	T	C	100.0%	121.6 / NA	34	V264A (GTC→GCC)	yehL	putative AAA(+) MoxR family ATPase YehL
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/16);  total (18/16)

CTTCGATTTTGAAACGGTTTTTCCGATTATGGATTTCGCCCAGGAGTTGGAACTGGTCGCCAGTGCTTCGGCGCGTTTGCTGGCCCATAGCGGTATTCCGCATAAAGTGCCTGACGCGGTACTGGAATTACTGGTCCGCACCTTCCGCGATCTGCGTGCCAACGGCGAAAAGAAAACGTCAATGGATACACTGACAGCGATTATGTCCACCGCTGAAGCCGTGAACGTGGCCCATGCTGTGGGCGTCCGCGCCTGGTTTTTAGCGAATCGC  >  NC_000913/2205252‑2205522                                                                                                                                       |                                                                                                                                         cTTCGATTTTGAAACGGTTTTTCCGATTATGGATTTCGCCCAGGAGTTGGAACTGGTCGCCAGTGCTTCGGCGCGTTTGCTGGCCCATAGCGGTATTCCGCATAAAGTGCCTGACGCGGTACTGGAATTACTGGCCCGCa                                                                                                                                     <  2:44755/140‑1 (MQ=255)  ttCGATTTTGAAACGGTTTTTCCGATTATGGATTTCGCCCAGGAGTTGGAACTGGTCGCCAGTGCTTCGGCGCGTTTGCTGGCCCATAGCGGTATTCCGCATAAAGTGCCTGACGCGGTACTGGAATTACTGGCCCGCAc                                                                                                                                    <  1:154565/140‑1 (MQ=255)      aTTTTGAAACGGTTTTTCCGATTATGGATTTCGCCCAGGAGTTGGAACTGGTCGCCAGTGCTTCGGCGCGTTTGCTGGCCCATAGCGGTATTCCGCATAAAGTGCCTGACGCGGTACTGGAATTACTGGCCCGCAc                                                                                                                                    >  2:660697/1‑136 (MQ=255)      aTTTTGAAACGGTTTTTCCGATTATGGATTTCGCCCAGGAGTTGGAACTGGTCGCCAGTGCTTCGGCGCGTTTGCTGGCCCATAGCGGTATTCCGCATAAAGTGCCTGACGCGGTACTGGAATTACTGGCCCGCAc                                                                                                                                    <  1:660697/136‑1 (MQ=255)          tGAAACGGTTTTTCCGATTATGGATTTCGCCCAGGAGTTGGAACTGGTCGCCAGTGCTTCGGCGCGTTTGCTGGCCCATAGCGGTATTCCGCATAAAGTGCCTGACGCGGTACTGGAATTACTGGCCCGCACCTTCcgcg                                                                                                                            <  2:836472/140‑1 (MQ=255)                      tCCGATTATGGATTTCGCCCAGGAGTTGGAACTGGTCGCCAGTGCTTCGGCGCGTTTGCTGGCCCATAGCGGTATTCCGCATAAAGTGCCTGACGCGGTACTGGAATTACTGGCCCGCACCTTCCGCGATCTGCGTGCCa                                                                                                                >  2:659999/1‑140 (MQ=255)                                gATTTCGCCCAGGAGTTGGAACTGGTCGCCAGTGCTTCGGCGCGTTTGCTGGCCCATAGCGGTATTCCGCATAAAGTGCCTGACGCGGTACTGGAATTACTGGCCCGCACCTTCCGCGATCTGCGTGCCAACGGCg                                                                                                          >  1:699340/1‑136 (MQ=255)                                gATTTCGCCCAGGAGTTGGAACTGGTCGCCAGTGCTTCGGCGCGTTTGCTGGCCCATAGCGGTATTCCGCATAAAGTGCCTGACGCGGTACTGGAATTACTGGCCCGCACCTTCCGCGATCTGCGTGCCAACGGCg                                                                                                          <  2:699340/136‑1 (MQ=255)                                   ttCGCCCAGGAGTTGGAACTGGTCGCCAGTGCTTCGGCGCGTTTGCTGGCCCATAGCGGTATTCCGCATAAAGTGCCTGACGCGGTACTGGAATTACTGGCCCGCACCTTCCGCGATCTGCGTGCCAACGGCgaaaagaa                                                                                                   <  2:503140/140‑1 (MQ=255)                                     cGCCCAGGAGTTGGAACTGGTCGCCAGTGCTTCGGCGCGTTTGCTGGCCCATAGCGGTATTCCGCATAAAGTGCCTGACGCGGTACTGGAATTACTGGCCCGCACCTTCCGCGATCTGCGTGCCAACGGCgaaaagaaaa                                                                                                 >  2:821875/1‑140 (MQ=255)                                         cAGGAGTTGGAACTGGTCGCCAGTGCTTCGGCGCGTTTGCTGGCCCATAGCGGTATTCCGCATAAAGTGCCTGACGCGGTACTGGAATTACTGGCCCGCACCTTCCGCGATCTGCGTGCCAACGGCGAAAAGAAAACGTc                                                                                             <  1:821875/140‑1 (MQ=255)                                           ggAGTTGGAACTGGTCGCCAGTGCTTCGGCGCGTTTGCTGGCCCATAGCGGTATTCCGCATAAAGTGCCTGACGCGGTACTGGAATTACTGGCCCGCACCTTCCGCGATCTGCGTGCCAACGGCGAAAAGAAAACGTCaa                                                                                           <  1:391599/140‑1 (MQ=255)                                             ggTTGGAACTGGTCGCCAGTGCTTCGGCGCGTTTGCTGGCCCATAGCGGTATTCCGCATAAAGTGCCTGACGCGGTACTGGAATTACTGGCCCGCACCTTCCGCGATCTGCGTGCCAACGGCGAAAAGAAAACGTCAATg                                                                                         <  1:601533/139‑1 (MQ=255)                                                        gTCGCCAGTGCTTCGGCGCGTTTGCTGGCCCATAGCGGTATTCCGCATAAAGTGCCTGACGCGGTACTGGAATTACTGGCCCGCACCTTCCGCGATCTGCGTGCCAACGGCGAAAAGAAAACGTCAATGGATACACTGAc                                                                              >  1:840886/1‑140 (MQ=255)                                                        gTCGCCAGTGCTTCGGCGCGTTTGCTGGCCCATAGCGGTATTCCGCATAAAGTGCCTGACGCGGTACTGGAATTACTGGCCCGCACCTTCCGCGATCTGCGTGCCAACGGCGAAAAGAAAACGTCAATGGATACACTGAc                                                                              >  2:166394/1‑140 (MQ=255)                                                               gTGCTTCGGCGCGTTTGCTGGCCCATAGCGGTATTCCGCATAAAGTGCCTGACGCGGTACTGGAATTACTGGCCCGCACCTTCCGCGATCTGCGTGCCAACGGCGAAAAGAAAACGTCAATGGATACACTGACAGCGAtt                                                                       <  1:210041/140‑1 (MQ=255)                                                                  cTTCGGCGCGTTTGCTGGCCCATAGCGGTATTCCGCATAAAGTGCCTGACGCGGTACTGGAATTACTGGCCCGCACCTTCCGCGATCTGCGTGCCAACGGGGAAAAGAAAACGTCAATGGATACACTGACAGCGATTATg                                                                    >  2:623894/1‑140 (MQ=255)                                                                  cTTCGGCGCGTTTGCTGGCCCATAGCGGTATTCCGCATAAAGTGCCTGACGCGGTACTGGAATTACTGGCCCGCACCTTCCGCGATCTGCGTGCCAACGGCGAAAAGAAAACGTCAATGGATACACTGACAGCGATTATg                                                                    >  2:763192/1‑140 (MQ=255)                                                                  cTTCGGCGCGTTTGCTGGCCCATAGCGGTATTCCGCATAAAGTGCCTGACGCGGTACTGGAATTACTGGCCCGCACCTTCCGCGATCTGCGTGCCAACGGCGAAAAGAAAACGTCAATGGATACACTGACAGCGATTATg                                                                    >  2:474231/1‑140 (MQ=255)                                                                  cTTCGGCGCGTTTGCTGGCCCATAGCGGTATTCCGCATAAAGTGCCTGACGCGGTACTGGAATTACTGGCCCGCACCTTCCGCGATCTGCGTGCCAACGGCGAAAAGAAAACGTCAATGGATACACTGACAGCGATTATg                                                                    >  2:562253/1‑140 (MQ=255)                                                                          cgTTTGCTGGCCCATAGCGGTATTCCGCATAAAGTGCCTGACGCGGTACTGGAATTACTGGCCCGCACCTTCCGCGATCTGCGTGCCAACGGCGAAAAGAAAACGTCAATGGATACACTGACAGCGATTATGTCCACCGc                                                            >  2:721000/1‑140 (MQ=255)                                                                                         aGCGGTATTCCGCATAAAGTGCCTGACGCGGTACTGGAATTACTGGCCCGCACCTTCCGCGATCTGCGTGCCAACGGCGAAAAGAAAACGTCAATGGATACACTGACAGCGATTATGTCCACCGCTGAAGCCGTGAACGt                                             <  1:166394/140‑1 (MQ=255)                                                                                         aGCGGTATTCCGCATAAAGTGCCTGACGCGGTACTGGAATTACTGGCCCGCACCTTCCGCGATCTGCGTGCCAACGGCGAAAAGAAAACGTCAATGGATACACTGACAGCGATTATGTCCACCGCTGAAGCCGTGAACGt                                             <  1:659999/140‑1 (MQ=255)                                                                                             gTATTCCGCATAAAGTGCCTGACGCGGTACTGGAATTACTGGCCCGCACCTTCCGCGATCTGCGTGCCAACGGCGAAAAGAAAACGTCAATGGATACACTGACAGCGATTATGTCCACCGCTGAAGCCGTGAACGTGGcc                                         >  2:586987/1‑140 (MQ=255)                                                                                             gTATTCCGCATAAAGTGCCTGACGCGGTACTGGAATTACTGGCCCGCACCTTCCGCGATCTGCGTGCCAACGGCGAAAAGAAAACGTCAATGGATACACTGACAGCGATTATGTCCACCGCTGAAGCCGTGAACGTGGcc                                         >  1:554965/1‑140 (MQ=255)                                                                                                ttCCGCATAAAGTGCCTGACGCGGTACTGGAATTACTGGCCCGCACCTTCCGCGATCTGCGTGCCAACGGCGAAAAGAAAACGTCAATGGATACACTGACAGCGATTATGTCCACCGCTGAAGCCGTGAACGTGGCCCAt                                      >  1:323301/1‑140 (MQ=255)                                                                                                 tCCGCATAAAGTGCCTGACGCGGTACTGGAATTACTGGCCCGCACCTTCCGCGATCTGCGTGCCAACGGCGAAAAGAAAACGTCAATGGATACACTGACAGCGATTATGTCCACCGCTGAAGCCGTGAACGTGGCCCATg                                     <  2:60680/140‑1 (MQ=255)                                                                                                   cGCATAAAGTGCCTGACGCGGTACTGGAATTACTGGCCCGCACCTTCCGCGATCTGCGTGCCAACGGCGAAAAGAAAACGTCAATGGATACACTGACAGCGATTATGTCCACCGCTGAAGCCGTGAACGTGGCCCATGCt                                   >  1:668578/1‑140 (MQ=255)                                                                                                                 gACGCGGTACTGGAATTACTGGCCCGCACCTTCCGCGATCTGCGTGCCAACGGCGAAAAGAAAACGTCAATGGATACACTGACAGCGATTATGTCCACCGCTGAAGCCGTGAACGTGGCCCATGCTGTGGGCGTCCgcgc                     <  2:486263/140‑1 (MQ=255)                                                                                                                      ggTACTGGAATTACTGGCCCGCACCTTCCGCGATCTGCGTGCCAACGGCGAAAAGAAAACGTCAATGGATACACTGACAGCGATTATGTCCACCGCTGAAGCCGTGAACGTGGCCCATGCTGTGGGCGTCCGCGCCTGGt                >  1:795156/1‑140 (MQ=255)                                                                                                                                  aCTGGCCCGCACCTTCCGCGATCTGCGTGCCAACGGCGAAAAGAAAACGTCAATGGATACACTGACAGCGATTATGTCCACCGCTGAAGCCGTGAACGTGGCCCATGCTGTGGGCGTCCGCGCCTGGTTTTTAGCGAATc    >  2:324787/1‑140 (MQ=255)                                                                                                                                  aCTGGCCCGCACCTTCCGCGATCTGCGTGCCAACGGCGAAAAGAAAACGTCAATGGATACACTGACAGCGATTATGTCCACCGCTGAAGCCGTGAACGTGGCCCATGCTGTGGGCGTCCGCGCCTGGTTTTTAGCGAATc    >  1:681950/1‑140 (MQ=255)                                                                                                                                   cTGGCCCGCACCTTCCGCGATCTGCGTGCCAACGGCGAAAAGAAAACGTCAATGGATACACTGACAGCGATTATGTCCACCGCTGAAGCCGTGAACGTGGCCCATGCTGTGGGCGTCCGCGCCTGGTTTTTAGCGAATcg   <  1:586987/140‑1 (MQ=255)                                                                                                                                    tGGCCCGCACCTTCCGCGATCTGCGTGCCAACGGCGAAAAGAAAACGTCAATGGATACACTGACAGCGATTATGTCCACCGCTGAAGCCGTGAACGTGGCCCATGCTGTGGGCGTCCGCGCCTGGTTTTTAGCGAATcgc  <  2:668578/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                         CTTCGATTTTGAAACGGTTTTTCCGATTATGGATTTCGCCCAGGAGTTGGAACTGGTCGCCAGTGCTTCGGCGCGTTTGCTGGCCCATAGCGGTATTCCGCATAAAGTGCCTGACGCGGTACTGGAATTACTGGTCCGCACCTTCCGCGATCTGCGTGCCAACGGCGAAAAGAAAACGTCAATGGATACACTGACAGCGATTATGTCCACCGCTGAAGCCGTGAACGTGGCCCATGCTGTGGGCGTCCGCGCCTGGTTTTTAGCGAATCGC  >  NC_000913/2205252‑2205522

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑